甘肃省紫花苜蓿种带促生多粘类芽孢杆菌的分离与鉴定

doi:10.11686/cyxb20140530

摘要/Abstract

摘要：

对来自甘肃省13个紫花苜蓿种植区的39份种子样品进行种带细菌分离,经表型特征和Biolog鉴定,将HTBRC1、HTBRC2、HTBRC3、HTBRC4、HTBRC5、HTBRC6、HTBRC7和HTBRC8初步鉴定为多粘类芽孢杆菌。通过菌种浸种发芽试验发现多粘类芽孢杆菌HTBRC1对紫花苜蓿具有促进生长的作用。试验探明了多粘类芽孢杆菌在甘肃省不同紫花苜蓿种植区所收获种子上的分布;发现分离于紫花苜蓿种子表皮及内部的多粘类芽孢杆菌具有较高耐盐性;为苜蓿生产提供了优质菌种资源及其理论基础。

Abstract:

Thirty nine seed samples from 13 lucerne growing areas of Gansu Province were collected and isolated for seed-borne bacteria. According to phenotypic characteristics and Biolog identification, HTBRC1, HTBRC2, HTBRC3, HTBRC4, HTBRC5, HTBRC6, HTBRC7 and HTBRC8 were preliminarily identified as Paenibacillus polymyxa. A growth promotion function following soaking with strains HTBRC1 was found in a lucerne seed germination experiment. This experiment ascertained the distribution of lucerne seed-borne P. polymyxa in different production regions of Gansu Province and discovered P. polymyxa isolated from the epidermis and internally from lucerne seed with high salt tolerance.

中图分类号:

S816

张振粉,南志标. 甘肃省紫花苜蓿种带促生多粘类芽孢杆菌的分离与鉴定[J]. 草业学报, 2014, 23(5): 256-262.

ZHANG Zhen-fen,NAN Zhi-biao. Isolation and identification of lucerne seed-borne growth promoting Paenibacillus polymyxa in Gansu Province, China[J]. Acta Prataculturae Sinica, 2014, 23(5): 256-262.

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