Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (3): 158-166.DOI: 10.11686/cyxb2020147
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Di ZHANG1,2(), Li-fei REN1, Guang-bin LIU3, Fu-qing LUO4, Wen-hao ZHANG1, Tian-zuo WANG1()
Received:
2020-03-31
Revised:
2020-06-04
Online:
2021-03-20
Published:
2021-03-09
Contact:
Tian-zuo WANG
Di ZHANG, Li-fei REN, Guang-bin LIU, Fu-qing LUO, Wen-hao ZHANG, Tian-zuo WANG. Comparative metabolite profiling of alfalfa seeds dried at different temperatures[J]. Acta Prataculturae Sinica, 2021, 30(3): 158-166.
编号Codes | 比较组Comparison groups |
---|---|
A | 自然干燥/新鲜种子Natural drying/fresh seeds |
B | 39 ℃干燥/新鲜种子 39 ℃ drying/fresh seeds |
C | 65 ℃干燥/新鲜种子 65 ℃ drying/fresh seeds |
D | 39 ℃干燥/自然干燥 39 ℃ drying/natural drying |
E | 65 ℃干燥/自然干燥 65 ℃ drying/natural drying |
F | 65 ℃干燥/39 ℃干燥 65 ℃ drying/39 ℃ drying |
Table 1 Comparison groups
编号Codes | 比较组Comparison groups |
---|---|
A | 自然干燥/新鲜种子Natural drying/fresh seeds |
B | 39 ℃干燥/新鲜种子 39 ℃ drying/fresh seeds |
C | 65 ℃干燥/新鲜种子 65 ℃ drying/fresh seeds |
D | 39 ℃干燥/自然干燥 39 ℃ drying/natural drying |
E | 65 ℃干燥/自然干燥 65 ℃ drying/natural drying |
F | 65 ℃干燥/39 ℃干燥 65 ℃ drying/39 ℃ drying |
分类Groups | 数目Number |
---|---|
脂类及类脂分子Lipids and lipid-like molecules | 188 |
有机酸及其衍生物Organic acids and derivatives | 106 |
苯丙酸和聚酮Phenylpropanoids and polyketides | 72 |
未分类Unclassed | 59 |
有机杂环化合物Organoheterocyclic compounds | 33 |
有机氧化合物Organic oxygen compounds | 28 |
苯环型化合物Benzenoids | 23 |
核苷、核苷酸及类似物Nucleosides, nucleotides, and analogues | 23 |
有机氮化合物Organic nitrogen compounds | 7 |
生物碱及其衍生物Alkaloids and derivatives | 5 |
磺胺嘧啶Sulfadiazine | 1 |
Table 2 Classification of identified metabolites
分类Groups | 数目Number |
---|---|
脂类及类脂分子Lipids and lipid-like molecules | 188 |
有机酸及其衍生物Organic acids and derivatives | 106 |
苯丙酸和聚酮Phenylpropanoids and polyketides | 72 |
未分类Unclassed | 59 |
有机杂环化合物Organoheterocyclic compounds | 33 |
有机氧化合物Organic oxygen compounds | 28 |
苯环型化合物Benzenoids | 23 |
核苷、核苷酸及类似物Nucleosides, nucleotides, and analogues | 23 |
有机氮化合物Organic nitrogen compounds | 7 |
生物碱及其衍生物Alkaloids and derivatives | 5 |
磺胺嘧啶Sulfadiazine | 1 |
比较组 Comparison groups | 代谢物 Metabolites | 含量变化 Content change | 分类 Groups |
---|---|---|---|
A、B和C三个比较组共有差异代谢物 The common differential metabolites among A, B and C comparison groups | 8-羟基鸟嘌呤8-Hydroxyguanine | 下调Down-regulation | 有机杂环化合物Organoheterocyclic compounds |
15-羟基二十碳-5Z,8Z,11Z,13E-四烯酸 5-Hydroperoxy-5Z,8Z,11Z,13E-Eicosatetraenoic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
槲皮素Quercetin | 下调Down-regulation | 苯丙酸和聚酮 Phenylpropanoids and polyketides | |
C、E和F三个比较组共有差异代谢物 The common differential metabolites among C, E and F comparison groups | 1,3,5-苯三酚1,3,5-Benzenetriol | 上调Up-regulation | 有机杂环化合物Organoheterocyclic compounds |
脱氧腺苷 Deoxyadenosine | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3'-磷酸腺苷3'-Adenosine monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2',3'-环磷酸腺苷 Adenosine 2',3'-cyclic phosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2'-磷酸腺苷Adenosine 2'-Monophosphate | 上调Up-regulation | 有机氧化合物 Organic oxygen compounds | |
腺苷Adenosine | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
磷酸腺苷Adenosine phosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2',3'-环磷酸鸟苷 Guanosine-2',3'-cyclic monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
戊草丹Esprocarb | 上调Up-regulation | 未分类Unclassed | |
鸟苷Guanosine | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
磷酸鸟苷 Guanosine monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3'-磷酸鸟苷Guanosine 3'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3',5'-环磷酸鸟苷 Guanosine-3',5'-Cyclic monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
异亮氨酸-脯氨酸Ile-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
苯丙氨酸-脯氨酸Phe-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
尿苷5'-单磷酸Uridine 5'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
缬氨酸-脯氨酸Val-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
B和D两个比较组共有差异代谢物The common differential metabolites between B and D comparison groups | L-谷胱甘肽(氧化型)L-Glutathione (oxidized form) | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives |
C和E两个比较组共有差异代谢物 The common differential metabolites between C and E comparison groups | 3-恶辛酸3-Oxooctadecanoic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules |
13-羟基亚油酸13-Hydroxylinoleic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
溶血磷脂酰乙醇胺 LysoPE 18:2 | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
C和F两个比较组共有差异代谢物 The common differential metabolites between C and F comparison groups | 5'-磷酸胞苷 Cytidine-5'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues |
