低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响

doi:10.11686/cyxb2018225

草业学报 ›› 2019, Vol. 28 ›› Issue (4): 81-94.DOI: 10.11686/cyxb2018225

低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响

项洪涛^{1, 2}, 齐德强³, 李琬², 郑殿峰³, 王月溪², 王彤彤², 王立志², 曾宪楠², 杨纯杰², 周行³, 赵海东³

1.黑龙江省农业科学院博士后工作站,黑龙江哈尔滨 150086;
2.黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨 150086;
3.黑龙江八一农垦大学农学院,黑龙江大庆163319

收稿日期:2018-04-11 修回日期:2018-06-25 出版日期:2019-04-20 发布日期:2019-04-20
作者简介:项洪涛(1982-),男,黑龙江哈尔滨人,助理研究员,博士。E-mail: xianght@163.com
基金资助:
中国博士后基金面上项目(2016M591563),黑龙江省博士后资助基金项目(LBH-Z15197),国家自然科学基金项目(31661143012)和黑龙江省农业科学院引进博士人员科研启动金项目(201507-07)资助

Effect of exogenous ABA on the endogenous hormone levels and physiology of chilling resistance in the leaf sheath of rice at the flowering stage under low temperature stress

XIANG Hong-tao^{1, 2}, QI De-qiang³, LI Wan², ZHENG Dian-feng³, WANG Yue-xi², WANG Tong-tong², WANG Li-zhi², ZENG Xian-nan², YANG Chun-jie², ZHOU Hang³, ZHAO Hai-dong³

1.Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, China;
2.Institute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
3.College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received:2018-04-11 Revised:2018-06-25 Online:2019-04-20 Published:2019-04-20

摘要/Abstract

摘要： 为了探讨低温条件下外源ABA对水稻叶鞘内源激素含量和抗寒生理指标的调控效应,以水稻品种龙稻5(耐冷型品种)和龙粳11(冷敏型品种)为材料,于开花期喷施ABA后,在人工气候室进行低温(15 ℃,分别持续1,2,3,4,5 d)处理。结果表明,开花期低温可导致水稻花粉活力和结实率显著降低,冷敏型品种尤为严重,喷施ABA可有效抑制花粉活力和结实率的降低。低温处理可引起水稻叶鞘MDA、可溶性糖、可溶性蛋白和脯氨酸含量及相对电导率的提高,同时导致SOD、POD和CAT活性发生变化;外源ABA具有降低MDA含量和相对电导率的作用,同时促进可溶性糖、可溶性蛋白、脯氨酸含量和保护酶活性的提高。研究证实外源ABA具有提高水稻抗冷的作用,对优化东北地区水稻生产抗冷方法,建立寒地水稻生产的抗冷技术体系,达到保产增产的目的有积极意义。

关键词: 低温, 外源ABA, 水稻, 开花期, 内源激素, 抗寒生理

Abstract: This research investigated the effect of exogenous abscisic acid (ABA) applied to the leaf sheath of rice at low temperature, on endogenous hormone levels, and on a cold hardiness physiology index of two rice varieties. Potted plants of rice varieties, LD5 (cold tolerant) and LJ11 (cold sensitive) were exposed to low temperature (15 ℃; 1, 2, 3, 4, or 5 d) in a climate chamber, at the flowering stage. Low temperature at flowering significantly decreased pollen vigor and seed set, and these effects were particularly serious in the cold sensitive varieties. The exogenous ABA effectively inhibited the decrease of the seed setting rate. Low temperature increased the levels of malondialdehyde (MDA), soluble sugar, soluble protein, and proline, and the relative conductivity of the leaf sheath, and also led to changes in the activities of superoxide dismutase, peroxidase and catalase. Exogenous ABA reduced the level of MDA and the relative conductivity, and promoted an increase in soluble sugar, soluble protein and proline levels, and promoted improvements in protective enzyme activity. This study confirmed that exogenous ABA improves the cold resistance of rice. Enhancement of low temperature resistance in rice will be very significant in facilitating increased rice production in the colder northeast east regions of China.

Key words: low temperature, exogenous ABA, rice, flowering stage, endogenous hormone, chilling resistance

项洪涛, 齐德强, 李琬, 郑殿峰, 王月溪, 王彤彤, 王立志, 曾宪楠, 杨纯杰, 周行, 赵海东. 低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响[J]. 草业学报, 2019, 28(4): 81-94.

