箭筈豌豆新品种DUS测试指南研制—测试性状评价和参照品种筛选

doi:10.11686/cyxb2019303

草业学报 ›› 2019, Vol. 28 ›› Issue (11): 133-146.DOI: 10.11686/cyxb2019303

箭筈豌豆新品种DUS测试指南研制—测试性状评价和参照品种筛选

闵学阳¹, 刘文献¹, 王彦荣^1,*, 林晓珊¹, 齐晓², 张正社¹, 聂斌¹

1.兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020;
2.全国畜牧总站,全国草品种审定委员会办公室,北京 100125

收稿日期:2019-07-01 出版日期:2019-11-20 发布日期:2019-11-20
通讯作者: *. E-mail: yrwang@lzu.edu.cn
作者简介:闵学阳(1991-),男,甘肃张掖人,在读博士。E-mail: minxy15@lzu.edu.cn
基金资助:
国家草品种区域试验项目(农财发[2018]33号)和甘肃省农业科技创新项目(GNCX-2016-10)资助

Test guidelines for distinctness, uniformity, and stability of new varieties of Vicia sativa-Testing characteristics and assessment of reference varieties

MIN Xue-yang¹, LIU Wen-xian¹, WANG Yan-rong^1,*, LIN Xiao-shan¹, QI Xiao², ZHANG Zheng-she¹, NIE Bin¹

1.State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.National Animal Husbandry Service, Office of the Chinese Herbage Cultivar Registration Board, Beijing 100125, China

Received:2019-07-01 Online:2019-11-20 Published:2019-11-20
Contact: *. E-mail: yrwang@lzu.edu.cn

摘要/Abstract

摘要： 以国际植物新品种保护联盟(UPOV)制定的植物新品种DUS(distinctness, uniformity and stability)测试指南总则(TG/1/3)、箭筈豌豆DUS测试指南(TG/32/7)等技术文件为指导,对箭筈豌豆DUS测试指南的核心技术内容:测试性状和参照品种等开展了研究。在国内外共收集到51个箭筈豌豆品种,并进行连续2年的田间观测。观测性状总计31个,包括UPOV箭筈豌豆DUS测试指南中的23个性状,以及本实验发现有特异性的8个新增性状。对每个测试性状的表达状态、观测时期、测定方法、观测数量等进行了分级和详尽描述。共筛选出32个参照品种(国际21个, 我国11个),确定了123个表达状态。并对UPOV 箭筈豌豆DUS测试指南中“外种皮底色”和“种子形状”表达状态的分级进行了补充。本研究结果可为我国箭筈豌豆新品种 DUS 测试指南的制定奠定技术基础,对促进我国箭筈豌豆品种保护、审定和育种工作具有重要的意义。

关键词: 箭筈豌豆, DUS测试, UPOV, 性状, 参照品种

Abstract: We used the general introduction to the examination of distinctness, uniformity, and stability (TG/1/3), guidelines to identify and conduct tests for distinctness, uniformity, and stability of common vetch (TG/32/7), and related documents developed by the International Union for the Protection of New Varieties of Plants (UPOV) as guidelines to study the core technical content of the common vetch DUS test guidelines, which include test characteristics and reference varieties. We collected 51 common vetch varieties from around the world and evaluated their characteristics in a 2-year field trial. We evaluated 31 test characteristics, including 23 UPOV common vetch DUS test characteristics, and eight new characteristics representative of the distinctness feature. The expression status, and the observation period/method/number of each characteristic were scientifically graded and accurately described. Finally, a total of 32 reference varieties (21 international varieties and 11 varieties from China) corresponding to 123 expression states were screened. We further complemented the expression states of “ground color of test” and “seed shape” in the UPOV common vetch DUS test guidelines. This study lays a technical foundation for the development of new common vetch varieties in accordance with DUS test guidelines in China. The establishment of these reference varieties and testing guidelines will be useful for the protection, validation, and breeding of common vetch in China.

Key words: Vicia sativa, DUS test, UPOV, characteristics, reference variety

闵学阳, 刘文献, 王彦荣, 林晓珊, 齐晓, 张正社, 聂斌. 箭筈豌豆新品种DUS测试指南研制—测试性状评价和参照品种筛选[J]. 草业学报, 2019, 28(11): 133-146.

MIN Xue-yang, LIU Wen-xian, WANG Yan-rong, LIN Xiao-shan, QI Xiao, ZHANG Zheng-she, NIE Bin. Test guidelines for distinctness, uniformity, and stability of new varieties of Vicia sativa-Testing characteristics and assessment of reference varieties[J]. Acta Prataculturae Sinica, 2019, 28(11): 133-146.

