Abstract:

Sucrose transporters （SUT） are critical transmembrane carriers in higher plants， responsible for the long-distance transport of sucrose from source to sink organs， and sucrose loading and unloading. They play vital roles in plant growth， development， and stress resistance. In this study， we employed bioinformatics methods to identify 9 AsSUTs gene family members in the oat （Avena sativa） genome and analyzed their physicochemical properties， gene structures， chromosomal distributions， phylogenetic relationships， promoter cis-elements， and expression patterns under various abiotic stresses. It was found that the encoded AsSUTs proteins ranged in length from 490 to 687 amino acids， with predicted molecular weights （Mw） of 52.51-73.45 kDa and theoretical isoelectric points （pI） of 6.13-9.72. The genes are distributed across eight chromosomes， with promoter elements primarily involved in hormone response， growth and development， and abiotic stress response. The gene and protein structures of AsSUT members exhibit high conservation. Expression pattern analysis revealed that expression levels of AsSUTs family members under drought and alkaline stress were generally higher than under salt stress. Under drought stress， except for AsSUT6， all other AsSUTs family members showed significantly higher expression in stressed leaves compared to normal leaves. Under salt stress， AsSUT1， AsSUT4， and AsSUT6 exhibited significantly higher expression in stressed leaves than in normal leaves， while under alkaline stress， all AsSUTs members except AsSUT7 showed significantly elevated expression in stressed leaves. Notably， AsSUT1 demonstrated stronger responsiveness to various abiotic stress environments， suggesting its potential critical role in oat stress resistance. These findings provide a theoretical foundation for further research on the SUT family members in oats and their response to environmental stresses.

Key words: Avena sativa, sucrose transporter, bioinformatics, adversity stress, gene expression