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    20 November 2024, Volume 33 Issue 11
    Risk assessment of invasive alien herbaceous plants in the Nishan-Yishan area
    Jing LIU, Yi-han YANG, Yong-qiang QIAN, Yu-feng CHEN, Dan LIU, Xiao-man XIE, Yue-han LIU, Xin LIU, Bo-kun ZOU
    2024, 33(11):  1-14.  DOI: 10.11686/cyxb2023501
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    Invasive alien plants pose a significant threat to biodiversity and ecosystem stability in Shandong Province. In this study, we conducted a field investigation and analyzed the vegetation composition characteristics and risk levels of alien invasive plant species, with an aim to provide a scientific basis for the prevention and control of invasions and for the management of alien species in Shandong Province. We established an invasive alien plant assessment system adapted to the ecological environmental characteristics of the Nishan-Yishan area. We used an analytic hierarchy process to assess the relative invasion risk of each species in the Nishan-Yishan area, and classified the species into four invasion risk grades. In total, 74 species of invasive herbaceous plants belonging to 23 families and 56 genera were identified in Nishan-Yishan. Asteraceae was the dominant family among the invasive species, with 14 genera and 20 species. Of the 74 species of invasive plants, 56 species were annual or biennial herbs, and 49 species originated from the Americas. In the risk assessment, nine species were classified as aggressive invaders, and these included eight genera and seven families. Conyza canadensis and Phytolacca americana had the highest invasion risk score, and were classified as extremely invasive. Additionally, 18 species were classified as locally invasive, and 19 species were classified as generally invasive. Twenty-eight species required further analysis — these species had a relatively light influence on ecosystem services and the economy. Qualitative and quantitative assessments were conducted on both the probability of invasion and potential harm caused by these alien plants to local ecosystems. Based on the invasion risks identified through this assessment, corresponding management practices can be effectively implemented to prevent invasions while safeguarding ecological security.

    Effects of plowing and plant residue mulching on soil nitrous oxide emissions in a black soil beach-type degraded grassland
    Ya-si FENG, Wen-ting JIANG, Yi-hong LIU, Yan WANG, Yuan LI, You-chao CHEN, Yan-jiang CAI
    2024, 33(11):  15-29.  DOI: 10.11686/cyxb2023485
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    Grassland restoration is the key measure to promote the restoration of the grassland ecosystem and achieve sustainable development. The aim of this work was to explore the effects of plowing and mulching with plant residues on soil nitrous oxide (N2O) emissions from an extremely degraded alpine grassland. The experiment was conducted in an alpine meadow in Hongyuan County, Sichuan, China, on the Eastern edge of the Qinghai-Tibetan Plateau. The experiment consisted of a control (CK) and three treatments; i.e., black soil beach-type degraded grassland with roots removed from the 0-20 cm soil layer and no plant residues (CK), plowed degraded grassland (PL), degraded grassland mulched with plant residues (MR), and plowed degraded grassland mulched with plant residues (PL+MR). We measured soil basic physical and chemical indicators, microbial biomass, extracellular enzyme activity, nitrification enzyme activity, denitrification enzyme activity, functional microbial gene abundance, and 28-day cumulative N2O emissions. The results showed that, compared with CK, PL significantly increased cumulative soil N2O emissions (by 44.2%). Soil N2O emissions were significantly lower in PL+MR (by 29.1%) than in PL. Compared with CK, PL significantly enhanced soil pH (by 2.6%), increased dissolved organic carbon (DOC) and microbial biomass carbon (MBC) concentrations (by 209.5% and 23.8%, respectively), increased activities of β-1,4-glucosidase (BG) and β-1,4-N-acetylglucosaminidase (NAG) (by 180.4% and 233.9%, respectively), and increased abundance of amoA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) (by 74.6% and 68.0%, respectively). Compared with CK, PL significantly decreased soil C/N (by 11.3%), decreased soil organic carbon (SOC) and microbial biomass nitrogen (MBN) concentrations (by 13.6% and 72.8%, respectively). Compared with PL, PL+MR significantly decreased soil DOC concentration (by 12.8%), significantly reduced BG, NAG, leucine aminopeptidase (LAP) and acid phosphatase (AP) activities (by 49.1%, 59.9%, 31.6%, and 25.0%, respectively), and decreased AOA-amoA, AOB-amoA, and nosZ I genes abundance (by 46.5%, 59.5%, and 23.1%, respectively). Compared with PL, PL+MR significantly increased MBN concentration (by 29.1%). Correlation analyses showed that cumulative soil N2O emissions were significantly positively correlated with the pH, DOC concentration, extracellular enzyme activity, and abundance of genes related to the nitrogen cycling (except nirS), and significantly negatively correlated with C/N, SOC and MBN concentrations. The abundance of AOA-amoA and AOB-amoA were the key factors affecting N2O emissions. In summary, plowing resulted in enhanced soil extracellular enzyme activity and amoA gene abundance, as well as increased SOC decomposition and consumption and N2O emissions. Mulching with plant residues effectively alleviated these negative effects, indicating that it is a feasible improvement measure.

