Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (4): 113-123.DOI: 10.11686/cyxb2021323
Chen-qin LI1,2,3(), Jun-qiao LI1,2,3(), Xin-ci WANG1,2,3, Yong-kun NIU1,2,3, Jun-ru QU1,2,3
Received:
2021-08-30
Revised:
2021-10-18
Online:
2022-04-20
Published:
2022-01-25
Contact:
Jun-qiao LI
Chen-qin LI, Jun-qiao LI, Xin-ci WANG, Yong-kun NIU, Jun-ru QU. Isolation, identification, and biological characteristics of Fusarium perseae isolated from Potentilla anserina roots[J]. Acta Prataculturae Sinica, 2022, 31(4): 113-123.
处理 Treatment | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
缺碳对照 No carbon source (C1) | 4.67±0.07Aa | 16.67±1.67Aa |
葡萄糖 Glucose (C2) | 5.43±0.02Cd | 93.33±4.41Bb |
蔗糖Sucrose (C3) | 5.18±0.07BCc | 223.33±7.26Ee |
乳糖Lactose (C4) | 4.65±0.08Aa | 178.33±8.82Dd |
可溶性淀粉Soluble starch (C5) | 5.37±0.07Ccd | 236.67±7.26Ee |
果糖Fructose (C6) | 4.98±0.07Bb | 263.33±7.26Ff |
甘露醇Mannitol (C7) | 5.28±0.02Ccd | 141.67±1.67Cc |
Table 1 Effects of different carbon source on mycelium growth and sporulation
处理 Treatment | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
缺碳对照 No carbon source (C1) | 4.67±0.07Aa | 16.67±1.67Aa |
葡萄糖 Glucose (C2) | 5.43±0.02Cd | 93.33±4.41Bb |
蔗糖Sucrose (C3) | 5.18±0.07BCc | 223.33±7.26Ee |
乳糖Lactose (C4) | 4.65±0.08Aa | 178.33±8.82Dd |
可溶性淀粉Soluble starch (C5) | 5.37±0.07Ccd | 236.67±7.26Ee |
果糖Fructose (C6) | 4.98±0.07Bb | 263.33±7.26Ff |
甘露醇Mannitol (C7) | 5.28±0.02Ccd | 141.67±1.67Cc |
处理 Treatment | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
缺氮对照 No nitrogen source (N1) | 4.22±0.04Dd | 153.33±4.41Bb |
甘氨酸Glycine (N2) | 4.47±0.04Ee | 241.67±6.67Dd |
磷酸二氢铵Ammonium dihydrogen phosphate (N3) | 2.45±0.03Aa | 280.00±7.64Ee |
硝酸铵Ammonium nitrate (N4) | 3.17±0.03Bb | 186.67±3.33Cc |
硫酸铵Ammonium sulphate (N5) | 3.27±0.03Bb | 320.00±8.66Ff |
蛋白胨Peptone (N6) | 3.53±0.02Cc | 90.00±0.00Aa |
牛肉浸膏Beef extract (N7) | 4.27±0.02Dd | 285.00±7.64Ee |
硝酸钠Sodium nitrate (N8) | 5.18±0.07Ff | 223.33±7.26Dd |
Table 2 Effects of different nitrogen source on mycelium growth and sporulation
处理 Treatment | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
缺氮对照 No nitrogen source (N1) | 4.22±0.04Dd | 153.33±4.41Bb |
甘氨酸Glycine (N2) | 4.47±0.04Ee | 241.67±6.67Dd |
磷酸二氢铵Ammonium dihydrogen phosphate (N3) | 2.45±0.03Aa | 280.00±7.64Ee |
硝酸铵Ammonium nitrate (N4) | 3.17±0.03Bb | 186.67±3.33Cc |
硫酸铵Ammonium sulphate (N5) | 3.27±0.03Bb | 320.00±8.66Ff |
蛋白胨Peptone (N6) | 3.53±0.02Cc | 90.00±0.00Aa |
牛肉浸膏Beef extract (N7) | 4.27±0.02Dd | 285.00±7.64Ee |
硝酸钠Sodium nitrate (N8) | 5.18±0.07Ff | 223.33±7.26Dd |
培养基 Medium | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
蕨麻煎汁培养基 P. anserina juice medium (JM) | 4.35±0.08Aa | 251.67±3.33BCc |
硝酸钠为氮源的蕨麻煎汁培养基 P. anserina juice medium with sodium nitrate (JMN) | 4.33±0.04Aa | 261.67±10.14Cc |
葡萄糖为碳源的蕨麻煎汁培养基 P. anserina juice medium with glucose (JMC) | 5.18±0.09Bb | 353.33±1.67Dd |
Luria-Bertani培养基Lysogeny broth medium (LB) | 4.50±0.00Aa | 155.00±10.41Aa |
马铃薯葡萄糖琼脂培养基 Potato dextrose agar medium (PDA) | 5.28±0.03Bb | 351.67±7.26Dd |
查贝克氏培养基 Czapek’s medium (CZ) | 5.18±0.07Bb | 223.33±7.26Bb |
Table 3 Effects of medium on mycelium growth and sporulation
培养基 Medium | 菌落直径 Colony diameter (cm) | 产孢量 Sporulation (×105·mL-1) |
---|---|---|
蕨麻煎汁培养基 P. anserina juice medium (JM) | 4.35±0.08Aa | 251.67±3.33BCc |
硝酸钠为氮源的蕨麻煎汁培养基 P. anserina juice medium with sodium nitrate (JMN) | 4.33±0.04Aa | 261.67±10.14Cc |
葡萄糖为碳源的蕨麻煎汁培养基 P. anserina juice medium with glucose (JMC) | 5.18±0.09Bb | 353.33±1.67Dd |
Luria-Bertani培养基Lysogeny broth medium (LB) | 4.50±0.00Aa | 155.00±10.41Aa |
马铃薯葡萄糖琼脂培养基 Potato dextrose agar medium (PDA) | 5.28±0.03Bb | 351.67±7.26Dd |
查贝克氏培养基 Czapek’s medium (CZ) | 5.18±0.07Bb | 223.33±7.26Bb |
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