Acta Prataculturae Sinica ›› 2024, Vol. 33 ›› Issue (6): 145-154.DOI: 10.11686/cyxb2023248
Yi-fan WANG(), Xing-liang ZHUO, Lei WANG, Hong-rui ZHANG, Xue CHEN, Fang-cai JI, Zhu YU()
Received:
2023-07-18
Revised:
2023-09-25
Online:
2024-06-20
Published:
2024-03-20
Contact:
Zhu YU
Yi-fan WANG, Xing-liang ZHUO, Lei WANG, Hong-rui ZHANG, Xue CHEN, Fang-cai JI, Zhu YU. Effect of harvest period and processing method on the quality and in vitro digestibility of native grass products[J]. Acta Prataculturae Sinica, 2024, 33(6): 145-154.
项目Item | T1 | T2 | T3 | T4 | T5 | P值 P-value |
---|---|---|---|---|---|---|
pH | 4.47±0.32a | 4.07±0.04c | 4.14±0.02bc | 4.06±0.06c | 4.35±0.05ab | 0.023 |
乳酸 Lactic acid (%DM) | 3.60±0.79ab | 3.16±1.06b | 4.90±0.68a | 4.88±0.24a | 2.93±0.59b | 0.017 |
乙酸Acetic acid (%DM) | 0.49±0.08b | 0.71±0.35ab | 1.41±0.55a | 1.45±0.60a | 1.02±0.30ab | 0.071 |
丙酸 Propionic acid (%DM) | 0.80±0.60ab | 0.21±0.15b | 0.98±0.37a | 0.18±0.11b | 0.68±0.40ab | 0.086 |
丁酸 Butyric acid (%DM) | 0.25±0.06ab | 0.15±0.02b | 0.13±0.04b | 0.25±0.17ab | 0.47±0.28a | 0.114 |
氨态氮Ammonia nitrogen (%TN) | 5.18±0.22ab | 5.55±1.46a | 4.19±0.29b | 4.24±0.06b | 4.14±0.23b | 0.087 |
Kaiser评分 Kaiser score | 100 | 100 | 100 | 100 | 80 |
Table 1 Effects of different harvest periods on the fermentation quality of native grass silage
项目Item | T1 | T2 | T3 | T4 | T5 | P值 P-value |
---|---|---|---|---|---|---|
pH | 4.47±0.32a | 4.07±0.04c | 4.14±0.02bc | 4.06±0.06c | 4.35±0.05ab | 0.023 |
乳酸 Lactic acid (%DM) | 3.60±0.79ab | 3.16±1.06b | 4.90±0.68a | 4.88±0.24a | 2.93±0.59b | 0.017 |
乙酸Acetic acid (%DM) | 0.49±0.08b | 0.71±0.35ab | 1.41±0.55a | 1.45±0.60a | 1.02±0.30ab | 0.071 |
丙酸 Propionic acid (%DM) | 0.80±0.60ab | 0.21±0.15b | 0.98±0.37a | 0.18±0.11b | 0.68±0.40ab | 0.086 |
丁酸 Butyric acid (%DM) | 0.25±0.06ab | 0.15±0.02b | 0.13±0.04b | 0.25±0.17ab | 0.47±0.28a | 0.114 |
氨态氮Ammonia nitrogen (%TN) | 5.18±0.22ab | 5.55±1.46a | 4.19±0.29b | 4.24±0.06b | 4.14±0.23b | 0.087 |
Kaiser评分 Kaiser score | 100 | 100 | 100 | 100 | 80 |
项目 Item | 加工方式 Processing method | 收割时间Harvest period | P值 P-value | ||||||
---|---|---|---|---|---|---|---|---|---|
T1 | T2 | T3 | T4 | T5 | T | M | T*M | ||
干物质 Dry matter (DM,%FW) | 青贮发酵Silage fermentation | 37.07±0.29aA | 32.98±0.13cB | 35.22±0.24bA | 30.17±0.96dA | 35.56±0.14bB | <0.001 | 0.033 | 0.061 |
天然干燥 Natural drying | 38.01±0.64aA | 36.23±1.28bcA | 34.94±0.73cA | 32.00±0.80dA | 37.20±0.32abA | ||||
