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Acta Prataculturae Sinica ›› 2024, Vol. 33 ›› Issue (6): 165-174.DOI: 10.11686/cyxb2023271

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Identification of seed vigor and evaluation of seed storability in different varieties of oat

Zhao ZHANG1,2,3(), Ying-ying FU4, Hao-wen SUN4, Feng-xue SUN4, Hui-fang YAN1,2,3()   

  1. 1.College of Grassland Science,Qingdao Agricultural University,Qingdao 266109,China
    2.Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta,Qingdao 266109,China
    3.Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach,Qingdao 266109,China
    4.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China
  • Received:2023-08-05 Revised:2023-09-04 Online:2024-06-20 Published:2024-03-20
  • Contact: Hui-fang YAN

Abstract:

This research explored the differences in seed vigor and storability among different varieties of oat and screened germplasm with high vigor and strong storability. We studied seeds of eight varieties of oat and simulated seed storage by the artificial aging method of seed storage at 45 °C and 100% relative humidity. Responses of 11 indicators related to seed germination and seedling growth were compared for 0 day (i.e. non-aged, control) and after four days. The storability of the tested seeds was evaluated using multivariate methods, including principal component analysis, membership function analysis, comprehensive evaluation value determination (D value) and cluster analysis, to generate an aging tolerance coefficient, and a comprehensive aging tolerance coefficient. This provides a theoretical reference data set for the selection of oat germplasm with high vigor and strong storability and the cultivation of new varieties. It was found that germination energy, germination percentage, germination index, seed vigor index, seedling percentage and seedling vigor index all showed a decline trend under artificial aging, while mean germination time was extended. In principal component analysis, the 11 individual measurement indicators were transformed into 3 independent multivariate scores. Data for the comprehensive evaluation value (D value) of storability and cluster analysis, showed that the 8 oat varieties can be classified into four types: Namely those with strong storability, medium storability, weak storability and extremely weak storability. Variety “Baler Ⅱ” was identified as having strong storability and “Haywire” as having extremely weak storability. This study identifies oat varieties with high vigor and strong storability, and provides a basis for the mining of key candidate genes and the creation and utilization of new germplasm.

Key words: oat, seed vigor, artificial aging, storability, membership function