Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (7): 64-75.DOI: 10.11686/cyxb2021197
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Jian-zhen GE(), Wen-hui FU, Lu ZHANG, Bao-jun LIN, Shuai ZHAO, Ma-ga-weng BAI, Jian-cun KOU()
Received:
2021-05-11
Revised:
2021-08-20
Online:
2022-07-20
Published:
2022-06-01
Contact:
Jian-cun KOU
Jian-zhen GE, Wen-hui FU, Lu ZHANG, Bao-jun LIN, Shuai ZHAO, Ma-ga-weng BAI, Jian-cun KOU. Degradation of carbendazim in orchard white clover silage and its effect on the microbial fermentative community[J]. Acta Prataculturae Sinica, 2022, 31(7): 64-75.
项目Item | 空白对照 CON | RU- | RU | RU+ | 标准误SEM | P-value |
---|---|---|---|---|---|---|
乳酸Lactic acid (mg·mL-1) | 2.04±0.09c | 3.01±0.20b | 3.33±0.11a | 3.49±0.10a | 0.172 | 0.017 |
乙酸Acetic acid (mg·mL-1) | 0.49±0.05c | 0.61±0.04b | 0.71±0.06a | 0.72±0.04a | 0.738 | 0.030 |
丙酸Propionic acid (mg·mL-1) | 0.24±0.04c | 0.40±0.06b | 0.69±0.49b | 0.84±0.45a | 0.101 | 0.049 |
pH | 4.92±0.06a | 4.65±0.04b | 4.53±0.03c | 4.62±0.04b | 0.706 | 0.044 |
氨态氮NH3-N (g·kg-1) | 2.86±0.13a | 2.23±0.13c | 2.60±0.23ab | 2.42±0.19bc | 0.082 | 0.031 |
干物质DM (g·kg-1) | 45.84±0.21a | 45.73±0.15a | 43.45±0.11c | 44.22±0.21b | 0.309 | 0.032 |
粗蛋白CP (g DM·kg-1) | 18.36±0.21c | 19.22±0.19a | 18.79±0.16b | 19.27±0.15a | 0.119 | 0.032 |
粗纤维CF (g DM·kg-1) | 17.00±0.20b | 16.97±0.15b | 17.23±0.15ab | 17.37±0.15a | 0.065 | 0.027 |
酸性洗涤纤维ADF (g DM·kg-1) | 24.63±0.12c | 25.07±0.12b | 26.10±0.20a | 24.77±0.21bc | 0.162 | 0.028 |
中性洗涤纤维NDF (g DM·kg-1) | 24.43±0.15c | 25.93±0.21a | 25.00±0.17b | 24.53±0.15c | 0.157 | 0.030 |
可溶性碳水化合物WSC (g DM·kg-1) | 2.48±0.09b | 3.80±0.01a | 3.83±0.02a | 3.88±0.02a | 0.178 | 0.002 |
粗脂肪EE (g DM·kg-1) | 3.51±0.08a | 3.56±1.00a | 3.69±0.61a | 3.46±0.18a | 0.055 | 0.067 |
Table 1 Effect of carbendazim on the quality and nutritional value of white clover silage in orchard
项目Item | 空白对照 CON | RU- | RU | RU+ | 标准误SEM | P-value |
---|---|---|---|---|---|---|
乳酸Lactic acid (mg·mL-1) | 2.04±0.09c | 3.01±0.20b | 3.33±0.11a | 3.49±0.10a | 0.172 | 0.017 |
乙酸Acetic acid (mg·mL-1) | 0.49±0.05c | 0.61±0.04b | 0.71±0.06a | 0.72±0.04a | 0.738 | 0.030 |
丙酸Propionic acid (mg·mL-1) | 0.24±0.04c | 0.40±0.06b | 0.69±0.49b | 0.84±0.45a | 0.101 | 0.049 |
pH | 4.92±0.06a | 4.65±0.04b | 4.53±0.03c | 4.62±0.04b | 0.706 | 0.044 |
氨态氮NH3-N (g·kg-1) | 2.86±0.13a | 2.23±0.13c | 2.60±0.23ab | 2.42±0.19bc | 0.082 | 0.031 |
干物质DM (g·kg-1) | 45.84±0.21a | 45.73±0.15a | 43.45±0.11c | 44.22±0.21b | 0.309 | 0.032 |
粗蛋白CP (g DM·kg-1) | 18.36±0.21c | 19.22±0.19a | 18.79±0.16b | 19.27±0.15a | 0.119 | 0.032 |
粗纤维CF (g DM·kg-1) | 17.00±0.20b | 16.97±0.15b | 17.23±0.15ab | 17.37±0.15a | 0.065 | 0.027 |
