我国水稻纹枯病菌的融合类群及致病性差异

doi:10.11686/cyxb2017316

草业学报 ›› 2018, Vol. 27 ›› Issue (7): 55-63.DOI: 10.11686/cyxb2017316

我国水稻纹枯病菌的融合类群及致病性差异

王爱军^1,2, 王娜¹, 顾思思³, 赵文娟^1,2, 李平^1,2, 郑爱萍^1,2,*

1.四川农业大学水稻研究所,四川温江 611130;
2.四川农业大学西南作物基因资源与遗传改良教育部重点实验室,四川雅安 625014;
3.四川农业大学风景园林学院,四川温江 611130

收稿日期:2017-07-19 修回日期:2017-09-12 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: *apzh0602@gmail.com
作者简介:王爱军(1988-),男,山西长治人,博士。E-mail: 245491208@qq.com
基金资助:
国家973支撑计划(2014CB160304)和国家自然科学基金(31400130)资助

Anastomosis groups and pathogenicity differentiation of the rice sheath blight pathogen in China

WANG Ai-jun^1,2, WANG Na¹, GU Si-si³, ZHAO Wen-juan^1,2, LI Ping^1,2, ZHENG Ai-ping^1,2,*

1.Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China;
2.Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China;;
3.Landscape Architecture College of Sichuan Agricultural University, Wenjiang 611130, China

Received:2017-07-19 Revised:2017-09-12 Online:2018-07-20 Published:2018-07-20
Contact: *apzh0602@gmail.com

摘要/Abstract

摘要： 为明确我国水稻纹枯病菌的融合类群及其致病力差异,于2015年8月从黑龙江、吉林、辽宁、河南、安徽、浙江、江苏、四川、重庆、贵州、云南、湖北、湖南、广西、广东、福建16个主要水稻种植区采集与水稻纹枯病症状类似的水稻叶鞘和叶片,采用水琼脂分离法分离病菌,在每个产区分离的病菌中各选择1个菌株,采用形态学、菌丝细胞核荧光染色和rDNA-ITS序列分析对分离出的真菌进行鉴定,比较不同地区病原菌生长速度、菌核结构和细胞核数量的差异,并应用柯赫氏法则对不同地区病样中分离的纯培养物致病性进行测定。结果表明,分离自不同地区的61株真菌菌株均为Rhizoctonia solani,且属于AG1IA融合群,菌丝细而松散,菌丝多分枝有隔膜,分枝直角或近45°的锐角,后期产生的菌核大多数细胞为多核,但不同地区的病原菌菌株生长速度、菌核结构和细胞核数量具有一定的差异;在致病性试验中选取的16株菌株均侵染水稻叶片,产生水稻纹枯病典型症状,分离自不同地区的菌株致病力具有明显差异,其中分离自广东、广西、福建和重庆的菌株致病力较强。

关键词: 水稻, 立枯丝核菌, 融合群, 致病性

Abstract: In order to confirm the anastomosis groups of rice sheath blight and their pathogenicity in rice-growing areas of China, samples of rice sheath blight were collected from Heilongjiang, Jilin, Liaoning, Henan, Anhui, Zhejiang, Jiangsu, Sichuan, Chongqing, Guizhou, Yunnan, Hubei, Hunan, Guangxi, Guangdong and Fujian in 2015. The pathogens were isolated from the samples using the water agar separation method. The isolated pathogens were then screened and identified according to morphological characteristics, nucleus fluorescence staining and rDNA-ITS sequence analysis. The growth rate, sclerotium structure and number of cell nuclei of pathogen isolates from different regions were compared. The pathogenicity of these pure cultured strains from the infected rice tissues was tested according to Koch’s law. The sixty-one isolates had loose mycelium and were identified as Rhizoctonia solani AG1IA. Mycelia were much branched and diaphragmatic and were multinucleate. Branches were at right angles or close to 45°. Pathogen isolates from different regions displayed different growth rates, sclerotia structures, and numbers of cell nuclei. Pathogenicity tests showed that 16 of the 61 strains could cause symptoms of rice sheath blight. The pathogenicity of isolates from Guangdong, Guangxi, Fujian and Chongqing was much higher than the pathogenicity of other strains.

Key words: rice, Rhizoctonia solani, anastomosis groups, pathogenicity

王爱军, 王娜, 顾思思, 赵文娟, 李平, 郑爱萍. 我国水稻纹枯病菌的融合类群及致病性差异[J]. 草业学报, 2018, 27(7): 55-63.

WANG Ai-jun, WANG Na, GU Si-si, ZHAO Wen-juan, LI Ping, ZHENG Ai-ping. Anastomosis groups and pathogenicity differentiation of the rice sheath blight pathogen in China[J]. Acta Prataculturae Sinica, 2018, 27(7): 55-63.

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我国水稻纹枯病菌的融合类群及致病性差异

Anastomosis groups and pathogenicity differentiation of the rice sheath blight pathogen in China

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