    Initial effects of short-term warming on the productivity of alpine degraded grassland in northern Tibet during the restoration process
    Hao SHI, Cai-hong YANG, Fei XIA, Jun-qiang WANG, Wei WEI, Jing-long WANG, Yun-yin XUE, Shai-kun ZHENG, Hao-yang WU, Lin-ling RAN, Shuang YAN, Xiao-min JIANG
    2024, 33(11):  30-45.  DOI: 10.11686/cyxb2023484
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    Climate warming is expected to affect the productivity of alpine grassland in northern Tibet, and this may affect the grassland restoration process. The aim of this study, therefore, was to determine the effects of a short-term temperature increase on the productivity of alpine grassland during restoration. Natural restored grassland (NR), no-tillage replanted grassland (S), and tilled replanted grassland (TS), with native heavily degraded grassland as the control (CK), were subjected to a warming treatment using the open top chamber method. The changes in the plant community, biomass, and species diversity in response to this short-term temperature increase were determined. The results showed that: 1) Warming increased the surface soil temperature by an average of 1.34 ℃ and decreased the soil moisture content by an average of 4.18%. 2) After short-term warming, the plant community cover increased significantly (P<0.05) and the height of plant community decreased significantly (P<0.05); the biomass of vegetation in CK transferred to the underground plant parts, the biomass of vegetation in NR, S, and TS shifted to the aboveground plant parts; and the aboveground biomass decreased significantly in TS (P<0.05). 3) Short-term warming decreased the aboveground biomass and importance value of grasses (P<0.05) but increased the aboveground biomass and importance value of forbs (P<0.05). 4) In CK, warming significantly increased the species richness index, Shannon-Wiener index, and Simpson index by 17.65%, 18.54%, and 11.52%, respectively (P<0.05). In S, the warming treatment resulted in a significant decrease in the Pielou index (P<0.05). 5) After short-term warming, the above-ground biomass showed a highly significant negative correlation with the species diversity index (P<0.01); however, the below-ground biomass showed no significant correlation with the species diversity index or community characteristics. In conclusion, short-term warming had a certain positive effect on the recovery of the species diversity in native heavily degraded grassland in northern Tibet. Short-term warming restored the productivity of naturally restored grassland and no-tillage replanted grassland, but suppressed the growth and reduced the productivity of tilled replanted grassland.

    Effects of nitrogen fertilization on soil aggregate biological binding agents and stability in an alpine grassland
    Qing-hua TIAN, Dan LIU, Xiao-qin LIAO, Xiao-yan SONG, Lei HU, Chang-ting WANG
    2024, 33(11):  46-57.  DOI: 10.11686/cyxb2024055
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    In recent years, human activities and climate change have led to the deterioration of soil structure in alpine grasslands. Soil aggregates, as the fundamental structural units of soil, are mainly stabilized by the presence of biogenic substances. However, there is limited research on the relationship between soil aggregate characteristics and biogenic substances in grassland soils. In order to clarify the differences in soil aggregate biogenic substances and stability under different nitrogen application levels, the alpine grasslands of Hongyuan were studied. A randomized block design with seven nitrogen rates was employed, namely 0 (CK), 10, 20, 30, 40, 50, and 60 g·m-2 urea (46.7% N). The differences in soil aggregate composition and stability in relation to the content of biogenic substances were measured and analyzed. The results showed that: 1) Soil aggregates in alpine grasslands were mainly in the 2-4 mm size class, which accounted for 34.22% of the total. Different nitrogen rates significantly influenced soil nutrient content, aggregate composition, and stability (P<0.05). 2) The content of biogenic substances [soil spore density, hyphal density, easily extractable glomalin (EEG), difficult-to-extract glomalin (DEG), and total glomalin (TG)] varied significantly under different nitrogen rates (P<0.05). Specifically, the highest spore density value of 72.33 ind·g-1 occurred in N10, the lowest hyphal density value of 0.48 m·g-1 occurred in N60, and the highest TG value of 6.84 mg·g-1 occurred in N60. 3) Correlation analysis revealed a significant positive correlation between hyphal density and the percentage weight of 2-4 mm soil aggregates, and a highly significant negative correlation with the percentage weight of 0.25-1.00 mm soil aggregates. The percentage weight of 0.25-1.00 mm soil aggregates was significantly positively correlated with glomalin. Stepwise regression analysis identified EEG as an important factor influencing changes in the percentage weight of soil aggregates <0.25 mm (R2=0.26). Path analysis demonstrated that hyphal density had the largest direct positive effects on mean weight diameter (MWD) and geometric mean diameter (GMD), with values of 0.79 and 0.78 respectively. The study results indicate that nitrogen application reduces the stability of soil aggregates in alpine grasslands, with hyphal density and EEG having the most significant effects on soil aggregate composition and stability (P<0.05).