酸性洗涤纤维ADF (g DM·kg-1) | 24.63±0.12c | 25.07±0.12b | 26.10±0.20a | 24.77±0.21bc | 0.162 | 0.028 |
中性洗涤纤维NDF (g DM·kg-1) | 24.43±0.15c | 25.93±0.21a | 25.00±0.17b | 24.53±0.15c | 0.157 | 0.030 |
可溶性碳水化合物WSC (g DM·kg-1) | 2.48±0.09b | 3.80±0.01a | 3.83±0.02a | 3.88±0.02a | 0.178 | 0.002 |
粗脂肪EE (g DM·kg-1) | 3.51±0.08a | 3.56±1.00a | 3.69±0.61a | 3.46±0.18a | 0.055 | 0.067 |
测序样本名称 Sequencing sample name | 原始PE reads数目 PE_reads | 去除嵌合体后有效序列数目 Nochimera | 有效序列平均长度 AvgLen (bp) | 各样品OTU总数OTUs |
---|---|---|---|---|
不喷施多菌灵(0 d,A) | 70933 | 66907 | 462 | 82 |
不喷施多菌灵(3 d,B) | 63698 | 54955 | 463 | 83 |
喷施多菌灵(3 d,C) | 59416 | 53199 | 463 | 87 |
喷施多菌灵(60 d,D) | 79630 | 68540 | 463 | 90 |
不喷施多菌灵(60 d,E) | 63874 | 58218 | 462 | 91 |
Table 2 Data quality statistics after optimization
测序样本名称 Sequencing sample name | 原始PE reads数目 PE_reads | 去除嵌合体后有效序列数目 Nochimera | 有效序列平均长度 AvgLen (bp) | 各样品OTU总数OTUs |
---|---|---|---|---|
不喷施多菌灵(0 d,A) | 70933 | 66907 | 462 | 82 |
不喷施多菌灵(3 d,B) | 63698 | 54955 | 463 | 83 |
喷施多菌灵(3 d,C) | 59416 | 53199 | 463 | 87 |
喷施多菌灵(60 d,D) | 79630 | 68540 | 463 | 90 |
不喷施多菌灵(60 d,E) | 63874 | 58218 | 462 | 91 |
1 | Qin W L. Functions of growing grass in orchard in the construction of rural complex. Journal of Hebei Agricultural Sciences, 2019, 23(6): 13-17. |
秦文利. 果园生草在田园综合体建设中的功能. 河北农业科学, 2019, 23(6): 13-17. | |
2 | Yang L, Mao Y F, Hu Y L, et al. Effects of orchard grass on soil fertility and apple tree nutrition. Journal of Plant Nutrition and Fertilizer, 2020, 26(2): 325-337. |
杨露, 毛云飞, 胡艳丽, 等. 生草改善果园土壤肥力和苹果树体营养的效果. 植物营养与肥料学报, 2020, 26(2): 325-337. | |
3 | Li Y P, Ren Q Q, Zhang J W. Effect of growing grass on soil nutrients in orchard. Yantai Fruits, 2020, 4: 20-21. |
李元鹏, 任倩倩, 张京伟. 生草对果园土壤养分的影响. 烟台果树, 2020, 4: 20-21. | |
4 | Gou M C. Evaluation of nitrogen metabolism ability of dominant grass species in apple orchard and effect of orchard management on soil nutrients. Shenyang: Shenyang Agricultural University, 2019. |
苟铭川. 自然生草苹果园优势草氮素代谢能力及刈割管理对土壤养分的影响. 沈阳: 沈阳农业大学, 2019. | |
5 | Fan X L, Wen S Y, Chen J, et al. Effect of C/N ratio on the fertility of white clover and wheat straw compost. Acta Agriculturae Boreali-Occidentalis Sinica, 2018, 27(9): 1322-1327. |
范肖龙, 文素芸, 陈佳, 等. 碳氮比对白三叶和小麦秸秆堆肥的肥力影响. 西北农业学报, 2018, 27(9): 1322-1327. | |
6 | Sholberg P L, Hogue E J, Neilsen G H. Effect of orchard cover crop on incidence of low-temperature-basidiomycete rot of stored Spartan apples. Canadian Journal of Plant Science, 1998, 78(1): 125-129. |
7 | Ripoche A, Aurélie Metay, Celette F, et al. Changing the soil surface management in vineyards: Immediate and delayed effects on the growth and yield of grapevine. Plant and Soil, 2011, 339(1/2): 259-271. |
