丛枝菌根真菌对白三叶根系构型和糖含量的影响

doi:10.11686/cyxb20140124

摘要/Abstract

摘要： 以白三叶为材料,在盆栽条件下研究丛枝菌根真菌粘屑多样孢囊霉(Diversispora spurca)、地表球囊霉(Glomus versiforme)和隐类球囊霉(Paraglomus occultum)对其叶绿素含量、根系构型及组织蔗糖和葡萄糖含量的影响。结果表明,接种85 d后白三叶根系菌根侵染率为66.80%~82.51%。3种丛枝菌根真菌都增加地上部、地下部和植株总干物质量,其中地表球囊霉的效果最显著。接种丛枝菌根真菌处理也显著地改善根系构型参数,如长度、投影面积、表面积、体积、根尖数、分支数和交叉数,但因真菌种类而异,其中地表球囊霉的效果最明显。丛枝菌根真菌接种同时显著提高叶绿素含量,达36%~58%,也显著增加叶片和根系的蔗糖和葡萄糖含量,除地表球囊霉侵染的根系蔗糖含量下降外。菌根侵染率与叶片葡萄糖(r=0.852, P<0.01)、叶片蔗糖(r=0.722, P<0.01)和根系葡萄糖(r=0.884, P<0.01)均呈极显著正相关关系。文章讨论了丛枝菌根-糖-根系构型三者的关联。

Abstract: The effects of three arbuscular mycorrhizal fungi (Diversispora spurca,Glomus versiforme and Paraglomus occultum) on chlorophyll content,root system architecture,tissue glucose and sucrose contents of potted white clover (Trifolium repens) were studied. The root mycorrhizal colonization of white clover ranged from 66.80% to 82.51% 85 days after inoculation. The three arbuscular mycorrhizal fungi,especially G. versiforme,significantly increased shoot,root,and total plant dry biomass production. The inoculated treatments also markedly improved traits of root system architecture,including length,projected area,surface area,volume,tips,forkings,and crossings,which are dependent on mycorrhizal fungal species. G. versiforme showed the best effects on root system architecture of the three mycorrhizal fungi. In addition,inoculation with arbuscular mycorrhizal fungi significantly increased leaf chlorophyll content by 36%-58% and sucrose and glucose contents in leaves and roots,except for the sucrose in roots colonized by G. versiforme. Mycorrhizal colonization significantly positively correlated with glucose in leaves (r=0.852,P<0.01),in roots (r=0.884,P<0.01) and sucrose in leaves (r=0.722,P<0.01). The relationships of arbuscular mycorrhizas,sugar,and root system architecture are discussed.

中图分类号:

S816

吴强盛，袁芳英，费永俊，李莉，黄咏明，刘春艳. 丛枝菌根真菌对白三叶根系构型和糖含量的影响[J]. 草业学报, 2014, 23(1): 199-204.

WU Qiang-sheng,YUAN Fang-ying,FEI Yong-jun,LI Li,HUANG Yong-ming,LIU Chun-yan. Effects of arbuscular mycorrhizal fungi on root system architecture and sugar contents of white clover[J]. Acta Prataculturae Sinica, 2014, 23(1): 199-204.

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