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    20 June 2024, Volume 33 Issue 6
    Climate responses of carbon fluxes in two adjacent alpine grasslands in northern Tibet
    Ling-ling XU, Ben NIU, Xian-zhou ZHANG, Yong-tao HE, Pei-li SHI, Ning ZONG, Jian-shuang WU, Xiang-tao WANG
    2024, 33(6):  1-16.  DOI: 10.11686/cyxb2023250
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    The sensitivity of ecosystem carbon cycle to climate change is an important issue in the context of future carbon neutrality across the globe. Because of the vast area, rich carbon storage, and vulnerability of the alpine grassland on the Qinghai-Tibetan Plateau, many site-specific studies have focused on the responses of carbon and water fluxes of alpine grassland to climate change in recent years. In such studies, various observation and simulation techniques, especially eddy correlation (EC) techniques, have been used. However, the sensitivity of different alpine grassland types to changes in climatic factors under the same climatic background remains unclear. Based on the estimates of carbon and water fluxes made using EC techniques from 2009 to 2011 under different water conditions, this study analyzed and compared the climate sensitivity of the carbon cycle between two adjacent (<5 km) alpine grassland ecosystems, alpine meadow and alpine wetland, in northern Tibet. The results showed that the carbon exchange capacity of alpine meadow was significantly lower than that of alpine wetland. The alpine meadow was carbon neutral with an annual net CO2 exchange (NEE) of 18.09±40.66 g C·m-2, while the alpine wetland was a stable carbon sink with an annual NEE of 155.09±32.85 g C·m-2. The moisture conditions weakly affected the maximum photosynthetic rate of the light response curves in both alpine meadow and alpine wetland, but was significantly positively correlated with gross primary productivity under saturated light intensity. The effect of moisture conditions to regulate the exponential response relationship between ecosystem respiration (Re) and temperature was mainly reflected by the basic respiration value (a). The mean a value of alpine wetland was 3.76-times larger than that of alpine meadow, but the effect of moisture conditions to regulate the temperature sensitivity of Re (Q10) in the two alpine grasslands was not significant. The mean value of annual Q10 (1.84) and the trend of Q10 to decrease with temperature were basically the same in the alpine meadow and alpine wetland. We concluded that the response of CO2 exchange in the alpine grassland ecosystem in northern Tibet to environmental factors depends on joint restricting effects of multiple factors. Therefore, a regional network and collaborative observations are helpful to better understand the mechanism of the carbon exchange response of the alpine ecosystem to climate change.

    Effects of different grazing times on soil particle composition and fractal dimension in the desert steppe
    Hai-xin JIANG, Yao ZHOU, Ke HU, Zhan-sheng DING, Hong-bin MA
    2024, 33(6):  17-28.  DOI: 10.11686/cyxb2023243
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    Studying the impact of the timing of grazing on soil particle composition and fractal dimension in grasslands is crucial for understanding how grazing disturbances affect soil quality and the ecological conditions of grasslands. The overall aim of this research, therefore, was to provide a theoretical foundation for determining the most suitable timing of grazing in grassland ecosystems. This study was conducted on the desert steppe of Ningxia, and five grazing treatments were implemented: forbidden grazing (FY), traditional time rotational grazing (FG), delayed start rotational grazing (YG), early end rotational grazing (TG), and delayed start and early end rotational grazing (YT). We collected soil samples from all of these treatments. Then, we determined how different grazing timing influenced changes in soil particle composition and fractal dimension on the desert steppe and identified the factors driving these changes. The results clearly demonstrate that across all treatments, the primary components of soil texture in desert steppe were silt, very fine sand, and fine sand particles. We observed significant variations in soil particle composition among treatments within the 0-40 cm soil depth range (P<0.05). The soil particle fractal dimension ranged from 2.11 to 2.75, with a gradual increase with increasing soil depth. Notably, YG had the highest silt content and fractal dimension. The silt content and fractal dimension FY of 0-10 cm soil layer were the lowest, and the FG treatment was the lowest in the other soil layers. The soil fractal dimension was significantly positively correlated with silt content (P<0.01), and significantly negatively correlated with very fine sand and fine sand contents (P<0.01, P<0.05). The soil particle fractal dimension was positively correlated with organic carbon content and total porosity, and negatively correlated with bulk density, available potassium content, and capillary porosity. In conclusion, our results suggest that the fractal dimension can effectively serve as an indicator of changes in soil texture on the desert steppe. Delayed grazing practices positively influence soil particle composition and fractal dimension, ultimately improving soil quality. These findings offer valuable insights for determining the optimal timing of grazing on the desert steppe.