人工干燥Artificial drying | 37.29±1.55aA | 34.59±0.40bAB | 35.48±0.72abA | 31.63±0.67cA | 35.65±1.59abAB | ||||
粗蛋白质 Crude protein (CP,%DM) | 青贮发酵Silage fermentation | 11.13±0.11dAB | 13.20±0.34cA | 14.74±0.81abA | 15.46±0.25aA | 14.28±0.68bA | <0.001 | 0.008 | 0.026 |
天然干燥 Natural drying | 10.17±0.70dB | 12.17±0.50cB | 13.11±0.21bB | 14.47±0.30aB | 12.62±0.09bcB | ||||
人工干燥Artificial drying | 12.56±1.37cA | 12.62±0.31cAB | 14.11±0.30bA | 15.61±0.35aA | 13.37±0.45bcA | ||||
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | 青贮发酵Silage fermentation | 40.39±0.75cC | 46.96±1.52aB | 44.48±1.27bAB | 41.33±1.13cA | 45.31±0.97abA | 0.009 | 0.023 | 0.241 |
天然干燥 Natural drying | 44.75±0.93bB | 49.71±2.00aAB | 42.18±1.25bcB | 40.25±1.52cA | 43.34±2.73bcA | ||||
人工干燥Artificial drying | 49.84±1.56bcA | 53.50±4.59aA | 46.09±2.15bcA | 41.33±0.48cA | 48.87±9.58bcA | ||||
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | 青贮发酵Silage fermentation | 26.11±0.34cB | 30.16±0.76abA | 28.20±0.68bcA | 28.16±0.81bcA | 30.52±2.18aA | <0.001 | 0.140 | 0.017 |
天然干燥 Natural drying | 28.77±0.66bcA | 31.11±1.43aA | 29.24±0.63abA | 26.98±0.47cA | 27.49±1.81bcA | ||||
人工干燥Artificial drying | 30.61±1.37abA | 36.47±7.02aA | 28.42±1.20bA | 26.59±0.74bA | 28.09±0.83bA | ||||
可溶性碳水化合物 Water-soluble carbohydrate (WSC,%DM) | 青贮发酵Silage fermentation | 2.16±0.64aC | 0.63±0.15bC | 1.02±0.25bC | 0.73±0.29bB | 0.95±0.46bB | 0.003 | <0.001 | 0.236 |
天然干燥 Natural drying | 5.42±0.60aB | 3.62±0.47bB | 3.78±1.00abB | 3.98±1.00abA | 4.56±1.00abA | ||||
人工干燥Artificial drying | 7.44±0.46aA | 5.10±0.06bcA | 5.83±0.05bA | 4.66±0.82cA | 4.91±0.50cA | ||||
粗脂肪 Ether extract (EE,%DM) | 青贮发酵Silage fermentation | 3.61±0.32bB | 3.62±0.17bB | 4.29±0.22aA | 4.61±0.31aA | 4.37±0.21aB | 0.020 | <0.001 | 0.289 |
天然干燥 Natural drying | 4.49±0.10aA | 4.98±0.69aA | 4.82±0.80aA | 5.24±0.40aA | 5.14±0.91aAB | ||||
人工干燥Artificial drying | 4.52±0.38bA | 5.00±0.85abA | 4.52±0.36bA | 4.58±0.54bA | 5.59±0.30aA |
Table 2 Effects of different harvesting periods and processing methods on the nutritional quality of native grass
项目 Item | 加工方式 Processing method | 收割时间Harvest period | P值 P-value | ||||||
---|---|---|---|---|---|---|---|---|---|
T1 | T2 | T3 | T4 | T5 | T | M | T*M | ||
干物质 Dry matter (DM,%FW) | 青贮发酵Silage fermentation | 37.07±0.29aA | 32.98±0.13cB | 35.22±0.24bA | 30.17±0.96dA | 35.56±0.14bB | <0.001 | 0.033 | 0.061 |
