Identification and expression analysis of EsJRL genes in Elymus sibiricus

doi:10.11686/cyxb2025066

Abstract

Abstract:

The Jacalin-like lectin gene family is widely found in plants. Its members are known to be involved in plant growth and development， responses to adverse environmental conditions， and defense against pests and diseases. Based on the genomedataof Elymus sibiricus， the EsJRL gene family was identified using bioinformatics methods and analyzed to determine the chromosomal distribution， phylogeny， promoter cis-acting elements， and structure of the genes， and the conserved structural domains and physicochemical properties of the putative proteins. The transcript profiles of EsJRL genes in flag leaves at different growth stages and under various stress conditions （exogenous hormones， drought， and low temperature） were analyzed on the basis of RNA-Seq data and by real-time fluorescence quantitative PCR （RT-qPCR） analysis. A total of 84 EsJRL genes were identified from the E. sibiricus genome. They were unevenly distributed at both ends of 13 chromosomes， and the length of the coding sequences ranged from 93 to 1574 bp. Conserved motif analysis revealed the presence of Motif 1， Motif 3， and Motif 7 in 83， 86， and 86 protein sequences， respectively， indicative of a high degree of structural conservation among the EsJRL gene family. Intraspecific covariance analyses revealed 39 duplication events （18 pairs of tandem duplicated genes and 21 pairs of segmental duplicated genes） in the E. sibiricus genome， and a Ka/Ks analysis showed that all duplicated genes have been subject to purifying selection during their evolutionary history. Cis-acting element prediction showed that the promoter regions of EsJRL genes were enriched with functional elements related to response to light， hormones， and growth and development. Gene expression analyses indicated that only 33 EsJRL genes were expressed in the spikes of two E. sibiricus lines， XH04 and XH05， at different growth stages. Only 19 EsJRL genes were expressed in both germplasm materials. The RT-qPCR results indicated that most EsJRL genes were significantly up-regulated under gibberellin， abscisic acid， and salicylic acid treatments （P<0.05）， and a few EsJRL genes were up-regulated in response to drought and low temperature stress. EsJRL28-2 was significantly down-regulated under drought stress and gibberellin treatment （P<0.05）. Only one EsJRL gene， EsJRL43， was significantly down-regulated under low temperature stress （P<0.05）.

Key words: Elymus sibiricus, Jacalin-related lectin （JRL）, bioinformatics, expression analysis, abiotic stresses

Jia-le LIU, Juan QI, Wen-liang LI, Xin LU, Qi YUAN, Ming-jie LI, Ao-long ZHANG, Wang-yi DU. Identification and expression analysis of EsJRL genes in Elymus sibiricus[J]. Acta Prataculturae Sinica, 2026, 35(1): 206-222.

Figures/Tables 10

References 34

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基因号Gene ID	基因名称Gene name	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
Es2StG00000039474.1	EsJRL23	ATAGGTCCTTGCGGTGGTAGTG	TGGTCTCATCATGGTTGACTTCG
Es7HG00000031260.1	EsJRL74	GCCGTTTGGGAAAGAGGATGG	CAGGACCGCCCGTGGAAG
Es3HG00000084164.1	EsJRL32	GGAGGAGCCGAAGCAGCAG	GCGGGAGCACGGTGGTC
Es3StG00000005761.1	EsJRL37	CGGCGTGGTGGTGGTGAG	CGGGAGGGTGAACGGCATG
Es3HG00000084157.2	EsJRL31-2	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084157.1	EsJRL31-1	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084155.1	EsJRL29	ACCGCCGTGAAGCAGCAG	CCGTGAGGACCGCAGAACC
Es1StG00000032567.1	EsJRL14	CTGTCAACCGGCTGCGTACTG	CCTCCACAACTGGCGTGTCTTC
Es1HG00000058078.1	EsJRL8	ACAAGGTGCATCATCTGGGG	TGATGCTGAACTCTCGCCTC
Es3HG00000088654.1	EsJRL35	AGCTCCGAATCCGTGCTATG	TCTTTTCCTCCATTCCCGCC
Es3StG00000010545.1	EsJRL43	ACTCAAGGCGAGCCAGACAGG	ATGAACGACGATGGAGCCAAACTC
Es2StG00000045601.2	EsJRL28-2	GCTTGCCTGCCCTTCGTTCTC	GCTATCGCATGACCACCAGTTCTC
Es1HG00000053830.1	EsJRL6	GCCGCTACAAACCCTTCCTACTAC	AGTGTGTTGGTTGGCGTTGGTAG