    Evaluation of soil quality change over time when retiring cultivated farmland on gravel-sand mulched fields in central Ningxia
    Si-yao WANG, Kai-yang QIU, Jian-yu WANG
    2024, 33(11):  58-68.  DOI: 10.11686/cyxb2024017
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    Information on the change over time in soil properties when retiring cultivated land in Ningxia’s gravel-sand mulched field areas is highly relevant for advancing ecological conservation and constructing high-quality development pilot zones in the Yellow River Basin. Focusing on the gravel-sand mulched fields in the Xiangshan area of Zhongwei City, Ningxia, a spatial sequence method was employed instead of a time series approach. Gravel-sand mulched fields with consistent terrain and soil type are selected for study of the variations in soil physicochemical properties and enzyme activities 2, 5, 10, 15, 20, 30 and 100 years after retirement from cultivation. The soil quality for these fields with differing retirement dates was evaluated using a combination of subordinate function and factor analysis methods. Results indicate: 1) Soil potassium content decreased overall after five years of retirement. Soil nitrate-nitrogen content peaked at two years of retirement, with no significant differences between other periods. Soil total phosphorus, available phosphorus, and available potassium content fluctuated with years of retirement, peaking at 15, 2, and 15 years of retiring, respectively. 2) Soil porosity initially decreased and then increased with increased years of retirement. Field capacity and soil moisture content were highest at two years after retirement, sharply decreased at five years, and significantly increased with years of retirement, thereafter. Soil water content tended to be stable after 20 years of returning farmland. 3) Soil urease and invertase activities initially decreased and then increased with increasing years of retirement, both peaking at 30 years of retirement. 4) The multi-trait scores for soil quality under different retirement periods were ranked: 2 years>30 years>100 years>15 years>20 years>10 years>5 years. Soil quality evaluation scores peaked at 2 years of retirement. We interpreted this to indicate that after an initial recovery evident at 2 years, a decline is seen at 5 years, and then soil quality improves with time in retirement.

    Effects of Chloris virgata and arbuscular mycorrhizal fungi on the growth of Leymus chinensis under alkali stress
    Xiao-yu LU, Ya-jie LIU, Cai-xia BAI, Jin-hua LI, Zi-he WANG, Chun-xue YANG
    2024, 33(11):  69-83.  DOI: 10.11686/cyxb2023475
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    Leymus chinensis and Chloris virgata are common plants that are able to tolerate the barren, saline-alkali soils of the Songnen grassland. This research investigated the effects of C. virgata and arbuscular mycorrhizal (AM) fungi on the alkali tolerance of L. chinensis. A pot experiment was conducted with L. chinensis as the host plant, C. virgata as the companion species, and AM fungal communities derived from the rhizosphere of L. chinensis from Songnen saline-alkali grassland as the inoculant. The experiment therefore had three variables: AM fungi, a companion plant, and alkali stress. The mycorrhizal infection rate, growth index, leaf chlorophyll content, malondialdehyde (MDA) content, antioxidant enzyme activity, and osmoregulatory substance content of L. chinensis were measured to analyze the effects of the companion plant and AM fungal inoculation on the physiology and growth of L. chinensis under alkali stress. The main results were as follows: 1) The AM fungi effectively colonized the roots of L. chinensis under alkali stress, and C. virgata inhibited this symbiosis. 2) Compared with uninoculated L. chinensis, plants of L. chinensis inoculated with AM fungi showed a significantly reduced MDA content, and increased biomass, chlorophyll content, antioxidant enzyme activities, and osmoregulatory substance contents, which resulted in enhanced alkali tolerance. 3) The biomass of L. chinensis was lower in the control than in the treatment groups. Compared with L. chinensis in the control group, L. chinensis in the alkali treatment group showed higher contents of osmoregulatory substances, increased enzyme activities, and improved growth. 4) The interaction between C. virgata and AM fungi was associated with increased leaf width, saturation fresh weight, chlorophyll content, soluble sugar content, and soluble protein content, and enhanced activities of peroxidase and catalasein L. chinensis. 5) A membership function analysis showed that AM fungi can interact with C. virgata and improve the tolerance of L. chinensis in alkali habits. These results provide a theoretical basis for evaluating the effects of AM fungi and C. virgata as a companion plant on the growth of L. chinensis under alkali stress. These findings also provide important data to assist development of methodologies for the ecological restoration of Songnen saline-alkali grassland by introduction of appropriate companion plants and exploitation of mycorrhizal symbioses.

