Physiological responses of female and male Buchloe dactyloides plants to different nitrogen forms

doi:10.11686/cyxb2022035

Abstract

Abstract:

The aim of this study was to explore the preference and responses of female and male buffalograss （Buchloe dactyloides） to different nitrogen forms， and provide technical data for optimal management of nitrogen fertilizer for B. dactyloides. Thus we investigated the effects of NO₃^--N （N₁）， NH₄⁺-N （N₂）， NO₃^--N∶NH₄⁺-N=1∶1 （N₃） and a zero nitrogen treatment （CK） on growth traits， biomass， chlorophyll content， leaf nitrogen content and enzymes related to nitrogen metabolism of female and male buffalograss ramets in a glasshouse sand culture experiment. It was found that growth traits， biomass of different parts， aboveground biomass and total biomass of female buffalograss were significantly lower under the N₂ treatment than under N₁ and CK， with the result for N₃ being intermediate between N₁ and N₂ treatment， indicating that treatment with NH₄⁺-N alone may inhibit the growth of female buffalograss. Meanwhile， there was no significant difference between the N₁ treatment and CK in spacer length and diameter of male buffolagrass plants， but values for the N₁ treatment were significantly greater than those under N₂and N₃ treatments. Additionally， stolon node number， stolon length， root biomass， aboveground biomass and total biomass of male buffalograss were significantly greater under N₁ than other treatments. Chlorophyll A content and total chlorophyll content of female and male buffolagrass were greater under the NO₃^--N treatment than CK. However， there was no significant difference between the three N-form treatments for female buffalograss， but male buffalograss under the N₁ treatment differed significantly from N₂ and N₃ treatments. In the NH₄NO₃ treatment， leaf nitrogen content and three nitrogen metabolism related enzymes activities of female buffalograss were the highest， however， those of male buffalograss were highest under the N₁ treatment. These results indicate that both female and male buffalograss plants prefer nitrate nitrogen； female buffalograss plants were found to be extremely sensitive to NH₄⁺-N as the sole nitrogen source and their growth was inhibited， but mixed supply with ammonium and nitrate nitrogen alleviated or even improved the physiological function of N metabolism of female buffalograss； this response was not observed in male buffalograss. In summary， significant differences in the response of female and male buffalograss plants to different forms of nitrogen were found. This information could form a theoretical foundation for development of a differential nitrogen management of female and male buffolagrass.

Key words: Buchloe dactyloides, female and male, nitrogen form, biomass allocation, enzymes related to nitrogen metabolism

Li-zhu GUO, Hui-zhen MENG, Xi-feng FAN, Ke TENG, Wen-jun TENG, Hai-feng WEN, Yue-sen YUE, Hui ZHANG, Ju-ying WU. Physiological responses of female and male Buchloe dactyloides plants to different nitrogen forms[J]. Acta Prataculturae Sinica, 2023, 32(2): 65-74.

Figures/Tables 6

References 41

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项目 Item	对照 N-free （CK）	硝态氮 NO₃-N （N₁）	铵态氮 NH₄⁺-N （N₂）	混合态氮 NO₃-N∶NH₄⁺-N=1∶1 （N₃）
氯化钾 KCl	1.25	-	1.5	0.25
六水氯化钙 CaCl₂·6H₂O	1.25	-	1.25	-
七水硫酸镁 MgSO₄·7H₂O	0.5	0.5	0.5	0.5
磷酸二氢钾 KH₂（PO₄）	0.25	0.25	-	-
硝酸钾 KNO₃	-	1.25	-	1.25
四水硝酸钙 Ca（NO₃）₂·4H₂O	-	1.25	-	1.25
磷酸二氢铵 NH₄H₂PO₄	-	-	0.25	0.25
氯化铵 NH₄Cl	-	-	3.5	3.5
硼酸 H₃BO₃	0.0116	0.0116	0.0116	0.0116
四水氯化锰 MnCl₂·4H₂O	0.0046	0.0046	0.0046	0.0046
七水硫酸锌 ZnSO₄·7H₂O	0.00019	0.00019	0.00019	0.00019
钼酸钠 Na₂MoO₄	0.00012	0.00012	0.00012	0.00012
五水硫酸铜 CuSO₄·5H₂O	0.00008	0.00008	0.00008	0.00008
七水硫酸亚铁 FeSO₄·7H₂O	0.0125	0.0125	0.0125	0.0125
乙二酸四乙胺二钠 Na₂EDTA	0.0125	0.0125	0.0125	0.0125

