Effects of selenium on metabolic transformation and morphogenesis in different varieties of alfalfa during the germination stage

doi:10.11686/cyxb2022141

Abstract

Abstract:

This research explored the effects of exogenous selenium on the metabolic transformations of sugar and nitrogen， germination indicators and morphogenesis in seeds of different varieties of alfalfa （Medicago sativa） during the germination stage. Five sodium selenite concentrations （0， 0.3， 0.6， 1.2， 2.4 mg·L^-1） were applied to M. sativa cv. Gannong No.3， DS310FY and M. sativa cv. Zhongmu No.5 seeds. The germination rate， phenotype， physiological indicators and selenium content of the treated seeds were determined after 10 days of culture， and multivariate and correlation analyses were used to explore the relationship between various physiological indicators and the phenotypic traits， so as to identify the most suitable selenium concentration and potentially define a methodology for producing selenium fortified materials. It was found that the reducing sugar content， soluble sugar content and germination potential of DS310FY and M. sativa cv. Gannong No.3 seedlings were all significantly higher than those of the control under 0.6 mg·L^-1 selenium treatment for 4 d （P<0.05）. The reducing sugar content， nitrate reductase activity， germination rate and germination index of DS310FY and M. sativa cv. Gannong No.3 seedlings were all significantly higher than those of the control under 0.3 and 0.6 mg·L^-1 selenium treatment for 10 d （P<0.05）， while the maximum values of epicotyl length of M. sativa cv. Gannong No.3 seedlings and total amino acid content， vigor index， epicotyl length， hypocotyl length， and fresh weight per plant of DS310FY alfalfa seedlings were all significantly higher after selenium application than in the control （P<0.05）. In addition， 0.3 mg·L^-1 selenium treatment also significantly increased sucrose content， germination rate and hypocotyl diameter of M. sativa Zhongmu No.5 seedlings compared to the control （P<0.05）. Correlation analysis and variation trend of each indicator showed that the accumulation and transformation of sugar in alfalfa seedlings mainly promoted the development of the hypocotyl， while the accumulation and transformation of nitrogen mainly promoted the elongation of the epicotyl and the increase in plant fresh weight. Therefore， increased sugar conversion efficiency after selenium application mainly promoted the germination of alfalfa seeds， while increased nitrogen conversion efficiency mainly promoted the morphological establishment of the seedlings. In conclusion， the overall performance of metabolic transformation and morphogenesis of DS310FY alfalfa during germination was best under 0.6 mg·L^-1 selenium treatment and this treatment also provided selenium nutrient fortification.

Key words: alfalfa, seed germination, selenium, substance transformation, morphogenesis

Shi-min ZHANG, Jiao-yang ZHAO, Hui-sen ZHU, Kai WEI, Yong-xin WANG. Effects of selenium on metabolic transformation and morphogenesis in different varieties of alfalfa during the germination stage[J]. Acta Prataculturae Sinica, 2023, 32(4): 79-90.

Figures/Tables 6

References 56

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处理 Treatments	指标 Index	甘农3号 Gannong No.3	DS310FY	中苜5号 Zhongmu No.5
GSY1， DSY2， ZMY1	发芽势Germination potential （%）	90.50±0.58a	90.25±1.71a	82.25±2.06b
GSY1， DSY2， ZMY1	发芽指数Germination index	206.55±1.77a	208.07±3.89a	161.77±4.17b
GSY1， DSY2， ZMY1	活力指数Vigor index	5.34±0.30a	5.58±0.32a	4.20±0.20b
GSY1， DSY2， ZMY1	硝酸还原酶Nitrate reductase （μg NO₂^-·g^-1FW·h^-1）	42.02±2.63a	36.64±2.01ab	33.71±3.40b
GSY1， DSY2， ZMY2	胚芽长Epicotyl length （mm）	35.94±2.14b	42.32±2.18a	43.95±3.82a
GSY2， DSY4， ZMCK	胚根长Hypocotyl length （mm）	19.10±1.55b	22.98±1.11a	24.42±1.15a
GSY2， DSY2， ZMY4	还原糖Reducing sugar of 4 d （%）	4.46±0.18b	5.45±0.29a	4.56±0.35b
GSY2， DSY1， ZMY2	还原糖Reducing sugar of 10 d （%）	3.02±0.13b	3.04±0.19b	3.91±0.25a
GSY3， DSY3， ZMY4	蔗糖Sucrose of 10 d （%）	4.11±0.20b	5.46±0.16a	5.34±0.25a
GSY1， DSY1， ZMY1	总氨基酸Total amino acid （μg·g^-1）	434.24±35.37b	512.62±21.15a	436.20±23.97b
GSY3， DSY1， ZMY1	可溶性糖Soluble sugar of 4 d （%）	1.72±0.11b	2.06±0.14a	2.19±0.10a

处理 Treatments	指标 Index	甘农3号 Gannong No.3	DS310FY	中苜5号 Zhongmu No.5
GSY1， DSY2， ZMY1	发芽势Germination potential （%）	90.50±0.58a	90.25±1.71a	82.25±2.06b
GSY1， DSY2， ZMY1	发芽指数Germination index	206.55±1.77a	208.07±3.89a	161.77±4.17b
GSY1， DSY2， ZMY1	活力指数Vigor index	5.34±0.30a	5.58±0.32a	4.20±0.20b
GSY1， DSY2， ZMY1	硝酸还原酶Nitrate reductase （μg NO₂^-·g^-1FW·h^-1）	42.02±2.63a	36.64±2.01ab	33.71±3.40b
GSY1， DSY2， ZMY2	胚芽长Epicotyl length （mm）	35.94±2.14b	42.32±2.18a	43.95±3.82a
GSY2， DSY4， ZMCK	胚根长Hypocotyl length （mm）	19.10±1.55b	22.98±1.11a	24.42±1.15a
GSY2， DSY2， ZMY4	还原糖Reducing sugar of 4 d （%）	4.46±0.18b	5.45±0.29a	4.56±0.35b
GSY2， DSY1， ZMY2	还原糖Reducing sugar of 10 d （%）	3.02±0.13b	3.04±0.19b	3.91±0.25a
GSY3， DSY3， ZMY4	蔗糖Sucrose of 10 d （%）	4.11±0.20b	5.46±0.16a	5.34±0.25a
GSY1， DSY1， ZMY1	总氨基酸Total amino acid （μg·g^-1）	434.24±35.37b	512.62±21.15a	436.20±23.97b
GSY3， DSY1， ZMY1	可溶性糖Soluble sugar of 4 d （%）	1.72±0.11b	2.06±0.14a	2.19±0.10a

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