    Phylogeography of Potentilla anserina distributed across the Qinghai-Tibet Plateau
    Shi-jun BAI, Jun-qiao LI, Xin LIU, Bo-wen LYU
    2024, 33(11):  84-105.  DOI: 10.11686/cyxb2023492
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    The uplift of the Qinghai-Tibetan Plateau and the cyclic fluctuation of the climate during the Quaternary Ice Age have greatly affected the distribution and genetic structure of plants and animals in this area and neighboring areas. Juema is an endemic plant of the Tibetan Plateau with high nutritional, medicinal, and ecological values. The taxonomic relationship and distribution of juema and Potentilla anserina are controversial in academia. Therefore, we conducted molecular genealogical and geographical analyses to explore the formation of genetic diversity in juema and to infer its migratory evolutionary history. We collected 810 individual plants from 30 populations and determined the sequences of their chloroplast trnL-trnF and nuclear internal transcribed spacer (ITS) regions. The data were analyzed to reveal the distribution of genetic variation within and among populations, and combined population genetics and phylogenetic analyses were conducted to determine the correlation between the genetic structure of the species and historical events. The aim of these analyses was to determine the evolutionary history of the species and the reasons for the current distribution pattern of different populations within the species. The main results were as follows: 1) Juema shows a high level of genetic diversity. Forty haplotypesof the cpDNA trnL-trnF fragment were detected, including 16 shared haplotypes, accounting for 40% of the total, and 24 population-specific haplotypes, accounting for 60% of the total (genetic diversity h=0.7078, haplotype diversity Hd=0.8217, nucleotide diversity π=0.010641, total genetic diversity HT=0.849). For the ITS region, 128 haplotypes were detected, including 42 shared haplotypes, accounting for 32.8% of the total, and 86 population-specific haplotypes, accounting for 67.2% of the total (h=0.7633, Hd=0.8168, π=0.003584, and HT=0.844). 2) Genetic diversity is greater within than among populations. Sequence analyses revealed high genetic diversity both within and among populations (cpDNA trnL-trnF: HT=0.849, gene diversity within populations HS=0.640; nrDNA ITS: HT=0.844, HS=0.763; HT>HS for both sequences). The juema populations were divided into three groups: The Qinghai Plateau group, the Hengduan Mountains group, and the South Tibetan Valley group. The genetic variation of juema has mainly originated from within populations. 3) Juema populations show a distinct genealogical geographic structure. We conducted genetic polymorphism and geographic distribution pattern analyses based on the cpDNA trnL-trnF and nrDNA ITS data. For cpDNA trnL-trnFthe genetic differentiation coefficients were GST=0.246 and NST=0.417; for nrDNA ITS, GST=0.096 and NST=0.522. For both sequences, NST was significantly larger than GSTP<0.001). The results were highly consistent between the two methods, and indicated that a significant spectral geographic structure exists in all population haplotypes of juema. Analysis of molecular variance (AMOVA) showed that the majority of genetic variation (59.69%) is within populations, with a high level of differentiation among populations (FST=0.40313). 4) Both shared and endemic haplotypes are derived from ancient haplotypes. Centrally linked reticulation maps based on cpDNA trnL-trnF and nrDNA sequences showed a stellate structure with the shared haplotypes M4 and H9 at the center, and the shared haplotypes M1 and M3, and H2, H10, H11, and H12 at the trunk positions. The remaining shared and unique haplotypes were derived from these ancient haplotypes, and the results were consistent. 5) There has been a major expansion in the size and range of the plant population. When cpDNA trnL-trnF and nrDNA sequences were subjected to a manifold analysis, the former showed a single-peak curve in the manifold distribution, indicating that a large-scale expansion of the size and range of the population has occurred recently; and the latter showed a double-peaked curve, reflecting a high degree of complexity in its genetic genealogy. The results of Tajima’s D, Fu and Li’s D, and Fu and Li’s F tests were all negative and significant, and the statistical tests of sum of squares deviations and Harpending’s raggedness parameters were not significant, suggesting the possibility of recent expansion of juema populations. 6) Juema exists in three Ice Age refuges, i.e., the Eastern Himalaya region, the southeastern edge of the Tibetan Plateau, and the Hengduan Mountains region.

    Identification of the BZR gene family in alfalfa and analysis of its transcriptional responses to abiotic stress
    Ze-bin LI, Yong-zheng QIU, Yan-jie LIU, Jin-qiu YU, Bai-ji WANG, Qian-ning LIU, Yue WANG, Guo-wen CUI
    2024, 33(11):  106-122.  DOI: 10.11686/cyxb2023490
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    Brassinosteroids (BRs) are a class of steroid hormones that influence various developmental and physiological processes in plants. Members of the BRASSINAZOLE-RESISTANT (BZR) transcription factor family are positive regulators of brassinosteroid biosynthesis. The BZR gene family plays a crucial role in regulating plant growth and development, including various aspects of stress resistance. However, the BZR gene family in alfalfa (Medicago sativa), which is known as the ‘king of forages’, has not been thoroughly studied. In this study, we identified 14 BZR genes in alfalfa, and used bioinformatics tools to analyze the physicochemical properties and secondary and tertiary structures of their encoded proteins. The chromosomal locations, phylogenetic relationships, structures, conserved domains, promoter cis-acting elements, gene family interactions, and transcript levels of the 14 genes in six different tissues of alfalfa, as well as their transcriptional responses to abiotic stresses, were also determined. We found that all 14 BZR genes were expressed in various tissues of alfalfa, with nine genes responding to cold stress, seven responding to salt stress, and seven genes responding drought stress. Notably, three BZR genes (MsBZR05MsBZR06and MsBZR12) responded to all three types of stress. The results of this study highlight candidate genes for further research on the BZR gene family.