天然干燥 Natural drying | 38.01±0.64aA | 36.23±1.28bcA | 34.94±0.73cA | 32.00±0.80dA | 37.20±0.32abA | ||||
人工干燥Artificial drying | 37.29±1.55aA | 34.59±0.40bAB | 35.48±0.72abA | 31.63±0.67cA | 35.65±1.59abAB | ||||
粗蛋白质 Crude protein (CP,%DM) | 青贮发酵Silage fermentation | 11.13±0.11dAB | 13.20±0.34cA | 14.74±0.81abA | 15.46±0.25aA | 14.28±0.68bA | <0.001 | 0.008 | 0.026 |
天然干燥 Natural drying | 10.17±0.70dB | 12.17±0.50cB | 13.11±0.21bB | 14.47±0.30aB | 12.62±0.09bcB | ||||
人工干燥Artificial drying | 12.56±1.37cA | 12.62±0.31cAB | 14.11±0.30bA | 15.61±0.35aA | 13.37±0.45bcA | ||||
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | 青贮发酵Silage fermentation | 40.39±0.75cC | 46.96±1.52aB | 44.48±1.27bAB | 41.33±1.13cA | 45.31±0.97abA | 0.009 | 0.023 | 0.241 |
天然干燥 Natural drying | 44.75±0.93bB | 49.71±2.00aAB | 42.18±1.25bcB | 40.25±1.52cA | 43.34±2.73bcA | ||||
人工干燥Artificial drying | 49.84±1.56bcA | 53.50±4.59aA | 46.09±2.15bcA | 41.33±0.48cA | 48.87±9.58bcA | ||||
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | 青贮发酵Silage fermentation | 26.11±0.34cB | 30.16±0.76abA | 28.20±0.68bcA | 28.16±0.81bcA | 30.52±2.18aA | <0.001 | 0.140 | 0.017 |
天然干燥 Natural drying | 28.77±0.66bcA | 31.11±1.43aA | 29.24±0.63abA | 26.98±0.47cA | 27.49±1.81bcA | ||||
人工干燥Artificial drying | 30.61±1.37abA | 36.47±7.02aA | 28.42±1.20bA | 26.59±0.74bA | 28.09±0.83bA | ||||
可溶性碳水化合物 Water-soluble carbohydrate (WSC,%DM) | 青贮发酵Silage fermentation | 2.16±0.64aC | 0.63±0.15bC | 1.02±0.25bC | 0.73±0.29bB | 0.95±0.46bB | 0.003 | <0.001 | 0.236 |
天然干燥 Natural drying | 5.42±0.60aB | 3.62±0.47bB | 3.78±1.00abB | 3.98±1.00abA | 4.56±1.00abA | ||||
人工干燥Artificial drying | 7.44±0.46aA | 5.10±0.06bcA | 5.83±0.05bA | 4.66±0.82cA | 4.91±0.50cA | ||||
粗脂肪 Ether extract (EE,%DM) | 青贮发酵Silage fermentation | 3.61±0.32bB | 3.62±0.17bB | 4.29±0.22aA | 4.61±0.31aA | 4.37±0.21aB | 0.020 | <0.001 | 0.289 |
天然干燥 Natural drying | 4.49±0.10aA | 4.98±0.69aA | 4.82±0.80aA | 5.24±0.40aA | 5.14±0.91aAB | ||||
人工干燥Artificial drying | 4.52±0.38bA | 5.00±0.85abA | 4.52±0.36bA | 4.58±0.54bA | 5.59±0.30aA |
项目 Item | 加工方式 Processing method | 收割时间Harvest period | P值 P-value | ||||||
---|---|---|---|---|---|---|---|---|---|
T1 | T2 | T3 | T4 | T5 | T | M | T×M | ||
干物质体外消化率 In vitro dry matter digestibility (IVDMD) | 青贮发酵Silage fermentation | 56.27±2.07aA | 51.44±0.85bA | 53.43±1.38bA | 52.84±1.37bA | 53.27±1.47bA | 0.032 | <0.001 | 0.855 |