基因号Gene ID	基因名称Gene name	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
Es2StG00000039474.1	EsJRL23	ATAGGTCCTTGCGGTGGTAGTG	TGGTCTCATCATGGTTGACTTCG
Es7HG00000031260.1	EsJRL74	GCCGTTTGGGAAAGAGGATGG	CAGGACCGCCCGTGGAAG
Es3HG00000084164.1	EsJRL32	GGAGGAGCCGAAGCAGCAG	GCGGGAGCACGGTGGTC
Es3StG00000005761.1	EsJRL37	CGGCGTGGTGGTGGTGAG	CGGGAGGGTGAACGGCATG
Es3HG00000084157.2	EsJRL31-2	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084157.1	EsJRL31-1	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084155.1	EsJRL29	ACCGCCGTGAAGCAGCAG	CCGTGAGGACCGCAGAACC
Es1StG00000032567.1	EsJRL14	CTGTCAACCGGCTGCGTACTG	CCTCCACAACTGGCGTGTCTTC
Es1HG00000058078.1	EsJRL8	ACAAGGTGCATCATCTGGGG	TGATGCTGAACTCTCGCCTC
Es3HG00000088654.1	EsJRL35	AGCTCCGAATCCGTGCTATG	TCTTTTCCTCCATTCCCGCC
Es3StG00000010545.1	EsJRL43	ACTCAAGGCGAGCCAGACAGG	ATGAACGACGATGGAGCCAAACTC
Es2StG00000045601.2	EsJRL28-2	GCTTGCCTGCCCTTCGTTCTC	GCTATCGCATGACCACCAGTTCTC
Es1HG00000053830.1	EsJRL6	GCCGCTACAAACCCTTCCTACTAC	AGTGTGTTGGTTGGCGTTGGTAG

