Phylogeny and function characterization of DREB transcription factors in Leymus chinensis

Abstract

Abstract: Leymus chinensis is an economically and ecologically important grass that is tolerant to salt, drought and cold stresses. DREB are important transcription factors in plant stress responses. However, there were only two genes of L. chinensis in the GenBank nucleotide and EST databases and only one of them had further study. The identification of 26 L. chinensis DREB ESTs were reported from analysis of transcriptome sequencing data. A phylogenetic tree was inferred from protein sequences of the 26 L. chinensis DREB transcription factors compared with all DREB transcription factors in the Arabidopsis thaliana genome. LcDREB21 belonged to group IV, and was a member of DREB2s. RACE was used to amplify the full-length CDS of LcDREB21 (GenBank accession number: JN860437). qRT-PCR results confirmed that LcDREB21 was induced by drought and high salinity stresses. Transcriptional activation of LcDREB21 was shown by yeast one-hybrid system, and nuclear localization of LcDREB21 was confirmed by expression of GFP fusion. In summary, LcDREB21 showed nuclear specific localization and transactivation activity, and may play roles in response to drought and high salinity stresses of L. chinensis. New members have been added to the family of stress-related genes in L. chinensis, and LcDREB21 could be used to improve stress tolerance in plants through genetic engineering.

CLC Number:

MA Xing-yong, PENG Xian-jun, SU Man, ZHANG Le-xin, ZHOU Qing-yuan, CHEN Shuang-yan, CHENG Li-qin, LIU Gong-she. Phylogeny and function characterization of DREB transcription factors in Leymus chinensis[J]. Acta Prataculturae Sinica, 2012, 21(6): 190-197.

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