    Effect and mechanisms of alternative livelihood options for improving herder engagement with ecological compensation policy-a study of outcomes under China’s “Grassland ecological compensation policy”
    Liu-fang SU, Huan-guang QIU, Hui-fang LIU, Ling-ling HOU
    2024, 33(6):  29-46.  DOI: 10.11686/cyxb2023345
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    The implementation of China’s ecological compensation policy whereby herders are paid to control overgrazing to facilitate environmental protection, still faces the challenge of ineffectiveness. The constraint on livelihoods determined by resource limitations stands out as a significant factor contributing to the failure of the ecological compensation policy. Exploring ways to optimize the implementation and impact of ecological compensation through the lens of alternative livelihoods can offer a fresh approach to reconcile existing research on the connection between ecological compensation and sustaining livelihoods. Moreover, this approach can also contribute to furthering the reform efforts with respect to ecological compensation policy. This study, using data on outcomes under the grassland ecological compensation policy (GECP), conducted interval data regression and bivariate probit regression analyses using 2 years of tracking survey data from Gansu and Qinghai provinces. Building on the herders’ grazing prohibition behavioral experiment, this study empirically examined the impact of herders’ alternative livelihood opportunities over the past 6 years and village-level alternative livelihood measures over the past 4 years on their willingness to accept (WTA) compensation for grazing prohibition. Our study considers the perspectives of non-pastoral employment and employment training, with the aim of elucidating the enhancement effect of existence of alternative livelihood options on the effectiveness of the GECP. The findings reveal that the alternative livelihood opportunities of herders are crucial in determining their WTA grazing bans and optimizing the effectiveness of compensation policies under the current policy settings. However, only high-skilled non-pastoral employment with high income and stability can effectively contribute to improved WTA. Additionally, the provision of employment training services at the village level is beneficial in decreasing the WTA of herders and improving the effectiveness of GECP under the current compensation standards, although this effect requires a cumulative period of 3-4 years to become evident. Therefore, in the process of promoting ecological compensation reform, we should focus on balancing the ecological environment protection and the interests of the compensated subjects, fully consider the alternative livelihood transformation and development issues of the compensated subjects, provide them with more comprehensive and effective support, and promote construction of a social ethos with a strong ecological focus.

    Accounting and assessment of grassland ecological values
    Lin HUANG, Jia-hui LI, Hai-yan ZHANG, Yu-zhe LI, Sui-zi WANG, Jiang-wen FAN
    2024, 33(6):  47-63.  DOI: 10.11686/cyxb2023115
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    There are two main objectives when accounting for and assessing grassland ecological values. The first objective is to provide a theoretical basis for appropriate pricing, effective compensation, scientific management, and sustainable development of grassland resources. This allows for ecological benefits to be integrated into economic and social development decisions. The second objective is to provide a scientific method for evaluating the effectiveness of grassland ecological conservation and restoration, which allows for planners to change and influence the behavior and decisions of ecological conservation and restoration through monetization. Based on the theories of ecological economics and ecosystem assessment, we defined the grassland ecological value as the sum of various services, products, and benefits that can be directly provided to humans as determined by the structure and function of the grassland ecosystem. We developed a grassland ecological value assessment index system and determined spatial measurement methods for estimating the quality and value of each indicator. This allowed us to assess grassland ecological value and its dynamic changes in space and time. To estimate and assess the volume and value of grassland, volume estimation and evaluation methods were applied to Hulun Buir in the Inner Mongolia Autonomous Region. The results show that the total ecological value of grasslands in the city region was about RMB 262.87 billion yuan·yr-1, which was 2.2 times the city’s gross domestic product in 2020. The value of grassland species conservation accounted for 43% of the total grassland ecological value. The ecological value of the grassland unit area was about 39,100 yuan·ha-1·yr-1. This showed a spatially divergent pattern of gradual decline from west to east. These results highlight the need to understand the function and status of grasslands from the perspective of their ecological value, and to strengthen the scientific protection and rational use of grassland areas.