8 | Huang T, Ding T, Liu D, et al. Degradation of carbendazim in soil: Effect of sewage sludge derived biochars. Journal of Agricultural and Food Chemistry, 2020, 68(12): 3703-3710. |
9 | Wei Z H, Xu J, Guo M X, et al. Research progress of carbendazim in China. Journal of Anhui Agricultural Sciences, 2015, 43(3): 125-127. |
魏中华, 徐娟, 郭明霞, 等. 国内多菌灵的研究进展. 安徽农业科学, 2015, 43(3): 125-127. | |
10 | Dai H L, Tian X H, Du W H, et al. Effects of silage additives on nutritional quality and silage quality of triticale and rye. Acta Prataculturae Sinica, 2019, 28(12): 213-221. |
代寒凌, 田新会, 杜文华, 等. 不同添加剂处理对小黑麦和黑麦青贮营养品质和发酵品质的影响. 草业学报, 2019, 28(12): 213-221. | |
11 | Han Z Y, Wang Z Y, Liu Y N, et al. The effects of different additives on pH and crude protein content in corn straw silage. Feed Review, 2019, 7: 22-25. |
韩紫燕, 王忠艳, 刘亚楠, 等. 不同添加剂对玉米秸秆青贮料pH及粗蛋白质含量的影响. 饲料博览, 2019, 7: 22-25. | |
12 | Wan X R, Dou S Y, Li Y, et al. Effect of lactic acid bacteria preparations on microbial population counts and silage quality in maize silage during fermentation and on aerobic exposure. Acta Prataculturae Sinica, 2020, 29(11): 83-90. |
万学瑞, 豆思远, 李玉, 等. 复合乳酸菌对全株玉米青贮及有氧暴露后微生物及饲料品质的影响. 草业学报, 2020, 29(11): 83-90. | |
13 | Huang F, Zhang L, Zhou B, et al. Research progress of silage microorganisms and their influence on the aerobic stability of silage. Acta Zoonutrimenta Sinica, 2019, 31(1): 93-100. |
黄峰, 张露, 周波, 等. 青贮微生物及其对青贮饲料有氧稳定性影响的研究进展. 动物营养学报, 2019, 31(1): 93-100. | |
14 | Ruediger G A, Pardon K H, Sas A N, et al. Fate of pesticides during the winemaking process in relation to malolactic fermentation. Journal of Agricultural and Food Chemistry, 2005, 53(8): 3023-3026. |
15 | Regueiro J, Olalla López-Fernández, Rial-Otero R, et al. A review on the fermentation of foods and the residues of pesticides biotransformation of pesticides and effects on fermentation and food quality. Critical Reviews in Food Science and Nutrition, 2015, 55(6): 839-863. |
16 | Xu D M, Zhang P, Ke W C, et al. Research progress of silage microorganisms and its effects on silage quality. Acta Agrestia Sinica, 2017, 25(3): 460-465. |
许冬梅, 张萍, 柯文灿, 等. 青贮微生物及其对青贮饲料发酵品质影响的研究进展. 草地学报, 2017, 25(3): 460-465. | |
17 | Silambarasan S, Abraham J. Biodegradation of carbendazim by a potent novel Chryseobacterium sp. JAS14 and plant growth promoting Aeromonas caviae JAS15 with subsequent toxicity analysis. 3 Biotech, 2020, 10(7): 137-140. |
18 | Wang X G, Wang Y Q, Yan H, et al. Effects of repeated application of carbendazim on its persistence and functional diversity of soil microbial communities. Acta Pedlolgica Sinica, 2010, 47(1): 131-137. |
