Analysis of physiological and biochemical characteristics of T1 salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway

Abstract

Abstract: With 300 mmol/L NaCl stress, the physiological and biochemical indexes, including chlorophyll, proline and MDA content, and antioxidase activity, of T₁ transgenic alfalfa transformed with Rhizophora apiculata total DNA by the pollen-tube pathway, were investigated. The transgenic plantlets had a reduced chlorophyll content of 0.09-0.29 times, but the non-transgenic plant had 0.32 times. The proline content of transgenic plantlets was enhanced by 1.2 to 4.2 times, but for non-transgenic plant it was 0.9 times. The increased proportion of MDA content was 0.15-0.29 times in transgenic plantlets compared with 0.36 times for non-transgenic plant. However, the transgenic plantlets had increased SOD activity (0.20-0.25 times) compared with the non-transgenic plant (0.19 times). The POD and CAT activity of the transgenic plantlets were 1.6-5.6 times and 1.2-1.3 times respectively compared with non- transgenic plants. This suggested that the transgenic plantlets had the physiological and biochemical characteristics of salt tolerant plants.

CLC Number:

ZHANG Li-quan, AO Deng-hua, SHI Wen-gui, NIU Yi-ding, HAN Lan, HASI Agula. Analysis of physiological and biochemical characteristics of T₁ salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway[J]. Acta Prataculturae Sinica, 2012, 21(2): 149-155.

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Analysis of physiological and biochemical characteristics of T₁ salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway

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