Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus

Abstract

Abstract: Gliadin markers based on A-PAGE were used to analyze genetic diversity and genetic relationships among eighty-six Elymus sibiricus accessions from Asia and North America. A total of 52 gliadin bands were scored, of which 47 were polymorphic (90.4%). The generated similarity matrix showed that the genetic diversity within the accessions was fairly high (average similarity index=0.373). Similarity values among the accessions ranged from 0.108 and 0.952. Similar results were obtained when genetic diversity was estimated using the Shannon index of diversity. The total genetic diversity across all accessions was 0.460. The proportion of variation explained by within geographic groups and between geographic groups diversity was 0.559 and 0.442, respectively. Cluster analysis showed a clear demarcation between accessions from the Qinghai-Tibetan Plateau, China and the others as separate groups. The extent of genetic diversity and genetic relationship among accessions is discussed. The clustering pattern probably depended on geographic origin and ecological adaptability of the accessions.

CLC Number:

MA Xiao, CHEN Shi-yong, ZHANG Xin-quan, ZHOU Yong-hong, BAI Shi-qie, LIU Wei. Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus[J]. Acta Prataculturae Sinica, 2009, 18(3): 59-66.

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