基因ID Gene ID	基因名 Gene name	氨基酸数目Number of amino acid （aa）	分子质量 Molecular weight （MW， Da）	等电点 Isoelectric point （pI）	不稳定系数 Instability index （II）	脂溶性指数 Aliphatic index （AI）	亲水性平均值 Grand average of hydropathicity （GRAVY）
Es1HG00000053762.1	EsJRL1	144	14897.71	8.93	10.79	75.07	-0.199
Es1HG00000053820.1	EsJRL2	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053821.1	EsJRL3	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053822.1	EsJRL4	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053823.1	EsJRL5	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053830.1	EsJRL6	249	27090.86	8.79	32.09	74.10	-0.160
Es1HG00000057612.1	EsJRL7	589	63979.22	7.09	41.32	64.67	-0.447
Es1HG00000058078.1	EsJRL8	218	23602.69	8.89	46.10	67.94	-0.454
Es1HG00000059055.2	EsJRL9-2	153	15994.03	5.84	27.08	77.12	-0.137
Es1HG00000059055.1	EsJRL9-1	158	16481.60	5.84	30.54	75.32	-0.128
Es1HG00000059056.2	EsJRL10-2	153	16057.13	6.06	32.45	77.78	-0.133
Es1HG00000059056.1	EsJRL10-1	158	16544.70	6.06	35.73	75.95	-0.125
Es1HG00000059057.2	EsJRL11-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059057.1	EsJRL11-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059058.2	EsJRL12-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059058.1	EsJRL12-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059059.2	EsJRL13-2	153	15957.01	6.06	27.99	77.12	-0.090
Es1HG00000059059.1	EsJRL13-1	158	16471.65	6.71	30.94	75.32	-0.103
Es1StG00000032567.1	EsJRL14	152	16247.31	5.40	35.76	94.14	-0.003
Es1StG00000032569.1	EsJRL15	93	10391.95	9.51	29.75	86.88	0.000
Es1StG00000036517.1	EsJRL16	584	63221.47	7.38	37.84	65.58	-0.395
Es1StG00000037075.1	EsJRL17	220	23694.74	9.27	46.56	64.27	-0.502
Es2HG00000060203.1	EsJRL18	309	33637.74	4.87	41.26	77.28	-0.224
Es2HG00000065677.1	EsJRL19	581	65180.76	6.56	45.25	93.80	-0.100
Es2HG00000065745.1	EsJRL20	696	77761.06	7.31	42.89	71.14	-0.422
Es2StG00000039181.1	EsJRL21	155	16561.74	9.06	25.62	76.71	-0.101
Es2StG00000039209.1	EsJRL22	157	16896.14	9.01	18.57	76.43	-0.152
Es2StG00000039474.1	EsJRL23	148	16611.77	5.93	31.73	72.36	-0.511
Es2StG00000039475.1	EsJRL24	298	32302.38	6.31	14.65	72.62	-0.278
Es2StG00000039477.1	EsJRL25	301	32804.94	5.92	21.33	71.26	-0.274
Es2StG00000039478.1	EsJRL26	310	34429.84	8.92	17.46	70.06	-0.327
Es2StG00000039795.1	EsJRL27	243	26449.77	7.01	38.77	75.84	-0.272
Es2StG00000045601.1	EsJRL28-1	494	55341.59	6.22	44.25	96.68	-0.030
Es2StG00000045601.2	EsJRL28-2	581	65047.63	6.35	45.09	94.96	-0.071
Es3HG00000084155.1	EsJRL29	325	34920.95	7.53	38.87	75.23	-0.129
Es3HG00000084156.1	EsJRL30	244	26216.12	7.54	44.28	79.84	0.032
Es3HG00000084157.1	EsJRL31-1	145	15447.50	6.83	38.05	73.17	-0.194
Es3HG00000084157.2	EsJRL31-2	139	14811.70	6.28	37.12	76.33	-0.188
Es3HG00000084164.1	EsJRL32	244	25959.49	8.48	40.17	74.30	-0.431
Es3HG00000084173.1	EsJRL33	166	18209.30	5.53	42.25	70.30	-0.452
Es3HG00000087351.1	EsJRL34	451	48576.97	9.23	24.63	71.33	-0.276
Es3HG00000088654.1	EsJRL35	317	34442.01	6.66	19.20	82.11	-0.150
Es3StG00000005754.1	EsJRL36	141	15118.15	6.06	39.42	75.96	-0.274
Es3StG00000005761.1	EsJRL37	145	15697.80	6.97	43.98	70.48	-0.220
Es3StG00000005767.1	EsJRL38	332	35598.66	7.07	41.03	78.34	-0.105
Es3StG00000006647.1	EsJRL39	155	16763.02	9.34	28.10	74.84	-0.153
Es3StG00000006682.1	EsJRL40	157	16960.14	7.90	20.70	75.22	-0.196
Es3StG00000007172.1	EsJRL41	103	11180.64	5.29	27.97	74.76	-0.333
Es3StG00000009097.1	EsJRL42	451	48623.73	9.06	22.92	69.58	-0.345
Es3StG00000010545.1	EsJRL43	317	34353.81	7.07	21.78	80.85	-0.183
Es4HG00000066395.1	EsJRL44	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066397.1	EsJRL45	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066399.1	EsJRL46	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066400.1	EsJRL47	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066401.1	EsJRL48	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066403.1	EsJRL49	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066404.1	EsJRL50	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066405.1	EsJRL51	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066407.1	EsJRL52	147	16145.98	5.50	43.69	70.27	-0.458
Es4HG00000067604.1	EsJRL53	149	16142.07	5.07	35.89	77.79	-0.303
Es4HG00000071276.1	EsJRL54-1	919	101893.84	5.99	40.98	85.55	-0.210
Es4HG00000071276.2	EsJRL54-2	989	109609.44	6.20	39.14	83.83	-0.230
Es4StG00000000171.1	EsJRL55	196	20629.91	7.78	29.02	59.13	-0.433
Es4StG00000000209.1	EsJRL56	235	24515.95	4.76	32.34	63.87	-0.177
Es4StG00000000300.1	EsJRL57	180	18897.27	5.42	30.39	80.22	-0.051
Es4StG00000000301.1	EsJRL58	169	17764.90	4.75	32.38	83.08	-0.039
Es4StG00000004303.1	EsJRL59	301	31898.48	6.30	29.27	68.87	-0.225
Es5HG00000074467.1	EsJRL60	304	32991.16	5.16	29.49	78.88	-0.129
Es5HG00000077065.1	EsJRL61	1010	109900.74	5.64	31.82	87.95	-0.140
Es5StG00000012697.1	EsJRL62	504	57157.44	5.28	43.36	82.52	-0.385
Es5StG00000012895.1	EsJRL63	148	15890.99	6.65	34.07	83.51	-0.185
Es5StG00000018974.1	EsJRL64	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018980.1	EsJRL65	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018983.1	EsJRL66	341	37258.82	5.80	16.13	71.99	-0.283
Es6HG00000078673.1	EsJRL67-1	183	19167.51	5.12	34.72	78.36	-0.072
Es6HG00000078673.2	EsJRL67-2	207	21931.69	4.89	35.03	79.13	-0.075
Es6HG00000078921.1	EsJRL68	319	34380.79	8.87	26.94	75.11	-0.186
Es6HG00000079987.1	EsJRL69	321	34277.48	9.88	35.74	74.24	-0.286
Es7HG00000027580.1	EsJRL70	155	16735.84	5.03	29.45	80.45	-0.127
Es7HG00000028732.1	EsJRL71	155	16795.83	6.97	43.97	67.87	-0.393
Es7HG00000030707.1	EsJRL72	328	35853.54	6.06	21.98	77.56	-0.200
Es7HG00000030708.1	EsJRL73	346	37760.64	6.20	22.32	75.23	-0.225
Es7HG00000031260.1	EsJRL74	168	18260.90	8.45	32.02	80.60	-0.238
Es7HG00000032129.1	EsJRL75	310	33630.75	6.14	18.99	76.42	-0.253
Es7HG00000032133.1	EsJRL76	311	34065.22	6.12	26.63	78.01	-0.283
Es7StG00000046382.1	EsJRL77	1574	178373.74	7.92	44.52	88.70	-0.348
Es7StG00000046394.1	EsJRL78	906	101125.34	5.71	39.59	76.05	-0.298
Es7StG00000048488.1	EsJRL79	162	17437.63	5.07	31.44	80.00	-0.168
Es7StG00000049687.1	EsJRL80-1	155	16781.84	6.50	44.16	69.74	-0.370
Es7StG00000049687.2	EsJRL80-2	148	15999.87	6.18	43.69	68.45	-0.386
Es7StG00000052256.1	EsJRL81	307	32583.53	6.10	21.36	79.09	-0.144
Es7StG00000052743.1	EsJRL82	342	36912.21	6.79	30.06	80.96	-0.018
Es7StG00000053443.1	EsJRL83	93	9714.84	5.38	21.86	66.13	0.006
Es7StG00000053444.1	EsJRL84	298	32035.85	5.80	30.58	66.41	-0.343

