Anastomosis groups and pathogenicity differentiation of the rice sheath blight pathogen in China

doi:10.11686/cyxb2017316

Abstract

Abstract: In order to confirm the anastomosis groups of rice sheath blight and their pathogenicity in rice-growing areas of China, samples of rice sheath blight were collected from Heilongjiang, Jilin, Liaoning, Henan, Anhui, Zhejiang, Jiangsu, Sichuan, Chongqing, Guizhou, Yunnan, Hubei, Hunan, Guangxi, Guangdong and Fujian in 2015. The pathogens were isolated from the samples using the water agar separation method. The isolated pathogens were then screened and identified according to morphological characteristics, nucleus fluorescence staining and rDNA-ITS sequence analysis. The growth rate, sclerotium structure and number of cell nuclei of pathogen isolates from different regions were compared. The pathogenicity of these pure cultured strains from the infected rice tissues was tested according to Koch’s law. The sixty-one isolates had loose mycelium and were identified as Rhizoctonia solani AG1IA. Mycelia were much branched and diaphragmatic and were multinucleate. Branches were at right angles or close to 45°. Pathogen isolates from different regions displayed different growth rates, sclerotia structures, and numbers of cell nuclei. Pathogenicity tests showed that 16 of the 61 strains could cause symptoms of rice sheath blight. The pathogenicity of isolates from Guangdong, Guangxi, Fujian and Chongqing was much higher than the pathogenicity of other strains.

Key words: rice, Rhizoctonia solani, anastomosis groups, pathogenicity

WANG Ai-jun, WANG Na, GU Si-si, ZHAO Wen-juan, LI Ping, ZHENG Ai-ping. Anastomosis groups and pathogenicity differentiation of the rice sheath blight pathogen in China[J]. Acta Prataculturae Sinica, 2018, 27(7): 55-63.

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