Screening for plant growth-promoting rhizobacteria that promote cadmium absorption and accumulation and their effects on annual ryegrass

doi:10.11686/cyxb2020252

Abstract

Abstract:

Some plant growth-promoting rhizobacteria （PGPR） have the ability to promote plant growth and increase heavy metal absorption. Therefore， there is an opportunity to improve the efficiency of phytoremediation by inoculating the rhizosphere of plants which accumulate a large biomass with relevant strains of bacteria. In this study， 30 cadmium （Cd）-tolerant PGPR strains were isolated from soil of the plant rhizosphere in cadmium-contaminated areas. Based on the results of indole-3-acetic acid secretion， phosphorus dissolution， 1-aminocyclopropane-1-carboxylate deaminase production， iron carrier and growth promotion experiments， two PGPR strains designated Pi01 and Ma02 were selected and inoculated onto two cultivars of annual ryegrass with contrasting cadmium tolerance， IdyⅡ and Wasehope， and the effects on the growth and cadmium accumulation of ryegrass were measured. A pot culture test was carried out using soil contaminated with Cd at a concentration of （19.5±0.42） mg·kg^-1， and the two PGPR strains， Pi01 and Ma02， were inoculated onto the two cultivars of annual ryegrass. The biomass and Cd accumulation of both ryegrasses were significantly increased by inoculation with Pi01 and Ma02. In addition， inoculation with PGPR increased chlorophyll content， photosynthetic parameters， and extractable Cd concentration in the rhizosphere. Therefore， the two selected PGPRs， Pi01 and Ma02， can promote the growth of annual ryegrass and increase its accumulation of cadmium.

Key words: plant growth-promoting rhizobacteria, ryegrass, cadmium, cadmium accumulation

Hui-li WU, Wei TIAN, Yan-ling JI, Lai-qing LOU, Qing-sheng CAI. Screening for plant growth-promoting rhizobacteria that promote cadmium absorption and accumulation and their effects on annual ryegrass[J]. Acta Prataculturae Sinica, 2021, 30(7): 53-61.

Figures/Tables 9

References 34

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菌株 Strains	IAA^a Secreting IAA ability	溶磷^b Phosphate solubilization	ACC脱氨酶 ACC deaminase activity	铁载体 Producing siderophores	菌株 Strains	IAA^a Secreting IAA ability	溶磷^b Phosphate solubilization	ACC脱氨酶 ACC deaminase activity	铁载体 Producing siderophores
Ts01	-	-	-	+	Cc08	-	-	+	-
Tm01	-	++++	-	-	Pi06	++	-	-	-
As12	-	-	-	-	Pi11	++	-	-	-
Ls01	+	-	-	-	Pl01	++	-	-	+
Ma04	++	-	-	-	Ki02	-	-	-	+
Pi01	++	-	-	+	Mh02	+++	+	-	-
Ma02	++	++	-	+	Fs01	-	-	-	-
Pi03	+++	-	-	+	Af05	-	+	+	-
Sp01	-	+	-	+	As05	++	++++	-	-
Cch02	-	-	-	+	Ts02	-	-	-	-
Fs03	-	+++	-	-	Ra07	-	-	-	+
Ma11	-	-	-	+	As16	-	+++	-	-
Ls03	-	+	+	-	Pv02	-	++	-	-
As13	++	-	-	-	Cc09	-	+	-	-
Ra12	-	-	-	+	Cc11	-	+	-	-

菌株 Strains	IAA^a Secreting IAA ability	溶磷^b Phosphate solubilization	ACC脱氨酶 ACC deaminase activity	铁载体 Producing siderophores	菌株 Strains	IAA^a Secreting IAA ability	溶磷^b Phosphate solubilization	ACC脱氨酶 ACC deaminase activity	铁载体 Producing siderophores
Ts01	-	-	-	+	Cc08	-	-	+	-
Tm01	-	++++	-	-	Pi06	++	-	-	-
As12	-	-	-	-	Pi11	++	-	-	-
Ls01	+	-	-	-	Pl01	++	-	-	+
Ma04	++	-	-	-	Ki02	-	-	-	+
Pi01	++	-	-	+	Mh02	+++	+	-	-
Ma02	++	++	-	+	Fs01	-	-	-	-
Pi03	+++	-	-	+	Af05	-	+	+	-
Sp01	-	+	-	+	As05	++	++++	-	-
Cch02	-	-	-	+	Ts02	-	-	-	-
Fs03	-	+++	-	-	Ra07	-	-	-	+
Ma11	-	-	-	+	As16	-	+++	-	-
Ls03	-	+	+	-	Pv02	-	++	-	-
As13	++	-	-	-	Cc09	-	+	-	-
Ra12	-	-	-	+	Cc11	-	+	-	-

菌株Strains	革兰氏染色Gram stain	菌株形态特征Morphological characteristics of strains
菌株Strains	革兰氏染色Gram stain	形态Shape	表面Surface	边缘Edge	颜色Color	透明度Transparency	隆起状况Uplift
Ma02	-	杆状Rod-shaped	湿润Wet	整齐Neat	淡黄色Light yellow	不透明Opaque	凸起Raised
Pi01	-	杆状Rod-shaped	湿润Wet	整齐Neat	淡黄色Light yellow	不透明Opaque	凸起Raised

菌株Strains	革兰氏染色Gram stain	菌株形态特征Morphological characteristics of strains
菌株Strains	革兰氏染色Gram stain	形态Shape	表面Surface	边缘Edge	颜色Color	透明度Transparency	隆起状况Uplift
Ma02	-	杆状Rod-shaped	湿润Wet	整齐Neat	淡黄色Light yellow	不透明Opaque	凸起Raised
Pi01	-	杆状Rod-shaped	湿润Wet	整齐Neat	淡黄色Light yellow	不透明Opaque	凸起Raised

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