    Heterologous expression of miR397-5p from Medicago sativa cv. ‘Pianguan’ improves the drought tolerance of tobacco
    Yu-xin WANG, Jia-li TAO, Hui-sen ZHU, Tao XU, Yi-fei ZHANG, Hui-fang CEN
    2024, 33(11):  123-134.  DOI: 10.11686/cyxb2023498
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    Medicago sativa cv.‘Pianguan’ is a well-regarded local germplasm in Shanxi Province, known for its strong stress resistance, adaptability, and resistance to barren soils. It is crucial to identify genes related to drought resistance in M. sativa cv. ‘Pianguan’, so that they can be utilized for the genetic enhancement of alfalfa, thereby facilitating the development of new germplasm. In this study, miR397-5p was identified as a potential microRNA responsive to drought stress in M. sativa cv. ‘Pianguan’ through miRNA sequencing analysis. The responsiveness of miR397-5p from M. sativa cv. ‘Pianguan’ to drought stress was validated in transgenic tobacco, and its potential downstream target, and its effects, were identified. After drought stress, miR397-5p-overexpressing tobacco showed elevated levels of osmotic regulators, including soluble sugars, soluble proteins, and proline. Additionally, the activities of the antioxidant enzymes catalase, superoxide dismutase, and peroxidase were increased, while the malondialdehyde content was decreased. Under drought stress, miR397-5p decreased the expression of its target gene LAC4. In miR397-5p-overexpressing tobacco, most genes encoding key enzymes in the lignin biosynthesis pathway (PAL4CLCCRCADHCTF5H, and PAOshowed decreased transcript levels, while genes such as C4HCCoAOMTCOMTand others exhibited increased transcript levels. Additionally, the lignin content was decreased in miR397-5p-overexpressing tobacco. The transcript levels of most of these genes and lignin content exhibited consistent trends.It was inferred that M. sativa cv. ‘Pianguan’ may regulate lignin accumulation in response to drought stress through miR397-5p-LAC4, but the specific underlying mechanism needs to be further investigated. Further research is required to clarify the mechanism of this response. The results of this study may provide a genetic resource for the genetic improvement of alfalfa, which is potentially valuable for the creation of excellent new germplasm.

    Abiotic stress priming affects the responses of maize (Zea mays) plants to cadmium stress
    Xiao-xiao LI, Pan ZHANG, Yuan LU, Ting LI, Na ZHAO, Jia-wen WU
    2024, 33(11):  135-148.  DOI: 10.11686/cyxb2023477
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    Cadmium (Cd) pollution in arable land in China is becoming an increasingly serious problem. The aim of this study was to elucidate how various abiotic stresses prime the responses of maize (Zea mays) to Cd stress. On the one hand, abiotic stress priming that reduces Cd accumulation can be used to safeguard the quality of maize for consumption; on the other hand, abiotic stress priming that increases Cd accumulation in maize can be used to enhance phytoremediation efficiency when maize plants are grown in Cd-polluted soil. Maize seedlings were subjected to priming treatments for a short time. Drought, salt, and iron deficiency were used as cross-stress priming treatments, and a low dose of Cd was used as the same stress priming treatment. After the various stress treatments, the maize seedlings were subjected to Cd stress, and then their biomass, root morphology, tolerance index, Cd concentration, Cd accumulation, subcellular Cd distribution, and antioxidant defense capacity were determined. The application of iron deficiency, salt stress, and drought as stress priming treatments resulted in a reduction in the dry weight of maize roots, stems, and leaves. However, priming with these abiotic stresses significantly enhanced the tolerance indices of maize under Cd stress. Specifically, drought stress priming led to a decrease in the Cd concentration and accumulation in the roots, stems, and leaves of maize plants. Additionally, it promoted the localization of Cd in vacuoles. By contrast, priming with a low dosage of Cd enhanced Cd concentrations in roots and leaves and Cd accumulation in leaves, but did not alter the subcellular distribution of Cd. Priming with abiotic stresses did not significantly change the activities of superoxide dismutase, peroxidase, and catalase in the leaves and roots of maize plants under Cd stress, suggesting that abiotic stress priming does not stimulate the activities of antioxidant enzymes during the response to Cd stress in maize. Correlation analyses showed that total root length was closely correlated with Cd accumulation in maize. The total root length was affected by abiotic stress priming. Moreover, the Cd concentration in the leaf cell walls was positively correlated with Cd accumulation in maize. In contrast, the Cd concentration in vacuoles of root cells was negatively correlated with Cd accumulation in maize. Our results show that abiotic stress priming can significantly promote the Cd tolerance of maize, and that drought priming can decrease Cd accumulation in maize.

