GA3和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响

doi:10.11686/cyxb2019317

摘要/Abstract

摘要： 为了研究植物生长调节剂赤霉素(GA₃)和6-苄氨基腺嘌呤(6-BA)对高加索三叶草根蘖芽生长发育及内源激素含量的影响,在不同浓度的GA₃(0对照、300、600、900 和1200 mg·L^-1)和6-BA(0对照、25、50、100和200 mg·L^-1),对其根蘖芽生长后的植株高度、叶片数、叶绿素含量和内源激素含量(地上部分和地下部分)进行测定与分析。结果表明:与对照相比,所有GA₃处理均显著增加了株高(P<0.05)。尤其在600 mg·L^-1 GA₃处理下,平均株高为21.33 cm(高于对照处理4.12倍)。GA₃还促进了叶绿素a的合成,最显著效果是在600 mg·L^-1 GA₃处理下,叶绿素a的浓度为0.92 mg·g^-1(高于对照76.92%) (P<0.05)。GA₃对叶绿素b含量无显著影响(P>0.05)。在6-BA所有处理浓度下,株高约为对照处理2.6倍,并且对于叶片数,6-BA处理比GA₃处理更敏感,在200 mg·L^-1时最多,高于对照2.99倍。 6-BA显著促进了叶绿素a和b的积累(P<0.05)。内源激素含量在整个处理浓度下均显示出对外源激素应用的剂量反应。对于地上植物部分,600 mg·L^-1的外源GA₃引起内源玉米素(ZT),GA₃和生长素(IAA)分别增加约23.84%,55.71%和137.99%,而脱落酸(ABA)减少约44.09%。与对照相比,外源200 mg·L^-1 6-BA使内源ZT,GA₃和IAA含量分别提高了约293.23%,49.71%和49.84%,而ABA降低了约52.48%。对于地下植物部分,内源激素变化与地上部分植物的响应大致相似,明显不同在于外源同样浓度GA₃(600 mg·L^-1)和6-BA(200 mg·L^-1)处理引起的ABA降低要少(分别为15.15%和28.26%)。总之,600 mg·L^-1 GA₃和200 mg·L^-1 6-BA是促高加索生长发育的最佳浓度。

关键词: 高加索三叶草, 根蘖芽, 赤霉素(GA₃), 6-苄氨基腺嘌呤(6-BA), 内源激素

Abstract: This study explored the effects of gibberellin (GA₃) and 6-benzylaminoadenine (6-BA) plant growth regulator on the growth and endogenous hormone content of rhizome of Caucasian clover. Plant height, leaf number, chlorophyll and endogenous hormone contents (in above and below ground parts of Caucasian clover) were characterized under different concentrations of GA₃ (0 control, 300, 600, 900, and 1200 mg·L^-1) and 6-BA (0 control, 25, 50, 100 and 200 mg·L^-1). All GA₃ treatments dramatically increased plant height compared to the control (P<0.05). Under the 600 mg·L^-1 GA₃ treatment, mean plant height was 21.33 cm (4.12 times higher than the control treatment). Similar responses were seen at the other GA₃ concentrations(300, 900 and 1200 mg·L^-1). GA₃ also promoted the synthesis of chlorophyll a; the most significant treatment effect was a concentration of 0.92 mg·g^-1 (76.92% higher than the control treatment) found in the 600 mg·L^-1 GA₃ treatment (P<0.05). GA₃ had no significant effect on chlorophyll b content (P>0.05). Under 6-BA treatment, plant height was approximately 2.6 times that of the control treatment at all concentrations tested, and leaf number was more responsive than in the GA₃ treatments, being the highest (2.99 times higher than the control) at 200 mg·L^-1 concentration. 6-BA significantly promoted the accumulation of chlorophyll a and b (P<0.05). In general, endogenous hormone levels showed a dose response to exogenous hormone application across the concentrations tested. For the aboveground plant parts, exogenous GA₃ at 600 mg·L^-1 elicited increases in zeatin (ZT), GA₃ and auxin (IAA) of approximately 23.84%, 55.71% and 137.99%, respectively, and a decrease in abscisic acid (ABA) of approximately 44.09%; while exogenous 6-BA at 200 mg·L^-1 increased endogenous ZT, GA₃ and IAA levels by approximately 293.23%, 49.71% and 49.83%, respectively, and decreased ABA by approximately 52.48%, compared to control treatments. For the below ground plant parts, endogenous hormone responses were broadly similar to those in above ground parts, except that the ABA decrease from exogenous GA₃ (600 mg·L^-1) and 6-BA (200 mg·L^-1) application was much less (15.15% and 28.26%, respectively). In summary, 600 mg·L^-1 GA₃ and 200 mg·L^-1 6-BA are optimal concentrations for promotion of the growth and development of Caucasian clover.

Key words: Caucasian clover (Trifolium ambiguum), rhizome segment, GA₃, 6-BA, endogenous hormone

衣琨, 赵一航, 胡尧, 刘佳雪, 贺涛涛, 李旭, 宋鹏, 崔国文, 殷秀杰. GA₃和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响[J]. 草业学报, 2020, 29(2): 22-30.

YI Kun, ZHAO Yi-hang, HU Yao, LIU Jia-xue, HE Tao-tao, LI Xu, SONG Peng, CUI Guo-wen, YIN Xiu-jie. Effect of GA₃ and 6-BA on rhizome segment growth and endogenous hormone content of Caucasian clover[J]. Acta Prataculturae Sinica, 2020, 29(2): 22-30.

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GA₃和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响

Effect of GA₃ and 6-BA on rhizome segment growth and endogenous hormone content of Caucasian clover

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