Damage to the AsA-GSH cycle of soybean leaves under waterlogging stress at in seed filling period growth stages and the mitigation effect of uniconazole

doi:10.11686/cyxb2020249

Abstract

Abstract:

This research explored the effect of spraying uniconazole （S3307） on two different soybean varieties under waterlogging stress. The waterlogging-tolerant variety Kenfeng 14 and the waterlogging-sensitive variety Kenfeng 16 were studied in pot experiments. The degree of membrane lipid peroxidation as indicated by malondialdehyde （MDA） concentration， and levels of reactive oxygen species （ROS） and peptide ascorbic acid-glutathione （AsA-GSH） in soybean leaves under waterlogging stress at growth stage R5 （seed filling period） were evaluated. AsA-GSH circulatory system damage was detected and S3307 had an alleviating effect. The results showed that waterlogging stress at the R5 growth stage significantly increased the MDA content in the leaves of the two tested soybean varieties and accelerated the accumulation of ROS. A greater increase was observed in the waterlogging-sensitive variety Kenfeng 16 than in Kenfeng 14. After foliar spraying with S3307， the concentration of non-enzyme antioxidants and the activities of key enzymes were increased， and the MDA concentration of leaves was reduced. S3307 inhibited the accumulation of ROS， reduced the damage caused by waterlogging stress to the membrane system， and assisted the maintenance of a high level of key enzyme activity and non-enzymatic antioxidant concentrations. The benefits of S3307 application persisted after plants were returned to normal soil water levels， S3307 and promoted the recovery of the two soybean varieties to a normal growth status. The recovery ability of waterlogging-tolerant variety Kenfeng 14 was better than Kenfeng 16. In summary， waterlogging stress had contrast effects on the AsA-GSH cycle in the leaves of two soybean varieties with different waterlogging tolerances. S3307 partially alleviated the damage caused by the waterlogging stress.

Key words: soybean, waterlogging stress, leaf, uniconazole, AsA-GSH cycle

Shi-ya WANG, Dian-feng ZHENG, Nai-jie FENG, Xi-long LIANG, Hong-tao XIANG, Sheng-jie FENG, Xin-xin WANG, Guan-qiang ZUO. Damage to the AsA-GSH cycle of soybean leaves under waterlogging stress at in seed filling period growth stages and the mitigation effect of uniconazole[J]. Acta Prataculturae Sinica, 2021, 30(7): 157-166.

Figures/Tables 9

Table 1 Experiment design

品种Varieties	处理编号Treatment code	药剂处理Pesticide treatment	水分处理Water treatment
垦丰14 Kenfeng 14	T₁	清水喷施 Spray water	正常水分 Normal water
	T₂	清水喷施 Spray water	淹水胁迫 Waterlogging stress
	T₃	S3307喷施 Spray S3307	淹水胁迫 Waterlogging stress
垦丰16 Kenfeng 16	T₄	清水喷施 Spray water	正常水分 Normal water
	T₅	清水喷施 Spray water	淹水胁迫 Waterlogging stress
	T₆	S3307喷施 Spray S3307	淹水胁迫 Waterlogging stress

Fig.1 Effects of S3307 on MDA content in leaves of soybean under waterlogging stress at R5 stage

Fig.2 Effects of S3307 on O2—· production rate and H2O2 content in leaves of soybean under waterlogging stress at R5 stage

Fig.3 Effects of S3307 on AsA and DHA and AsA+DHA content in leaves of soybean under waterlogging stress at R5 stage

Fig.4 Effects of S3307 on GSH and GSSG and GSH+GSSG content in leaves of soybean under waterlogging stress at R5 stage

Fig.5 Effects of S3307 on APX activity in leaves of soybean under waterlogging stress at R5 stage

Fig.6 Effects of S3307 on GR activity in leaves of soybean under waterlogging stress at R5 stage

Fig.7 Effects of S3307 on MDHAR activity in leaves of soybean under waterlogging stress at R5 stage

Fig.8 Effects of S3307 on DHAR activity in leaves of soybean under waterlogging stress at R5 stage

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