    Effects of root application of an arbuscular mycorrhizal fungus and melatonin on the growth, photosynthetic characteristics, and antioxidant system of Medicago sativa under salt stresss
    Ying TAN, Hao YIN
    2024, 33(6):  64-75.  DOI: 10.11686/cyxb2023366
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    The aim of this study was to investigate the effects of an arbuscular mycorrhizal fungus (AMF) and melatonin treatments on the photosynthetic parameters and antioxidant system of Medicago sativa (alfalfa) plants under salt stress. Melatonin was applied at a range of concentrations (0, 50, 100, 150, and 200 μmol·L-1) to roots of M. sativa under salt stress (100 mmol·L-1 NaCl). A total of 10 treatments were inoculated with an AMF (Glomus versiforme), whereas the control plants were left uninoculated. It was found that this AMF formed a stable symbiotic relationship with alfalfa, with a maximum mycorrhizal infection rate of 65.2%. Compared with the control group, the group inoculated with AMF and treated with melatonin at 100 μmol·L-1 showed increased total biomass (by 5.4%) and chlorophyll a and chlorophyll b contents (by 7.3% and 45.3%, respectively), a decreased root to shoot ratio (by 39.5%), and increased net photosynthetic rate, transpiration rate, intercellular CO2 concentration, and stomatal conductance (by 23.3%, 28.3%, 17.6%, and 14.1%, respectively). Compared with the control group, the group inoculated with AMF and treated with melatonin at 100 μmol·L-1 showed increased maximum photochemical efficiency and PS Ⅱ potential activity (by 19.6% and 22.9%, respectively); increased nitrogen, phosphorus, and potassium contents in the leaves (to 1.4-times, 1.1-times, and 1.2-times that in the control, respectively), and decreased sodium content (by 11.1%). Comparisons of gene transcript levels between the control group and the group inoculated with AMF and treated with melatonin at 100 μmol·L-1 revealed increased transcript levels of Cu/Zn-SOD (by 33.9%), POD (by 16.8%), and CAT (by 6.4%) in the latter group. The activities of superoxide dismutase, peroxidase, and catalase were increased to different degrees in the group inoculated with AMF and treated with melatonin at 100 μmol·L-1. We concluded that AMF and melatonin can synergistically increase the chlorophyll a and b contents and chlorophyll fluorescence parameters of alfalfa, improve photosynthesis, increase the absorption of nutrients such as nitrogen, phosphorus, and potassium, reduce the absorption of sodium, and upregulate the expression of Cu/Zn-SOD, POD, and CAT, leading to increased activities of superoxide dismutase, peroxidase, and catalase. Among the treatments tested here, AMF inoculation combined with root application of 100 μmol·L-1 melatonin had the strongest effect to promote plant growth under salt stress and improve salt resistance.

    Evaluation of physiological characteristics and cold resistance of 10 alfalfa varieties under low temperature stress
    Min WANG, Li LI, Rong JIA, Ai-ke BAO
    2024, 33(6):  76-88.  DOI: 10.11686/cyxb2023353
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    This research elucidated the physiological responses of 10 alfalfa (Medicago sativa) varieties from diverse regions to low temperature stress in order to identify highly cold-resistant varieties. Twelve physiological indexes of the alfalfa seedlings were measured under low temperature conditions of 4 °C, and the cold tolerance was assessed using correlation analysis, principal component analysis and membership function evaluation methods. Simultaneously, the cold resistance of the 10 alfalfa varieties was verified by a semi-lethal temperature (LT50). The key findings were: 1) Under a low temperature of 4 °C, the leaf transpiration rate of the 10 alfalfa varieties exhibited varying degrees of reduction compared to the control group. Decreased transpiration was accompanied by an increase in relative conductivity, as well as in the levels of proline (Pro), soluble sugar, soluble protein (SP), superoxide dismutase (SOD) and peroxidase (POD) activity. 2) The multivariate cold tolerance ranking of the 10 alfalfa varieties using the membership function method was as follows: Longmu 801 (highest)>Zhaodong>Bara 520 YQ>Gongnong 1>Baimu 401>Aohan>Challenger>Dongnong 1>Knights and finally, Xinmu 4. Further measurement of the LT50 for the leaves of each variety confirmed that Longmu 801 and Xinmu 4 were the varieties with the lowest and highest LT50 values respectively, thus corroborating the result of the membership function evaluation. 3) Regression analysis was used to establish an evaluation model for the cold resistance of alfalfa seedlings, and its multiple-parameter evaluation value (D) was: 0.138+0.178×SP+0.203×POD+0.170×Pro, highlighting SP, POD, and Pro as pivotal physiological indicators of cold resistance in alfalfa seedlings. Overall, this research provides fundamental insights into the mechanism of low-temperature adaptation in alfalfa seedlings and will facilitate the selection of cold-resistant alfalfa varieties.