天然干燥 Natural drying | 51.97±0.55aB | 48.93±0.86bB | 48.21±0.06bB | 49.48±1.15bB | 48.92±1.40bB | ||||
人工干燥Artificial drying | 56.42±4.11aA | 51.11±3.46aAB | 53.99±6.19aA | 51.75±4.23aAB | 56.13±4.96aA | ||||
粗蛋白质体外消化率 In vitro crude protein digestibility (IVCPD) | 青贮发酵Silage fermentation | 59.62±0.96bA | 65.70±0.72aA | 61.08±1.37bA | 64.07±1.29aA | 59.05±1.38bB | <0.001 | <0.001 | 0.253 |
天然干燥 Natural drying | 56.07±0.55bB | 61.07±1.79aB | 57.40±1.49bB | 60.38±1.07aB | 56.23±0.59bC | ||||
人工干燥 Artificial drying | 59.09±4.48aAB | 63.91±0.92aA | 63.96±3.54aA | 63.34±4.72aAB | 63.89±2.31aA | ||||
中性洗涤纤维消化率 In vitro neutral detergent fiber digestibility (IVNDFD) | 青贮发酵Silage fermentation | 50.74±2.07aA | 46.53±2.11bA | 47.86±1.62abA | 47.68±2.53abA | 46.58±0.85bA | 0.003 | 0.554 | 0.609 |
天然干燥 Natural drying | 49.69±3.42aA | 47.83±2.41abA | 47.28±2.28abA | 45.46±2.03abA | 43.98±2.87bA | ||||
人工干燥Artificial drying | 50.26±0.85aA | 49.97±1.89aA | 45.16±4.76aA | 46.30±4.10aA | 44.75±1.17aA |
Table 3 Effects of different harvest periods and processing methods on in vitro digestibility (%)
项目 Item | 加工方式 Processing method | 收割时间Harvest period | P值 P-value | ||||||
---|---|---|---|---|---|---|---|---|---|
T1 | T2 | T3 | T4 | T5 | T | M | T×M | ||
干物质体外消化率 In vitro dry matter digestibility (IVDMD) | 青贮发酵Silage fermentation | 56.27±2.07aA | 51.44±0.85bA | 53.43±1.38bA | 52.84±1.37bA | 53.27±1.47bA | 0.032 | <0.001 | 0.855 |
天然干燥 Natural drying | 51.97±0.55aB | 48.93±0.86bB | 48.21±0.06bB | 49.48±1.15bB | 48.92±1.40bB | ||||
人工干燥Artificial drying | 56.42±4.11aA | 51.11±3.46aAB | 53.99±6.19aA | 51.75±4.23aAB | 56.13±4.96aA | ||||
粗蛋白质体外消化率 In vitro crude protein digestibility (IVCPD) | 青贮发酵Silage fermentation | 59.62±0.96bA | 65.70±0.72aA | 61.08±1.37bA | 64.07±1.29aA | 59.05±1.38bB | <0.001 | <0.001 | 0.253 |
天然干燥 Natural drying | 56.07±0.55bB | 61.07±1.79aB | 57.40±1.49bB | 60.38±1.07aB | 56.23±0.59bC | ||||
人工干燥 Artificial drying | 59.09±4.48aAB | 63.91±0.92aA | 63.96±3.54aA | 63.34±4.72aAB | 63.89±2.31aA | ||||
中性洗涤纤维消化率 In vitro neutral detergent fiber digestibility (IVNDFD) | 青贮发酵Silage fermentation | 50.74±2.07aA | 46.53±2.11bA | 47.86±1.62abA | 47.68±2.53abA | 46.58±0.85bA | 0.003 | 0.554 | 0.609 |
天然干燥 Natural drying | 49.69±3.42aA | 47.83±2.41abA | 47.28±2.28abA | 45.46±2.03abA | 43.98±2.87bA | ||||
人工干燥Artificial drying | 50.26±0.85aA | 49.97±1.89aA | 45.16±4.76aA | 46.30±4.10aA | 44.75±1.17aA |
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