Analysis of differential expression of proteins in replanting disease of Pseudostellaria heterophylla

Abstract

Abstract: The differential proteomics method was used to analyze the differential expression of proteins in Pseudostellaria heterophylla leaves to reveal the molecular mechanism of P. heterophylla replanting disease in a P. heterophylla-Oryza sativa-P. heterophylla crop rotation or in P. heterophylla continuous cropping. There were 27 differential protein spots in the 2-D gelatin from the P. heterophylla leaves in both the crop rotation and the continuous cropping systems. Bioinformatics searches successfully identified 24 of the 27 protein spots. Senescence-associated similar proteins, the Ras-related protein, the senescence-related receptor-like kinases, the PR-proteins and the Ras-related protein RHN1 were all up-regulated. All of these proteins are related to plant senescence or disease. The phospholipid hydroperoxide glutathione peroxidase, plant-type serine-threonine protein kinase, calcium-dependent protein kinase, chalcone synthase, and thioredoxin-related proteins were all up-regulated, while the cell wall glycine-rich protein, the homologue to SKP1, the zinc finger protein, and the maturase K were all down-regulated. These proteins are related to plant resistance. Both the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and the cytochrome b₆ were down-regulated, and the ribulose bisphosphate carboxylase small chain c and the cytochrome b₆/f complex subunit VI were up-regulated. These proteins are related to photosynthesis. The sucrose-UDP glucosyltransferase and the NF-YB5 were down-regulated, and the proteasome subunit beta type 1, the synthase beta subunit, the cytokinin histidine phosphotransfer protein 4 and the NAD-dependent glyceraldehyde-3-phosphate dehydrogenase were all up-regulated. These proteins are related to plant metabolism of energy or cell division. It suggested that in P. heterophylla replanting disease, up-regulated expression of proteins associated with senescence or disease results in disturbed expressions of proteins related to plant resistance or to energy metabolism or cell division. The continuous planting of P. heterophylla resulted in down-regulated expression of the important photosynthesis related proteins, explaining the molecular basis for the declining photosynthesis rate in replanted P. heterophylla.

CLC Number:

LIN Mao-zi, ZHANG Zhi-xing, LIN Zheng-chun, YOU Chui-huai,
ZENG Ling-jie, LIN Wen-xiong. Analysis of differential expression of proteins in replanting disease of Pseudostellaria heterophylla[J]. Acta Prataculturae Sinica, 2010, 19(6): 197-207.

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