Cloning and analysis of the MADS-box gene WM8 of Elymus nutans

Abstract

Abstract: The full length MADS-box gene EnWM8 was isolated from Elymus nutans by homology cloning, and was submitted to GenBank (accession number JF683846). Analyses of the cDNA sequence showed that EnWM8 was 1 196 bp and encoded 275 amino acids, typical of the MIKC-type MADS-box genes with a typical MADS-box domain and K semi-conservative district. It is speculated that it has some connection with flower development, fruit ripening etc. The deduced amino acid sequence showed that there were 95.62%, 96.72%, 96.28% similarities between the gene and Triticum aestivum genes WM8, AGL29, and fruitful-like. The phylogenetic tree deduced by DNAMAN software indicated that EnWM8 had a close evolutionary relationship with the TaWM8 of T. aestivum, and was distantly related to the AtWM8 and AtAGL29 of Arabidopsis. The WM8 protein of E. nutans is a hydrophilic and basic protein with a molecular weight of 13 726.7 Da: the theoretical isoelectric point is 9.14. It has no membrane structure and is mainly located in the nucleus. There are many α-helix, random coil and extended strand structures. These results are a basis for further research on flower development, seed maturation and other mechanisms of E. nutans at the molecular level.

CLC Number:

ZHANG Miao-qing, ZHANG Ji-yu, LIU Zhi-peng, WANG Yan-rong, ZHANG Lei. Cloning and analysis of the MADS-box gene WM8 of Elymus nutans [J]. Acta Prataculturae Sinica, 2012, 21(4): 141-150.

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