    Effects of straw return combined with phosphorus fertilizer on carbon assimilate accumulation in dryland wheat and the associated physiological mechanisms
    Jun-hao ZHANG, Xue-ru CHAI, Song-ke MA, Dong-xia ZHANG, Jing ZHANG, Chang-chang QIAO, Shuang LI, Ming HUANG, He-zheng WANG
    2024, 33(6):  89-104.  DOI: 10.11686/cyxb2023282
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    The purpose of this study was to clarify the effects of straw return combined with phosphate (P) fertilizer on the characteristics of carbon assimilation accumulation and grain yield in wheat (Triticum aestivum). In 2021-2022, a split-spilt-plot field experiment with Luohan22 used as the tested wheat variety was conducted in typical drylands of western Henan province. In this experiment, two corn (Zea mays) straw return treatments (no straw return, S0; full straw return, S1) were set up as main plot treatments, and five rates of P fertilizer application rates (0 kg·ha-1, P0; 75 kg·ha-1, P1; 112.5 kg·ha-1, P2; 150 kg·ha-1, P3; 187.5 kg·ha-1, P4), were applied as split-plot treatments. We investigated the characteristics of dry matter accumulation, grain yield and its components in wheat, and the changes of net photosynthetic rate (Pn) and leaf chlorophyll measured by soil and plant analyzer development (SPAD) meter value in flag leaves, and the soluble sugar content, sucrose content and sucrase activity in flag leaves and grains after anthesis under the different treatments. It was found that, under the same phosphorus application rate, compared with S0 treatment, the SPAD value and Pn, the contents of soluble sugar and sucrose, and the activities of sucrose synthetase and sucrose phosphate synthetase in flag leaves and grains were all significantly increased in the S1 treatment. Finally, the dry matter accumulation and grain yield were, respectively, increased by 9.25%-14.60% and 2.17%-6.31%. Under the same straw return, with increase in phosphorus application rate, the SPAD value, Pn of flag leaves, the contents of soluble sugar and sucrose and the activities of sucrose synthetase and sucrose phosphate synthetase in flag leaves and grain, and dry matter accumulation showed a trend of initial increase and then decrease. Considering the interaction of straw return and P fertilizer application rate, the S1P3 combination had the best performance for SPAD value, Pn, the contents of soluble sugar and sucrose and the activities of sucrose synthetase and sucrose phosphate synthetase in flag leaves and grain, and thus resulted in the highest grain yield, which was 35.70% greater than that in S0P0. Therefore, the S1P3 treatment with full straw return in combination with a P fertilizer application rate of 150 kg·ha-1 was the most suitable planting configuration under the present experimental conditions and should be promoted as optimal in rainfed wheat production in western Henan province.

    Enzyme extraction method and antioxidant activity of polysaccharides from red clover
    Zhao-ben QI, Xiao-yan REN, Yi-tong LI, Jin-yun MA, Quan LIU
    2024, 33(6):  105-115.  DOI: 10.11686/cyxb2023260
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    Red clover (Trifolium pratense) is a resource-rich perennial leguminous forage species that is cultivated worldwide. Its polysaccharide components have good hypoglycemic and antioxidant properties, and have potential applications in the development of new, high-value products. The polysaccharides can be extracted using enzymes, and milder extraction conditions are conducive to retaining their beneficial properties. In this study, the effects of enzymatic hydrolysis time, enzyme dosage, enzyme ratio, and enzymatic hydrolysis temperature on the extraction of red clover polysaccharides were investigated. The extraction conditions were optimized and screened by orthogonal experiments. The results showed that the best enzyme extraction method was as follows: enzymatic hydrolysis time of 90 min, enzyme dosage of 1.0%, enzymatic hydrolysis temperature of 60℃, and enzyme ratio papain∶cellulase∶pectinase of 7∶2∶2. The crude polysaccharide content in red clover was 8.85%. We compared the enzyme extraction method with two other methods; ultrasonic extraction and hot water extraction. Red clover polysaccharides were extracted using all three methods, and their physical and chemical properties and antioxidant activity were compared. All three polysaccharide extracts showed antioxidant activity, with stronger abilities to scavenge O2·- free radicals than that of the positive control, vitamin C. The molecular weight ranges of polysaccharide samples prepared using the different extraction methods were significantly different, and the enzyme extract and hot water extract were more concentrated than the ultrasonic extract. The three polysaccharide samples were composed of eight types of monosaccharides, among which glucose, galactose, and arabinose had relatively high molar ratios, accounting for approximately 3/4 of the monosaccharide components. The proportions of monosaccharides varied widely among the samples, and the molar percentage of glucose was highest in the enzyme-extracted polysaccharides (1.6 times that in hot water-extracted polysaccharides and 1.4 times that in ultrasonically extracted polysaccharides).

