Effect of γ-irradiation on callus regeneration and salt tolerance in Manilagrass

Abstract

Abstract: Embryogenic calluses induced from stolons of Manilagrass (Zoysia matrelia) were treated with ⁶⁰Co γ-irradiation at doses of 0 Gy (control) and 10 Gy. Regeneration of the callus, activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) enzymes, and proline content in the regenerated plants were measured. The treated materials were screened on MS basal media with different concentrations of NaCl (0%, 0.5%, 1.0%, 1.5%, and 2.0%). The results showed that 10 Gy ⁶⁰Co γ-irradiation had a significant promoting effect on callus regeneration and differentiation, and that the percentage of explants that formed more than 3 plantlets per explant was increased by 11.25%. However, the regeneration rate of the callus was reduced by 8.75%. 10 Gy ⁶⁰Co γ-irradiation of regenerated plants resulted in increases of CAT activity and proline content by 16.02% and 1.20 times, respectively. There were no significant changes in the activities of SOD and POD enzymes in the regenerated plants from calluses treated with 10 Gy γ-irradiation, compared with that of the controls. The maximum number of regenerated plants were obtained from calluses treated with 10Gy ⁶⁰Co γ-irradiation and grown on medium with 1.0% NaCl - 9.60% of the calluses formed plantlets. Regenerated plants maintained vigorous growth after transfer to rooting medium with 1.0% NaCl.

CLC Number:

JIA Yu-fang, CHEN Shu, CHAI Ming-liang. Effect of γ-irradiation on callus regeneration and salt tolerance in Manilagrass[J]. Acta Prataculturae Sinica, 2010, 19(5): 25-30.

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