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Acta Prataculturae Sinica ›› 2013, Vol. 22 ›› Issue (1): 260-267.

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Genetic diversity analysis of Agropyron germplasm resources by ISSR

ZENG Liang1,2, YUAN Qing-hua1, WANG Fang1,3, WANG Yu1   

  1. 1.Institute of Animal Science, CAAS, Beijing 100193, China;
    2.College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China;
    3.College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2012-01-13 Online:2013-01-25 Published:2013-02-20

Abstract: The genetic diversity of 33 Agropyron materials from both at home and abroad was analyzed by ISSR (inter-simple sequence repeats) markers. Eleven primers were selected from 93 primers which had clear polymorphic bands. A total of 84 bands of which 59 were polymorphic were obtained from the 33 materials. On average, each primer amplification site was 7.64. The genetic similarity coefficient of varieties was between 0.083 and 0.706, and showed rich genetic diversity. UPGMA cluster analysis gave a genetic similarity coefficient of 0.52 for boundaries and the 33 materials were divided into four types, with basic clustering in accord with geographical origin and regional distribution.

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