    Wild legume forage resources in the Dabie Mountainous Area of eastern Hubei
    Jun-bo XIONG, He-shan ZHANG, Hong TIAN, Jiao-yun LU, Xin-jiang Wu, Yang LIU
    2024, 33(6):  116-125.  DOI: 10.11686/cyxb2023262
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    To effectively develop and utilize wild plant resources, the wild legume forage resources distributed in the Dabie Mountainous Area of eastern Hubei were investigated and quantified from 2017 to 2021. These investigations included evaluations of the resource status, genus and species composition of the flora, and the life form and feed value of wild legume forage plants in this region. The results showed that the legume forage resources in Dabie Mountain Area of eastern Hubei belonged to 34 genera and 77 species, among which a few species and a few monospecific genera were dominant, accounting for 91% of the total resources. These resources included six endemic species in China, two rare and endangered species in China, and 23 wild grasses and wild relatives of cultivated pastures. As a proportion of all the wild legume resources, 47% were temperate species and 38% were tropical species. The main life forms were perennial herbs and shrubs, accounting for 70% of the total wild legume resources. Among the wild legume forage resources, 79% were of excellent feed quality, and the rest were of medium feed quality or better.

    Genome-wide identification and bioinformatics analysis of CBF/DREB1 transcription factors in wild rice
    Chen-min HUO, Min YUAN, Bao-wen ZHANG, Rui-ju WANG
    2024, 33(6):  126-144.  DOI: 10.11686/cyxb2023251
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    Low temperature and cold damage are important factors limiting the regional distribution and yield of cultivated rice, and this seriously affects food security in China. The C-repeat binding factor cold-regulated gene (CBF-COR) pathway with CBF/DREB1 transcription factors as the core component is an important signal transduction pathway involved in the cold acclimation of rice. A total of 71 members of the CBF/DREB1 gene subfamily were identified based on whole genome sequencing results and hidden markov model (HMM) searches of nine species in the rice (Oryza) genus. All genes were intron-free and consisted of a single exon. Most of them encoded weakly acidic proteins. All putative DREB1 proteins had a grand average of hydropathicity (GRAVY) of <0. A phylogenetic analysis divided into three groups: Group I (DREB1A/1B/1H), Group Ⅱ(DREB1C/1E/1F/1G), and Group Ⅲ (DREB1D/1I/1J). The motif composition differed among the groups, although all of the putative proteins contained the characteristic AP2 domain and flanking sequence. An adaptive evolutionary analysis of japonica subspecies and other rice orthologuous gene pairs showed that the nonsynonymous nucleotide substitution rate/synonymous nucleotide substitution rate (Ka/Ks) values for the gene pairs OsDREB1A/ObDREB1AOsDREB1D/OnDREB1D,OsDREB1I/OsIDREB1I, and OsDREB1J/OsIDREB1J were greater than 1, indicating that they were under positive selection pressure. The types and numbers of promoter elements were similar in orthologs, but differed between paralogs. Real-time quantitative PCR analyses were conducted for 7-day-old seedlings of Nipponbare, 93-11 and Dongxiang wild rice after 2 hours of cold treatment at 4 ℃. The results showed that, during the early cold response, some DREB genes were activated (DREB1A/1B/1C/1E/1F/1G/1H) and others were inhibited (DREB1D/1I/1J). Among the cold-activated genes in Nipponbare, 93-11, and Dongxiang wild rice, DREB1B/1G/1H were the first and the fastest to respond to cold. All of these three genes had at least one copy of the CAMTA transcription factor binding element CM2 (CCGCGT) in their promoter regions. The transcript levels of OrDREB1B/1C/1E/1F/1G/1H in Dongxiang wild ricewere higher than those of their homologs in cultivated rice after 4 hours of cold treatment at 4℃. The results of this molecular evolutionary study on the CBF/DREB1 gene subfamily in nine species in the Oryza genus provide a preliminary basis for the mining and utilization of low-temperature resistance genes.