XIANG Hong-tao, QI De-qiang, LI Wan, ZHENG Dian-feng, WANG Yue-xi, WANG Tong-tong, WANG Li-zhi, ZENG Xian-nan, YANG Chun-jie, ZHOU Hang, ZHAO Hai-dong. Effect of exogenous ABA on the endogenous hormone levels and physiology of chilling resistance in the leaf sheath of rice at the flowering stage under low temperature stress[J]. Acta Prataculturae Sinica, 2019, 28(4): 81-94.

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低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响

Effect of exogenous ABA on the endogenous hormone levels and physiology of chilling resistance in the leaf sheath of rice at the flowering stage under low temperature stress

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[1]	舒新月, 江波, 马丽, 郑爱萍. 不同侵染时间点稻粒黑粉病菌的转录组分析[J]. 草业学报, 2020, 29(9): 190-202.
[2]	王玉萍, 郜春晓, 王盛祥, 何晓童. 低温弱光胁迫下芸豆叶片光抑制与类囊体膜脂构成变化[J]. 草业学报, 2020, 29(8): 116-125.
[3]	刘燕, 于美玲, 张然, 牛奎举, 李玉珠, 张金青, 马晖玲. 甘肃野生草地早熟禾内源激素含量的变化与无融合生殖率的关系研究[J]. 草业学报, 2020, 29(7): 99-111.
[4]	鲍根生, 宋梅玲, 王玉琴, 刘静, 王宏生. 不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响[J]. 草业学报, 2020, 29(4): 147-156.
[5]	衣琨, 赵一航, 胡尧, 刘佳雪, 贺涛涛, 李旭, 宋鹏, 崔国文, 殷秀杰. GA₃和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响[J]. 草业学报, 2020, 29(2): 22-30.
[6]	王宁, 付亚军, 袁美丽, 刘征阳, 张铭鑫, 米银法. GA₃浸种对入侵植物节节麦种子破眠及发芽特性的影响[J]. 草业学报, 2020, 29(2): 73-81.
[7]	张翔, 杨勇, 刘学勇, 向佐湘. 外源水杨酸对低温胁迫下海滨雀稗抗寒生理特征的影响[J]. 草业学报, 2020, 29(1): 117-124.
[8]	项洪涛, 李琬, 何宁, 王雪扬, 郑殿峰, 王彤彤, 梁晓艳, 唐晓东, 李一丹. 苗期低温胁迫下烯效唑对红小豆根系抗寒生理及产量的影响[J]. 草业学报, 2019, 28(7): 92-102.
[9]	王沛, 陈玖红, 王平, 马清, 田莉华, 陈有军, 周青平. 披碱草属植物抗逆性研究现状和存在的问题[J]. 草业学报, 2019, 28(5): 151-162.
[10]	罗颖洁, 陈桂华, 穆麟, 胡龙兴, 张志飞, 高帅, 魏仲珊. 不同稻秸添加比例对紫花苜蓿和麦麸混合青贮的影响[J]. 草业学报, 2019, 28(5): 178-184.
[11]	孙亚男, 林茹, 潘晓阳, 陈月, 陶磊, 郭长虹. 紫花苜蓿MsZAT10基因的克隆及其在烟草中的功能验证[J]. 草业学报, 2019, 28(12): 94-102.
[12]	石正海, 刘文辉, 张永超, 秦燕, 魏小星. 氮磷肥配施对西北羊茅开花期叶片光合特性日变化的影响[J]. 草业学报, 2019, 28(11): 75-85.
[13]	万琪慧, 马黎华, 蒋先军. 垄作免耕对水稻根系特性和氮磷钾养分累积的影响[J]. 草业学报, 2019, 28(10): 44-52.
[14]	王爱军, 王娜, 顾思思, 赵文娟, 李平, 郑爱萍. 我国水稻纹枯病菌的融合类群及致病性差异[J]. 草业学报, 2018, 27(7): 55-63.
[15]	朱菊华, 孙星, 许斌, 梁婷, 刘明, 缪建, 赵耕毛. 不同基因型菊芋耐盐生理及其生态适应性研究[J]. 草业学报, 2018, 27(6): 120-127.