    The phosphorus effect of applying phosphate rock powder during the green manure season in red soil of Jiangxi Province
    Shuai-lei LYU, Dan-na CHANG, Guo-peng ZHOU, Rui LIU, Xin ZHAO, Jia LIU, Chang-xu XU, Wei-dong CAO
    2024, 33(11):  149-160.  DOI: 10.11686/cyxb2024008
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    Rotation of rice and green manure crops is a common practice in red soil rice fields in southern China. This study investigated the effect of green manure on activating phosphate rock powder and its impact on the yield and phosphorus absorption of subsequent rice crops, to test the efficacy of using phosphate rock powder to replace phosphate fertilizer in red paddy soil. Pot experiments comprising two factors: green manure cultivars and rate of phosphate rock powder, were conducted in Nanchang city, Jiangxi Province. The green manure crops tested were Chinese milk vetch (Astragalus sinicus) (MV), rape (Brassica napus) (RA), and ryegrass (Lolium multiflorum) (RY) with a winter fallow (WF) as control. The phosphate rock powder application rates were 0 (P0), 0.74 (P1), and 1.48 g·kg-1 (P2). The dry weight of green manure during flowering, phosphorus absorption, the soil phosphorus pool, and phosphatase activity were measured, as well as the yield and phosphorus absorption of early and late rice. The results showed that the average aboveground dry matter weights of MVP2, RAP2, and RYP2 treatments were 18.31, 18.07, and 25.33 g·pot-1, respectively. These values were increases of 42.0%, 42.7%, and 38.6%, respectively, compared to the P0 treatment. The average phosphorus absorption amounts were 49.10, 60.83, and 36.13 mg·pot-1, respectively, representing increases of 40.5%, 86.7%, and 45.5%, respectively, compared to the P0 treatment. Compared to the P0 treatment, applying phosphate rock powder increased soil total phosphorus, microbial phosphorus content, and stable phosphorus pool percentage by 13.4%-34.2%, 23.0%- 93.2%, and 4.0%-10.6% respectively. Compared to WF treatment, the active phosphorus pool ratio in green manure treatments increased by 0.4%-1.0%, while the moderately active phosphorus pool ratio decreased by 0.6%-2.9%. The average grain yields of early and late rice were 26.33-45.87 g·pot-1 and 39.17-49.04 g·pot-1 respectively. Compared to the P0 treatment, the late rice grain yield increased by 8.5% and 11.8% in the MVP1 and MVP2 treatments, respectively, and the phosphorus absorption of early rice increased by 34.4% and 23.3% in the MVP1 and MVP2 treatments, respectively. The RAP1 treatment increased the late rice grain yield by 9.1%. The phosphorus absorption of early rice increased by 20.0% in the RYP1 treatment. Compared with the WF treatment, the utilization rates of phosphate rock powder in the early rice season for MVP1, RAP1, and RYP1 treatments increased by 2.2%, 1.5%, and 2.3%, respectively. For the MVP2 treatment, there was an increase of 1.9%. Additionally, the utilization rate of phosphate rock powder for MVP1 treatment in the late rice season increased by 2.6%. In conclusion, applying phosphate rock powder increased the biomass and phosphorus absorption of green manure, with better solubilization effects observed in Chinese milk vetch and rape than ryegrass. Using Chinese milk vetch as the green manure crop resulted in higher yield, phosphorus absorption, and phosphate rock powder utilization efficiency in second-crop rice than rape and ryegrass treatments.

    A multi-trait evaluation of phosphorus efficiency of 38 forage oat cultivars at the seedling stage
    Jing-bo YU, Hui-li ZHANG, Jin LI, Hao GUAN, Qing-ping ZHOU, Shi-yong CHEN
    2024, 33(11):  161-171.  DOI: 10.11686/cyxb2024065
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    This research investigated the response of forage oats to low phosphorus levels to identify phosphorus-efficient cultivars. A study was conducted on 38 forage oat varieties at the seedling stage, and 15 traits were measured and analyzed, including morphological and root traits, under low phosphorus (0.02 mmol·L-1 KH2PO4, LP) and normal phosphorus (1 mmol·L-1 KH2PO4, NP) treatments, using a hydroponic system. Phosphorus efficiency types were determined using principal component analysis, correlation analysis, multiple regression analysis, membership function analysis and cluster analysis. The results indicated that under low phosphorus stress, certain traits like root: shoot ratio, total root length, root tip number, and phosphorus use efficiency increased significantly (P<0.05), while plant height, shoot dry weight, root dry weight, total dry weight, root diameter, phosphorus content, and phosphorus accumulation decreased significantly (P<0.05). Under the low-phosphorus treatment, the coefficients of variation of the root-related indexes including the number of root tips, root forks, and root∶shoot ratio were all higher than in the normal phosphorus treatment, which were 49.24%, 55.24% and 46.11%, respectively. A correlation analysis was carried out between the integrated low phosphorus tolerance value (D) and the low phosphorus stress tolerance indices for the 15 traits using membership function analysis, and the low phosphorus tolerance coefficients of shoot dry weight, root dry weight, total dry weight, total root length, root surface area, root volume, root projected area, root tip, root fork, and phosphorus use efficiency showed a significant correlation with the D value. Using multiple regression analysis, seven indicators for the identification of low phosphorus tolerance were evaluated, and a regression equation for the D value was established. Finally, the cluster analysis divided the 38 forage oats into three categories: high-, medium-, and low-efficiency of phosphorus utilization. Of the tested varieties, three were phosphorus-efficient, namely Youmu No. 1, Kona and Sweet oat No. 1.

    Determination of optimal input levels of nitrogen, phosphorus and potassium for high yield and quality of hydroponic barley forage based on response surface methodology
    Ming-hui HOU, Yan-liang SUN, Kai-xin YANG, Jun-cang QI, Qian-bing ZHANG
    2024, 33(11):  172-185.  DOI: 10.11686/cyxb2024013
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    By analyzing the effects of different levels of nitrogen, phosphorus and potassium on the fresh grass yield and crude protein of barley forage, the optimal levels of nitrogen, phosphorus and potassium suitable for hydroponic cultivation of barley forage were identified, with a view to providing a theoretical basis and data reference for the optimal use of this resource. The range of values of nitrogen addition, phosphorus addition and potassium addition were determined by a single-factor experiment, a quadratic polynomial regression model was constructed by the response surface optimization method, and different levels of nitrogen, phosphorus and potassium were added in hydroponic cultivation through a central composite rotational design. The nitrogen addition rates were 3.30, 5.00, 7.50, 10.00 and 11.70 mmol·L-1; the phosphorus addition rates were 0.66, 1.00, 1.50, 2.00 and 2.34 mmol·L-1, the potassium addition rates were 1.98, 3.00, 4.50, 6.00 and 7.02 mmol·L-1, and fresh grass yield and crude protein content were selected as the optimization criteria. It was found that the quadratic effects of fresh grass yield and crude protein content were extremely significant (P<0.01), with determination coefficients of 0.97; the surface effect of potassium on fresh yield was extremely significant (P<0.01). The interaction effect of nitrogen and potassium on fresh yield was extremely significant (P<0.01), while the interaction between phosphorus and potassium on fresh yield was also significant (P<0.05). The response surface effects of nitrogen-potassium and phosphorus-potassium on crude protein content were extremely significant (P<0.01). The response surface indicated the optimal combination of the three nutrients was nitrogen addition of 9.19 mmol·L-1, phosphorus addition of 1.08 mmol·L-1, and potassium addition of 3.99 mmol·L-1. At this nutrient combination, the modeled fresh yield was maximized at 12.07 kg·plate-1, and the predicted crude protein content was 19.35%DM.