    Effect of harvest period and processing method on the quality and in vitro digestibility of native grass products
    Yi-fan WANG, Xing-liang ZHUO, Lei WANG, Hong-rui ZHANG, Xue CHEN, Fang-cai JI, Zhu YU
    2024, 33(6):  145-154.  DOI: 10.11686/cyxb2023248
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    This research explored the ideal harvesting time and feasibility of ensilage of native grass in the Hulun Buir region. Native grass was harvested on July 11 (T1 group), July 16 (T2 group), July 21 (T3 group), July 26 (T4 group), and July 31 (T5 group) in 2022. The harvested native grass was processed by natural drying, ensiling with fermentation, or artificial drying. The fermentation quality, nutritional quality, and in vitro digestibility of the products were evaluated. It was found that the fermentation quality of silage prepared from native grass harvested at the five different times was good. Compared with the other groups, the T3 and T4 groups of native grass silage had the highest levels of lactic acid (LA) and acetic acid (AA), and lower pH values and NH3-N/TN. Compared with their corresponding naturally dried groups, the silage made from the T2, T3, T4, and T5 groups showed significantly higher crude protein (CP) content (P<0.05), significantly lower soluble water-soluble carbohydrate (P<0.05), and no significant difference in neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents (P>0.05). The in vitro digestibility of dry matter (IVDMD) and crude protein (IVCPD) of native grass significantly increased with later harvest times (P<0.05). In the silage fermentation, natural drying, and artificial drying treatments, the T4 group of harvested native grass had the highest CP content, lower NDF and ADF contents, and higher IVDMD and IVCPD. The nutritional quality and in vitro digestibility of native grass silage were higher than those of naturally dried and artificially dried native grass. The quality of silage prepared from native grass harvested on July 26th was the best. Therefore, it is recommended to harvest native grass for ensiling in mid- to late July.

    Anatomical and histochemical features of amphibious Cyperus rotundus
    Hai ZHENG, Ying WANG, Juan XU, Ting-ting ZHU, Ge-ge QIN, Cun-yu ZHOU, Chao-dong YANG, De-bao TAN, Xia ZHANG, Hong-bo WEI
    2024, 33(6):  155-164.  DOI: 10.11686/cyxb2023266
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    This research studied the vegetative organs of Cyperus rotundus distributed in the drawdown zone of the Three Gorges Reservoir and Jianghan Floodplain along the Yangtze River. In this study samples were sectioned under an anatomical microscope and we used histochemical, optical and fluorescence microscopy methods to observe, photograph and record the anatomical structure and histochemical characteristics of leaves, erect stems, rhizomes, tubers and adventitious roots of C. rotundus adapted to amphibious environment. It was found that: 1) C.s rotundus has typical characteristics for adaption to an amphibious environment, including tissue air cavities and an apoplastic barrier in roots. Air cavities were observed in the lysed aerenchyma in the adventitious roots, rhizomes and leaves and schizogenous aerenchyma in erect stems. The apoplastic barrier included endodermis, exodermis, suberized stele and vascular bundle sheaths. 2) The adventitious roots of C. rotundus have a suberized stele, while stems have suberized vascular bundles, and the Kranz anatomy of leaves and erect stems consists of three layers: inner sheaths, mestome sheaths and parenchymatous sheaths. 3) The apoplastic barrier of C. rotundus may help to retain oxygen and control the exchange of water and ions between the environment and plant tissues under submersion. The air cavities are conducive to the preservation and transport of oxygen for aerobic metabolism. The adaptation of C. rotundus to submerged terrestrial environments indicates that it is likely to be an important species for ecological restoration of the Yangtze River.

    Identification of seed vigor and evaluation of seed storability in different varieties of oat
    Zhao ZHANG, Ying-ying FU, Hao-wen SUN, Feng-xue SUN, Hui-fang YAN
    2024, 33(6):  165-174.  DOI: 10.11686/cyxb2023271
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    This research explored the differences in seed vigor and storability among different varieties of oat and screened germplasm with high vigor and strong storability. We studied seeds of eight varieties of oat and simulated seed storage by the artificial aging method of seed storage at 45 °C and 100% relative humidity. Responses of 11 indicators related to seed germination and seedling growth were compared for 0 day (i.e. non-aged, control) and after four days. The storability of the tested seeds was evaluated using multivariate methods, including principal component analysis, membership function analysis, comprehensive evaluation value determination (D value) and cluster analysis, to generate an aging tolerance coefficient, and a comprehensive aging tolerance coefficient. This provides a theoretical reference data set for the selection of oat germplasm with high vigor and strong storability and the cultivation of new varieties. It was found that germination energy, germination percentage, germination index, seed vigor index, seedling percentage and seedling vigor index all showed a decline trend under artificial aging, while mean germination time was extended. In principal component analysis, the 11 individual measurement indicators were transformed into 3 independent multivariate scores. Data for the comprehensive evaluation value (D value) of storability and cluster analysis, showed that the 8 oat varieties can be classified into four types: Namely those with strong storability, medium storability, weak storability and extremely weak storability. Variety “Baler Ⅱ” was identified as having strong storability and “Haywire” as having extremely weak storability. This study identifies oat varieties with high vigor and strong storability, and provides a basis for the mining of key candidate genes and the creation and utilization of new germplasm.

