Trichoderma asperellum bai5 inhibits root rot pathogens of alfalfa （Medicago sativa） and promotes alfalfa plant growth

doi:10.11686/cyxb2024365

Abstract

Abstract:

The aim of this work was to screen for fungal biocontrol strains with inhibitory effects on the pathogens causing root rot of alfalfa （Medicago sativa）. To this end， the fungal strain bai5 was isolated and obtained from the soil surrounding the roots of Betula platyphylla by the dilution isolation method. This strain was identified as Trichoderma asperellum through morphological and molecular biological identification methods. Seven common root rot pathogens of alfalfa （Fusarium avenaceum FAV-7， Fusarium equiseti FEQ-10， Fusarium acuminatum FAC-11， Fusarium chlamydosporum FCH-13， Fusarium tricinctum FTR-14， Fusarium oxysporum FOX-15， Fusarium solani FSO-16） were used as target pathogens， and the inhibitory effect of strain bai5 on these pathogens was comprehensively evaluated using the two-point standoff method， the solid dilution method， and the plate-pair buckling method. The inhibitory rate of strain bai5 against the seven fungal pathogens was higher than 69% in the standoff culture test. In the fermentation filtrate inhibition test， strain bai5 inhibited the growth of FAC-11， FTR-14， FEQ-10， FCH-13， FAV-7， and FSO-16 by 63.14%， 49.80%， 40.78%， 33.33%， 16.08%， and 8.20%， respectively， but it had no inhibitory effect on FOX-15. The volatile compounds of strain bai5 inhibited the growth of FTR-14 and FAC-11 by up to 74.51% and 72.00%， respectively. Analyses of the biological properties and growth-promoting characteristics of strain bai5 showed that it grows fast， is strongly stress resistant， and has nitrogen-fixation and cellulase-producing abilities. The effect of T. asperellum bai5 on the growth and physiological indexes of pot-grown alfalfa plants was determined. The results show that bai5 significantly increased the fresh weight， height of above-ground parts， dry weight， chlorophyll content， and soluble sugars content of alfalfa plants. In conclusion， T. asperellum bai5 can inhibit the growth of at least seven root rot fungi of alfalfa and significantly promote alfalfa plant growth. Therefore， it is a biocontrol fungus with high development potential.

Key words: Trichoderma asperellum, separation and identification, alfalfa root rot disease, Fusarium, antibacterial effect, promote growth

Hua-ying DU, Yu-zhou ZHANG, Nan ZHAO, Yan HU, Yi-dong WANG, Teng-da LIU, Pei-wen GU, Ze-yang YU. Trichoderma asperellum bai5 inhibits root rot pathogens of alfalfa （Medicago sativa） and promotes alfalfa plant growth[J]. Acta Prataculturae Sinica, 2025, 34(8): 179-190.

Figures/Tables 10

Fig.1 Morphological characteristics of bai5 strain on PDA medium

Fig.2 Phylogenetic tree of strain bai5 and related relatives based on the joint construction of ITS and tef 1-α sequences

Fig.3 Inhibition effect of the mycelium of T. asperellum bai5 on seven species of Fusarium spp.

Fig.4 Inhibitory effect of sterile fermentation filtrate of T. asperellum bai5 on seven species of Fusarium spp.

Fig.5 Inhibitory effect of volatiles of T. asperellum bai5 on seven species of Fusarium spp.

Fig. 6 Inhibitory effect of T. asperellum bai5

Fig.7 Growth rate and resistance properties of T. asperellum bai5

Fig.8 Growth-promoting function of T. asperellum bai5 on alfalfa

Fig.9 Growth-promoting effect of T. asperellum bai5 on alfalfa

Table 1 Growth-promoting effect of T. asperellum bai5 on alfalfa

棘孢木霉bai5

T. asperellum bai5

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