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草业学报 ›› 2013, Vol. 22 ›› Issue (5): 104-112.DOI: 10.11686/cyxb20130512

• 研究论文 • 上一篇    下一篇

不同促生菌株组合对紫花苜蓿产量和品质的影响

韩华雯1,2,孙丽娜1,3,姚拓1,2,4*,荣良燕1,5,刘青海1,2,卢虎1,2,马晖玲1,2   

  1. 1.甘肃农业大学草业学院,甘肃 兰州730070;
    2.草业生态系统教育部重点实验室,甘肃 兰州730070;
    3.甘肃省武威市林业局,
    甘肃 武威733000;
    4.中-美草地畜牧业可持续发展研究中心,甘肃 兰州730070;
    5.甘肃省草原推广总站,甘肃 兰州730070
  • 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: E-mail:yaotuo@gsau.edu.cn
  • 作者简介:韩华雯(1989-),女,甘肃庆阳人,在读硕士。
  • 基金资助:
    国家自然基金(30960265),甘肃省农业科技创新项目(GNCX-2012-45),甘肃省农牧厅生物技术专项(GNSW-2011-08)和现代农业产业技术体系(CARS-35)资助。

Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa

HAN Hua-wen1,2, SUN Li-na1,3, YAO Tuo1,2,4, RONG Liang-yan1,5, LIU Qing-hai1,2, LU Hu1,2, MA Hui-ling1,2   

  1. 1.College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China;
    2.Key
    Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou 730070, China;
    3.Forest Bureau of Wuwei City, Wuwei 733000, China;
    4.Sino-U.S.Centers for Sustainable Development of Grassland and Animal Husbandry, Lanzhou 730070, China;
    5.Grassland Promoting Station of Gansu province, Lanzhou 730070, China
  • Online:2013-10-20 Published:2013-10-20

摘要: 利用前期从苜蓿和小麦根际分离的3株溶磷菌(Bacillus sp.,Pseudomonas sp.和Azotobacter sp.)和1株根瘤菌(Sinorhizobium meliloti)的不同组合研制苜蓿根际专用菌肥,并进行田间随机区组试验,测定其对苜蓿产量和品质的影响。结果表明,单一菌株制作的菌肥处理替代半量磷肥处理后,苜蓿的干草产量和品质较CK(全量磷肥)有所下降,但差异不显著;菌株组合制作的菌肥处理替代半量磷肥处理后,苜蓿的干草产量、粗蛋白(CP)、Ca、P和粗脂肪(EE)含量分别较CK提高10.6%,16.4%,14.1%,11.9%和4.2%,酸性洗涤纤维(ADF)和中性洗涤纤维(NDF)含量分别下降10.9%和7.7%。单一菌株的效果不及菌株组合,经分析比较后推荐S7和Jm170+Jm92+Lx191+S7与半量磷肥配施可代替全量磷肥。

Abstract: In recent years, bio-fertilizers have emerged as an important component of the integrated nutrient supply system and hold great promise to improve crop yields because increasing use of chemical fertilizers and high productive systems are creating environmental problems. The effects of different combinations of PGPR strains on yield and quality of alfalfa (Medicago sativa) were studied in randomized block field experiments using bio-fertilizers. These were produced using different combinations of three phosphate solubilizing bacteria (Bacillus sp., Pseudomonas sp. and Azotobacter sp.) and one rhizobium (Sinorhizobium meliloti) strain isolated from the rhizosphere of alfalfa and wheat. Hay yield and quality of alfalfa decreased when treated with mono-strain bio-fertilizer (containing 50% phosphatic fertilizer) compared with the control (100% phosphatic fertilizer) although there was no significant difference. Hay yield, content of crude protein (CP), calcium, phosphate and ether extract (EE) increased 10.6%, 16.4%, 14.1%, 11.9% and 4.2% respectively, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) were reduced by 10.9% and 7.7%. The benefit of mono strain bio-fertilizer was inferior to that of mixed strain bio-fertilizer. Strains S7 and Jm170+Jm92+Lx191+S7 with 50% phosphatic fertilizer are recommended as the best treatments to substitute for 100% phosphatic fertilize in alfalfa production.

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