    Effects of cerium nitrate and abscisic acid treatment on alfalfa seed germination and seedling physiological characteristics
    Jin-zhu GAO, Dong-hao ZHAO, Le GAO, Xi-hao SU, Xue-qing HE
    2024, 33(6):  175-186.  DOI: 10.11686/cyxb2023281
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    This research studied the effect of rare earth cerium (Ce) and exogenous abscisic acid (ABA) treatment on seed germination and seedling physiological characteristics of alfalfa. Cerium nitrate, ABA, and cerium nitrate and ABA (1∶1) mixed solutions with concentrations of 5, 10, and 20 μmol·L-1 were used to treat alfalfa seeds to conduct germination tests and explore the impact on seed germination, growth, seedling chlorophyll content, antioxidant enzyme activity, and contents of osmoregulation substances in seedlings. It was found that cerium nitrate treatment at 5 and 10 μmol·L-1 significantly stimulated the germination index of alfalfa seeds (but not germination percentage) and all three concentrations of cerium nitrate (5-20 μmol·L-1) increased seedling root diameter. Meanwhile, ABA at all three concentrations (5-20 μmol·L-1) inhibited seed germination index and root and hypocotyl length. The mixed treatment of cerium nitrate+ABA had similar effects to those of ABA alone except that ABA neutralized the effect of cerium nitrate on root diameter and seedling suppression effects of ABA tended to be exacerbated by cerium nitrate at the higher concentration of 20 μmol·L-1. ABA decreased seedling chlorophyll content but the ABA effect was partially neutralized by addition of cerium nitrate. ABA generally did not change the antioxidant enzyme activity of seedling leaves, but the mixed treatment of Ce and ABA at the higher concentration of 20 μmol·L-1 resulted in significantly increased antioxidant enzyme activity of alfalfa seedlings. Therefore, cerium nitrate treatment has a partial positive effect on the germination and growth of alfalfa; When cerium nitrate is mixed with ABA, ABA plays a dominant role in regulating the germination and seedling growth processes as well as physiological characteristics of alfalfa. The high antioxidant activity of seedlings treated with cerium nitrate+ABA seems to indicate that the seedlings are under stress. This study provides a reference for the effect of rare earth cerium and ABA on the germination period of alfalfa.

    Progress in research on breeding methods to produce new, high-quality forage varieties
    Wen-wen QI, Hong-yuan MA, Ya-xiao LI, Yan DU, Meng-dan SUN, Hai-tao WU
    2024, 33(6):  187-202.  DOI: 10.11686/cyxb2023259
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    Forage is the best natural feed for herbivorous livestock. Therefore, it the basis and guarantee for the development of the herbivorous animal husbandry industry, and an important food resource as part of an all-encompassing approach to food. The breeding of new forage varieties is essential for the sustainable development of grass and animal husbandry industries. Forage plays important roles in promoting the stable production and supply of animal products. With the increasing demand for new high-quality forage varieties and the improvement of breeding techniques, forage breeding technology has entered the molecular era, and great breakthroughs have been made in the cultivation of new high-quality forage varieties. In this review, we systematically summarize the forage breeding techniques used in China and abroad in the past 100 years, including conventional breeding techniques (domestication breeding, cross breeding, mutation breeding, and ploidy breeding) and genetic engineering breeding techniques (transgenic and molecular design breeding). We also discuss the gene editing methods developed in recent years. We describe the achievements made using various breeding techniques, as well as their problems. We also outline the following prospects for future research: 1) In-depth exploration of natural forage resources and strengthening of the collection and utilization of germplasm resources. 2) Advancing the objectives for the breeding of forage species to meet the demands of farmers and livestock producers, paying attention to the improvement of forage quality, the production of disease-resistant and stress-tolerant varieties, and the development of the “productional-living-ecological” function of forage. 3) Combining conventional and modern breeding methods to move past the bottleneck of forage breeding and strengthen the breeding of high-quality forage species, especially Leymus chinensis and Medicago sativa. The aims of this review are to promote the new era of biological breeding in China and to provide a basis for the innovation of forage germplasm resources and the selection of new, high-quality forage varieties. The overall aims in this field of research are to provide new technologies for the establishment of high-quality and high-productive cultivated grassland, and to meet the growing demand for forage to feed farmed animals in China.