基因ID Gene ID	基因名 Gene name	氨基酸数目Number of amino acid （aa）	分子质量 Molecular weight （MW， Da）	等电点 Isoelectric point （pI）	不稳定系数 Instability index （II）	脂溶性指数 Aliphatic index （AI）	亲水性平均值 Grand average of hydropathicity （GRAVY）
Es1HG00000053762.1	EsJRL1	144	14897.71	8.93	10.79	75.07	-0.199
Es1HG00000053820.1	EsJRL2	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053821.1	EsJRL3	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053822.1	EsJRL4	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053823.1	EsJRL5	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053830.1	EsJRL6	249	27090.86	8.79	32.09	74.10	-0.160
Es1HG00000057612.1	EsJRL7	589	63979.22	7.09	41.32	64.67	-0.447
Es1HG00000058078.1	EsJRL8	218	23602.69	8.89	46.10	67.94	-0.454
Es1HG00000059055.2	EsJRL9-2	153	15994.03	5.84	27.08	77.12	-0.137
Es1HG00000059055.1	EsJRL9-1	158	16481.60	5.84	30.54	75.32	-0.128
Es1HG00000059056.2	EsJRL10-2	153	16057.13	6.06	32.45	77.78	-0.133
Es1HG00000059056.1	EsJRL10-1	158	16544.70	6.06	35.73	75.95	-0.125
Es1HG00000059057.2	EsJRL11-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059057.1	EsJRL11-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059058.2	EsJRL12-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059058.1	EsJRL12-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059059.2	EsJRL13-2	153	15957.01	6.06	27.99	77.12	-0.090
Es1HG00000059059.1	EsJRL13-1	158	16471.65	6.71	30.94	75.32	-0.103
Es1StG00000032567.1	EsJRL14	152	16247.31	5.40	35.76	94.14	-0.003
Es1StG00000032569.1	EsJRL15	93	10391.95	9.51	29.75	86.88	0.000
Es1StG00000036517.1	EsJRL16	584	63221.47	7.38	37.84	65.58	-0.395
Es1StG00000037075.1	EsJRL17	220	23694.74	9.27	46.56	64.27	-0.502
Es2HG00000060203.1	EsJRL18	309	33637.74	4.87	41.26	77.28	-0.224
Es2HG00000065677.1	EsJRL19	581	65180.76	6.56	45.25	93.80	-0.100
Es2HG00000065745.1	EsJRL20	696	77761.06	7.31	42.89	71.14	-0.422
Es2StG00000039181.1	EsJRL21	155	16561.74	9.06	25.62	76.71	-0.101
Es2StG00000039209.1	EsJRL22	157	16896.14	9.01	18.57	76.43	-0.152
Es2StG00000039474.1	EsJRL23	148	16611.77	5.93	31.73	72.36	-0.511
Es2StG00000039475.1	EsJRL24	298	32302.38	6.31	14.65	72.62	-0.278
Es2StG00000039477.1	EsJRL25	301	32804.94	5.92	21.33	71.26	-0.274
Es2StG00000039478.1	EsJRL26	310	34429.84	8.92	17.46	70.06	-0.327
Es2StG00000039795.1	EsJRL27	243	26449.77	7.01	38.77	75.84	-0.272
Es2StG00000045601.1	EsJRL28-1	494	55341.59	6.22	44.25	96.68	-0.030
Es2StG00000045601.2	EsJRL28-2	581	65047.63	6.35	45.09	94.96	-0.071
Es3HG00000084155.1	EsJRL29	325	34920.95	7.53	38.87	75.23	-0.129
Es3HG00000084156.1	EsJRL30	244	26216.12	7.54	44.28	79.84	0.032
Es3HG00000084157.1	EsJRL31-1	145	15447.50	6.83	38.05	73.17	-0.194
Es3HG00000084157.2	EsJRL31-2	139	14811.70	6.28	37.12	76.33	-0.188
Es3HG00000084164.1	EsJRL32	244	25959.49	8.48	40.17	74.30	-0.431
Es3HG00000084173.1	EsJRL33	166	18209.30	5.53	42.25	70.30	-0.452
Es3HG00000087351.1	EsJRL34	451	48576.97	9.23	24.63	71.33	-0.276
Es3HG00000088654.1	EsJRL35	317	34442.01	6.66	19.20	82.11	-0.150
Es3StG00000005754.1	EsJRL36	141	15118.15	6.06	39.42	75.96	-0.274
Es3StG00000005761.1	EsJRL37	145	15697.80	6.97	43.98	70.48	-0.220
Es3StG00000005767.1	EsJRL38	332	35598.66	7.07	41.03	78.34	-0.105
