Phospholipid fatty acid analysis of microbial community structure under different land use patterns in soil ecosystems of Leymus chinensis steppes

Abstract

Abstract: Effects of different land use patterns on the structure of microbial communities of the Leymus chinensis steppe soil ecosystems were studied by phospholipid fatty acid analysis. Soil microbial community structure changed under different land use patterns. Microbial characterization of the total PLFA content of mown plots was significantly higher than those of the fenced and grazed plots, and total PLFA content of the fenced plots was the lowest. Correlation analysis showed that the total concentration of PLFA was significantly positively correlated with microbial biomass carbon as determined by the fumigation-extraction method. Mowing significantly increased both soil bacterial content and actinomycete content of fenced plots but soil fungal content was not significantly affected under different land use patterns. Bacterial characterization of PLFA in mown plots was the highest (29.6 nmol/g, bacterial contents) with grazed and fenced plots at 27.8% and 49.3% respectively. Dominant PLFA type of mown plots were a15:0, 15:0, 16:0 and 18:0, and accounted for 64.6% of total PLFA; Dominant PLFA type of grazed plots were i15:0, i16:0 and 18:1ω9t, and accounted for 45.6% of total PLFA; Dominant PLFA type of fenced plots were a15:0,15:0 and i16:0, and accounted for 46.3% of total PLFA.

CLC Number:

ZOU Yu-kun, ZHANG Jing-ni, YANG Dian-lin, CHEN Xiu-rong, ZHANG Tian-rui, ZHAO Jian-ning, ZHAO Shuai. Phospholipid fatty acid analysis of microbial community structure under different land use patterns in soil ecosystems of Leymus chinensis steppes[J]. Acta Prataculturae Sinica, 2011, 20(4): 27-33.

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