项目 Item	对照 N-free （CK）	硝态氮 NO₃-N （N₁）	铵态氮 NH₄⁺-N （N₂）	混合态氮 NO₃-N∶NH₄⁺-N=1∶1 （N₃）
氯化钾 KCl	1.25	-	1.5	0.25
六水氯化钙 CaCl₂·6H₂O	1.25	-	1.25	-
七水硫酸镁 MgSO₄·7H₂O	0.5	0.5	0.5	0.5
磷酸二氢钾 KH₂（PO₄）	0.25	0.25	-	-
硝酸钾 KNO₃	-	1.25	-	1.25
四水硝酸钙 Ca（NO₃）₂·4H₂O	-	1.25	-	1.25
磷酸二氢铵 NH₄H₂PO₄	-	-	0.25	0.25
氯化铵 NH₄Cl	-	-	3.5	3.5
硼酸 H₃BO₃	0.0116	0.0116	0.0116	0.0116
四水氯化锰 MnCl₂·4H₂O	0.0046	0.0046	0.0046	0.0046
七水硫酸锌 ZnSO₄·7H₂O	0.00019	0.00019	0.00019	0.00019
钼酸钠 Na₂MoO₄	0.00012	0.00012	0.00012	0.00012
五水硫酸铜 CuSO₄·5H₂O	0.00008	0.00008	0.00008	0.00008
七水硫酸亚铁 FeSO₄·7H₂O	0.0125	0.0125	0.0125	0.0125
乙二酸四乙胺二钠 Na₂EDTA	0.0125	0.0125	0.0125	0.0125

处理 Treatments	雌株Female				雄株Male
处理 Treatments	全氮 Total nitrogen （%）	叶绿素a Chla （mg·g^-1）	叶绿素b Chlb （mg·g^-1）	叶绿素总量 Chl （mg·g^-1）	全氮 Total nitrogen （%）	叶绿素a Chla （mg·g^-1）	叶绿素b Chlb （mg·g^-1）	叶绿素总量 Chl （mg·g^-1）
CK	1.520±0.10c	0.620±0.08b	0.103±0.02	0.723±0.09b	0.799±0.07c	0.411±0.06c	0.202±0.02	0.613±0.08b
N₁	1.681±0.18bc	0.795±0.12a	0.128±0.02	0.924±0.14a	1.542±0.09a	0.610±0.11a	0.276±0.11	0.886±0.10a
N₂	1.832±0.15b	0.763±0.14ab	0.117±0.03	0.880±0.16ab	1.391±0.06b	0.546±0.09b	0.255±0.09	0.801±0.08ab
N₃	2.021±0.10a	0.723±0.09ab	0.116±0.01	0.838±0.10ab	1.448±0.09ab	0.477±0.07bc	0.229±0.02	0.705±0.09b

处理 Treatments	雌株Female				雄株Male
处理 Treatments	全氮 Total nitrogen （%）	叶绿素a Chla （mg·g^-1）	叶绿素b Chlb （mg·g^-1）	叶绿素总量 Chl （mg·g^-1）	全氮 Total nitrogen （%）	叶绿素a Chla （mg·g^-1）	叶绿素b Chlb （mg·g^-1）	叶绿素总量 Chl （mg·g^-1）
CK	1.520±0.10c	0.620±0.08b	0.103±0.02	0.723±0.09b	0.799±0.07c	0.411±0.06c	0.202±0.02	0.613±0.08b
N₁	1.681±0.18bc	0.795±0.12a	0.128±0.02	0.924±0.14a	1.542±0.09a	0.610±0.11a	0.276±0.11	0.886±0.10a
N₂	1.832±0.15b	0.763±0.14ab	0.117±0.03	0.880±0.16ab	1.391±0.06b	0.546±0.09b	0.255±0.09	0.801±0.08ab
N₃	2.021±0.10a	0.723±0.09ab	0.116±0.01	0.838±0.10ab	1.448±0.09ab	0.477±0.07bc	0.229±0.02	0.705±0.09b

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