Es3StG00000006647.1	EsJRL39	155	16763.02	9.34	28.10	74.84	-0.153
Es3StG00000006682.1	EsJRL40	157	16960.14	7.90	20.70	75.22	-0.196
Es3StG00000007172.1	EsJRL41	103	11180.64	5.29	27.97	74.76	-0.333
Es3StG00000009097.1	EsJRL42	451	48623.73	9.06	22.92	69.58	-0.345
Es3StG00000010545.1	EsJRL43	317	34353.81	7.07	21.78	80.85	-0.183
Es4HG00000066395.1	EsJRL44	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066397.1	EsJRL45	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066399.1	EsJRL46	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066400.1	EsJRL47	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066401.1	EsJRL48	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066403.1	EsJRL49	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066404.1	EsJRL50	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066405.1	EsJRL51	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066407.1	EsJRL52	147	16145.98	5.50	43.69	70.27	-0.458
Es4HG00000067604.1	EsJRL53	149	16142.07	5.07	35.89	77.79	-0.303
Es4HG00000071276.1	EsJRL54-1	919	101893.84	5.99	40.98	85.55	-0.210
Es4HG00000071276.2	EsJRL54-2	989	109609.44	6.20	39.14	83.83	-0.230
Es4StG00000000171.1	EsJRL55	196	20629.91	7.78	29.02	59.13	-0.433
Es4StG00000000209.1	EsJRL56	235	24515.95	4.76	32.34	63.87	-0.177
Es4StG00000000300.1	EsJRL57	180	18897.27	5.42	30.39	80.22	-0.051
Es4StG00000000301.1	EsJRL58	169	17764.90	4.75	32.38	83.08	-0.039
Es4StG00000004303.1	EsJRL59	301	31898.48	6.30	29.27	68.87	-0.225
Es5HG00000074467.1	EsJRL60	304	32991.16	5.16	29.49	78.88	-0.129
Es5HG00000077065.1	EsJRL61	1010	109900.74	5.64	31.82	87.95	-0.140
Es5StG00000012697.1	EsJRL62	504	57157.44	5.28	43.36	82.52	-0.385
Es5StG00000012895.1	EsJRL63	148	15890.99	6.65	34.07	83.51	-0.185
Es5StG00000018974.1	EsJRL64	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018980.1	EsJRL65	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018983.1	EsJRL66	341	37258.82	5.80	16.13	71.99	-0.283
Es6HG00000078673.1	EsJRL67-1	183	19167.51	5.12	34.72	78.36	-0.072
Es6HG00000078673.2	EsJRL67-2	207	21931.69	4.89	35.03	79.13	-0.075
Es6HG00000078921.1	EsJRL68	319	34380.79	8.87	26.94	75.11	-0.186
Es6HG00000079987.1	EsJRL69	321	34277.48	9.88	35.74	74.24	-0.286
Es7HG00000027580.1	EsJRL70	155	16735.84	5.03	29.45	80.45	-0.127
Es7HG00000028732.1	EsJRL71	155	16795.83	6.97	43.97	67.87	-0.393
Es7HG00000030707.1	EsJRL72	328	35853.54	6.06	21.98	77.56	-0.200
Es7HG00000030708.1	EsJRL73	346	37760.64	6.20	22.32	75.23	-0.225
Es7HG00000031260.1	EsJRL74	168	18260.90	8.45	32.02	80.60	-0.238
Es7HG00000032129.1	EsJRL75	310	33630.75	6.14	18.99	76.42	-0.253
Es7HG00000032133.1	EsJRL76	311	34065.22	6.12	26.63	78.01	-0.283
Es7StG00000046382.1	EsJRL77	1574	178373.74	7.92	44.52	88.70	-0.348
Es7StG00000046394.1	EsJRL78	906	101125.34	5.71	39.59	76.05	-0.298
Es7StG00000048488.1	EsJRL79	162	17437.63	5.07	31.44	80.00	-0.168
Es7StG00000049687.1	EsJRL80-1	155	16781.84	6.50	44.16	69.74	-0.370
Es7StG00000049687.2	EsJRL80-2	148	15999.87	6.18	43.69	68.45	-0.386
Es7StG00000052256.1	EsJRL81	307	32583.53	6.10	21.36	79.09	-0.144
Es7StG00000052743.1	EsJRL82	342	36912.21	6.79	30.06	80.96	-0.018
Es7StG00000053443.1	EsJRL83	93	9714.84	5.38	21.86	66.13	0.006
Es7StG00000053444.1	EsJRL84	298	32035.85	5.80	30.58	66.41	-0.343

