30个紫花苜蓿品种对苜蓿花叶病毒病的田间抗性初步研究

doi:10.11686/cyxb2018259

草业学报 ›› 2019, Vol. 28 ›› Issue (4): 157-167.DOI: 10.11686/cyxb2018259

30个紫花苜蓿品种对苜蓿花叶病毒病的田间抗性初步研究

郭志鹏¹, 冯长松², 张靖雪¹, 王苗利¹, 曲根¹, 刘建宇¹, 管永卓¹, 张晓婷¹, 郭玉霞^{1, *}, 严学兵^{3, *}

1.河南农业大学牧医工程学院,河南郑州 450002;
2.河南省农业科学院畜牧所,河南郑州 450002;
3.扬州大学动物科学与技术学院,江苏扬州 225000

收稿日期:2018-04-24 修回日期:2018-09-25 出版日期:2019-04-20 发布日期:2019-04-20
通讯作者: E-mail: yuxiaguo@163.com; yxbbjzz@163.com
作者简介:郭志鹏(1991-),男,河南焦作人,在读硕士。Email: 15839186156@163.com
基金资助:
国家自然科学基金项目(31502006),国家重点研发计划项目(2017YFD0502104)和河南省高等学校重点科研项目(15A230019)资助

Field resistance to alfalfa mosaic virus among 30 alfalfa varieties

GUO Zhi-peng¹, FENG Chang-song², ZHANG Jing-xue¹, WANG Miao-li¹, QU Gen¹, LIU Jian-yu¹, GUAN Yong-zhuo¹, ZHANG Xiao-ting¹, GUO Yu-xia^{1, *}, YAN Xue-bing^{3, *}

1.College of Animal and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China;
2.Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
3.College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, China

Received:2018-04-24 Revised:2018-09-25 Online:2019-04-20 Published:2019-04-20

摘要/Abstract

摘要： 为了比较不同紫花苜蓿品种对病毒病的抗性,对30个紫花苜蓿品种的病毒病发病情况进行了田间调查,利用小RNA深度测序技术结合RT-PCR技术检测鉴定各紫花苜蓿品种中的病毒种类,并测定了各紫花苜蓿品种的发病率、病情指数、相对株高、相对单株干重、相对茎秆直径、相对叶面积、相对叶长和相对叶宽,综合比较各紫花苜蓿品种的抗性,并对部分指标进行系统聚类分析。结果表明:30个紫花苜蓿品种均只受到苜蓿花叶病毒(alfalfa mosaic virus, AMV)的侵染,所有紫花苜蓿品种的田间发病率均超过94%,病情指数范围为31.53~55.50;30个紫花苜蓿品种中,抗性较强的品种有1个,为射手2号,抗性一般的品种有6个,其余23个品种的抗性较差,分别占供试品种的3.33%,20.00%和76.67%;聚类分析结果表明,抗性较强的射手2号与其他紫花苜蓿品种的亲缘关系较远,与其杂交有可能产生较大的杂种优势。

关键词: 苜蓿品种, 病毒病, 抗性, 聚类分析

Abstract: The objective of this study was to compare the resistance of different alfalfa varieties to virus disease. Virus loads of 30 alfalfa varieties were investigated in plants in the field. The virus species present in the alfalfa plants were detected and identified using small RNA high-throughput sequencing and RT-PCR technology. Data collected for the 30 alfalfa varieties included the virus incidence rate, disease index, relative plant height, relative dry weight per plant, relative stem diameter, relative leaf area, relative leaf length and relative leaf width, and these data were used to comprehensively compare the disease resistance of the 30 tested varieties. Cluster analysis of some traits was also carried out. Among the 30 tested alfalfa varieties, only alfalfa mosaic virus (AMV) infection was detected. The field incidence of AMV in all alfalfa varieties was more than 94%, and the values of the multivariate disease index ranged from 31.53 to 55.50. There was 1 resistant variety (Sheshou No.2); there were 6 partially resistant varieties, and 23 susceptible varieties. The cluster analysis indicated that Sheshou No.2 had an AMV resistance profile far different from all other alfalfa varieties, as indicated by the measured traits. It can be speculated that hybridization of other varieties with Sheshou No. 2 would produce high heterosis.

Key words: alfalfa variety, virus disease, resistance, cluster analysis

郭志鹏, 冯长松, 张靖雪, 王苗利, 曲根, 刘建宇, 管永卓, 张晓婷, 郭玉霞, 严学兵. 30个紫花苜蓿品种对苜蓿花叶病毒病的田间抗性初步研究[J]. 草业学报, 2019, 28(4): 157-167.

GUO Zhi-peng, FENG Chang-song, ZHANG Jing-xue, WANG Miao-li, QU Gen, LIU Jian-yu, GUAN Yong-zhuo, ZHANG Xiao-ting, GUO Yu-xia, YAN Xue-bing. Field resistance to alfalfa mosaic virus among 30 alfalfa varieties[J]. Acta Prataculturae Sinica, 2019, 28(4): 157-167.

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30个紫花苜蓿品种对苜蓿花叶病毒病的田间抗性初步研究

Field resistance to alfalfa mosaic virus among 30 alfalfa varieties

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