王秀国, 王一奇, 严虎, 等. 多菌灵重复施药对其持久性及土壤微生物群落功能多样性的影响. 土壤学报, 2010, 47(1): 131-137. | |
19 | Singh S, Singh N, Kumar V, et al. Toxicity, monitoring and biodegradation of the fungicide carbendazim. Environmental Chemistry Letters, 2016, 14(3): 317-329. |
20 | Abolmohammad B, Asghar B, Damalas C A, et al. Use of personal protective equipment towards pesticide exposure: Farmers’ attitudes and determinants of behavior. Science of the Total Environment, 2018, 639: 1156-1163. |
21 | Yin X, Wang Y Q, Li X Q, et al. Effects of various moisture-absorbing roughages on the fermentation quality and aerobic stability of napier grass silage. Acta Prataculturae Sinica, 2021, 30(7): 133-138. |
尹祥, 王咏琪, 李鑫琴, 等. 不同水分吸附材料对象草青贮发酵品质及好氧稳定性的影响. 草业学报, 2021, 30(7): 133-138. | |
22 | Zhao M A, Feng Y N, Zhu Y Z, et al. Multi-residue method for determination of 238 pesticides in Chinese cabbage and cucumber by liquid chromatography tandem mass spectrometry: Comparison of different purification procedures. Journal of Agricultural and Food Chemistry, 2014, 62(47): 11449-11456. |
23 | He L W, Chen N, Lv H J, et al. Gallic acid influencing fermentation quality, nitrogen distribution and bacterial community of high-moisture mulberry leaves and stylo silage. Bioresource Technology, 2019, 295: 1-32. |
24 | Su R, Ni K, Wang T, et al. Effects of ferulic acid esterase-producing Lactobacillus fermentum and cellulase additives on the fermentation quality and microbial community of alfalfa silage. Peer Journal, 2019, 7: 7712. |
25 | Gharechahi J, Kharazian Z A, Sarikhan S, et al. The dynamics of the bacterial communities developed in maize silage. Microbial Biotechnology, 2017, 10(6): 1663-1676. |
26 | He Q, Huang J W, Yang X W, et al. Effect of pesticide residues in grapes on alcoholic fermentation and elimination of chlorothalonil inhibition by chlorothalonil hydrolytic dehalogenase. Food Control, 2016, 64: 70-76. |
27 | Junges D, Schmidt P, Novinski C O, et al. Additive containing homo and heterolactic bacteria on the fermentation quality of maize silage. Acta Scientiarum Animal Sciences, 2013, 35(4): 371-377. |
28 | Li R R, Jiang D, Tian P J, et al. Effects of different varieties and cutting time on fermentation quality and protein degradation of alfalfa silage. Chinese Journal of Grassland, 2020, 235(3): 97-104. |
李荣荣, 江迪, 田朋姣, 等. 不同品种和刈割时间对苜蓿青贮发酵品质及蛋白质降解的影响. 中国草地学报, 2020, 235(3): 97-104. | |
29 | Yang F Y. Breeding of cellulolytic lactic acid bacteria. Zhengzhou: Zhengzhou University, 2018. |
杨逢源. 纤维降解功能乳酸菌的选育. 郑州: 郑州大学, 2018. | |
