外源复合激素对匍匐翦股颖抗褐斑病的诱导抗性

doi:10.11686/cyxb2018255

草业学报 ›› 2018, Vol. 27 ›› Issue (11): 120-130.DOI: 10.11686/cyxb2018255

外源复合激素对匍匐翦股颖抗褐斑病的诱导抗性

赵春旭^{1*, *}, 姜寒玉^{2*, *}, 董文科¹, 陈红², 方彦霞², 谢丽萍², 马晖玲^{1, *}

1.甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州 730070;
2.甘肃农业大学生命科学技术学院,甘肃兰州 730070

收稿日期:2018-04-24 修回日期:2018-06-12 出版日期:2018-11-20 发布日期:2018-11-20
通讯作者: mahl@gsau.edu.cn
作者简介:赵春旭(1985-),男,甘肃兰州人,在读博士。E-mail: zhaocx@gsau.edu.cn;姜寒玉(1980-),女,甘肃定西人,副教授,博士。E-mail:jianghy@gsau.edu.cn。**共同第一作者These authors contributed equally to this work.
基金资助:
国家自然科学基金(31502012)资助

Effects of composite exogenous hormone application on induction of systemic resistance to Rhizoctonia solani in creeping bentgrass

ZHAO Chun-xu^{1, **}, JIANG Han-yu^{2, **}, DONG Wen-ke¹, CHEN Hong², FANG Yan-xia², XIE Li-ping², MA Hui-ling^{1, *}

1.College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
2.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2018-04-24 Revised:2018-06-12 Online:2018-11-20 Published:2018-11-20

摘要/Abstract

摘要： 以匍匐翦股颖品种“Penn-A4”为材料,研究以不同浓度的油菜素内酯(EBR)、水杨酸(SA)和乙烯(ET)组合的外源复合激素和3个连续诱导时间(5、7、10 d)为变量,通过测定病情指数、防治效果、抗氧化酶活性及抗坏血酸-谷胱甘肽循环变化,分析外源复合激素及连续诱导时间对匍匐翦股颖抗褐斑病的诱导效应。结果表明,不同激素组合和不同连续诱导时间处理均可提高匍匐翦股颖的抗病性,其中ET+EBR组合连续诱导处理7 d的病情指数最低,为13.36,显著低于其他处理,此时防治效果为40.51%;另外,此组合诱导处理后,匍匐翦股颖幼苗叶片的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)分别比对照(CK)提高了130.53%、197.40%和101.66%;抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性分别比对照(CK)提高了316.26%、41.95%和80.34%;脱氧抗坏血酸(AsA)、氧化型谷胱甘肽(GSH)含量以及脱氧抗坏血酸/氧化型抗坏血酸(AsA/DHA)、氧化型谷胱甘肽/还原型谷胱甘肽(GSH/GSSG)也均高于对照(CK)和其他处理。表明EBR、SA和ET三种物质组成的复合外源物可以诱导匍匐翦股颖抗褐斑病,其中ET+EBR组合连续诱导处理7 d的效果最好。该研究结果为新型复合诱导抗病剂的应用推广提供了技术支持。

关键词: 匍匐翦股颖, 外源复合激素, 褐斑病, 诱导抗性, 抗氧化系统, 抗坏血酸-谷胱甘肽循环

Abstract: Composite plant hormone formulations with different concentrations of epibrassinolide (EBR), salicylic acid (SA) and ethephon (ET) were applied exogenously to “Penn-A4” bentgrass with induction times of 5, 7, and 10 days to test if the hormone treatments could enhance disease resistance. The effect of the exogenous composite hormone application and induction time treatments on the brown spot resistance of creeping bentgrass was assessed by determining a disease index, the control efficacy, antioxidant enzyme activity and ascorbic acid/glutathione cycle changes. Some treatments did improve the disease resistance of creeping bentgrass. The disease index for an ET+EBR hormone treatment with 7 days induction was significantly lower than other treatments, and the anti-disease effect was quantified at 40.51%. Moreover, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities increased by 130.53%, 197.40% and 101.66%, respectively, while ascorbate peroxidase (APX), dehydroascorbate reduction (DHAR), and glutathione reductase (GR) activities increased by 316.26%, 41.95% and 80.34%, respectively, compared with the control, after this treatment. In addition, active deoxyascorbic acid (AsA), oxidized glutathione (GSH) content, and AsA/hydroascorbic acid (DHA), and GSH/reduced glutathione (GSSG) ratios were all higher for the ET+EBR combination, than for the control and other treatments. In summary, exogenous application of mixtures of EBR, SA and ET were able to induce brown spot resistance in creeping bentgrass, and an ET+EBR combination with an induction period of 7 days had the best effect. The results of this study provide technical information for the development and promotion of novel hormone-based agents for induction of plant disease resistance.

Key words: creeping bentgrass, composite exogenous hormone, Rhizoctonia solani, induced resistance, antioxidant enzymes, ascorbate-glutathione cycle

赵春旭, 姜寒玉, 董文科, 陈红, 方彦霞, 谢丽萍, 马晖玲. 外源复合激素对匍匐翦股颖抗褐斑病的诱导抗性[J]. 草业学报, 2018, 27(11): 120-130.

ZHAO Chun-xu, JIANG Han-yu, DONG Wen-ke, CHEN Hong, FANG Yan-xia, XIE Li-ping, MA Hui-ling. Effects of composite exogenous hormone application on induction of systemic resistance to Rhizoctonia solani in creeping bentgrass[J]. Acta Prataculturae Sinica, 2018, 27(11): 120-130.

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外源复合激素对匍匐翦股颖抗褐斑病的诱导抗性

Effects of composite exogenous hormone application on induction of systemic resistance to Rhizoctonia solani in creeping bentgrass

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