Effects of SARA induced by high level concentrate on cortisol concentrations in serum and rumen fluids of the goats during lactation

Abstract

Abstract: Seven goats fitted with permanent rumen cannulas in mid-lactation were used in a 2×2 latin square experiment design with different concentrate to roughage ratio of 6∶4 and 4∶6 respectively. Set up the subacute ruminal acidosis (SARA) model by the diet with concentrate to roughage ratio of 6∶4 to study the variations of cortisol concentration in the blood and rumen fluid and glucocorticoid receptor mRNA of goat in mid-lactation. SARA was induced successfully by feeding the SARA group with the diet with concentrate to roughage ration of 6∶4. pH of the rumen fluid of SARA group was below 5.8 after feeding, lasting for about 6 hours while pH of the rumen fluid of control group (concentrate to roughage ratio of 4∶6) was above 6.0. pH and lactic acid of the rumen fluid, LPS and cortisol of rumen fluid and blood were affected by dietary modulation (P<0.05). Comparing with that in the control group, the concentration of lactic acid in rumen fluid of SARA group was significantly high (P<0.01) before feeding and then decreased in four hours after feeding and increased during 6-10 h after feeding, significantly higher (P<0.01). Concentration of LPS in rumen fluid was no significance between the two groups at the same time points (P>0.05), but concentration of LPS in blood of SARA group was significancantly higher than control in 0 and 6 h after feeding (P<0.05); the concentration of cortisol in blood and rumen fluid of SARA group was higher than that in the control group (P=0.05) before feeding, but there was no significance between the two groups after 6 hours after feeding (P>0.05); concentration of cortisol in rumen fluid was significantly higher than control in 2,4 and 6 h after feeding, but no significance between the two goups after 10 hours after feeding (P>0.05). Results of Real-time PCR showed that GR mRNA downgraded significantly (P<0.05). Our results indicates that SARA goats were in stress situation with a significantly higher glucocorticoid concentrations in serum and rumen fluids, accompanied with down-regulation of GR mRNA expression in liver which will change the status of metabolism and nutrition redistribution in liver.

CLC Number:

S827

JIA Yuan-yuan, WANG Shao-qing, CHANG Guang-jun, ZHANG Yuan-shu, ZHUANG Su, NI Ying-dong, SHEN Xiang-zhen, CHEN Jie, ZHAO Ru-qian. Effects of SARA induced by high level concentrate on cortisol concentrations in serum and rumen fluids of the goats during lactation[J]. Acta Prataculturae Sinica, 2012, 21(4): 259-266.

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