5'-磷酸胸腺嘧啶 Thymidine-5'-Monophosphate | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
E和F两个比较组共有差异代谢物 The common differential metabolites between E and F comparison groups | 3'-磷酸胞苷 Cytidine-3'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物Nucleosides, nucleotides, and analogues |
鸟嘌呤Guanine | 上调Up-regulation | 有机杂环化合物Organoheterocyclic compounds | |
A比较组特有差异代谢物 The specific differential metabolite in A comparison group | 儿茶素Catechin | 下调Down-regulation | 苯丙酸和聚酮 Phenylpropanoids and polyketides |
C比较组特有差异代谢物 The specific differential metabolites in C comparison group | 桦木酸Betulinic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules |
虫草素Cordycepin | 下调Down-regulation | 未分类Unclassed | |
3-羟基十二酸乙酯Ethyl 3-Hydroxydodecanoate | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
苏氨酸-γ-谷氨酸盐Threoninyl-Gamma-Glutamate | 下调Down-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
反式玉米素核糖 Trans-Zeatin riboside | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues |
Table 3 The common and specific differential metabolites among comparison groups
比较组 Comparison groups | 代谢物 Metabolites | 含量变化 Content change | 分类 Groups |
---|---|---|---|
A、B和C三个比较组共有差异代谢物 The common differential metabolites among A, B and C comparison groups | 8-羟基鸟嘌呤8-Hydroxyguanine | 下调Down-regulation | 有机杂环化合物Organoheterocyclic compounds |
15-羟基二十碳-5Z,8Z,11Z,13E-四烯酸 5-Hydroperoxy-5Z,8Z,11Z,13E-Eicosatetraenoic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
槲皮素Quercetin | 下调Down-regulation | 苯丙酸和聚酮 Phenylpropanoids and polyketides | |
C、E和F三个比较组共有差异代谢物 The common differential metabolites among C, E and F comparison groups | 1,3,5-苯三酚1,3,5-Benzenetriol | 上调Up-regulation | 有机杂环化合物Organoheterocyclic compounds |
脱氧腺苷 Deoxyadenosine | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3'-磷酸腺苷3'-Adenosine monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2',3'-环磷酸腺苷 Adenosine 2',3'-cyclic phosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2'-磷酸腺苷Adenosine 2'-Monophosphate | 上调Up-regulation | 有机氧化合物 Organic oxygen compounds | |
腺苷Adenosine | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
磷酸腺苷Adenosine phosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
2',3'-环磷酸鸟苷 Guanosine-2',3'-cyclic monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
戊草丹Esprocarb | 上调Up-regulation | 未分类Unclassed | |
鸟苷Guanosine | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
磷酸鸟苷 Guanosine monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3'-磷酸鸟苷Guanosine 3'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
3',5'-环磷酸鸟苷 Guanosine-3',5'-Cyclic monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
异亮氨酸-脯氨酸Ile-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
苯丙氨酸-脯氨酸Phe-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
尿苷5'-单磷酸Uridine 5'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
缬氨酸-脯氨酸Val-Pro | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
B和D两个比较组共有差异代谢物The common differential metabolites between B and D comparison groups | L-谷胱甘肽(氧化型)L-Glutathione (oxidized form) | 上调Up-regulation | 有机酸及其衍生物 Organic acids and derivatives |
C和E两个比较组共有差异代谢物 The common differential metabolites between C and E comparison groups | 3-恶辛酸3-Oxooctadecanoic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules |
13-羟基亚油酸13-Hydroxylinoleic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
溶血磷脂酰乙醇胺 LysoPE 18:2 | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
C和F两个比较组共有差异代谢物 The common differential metabolites between C and F comparison groups | 5'-磷酸胞苷 Cytidine-5'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues |
5'-磷酸胸腺嘧啶 Thymidine-5'-Monophosphate | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues | |
E和F两个比较组共有差异代谢物 The common differential metabolites between E and F comparison groups | 3'-磷酸胞苷 Cytidine-3'-Monophosphate | 上调Up-regulation | 核苷、核苷酸及类似物Nucleosides, nucleotides, and analogues |
鸟嘌呤Guanine | 上调Up-regulation | 有机杂环化合物Organoheterocyclic compounds | |
A比较组特有差异代谢物 The specific differential metabolite in A comparison group | 儿茶素Catechin | 下调Down-regulation | 苯丙酸和聚酮 Phenylpropanoids and polyketides |
C比较组特有差异代谢物 The specific differential metabolites in C comparison group | 桦木酸Betulinic acid | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules |
虫草素Cordycepin | 下调Down-regulation | 未分类Unclassed | |
3-羟基十二酸乙酯Ethyl 3-Hydroxydodecanoate | 下调Down-regulation | 脂类及类脂分子 Lipids and lipid-like molecules | |
苏氨酸-γ-谷氨酸盐Threoninyl-Gamma-Glutamate | 下调Down-regulation | 有机酸及其衍生物 Organic acids and derivatives | |
反式玉米素核糖 Trans-Zeatin riboside | 下调Down-regulation | 核苷、核苷酸及类似物 Nucleosides, nucleotides, and analogues |
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