30 | Fang H, Han L, Cui Y, et al. Changes in soil microbial community structure and function associated with degradation and resistance of carbendazim and chlortetracycline during repeated treatments. Science of the Total Environment, 2016, 572: 1203-1212. |
31 | Kato S, Haruta S, Cui Z J, et al. Stable coexistence of five bacterial strains as a cellulose-degrading community. Applied and Environmental Microbiology, 2005, 71(11): 7099-7106. |
32 | Wei Z H, Xu J, Guo M X, et al. Research progress of carbendazim in China. Agrochemicals Today, 2015, 11: 18-21. |
魏中华, 徐娟, 郭明霞, 等. 国内多菌灵的研究进展. 今日农药, 2015, 11: 18-21. | |
33 | Pu D, Zhang X Y, Liu G, et al. Identification and degrading bacteria GRPD-1 carbendazim degradation characteristics. Journal of Sichuan Normal University (Natural Science Edition), 2013, 36(2): 289-295. |
蒲丹, 张晓喻, 刘刚, 等. 多菌灵降解菌 GRPD-1 的鉴定及降解特性研究. 四川师范大学学报(自然科学版), 2013, 36(2): 289-295. | |
34 | Xu J L, Gu X Y, Shen B, et al. Isolation and characterization of a carbendazim-degrading rhodococcus sp.djl-6. Current Microbiology, 2006, 53(1): 72-76. |
35 | Dord E, Tijana M, Durovic P, et al. The potency of Saccharomyces cerevisiae and Lactobacillus plantarumto dissipate organophosphorus pesticides in wheat during fermentation. Journal of Food Science and Technology, 2016, 53(12): 4205-4215. |
36 | Li J, Wang Y R, Nian H H, et al. The isolation, identification and degradation character of a novel carbendazim-degrading bacterium. Journal of Huaibei Normal University (Natural Science Edition), 2017, 38(3): 37-42. |
李杰, 王亦然, 年浩瀚, 等. 一株新的多菌灵降解菌的筛选、鉴定及其降解特性. 淮北师范大学学报(自然科学版), 2017, 38(3): 37-42. | |
37 | Ma Z Z, Cheng Y Y, Wang S Q, et al. Positive effects of dietary supplementation of three probiotics on milk yield, milk composition and intestinal flora in Sannan dairy goats varied in kind of probiotics. Journal of Animal Physiology and Animal Nutrition, 2020, 104(3): 44-55. |
38 | Feld L, Hjelms M H, Nielsen M S, et al. Pesticide side effects in an agricultural soil ecosystem as measured by AMOA expression quantification and bacterial diversity changes. PLoS One, 2015, 10(5): e0126080. |
39 | Zhang Q, Yu Z, Wang X, et al. Effects of chlorpyrifos and chlorantraniliprole on fermentation quality of alfalfa (Medicago sativa L.) silage inoculated with or without Lactobacillus plantarum LP. Animal Science Journal, 2017, 88(3): 456-462. |
40 | Xiao L, Feng Y Y, Zhao L, et al. Effect of carbendazim on bacterial genetic diversity of soil. Xinjiang Agricultural Sciences, 2011, 48(9): 1640-1648. |
肖丽, 冯燕燕, 赵靓, 等. 多菌灵对土壤细菌遗传多样性的影响. 新疆农业科学, 2011, 48(9): 1640-1648. | |
41 | Fang H, Wang Y, Gao C, et al. Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim. Biodegradation, 2010, 21(6): 939-946. |
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