Genome-wide identification and expression profile analysis of the CPP gene family in Medicago truncatula

doi:10.11686/cyxb2021215

Abstract

Abstract:

The CPP （cysteine-rich polycomb-like protein） gene family is one category of small transcription factors occurring in a wide range of organisms， excepting yeasts and prokaryotes， and which play an important role in plant growth and development and in response to stress processes. The CPP gene family has been identified and studied in many species but so far no research in the model legume species Medicago truncatula has been reported. In this study， 9 CPP genes were identified in M. truncatula by bioinformatics. By constructing phylogenetic trees with the CPP protein sequences of three species， CPP genes could be divided into three categories. The genetic relationship between MtCPP members and soybean was closer. Conserved domain analysis showed that MtCPP transcription factors all had 1-2 CXC conserved domains. Chromosome mapping analysis found that 9 CPP genes were distributed on 6 chromosomes， and two pairs of paralogous genes were identified， which both derived from fragment duplication events. Combining these results with the analysis of gene chip expression database of M. truncatula， the expression of MtCPP genes had certain temporal and spatial specificity. MtCPP gene promoters contain a large number of cis acting elements related to hormone signalling and stress， MtCPP2，MtCPP5， MtCPP6 and MtCPP7 genes have expression characteristics of response to drought， and MtCPP2 and MtCPP8 genes may be involved in salt stress response. This study provides a research foundation for further functional analysis and screening of MtCPP genes involved in stress resistance in alfalfa.

Key words: CPP, transcription factor, Medicago truncatula, expression analysis, abiotic stress

Jiao-yang TIAN, Qiu-xia WANG, Shu-wen ZHENG, Wen-xian LIU. Genome-wide identification and expression profile analysis of the CPP gene family in Medicago truncatula[J]. Acta Prataculturae Sinica, 2022, 31(7): 111-121.

Figures/Tables 9

References 36

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序号 No.	基因名称 Gene name	Phytozome号 Phytozome ID	编码序列 Coding sequence （bp）	蛋白质 Protein （AA）	染色体定位 Chromosomal location	分子量 Molecular weight （Da）	等电点 Isoelectric point	亲水指数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
1	MtCPP1	Medtr1g012020.1	2604	867	chr1：2312974..2319368 （-）	93893.24	5.62	-0.670	细胞核 Nucleus
2	MtCPP2	Medtr1g086980.1	2496	831	chr1：38931314..38941671 （+）	93501.18	7.97	-0.775	细胞核 Nucleus
3	MtCPP3	Medtr1g103180.1	1101	366	chr1：46677103..46682000 （-）	39979.06	4.60	-0.674	细胞核 Nucleus
4	MtCPP4	Medtr1g103230.1	1875	624	chr1：46702340..46705795 （-）	70071.61	6.85	-0.802	细胞核 Nucleus
5	MtCPP5	Medtr2g049305.1	924	307	chr2：21725271..21728509 （-）	34610.89	8.50	-0.169	叶绿体Chloroplast
6	MtCPP6	Medtr3g110122.1	1791	596	chr3：51048745..51054541 （-）	65811.15	8.93	-0.493	细胞核 Nucleus
7	MtCPP7	Medtr5g006530.1	2337	778	chr5：955306..960319 （-）	85345.06	5.55	-0.693	细胞核 Nucleus
8	MtCPP8	Medtr6g087590.1	1824	607	chr6：32942677..32948999 （+）	65195.40	6.79	-0.550	细胞核 Nucleus
9	MtCPP9	Medtr8g103320.1	1437	478	chr8：43471929..43476739 （+）	51756.42	8.12	-0.648	细胞核 Nucleus

序号 No.	基因名称 Gene name	Phytozome号 Phytozome ID	编码序列 Coding sequence （bp）	蛋白质 Protein （AA）	染色体定位 Chromosomal location	分子量 Molecular weight （Da）	等电点 Isoelectric point	亲水指数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
1	MtCPP1	Medtr1g012020.1	2604	867	chr1：2312974..2319368 （-）	93893.24	5.62	-0.670	细胞核 Nucleus
2	MtCPP2	Medtr1g086980.1	2496	831	chr1：38931314..38941671 （+）	93501.18	7.97	-0.775	细胞核 Nucleus
3	MtCPP3	Medtr1g103180.1	1101	366	chr1：46677103..46682000 （-）	39979.06	4.60	-0.674	细胞核 Nucleus
4	MtCPP4	Medtr1g103230.1	1875	624	chr1：46702340..46705795 （-）	70071.61	6.85	-0.802	细胞核 Nucleus
5	MtCPP5	Medtr2g049305.1	924	307	chr2：21725271..21728509 （-）	34610.89	8.50	-0.169	叶绿体Chloroplast
6	MtCPP6	Medtr3g110122.1	1791	596	chr3：51048745..51054541 （-）	65811.15	8.93	-0.493	细胞核 Nucleus
7	MtCPP7	Medtr5g006530.1	2337	778	chr5：955306..960319 （-）	85345.06	5.55	-0.693	细胞核 Nucleus
8	MtCPP8	Medtr6g087590.1	1824	607	chr6：32942677..32948999 （+）	65195.40	6.79	-0.550	细胞核 Nucleus
9	MtCPP9	Medtr8g103320.1	1437	478	chr8：43471929..43476739 （+）	51756.42	8.12	-0.648	细胞核 Nucleus

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