    The mechanism of PGPR regulating plant response to abiotic stress
    Guo-qiang WU, Zu-long YU, Ming WEI
    2024, 33(6):  203-218.  DOI: 10.11686/cyxb2023276
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    Salt alkali, drought and other abiotic stresses are important environmental factors that limit plant growth and yield. Plant growth promoting rhizobacteria (PGPR), as beneficial microorganisms colonizing plant roots, have been shown to have a capacity for use as biological agents, thereby harnessing their functions for human benefit. This methodology has advantages compared to traditional agricultural chemicals, including low cost, high efficiency, and environmental protection. PGPR have been documented to not only promote plant growth and crop yield, but also to significantly improve the tolerance of plants to abiotic stress. In this study, the definition and types of PGPR, their biological functions and their role in plant response to abiotic stress such as salinity, drought, high and low temperature, and heavy metals were reviewed, and future research directions were also explored. The results from this study provide a foundation for further research on PGPR mediated plant stress resistance and the development and application of these biological agents.

    Effects of dietary energy levels on testis development and the expression of related genes in sheep
    Pei-di ZHAO, Ke YANG, Yu-qi JIANG, Ke-pan HUANG, Ming-yu MA, Kai-dong LI, Hui XU, Wan-hong LI
    2024, 33(6):  219-226.  DOI: 10.11686/cyxb2023252
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    We investigated the effect of dietary energy levels on testicular development in sheep during the pre-puberty stage. Forty-five 3-month-old merino lambs with similar body weight [(24.27±0.63) kg] were randomly divided into three groups; namely high (10.5 MJ·kg-1), medium (10.1 MJ·kg-1) and low (9.7 MJ·kg-1) energy level groups. After a feeding period of 104 days, 10 lambs were randomly selected from each group for slaughter, and their testicular size, total antioxidant levels in testicular tissue, glutathione peroxidase activity, and testosterone levels were measured. The transcript levels of genes related to energy metabolism and steroid hormone synthesis were detected by RT-qPCR. The results indicated that the diets with different energy levels did not significantly affect body weight (51.73±0.56 kg, 50.69±0.55 kg and 51.51±1.03 kg for high, medium and low energy level groups, respectively), testicular weight (253.60±19.69 g, 274.60±14.17 g and 262.40±19.91 g, respectively), total antioxidant level (10.59±1.13 U·mg-1 prot, 8.60±1.00 U·mg-1 prot and 7.86±1.73 U·mg-1 prot, respectively), glutathione peroxidase activity (0.28±0.08 U·mg-1 prot, 0.14±0.03 U·mg-1 prot and 0.23±0.03 U·mg-1 prot, respectively), or testosterone level (0.52±0.07 ng·mg-1 prot, 0.35±0.05 ng·mg-1 prot and 0.44±0.06 ng·mg-1 prot, respectively) in testicular tissue (P>0.05). However, the transcript levels of the metabolism-related genes IGF-1R and IGF-1 and the steroidogenesis-related genes CYP19A1 and 3βHSD were significantly higher in the middle energy level group than in the other two groups (P<0.05). In summary, dietary energy levels potentially affect testicular metabolic activity in sheep. Feeding lambs with a middle energy level diet during the pre-puberty stage promoted the expression of metabolism- and steroidogenesis-related genes, but did not significantly affect testicular development.

    Identification and analysis of miRNAs in subcutaneous fat tissue of yaks
    Wei-qin DING, Yong-gang SUN, Yin-cang HAN, Ya-qian LIU, Sheng-wei JIN
    2024, 33(6):  227-235.  DOI: 10.11686/cyxb2023258
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    The aim of this work was to determine how different types of feeding affect fat deposition in yaks. Specifically, we aimed to identify differentially expressed microRNAs (miRNAs) in subcutaneous fat tissues of yaks raised under different feeding methods. High-throughput sequencing data were analyzed to identify the miRNAs present in the subcutaneous adipose tissues of yaks fed using different methods, and DESeq was used to identify the differentially expressed miRNAs among the different feeding methods. The target genes of the differentially expressed miRNAs were predicted using online tools. The treatment groups were F24, G18, and G24. G18 were naturally grazed 18-month-old yaks, G24 were naturally grazed 24-month-old yaks, and F24 were yaks that had been fattened for 6 months since G18. A total of 1158 miRNAs were identified in the subcutaneous fat tissue of nine yaks, including 731 known miRNAs and 427 newly identified miRNAs. There were 43 differentially expressed miRNAs (with 2436 predicted target genes) in the G18 vs F24 comparison; 68 differentially expressed miRNAs (with 3559 predicted target genes) in the G18 vsG24 comparison; and 31 differentially expressed miRNAs (with 1456 predicted target genes) in the F24 vsG24 comparison. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the predicted target genes of the differentially expressed miRNAs were enriched in fatty acid metabolism, fatty acid biosynthesis, fatty acid biooxidation, unsaturated fatty acid metabolism, and regulation of adipocyte differentiation. Therefore, supplemental feeding of yaks during the dry grass period leads to subcutaneous fat tissue deposition.