同源基因 Homologous gene	非同义替代率 Non-synonymous substitution （Ka）	同义替代率 Synonymous substitution （Ks）	非同义替代率/同义替代率Ka/Ks	同源基因 Homologous gene	非同义替代率 Non-synonymous substitution （Ka）	同义替代率 Synonymous substitution （Ks）	非同义替代率/同义替代率Ka/Ks
EsJRL8~EsJRL17	0.031309	0.125153	0.250166	EsJRL34~EsJRL42	0.020869	0.160252	0.130227
EsJRL7~EsJRL16	0.015508	0.139083	0.111505	EsJRL35~EsJRL43	0.031225	0.063006	0.495590
EsJRL1~EsJRL14	0.415929	0.577628	0.720063	EsJRL33~EsJRL36	0.314220	0.878862	0.357530
EsJRL7~EsJRL34	0.301874	0.666092	0.453202	EsJRL33~EsJRL37	0.384634	1.148341	0.334947
EsJRL7~EsJRL42	0.305636	0.674034	0.453443	EsJRL29~EsJRL38	0.054512	0.129426	0.421181
EsJRL16~EsJRL34	0.308583	0.719593	0.428830	EsJRL29~EsJRL37	0.256467	0.638630	0.401589
EsJRL16~EsJRL42	0.284311	0.709209	0.400884	EsJRL55~EsJRL56	0.350654	0.538459	0.651218
EsJRL18~EsJRL24	0.464964	1.109302	0.419150	EsJRL57~EsJRL67	0.089606	0.146610	0.611185
EsJRL19~EsJRL28	0.016781	0.034327	0.488853	EsJRL70~EsJRL79	0.052291	0.106226	0.492263
EsJRL21~EsJRL39	0.020150	0.104351	0.193097	EsJRL71~EsJRL80	0.027931	0.084786	0.329428
EsJRL22~EsJRL40	0.019738	0.085265	0.231489