Effects of endophytic Bacillus subtilis 265ZY4 on physiological and biochemical characteristics of Stipa purpurea under abiotic stress

doi:10.11686/cyxb2018342

Abstract

Abstract: In order to understand the effects of the endophytic bacterium, Bacillus subtilis, on Stipa purpurea under high temperature, low temperature and ultraviolet stress, the biochemical characteristics of S. purpurea inoculated with B. subtilis strain 265ZY4 were compared with those of plants not carrying the bacterial endophyte. The results showed that the height and leaf width of S. purpurea with 265ZY4 were 35.02% and 34.69%, respectively, more than in the control plants. When S. purpurea was subject to high- or low-temperature stress, leaf chlorophyll content rose and then fell. Under ultraviolet stress, chlorophyll levels dropped rapidly, but the chlorophyll content of S. purpurea with 265ZY4 remained higher than those in the control. The soluble protein and soluble sugar levels also initially rose and then dropped under abiotic stress, but the soluble sugar levels of S. purpurea with 265ZY4 were distinctly higher than those in the control plants after 4 and 6 days. The superoxide dismutase (SOD) activity displayed a decrease-increase-decrease curve with progressive increase in high- and low-temperature stress, but the SOD activity of S. purpurea with 265ZY4 was more stable. The SOD activity of the control plants showed a decrease-increase-decrease curve pattern under increasing ultraviolet exposure, but the activity of S. purpurea with 265ZY4 showed a curve pattern that differed from the control plants. The phenylalnine ammonialyase (PAL) activity displayed an increase-decrease curve pattern under low temperature stress and a decrease-increase-decrease curve under high temperature and ultraviolet stress, but the PAL activities of S. purpurea plants with 265ZY4 were distinctly higher than those in the control. In summary, the results show that the endophytic B. subtilis strain 265ZY4 is beneficial to S. pupurea through assisting with maintenance of chlorophyll, soluble protein, and soluble sugar levels, and SOD and PAL activities, and reducing the range of metabolic variability under the abiotic stress factors tested, thus indicating enhancement of S. purpurea stress tolerance ability.

Key words: endophytic bacteria, Stipa purpurea, stress, physiological and biochemical characteristics

YANG Cheng-de, CUI Yue-zhen, FENG Zhong-hong, XUE Li, JIN Meng-jun. Effects of endophytic Bacillus subtilis 265ZY4 on physiological and biochemical characteristics of Stipa purpurea under abiotic stress[J]. Acta Prataculturae Sinica, 2019, 28(6): 101-108.

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