    Trade-off relationships between biomass and nutrient allocation in different natural populations of Agropyron mongolicum on the desert steppe
    Yi-ran CHANG, Jia-mei SHI, Dong-mei XU, Ru-long KANG, Yuan MA
    2024, 33(11):  186-197.  DOI: 10.11686/cyxb2023479
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    In this study, we explored whether Agropyron mongolicum growing on the desert steppe can adapt to environmental changes by adjustments in biomass and nutrient allocation to different plant organs. Across this area, there is a climate transition from semi-arid to arid, and a terrain transition from the Loess plateau to the gently sloping hills of Ordos moving from south to north in Yanchi County, Ningxia. Taking into account the spatio-temporal variations in precipitation and soil across this region, we selected different natural populations of A. mongolicum (populations D, Q, H, and G) sourced from the towns of Dashuikeng, Qingshan, Huamachi, and Gaoshawo, respectively. The allocation to, and trade-off relationships among individual organ biomass and nutrients in roots, stems, leaves, and spike were determined to explore the resource trade-off adaption strategy of A. mongolicum to heterogeneous habitats on the desert steppe. The results showed that: 1) The total individual biomass and root-shoot ratio of A. mongolicum in the H population were 24.69 g·plant-1 and 0.85, respectively, significantly higher than their corresponding values in the D, Q, and G populations (P<0.05). In the D, Q, and G populations, the organs were ranked, from largest to smallest proportion of total biomass of A. mongolicum, as follows: stem>root>spike>leaf. 2) The leaf-spike and leaf-root relationships of A. mongolicum in different natural populations showed significant allometry. In the D and H populations, the organs were ranked from highest rate of biomass accumulation to lowest, as follows: root>leaf>spike>stem. 3) The nitrogen (N) content and N∶phosphorus (P) in each organ were higher, and the carbon (C)∶N in each organ was lower, in the H population than in the other populations. In each natural population of A. mongolicum, the C, N, and P contents were the lowest in roots, N∶P was the highest in leaves, and C∶N and C∶P were the highest in the stem (P<0.05). 4) A. mongolicum adapted to environmental changes mainly through biomass trade-offs among roots, stems, and leaves, and plasticity changes of C content and C∶P in the leaves and N content in the spike. Soil total N and potassium were the main environmental factors affecting the biomass and nutrient allocation patterns in the different natural populations. In summary, A. mongolicum growing on the desert steppe has inherent characteristics of resource trade-offs and allometric growth relationships among the various organs, but at the same time, it can adjust its resource allocation to cope with specialized habitats to a certain extent. This reflects the plasticity and adaptation strategies of different natural populations to a heterogeneous environment.

    Effect of geographic factors on phenotypic traits and seed yield of Elymus nutans on the Tibetan Plateau
    Feng-yu WANG, Guo-ling LIANG, Ze-long HU, Wen-hui LIU
    2024, 33(11):  198-214.  DOI: 10.11686/cyxb2024009
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    This research explored the effects of geographic factors on phenotypic traits and seed yield in Haiyan County, Haibei Tibetan Autonomous Prefecture, Qinghai Province, using 20 lines of Elymus nutans from different sources in the Tibetan Plateau region. A range of geographic locations and altitudinal gradients were selected for the collection of E.nutans, with the aim of providing a theoretical basis for the screening of E.nutans resources and breeding varieties on the Tibetan Plateau. The results were as follows: The coefficients of variation of the phenotypic traits ranged from 6.560% to 33.164%, and the diversity indices of each trait were above 1.4, indicating that the germplasm resources have rich genetic diversity. A structural equation model showed that flag leaf length, inflorescence length, rachis length of the first internode and seed weight per inflorescence were the most important factors affecting the seed yield of E. nutans. Correlation analysis showed that flag leaf length, inflorescence length, rachis length of the first internode, seed weight per inflorescence and seed yield were more affected by geographic factors. Values for all of the above phenotypic traits decreased with increasing altitude and increased with increasing longitude and latitude. Through cluster analysis, it was concluded that plants found in the altitude range of 2600-3350 m, at longitudes near 101° E and in the latitude range between 35°-37° N have excellent values for leaf and spike traits and are rich in varietal type. Therefore this area can be used for the selection and breeding of the germplasm resources of the excellent E. nutans.

    Adaptability evaluation of oat introduction in winter fallow field of Wumeng Mountain area under drought condition
    Xing-fa GAO, Ying-ying NIE, Li-jun XU, Min YANG, Shu-hua XU, Meng ZHU
    2024, 33(11):  215-227.  DOI: 10.11686/cyxb2024119
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    In the Wumeng Mountain area there is an issue of a long fallow period and a short opportunity window for crop production, resulting in a low utilization rate of soil resources in winter fallow fields. In this research, 17 naked oat cultivars and germplasm lines were evaluated for use in winter fallow fields, and the yield, agronomic traits and nutritional quality were determined. Data for the 17 oat varieties were classified and compared by cluster analysis, and a multi-trait evaluation was carried out using grey relational analysis, in order to identify naked oat varieties suitable for planting in winter fallow fields in the Wumeng Mountain area. It was found that: 1) The highest grain yield among the 17 oat varieties was ‘Dingyou No. 13’ (3743 kg·ha-1), followed by ‘Xiangyan No. 18’ (3722 kg·ha-1) and ‘Xiangyan No.13’ (3582 kg·ha-1), which were significantly higher than ‘local oat’ (P<0.05). The variety with the highest hay yield variety was ‘Xiangyan No. 18’ (11172 kg·ha-1), followed by ‘Dingyou No. 7’ and ‘Dingyou No. 5’, which were 10198 and 10029 kg·ha-1, respectively. 2) In terms of nutritional value, ‘Xiangyan No. 18’ had the highest protein and β-glucan content and the lowest starch content, and there was a significant difference between ‘Xiangyan No. 18’ and other oat varieties (P<0.05). The fat content of ‘Dingyou No. 4’ was the highest and significantly higher than that of other oat varieties (P<0.05). 3) The significant positive correlation analysis indicates that grain yield is positively associated with spike length, spikelets per spike, stem thickness, number of tillers, and number of grains, making them the primary factors influencing oat seed production. 4) For the multi-trait grey relational analysis showed that the top 7 oat varieties were: ‘Xiangyan No. 18’‘Dingyou No. 7’‘Dingyou No.13’‘Dingyou No. 11’‘Dingyou No. 5’‘Xiangyan No. 13’ and ‘Bayou No. 14’. Cluster analysis separated the 17 oat varieties into 4 groups. Among them, group I, comprising the top 7 varieties in comprehensive evaluation, accounts for over 70%. The yield of this group was higher, the protein content was generally higher, the number of grains per spike was more, and the content of β-glucan was higher than other groups; therefore this group was defined as a multi-seed, high protein, hypoglycemic group, and included the varieties ‘Xiangyan No.18’‘Dingyou No. 7’‘Dingyou No. 5’‘Xiangyan No. 13’ and ‘Bayou No. 14’. Based on our data, these naked oat varieties can be recommended for planting in winter fallow fields in the Wumeng Mountain area.

    Selection and multi-trait evaluation of silage maize varieties suitable for cultivation in the Qiqihar area
    Zhong-juan ZHANG, Xi-yu HAO, Xue WANG, Feng LI, Wen-long LI
    2024, 33(11):  228-240.  DOI: 10.11686/cyxb2023499
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    With the development of animal husbandry around the city of Qiqihar in Heilongjiang Province, it is urgent to identify silage maize (Zea mays) varieties that are suitable for cultivation in this area to support sustainable development. In this study, 15 maize varieties, including common maize, fresh corn, grain and feed maize, and silage maize were cultivated in a field experiment. Indexes including the growth period, agronomic traits, yield, and nutritional components were measured, and a comparative evaluation using grey relational analysis was conducted to rank the cultivars in terms of their overall production performance. The number of days from sowing to harvest (when the kernel milk line was between 1/2 and 3/4) in the 15 maize varieties ranged from 119 to 134 days, with Jingke silage 932, Jingke 968, and Xianda 304 maturing later than the other 12 varieties. Among all the tested varieties, Jingke silage 932 had the highest values for plant height, ear height, number of green leaves, and ear length. Jingke waxy 768 had the largest stem diameter (3.05 cm). Xianyu 1506 had the shortest ear bald tip length (0.85 cm). The fresh weight yields of Xianda 511, Xianyu 1506, Heyu 321, and Jingke silage 932 were higher than 60 t·ha–1; and the dry matter yields of Lihe 328 (21.42 t·ha–1), Xianyu 1506 (20.59 t·ha–1), Heyu 321 (20.13 t·ha–1), and Xianda 511 (19.84 t·ha–1) were significantly higher than those of the other varieties except Xianda 304 and Jingke silage 932 (P<0.05). The starch content was significantly higher in Xianyu 1506 (34.79%) and Xianda 511 (33.96%) than in the other varieties (P<0.05). Xianda 304 had the lowest contents of neutral detergent fiber and acid detergent fiber; and Xianda 205 had the highest crude protein content (7.44%). According to the results of a comprehensive comparative analysis using grey relational analysis, Xianda 511 and Xianyu 1506 had the highest comprehensive score, demonstrating the best overall production performance. Therefore, these two varieties are recommended as silage maize suitable for cultivation in the third temperature zone of Qiqihar.