参考文献

[1] Zhu Y, Zhou X C, Song M.Research on progress and influence of plant variety protection on agriculture of China. Management of Agricultural Science and Technology, 2017, 36(6): 1-7.
朱岩, 周绪晨, 宋敏. 中国农业植物新品种保护进展及影响研究. 农业科技管理, 2017, 36(6): 1-7.
[2] Zhong H F, Huang M L, Zhong H Q, et al. The development status of agricultural varieties protection and DUS testing techniques in China. Chinese Journal of Tropical Crops, 2017, 38(6): 1155-1162.
钟海丰, 黄敏玲, 钟淮钦, 等. 中国农业植物新品种保护与DUS测试技术发展现状.热带作物学报, 2017, 38(6): 1155-1162.
[3] Wang Y R, Cui Y H, Nan Z B, et al. The traits select and standard samples determine in the DUS test guideline for new plant varieties. Pratacultural Science, 2002, 19(2): 44-47.
王彦荣, 崔野韩, 南志标, 等. 植物新品种DUS测试指南中的性状选择与标样品种确定.草业科学, 2002, 19(2): 44-47.
[4] Ministry of Agriculture and Rural Affairs of the People’s Republic of China. The seed law of the People’s Republic of China[EB/OL]. (2015-11-05). http://www.moa.gov.cn/sjzz/zzj/zcfgzzj/zmfl/201703/t20170310_5517107.htm.
中华人民共和国农业部. 中华人民共和国种子法[EB/OL]. (2015-11-05). http://www.moa.gov.cn/sjzz/zzj/zcfgzzj/zmfl/201703/t20170310_5517107.htm.
[5] Wang Y R, Nan Z B, Sun J H, et al. Guidelines for the conduct of tests for distinctness, uniformity, and stability of new varieties of Poa pratensis in China. Pratacultural Science, 2003, 20(12): 58-66.
王彦荣, 南志标, 孙建华, 等. 草地早熟禾新品种特异性、一致性和稳定性测试指南初报. 草业科学, 2003, 20(12): 58-66.
[6] Wang Y R, Ren J Z, Sun J H.et al. Guidelines for the conduct of tests for distinctness, uniformity and stability of new varieties of Medicago sativa and M.varia in China. Pratacultural Science, 2002, 19(12): 16-23.
王彦荣, 任继周, 孙建华, 等. 苜蓿新品种特异性、一致性和稳定性测试指南初报. 草业科学, 2002, 19(12): 16-23.
[7] Nan Z B, Zhang J Y, Wang Y R, et al. Genotype×environment interactions and consistency analysis for agronomic characteristics of five Vicia sativa lines. Acta Ecologica Sinica, 2004, 24(3): 395-401.
南志标, 张吉宇, 王彦荣, 等. 五个箭筈豌豆品系基因型与环境互作效应及农艺性状稳定性.生态学报, 2004, 24(3): 395-401.
[8] Dong R, Jahufer M, Dong D K, et al. Characterisation of the morphological variation for seed traits among 537 germplasm accessions of common vetch (Vicia sativa L.) using digital image analysis. New Zealand Journal of Agricultural Research, 2016, 59(4): 422-435.
[9] Min X Y, Wei X Y, Liu W X, et al. Analysis of genetic differences and construction of an SSR marker fingerprint for Vicia sativa varieties. Acta Prataculturae Sinica, 2019, 28(4): 116-128.
闵学阳, 韦兴燚, 刘文献, 等. 箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建. 草业学报, 2019, 28(4): 116-128.
[10] Wang Y R, Nan Z B, Nie B.et al. Distinct comparison of morphological characteristics for several new cold resistant Vicia sativa lines. Acta Prataculturae Sinica, 2005, 14(2): 28-32.
王彦荣, 南志标, 聂斌, 等. 几种抗寒春箭舌豌豆新品系的形态特异性比较. 草业学报, 2005, 14(2): 28-32.
[11] Li L L, Hua D F, Zheng X W, et al. Effects of moisture content and sowing-mix ratio on the quality of baled oat and common vetch/hairy vetch silage mixtures in the pastoral area of southern Qinghai. Acta Prataculturae Sinica, 2018, 27(7): 166-174.
李蕾蕾, 花登峰, 郑兴卫, 等. 含水量和混播比例对青南牧区燕麦-箭筈豌豆/毛苕子混播青贮品质的影响. 草业学报, 2018, 27(7): 166-174.
[12] International Union for the Protection of New Varieties of Plants(UPOV). Guidelines for the conduct of tests for distinctness, uniformity and stability: Common vetch, TG/32/7. Geneva: International Union for the Protection of New Varieties of Plants, 2013.
[13] Wang Y R, Yu L, Sun J H, et al. Guidelines for the conduct of tests for distinctness, uniformity and stability lucerne (Medicago sativa L. & Medicago×varia Martyn) , NY/T 2747-2015. Beijing: Ministry of Agriculture of the People’s Republic of China, 2015.
王彦荣, 余玲, 孙建华, 等. 植物新品种特异性、一致性和稳定性测试指南(紫花苜蓿和杂花苜蓿), NY/T 2747-2015. 北京: 中华人民共和国农业部, 2015.
[14] Wang Y R, Yu L, Liu W X, et al. Guidelines for the conduct of tests for distinctness, uniformity and stability-Kentucky bluegrass, NY/T 2428-2013. Beijing: Ministry of Agriculture of the People’s Republic of China, 2013.
王彦荣, 余玲, 刘文献, 等. 植物新品种特异性、一致性和稳定性测试指南(草地早熟禾), NY/T 2428-2013. 北京: 中华人民共和国农业部, 2013.
[15] International Union for the Protection of New Varieties of Plants(UPOV). Development of test guidelines, TGP/7/1. Geneva: International Union for the Protection of New Varieties of Plants, 2004.
[16] International Union for the Protection of New Varieties of Plants(UPOV). Examining distinctness, TGP/9. Geneva: International Union for the Protection of New Varieties of Plants, 2007.
[17] International Union for the Protection of New Varieties of Plants(UPOV). Examining uniformity, TGP/10. Geneva: International Union for the Protection of New Varieties of Plants, 2007.
[18] International Union for the Protection of New Varieties of Plants (UPOV). Examining Stability, TGP/11. Geneva: International Union for the Protection of New Varieties of Plants, 2007.
[19] Dong R.Genetic diversity and pod shattering biological characteristics study of common vetch (Vicia sativa L.). Lanzhou: Lanzhou University, 2017.
董瑞. 箭筈豌豆遗传多样性及裂荚生物学特性研究. 兰州: 兰州大学, 2017.
[20] Tang H.General introduction to the distinctness, uniformity and stability of new varieties of plants. Beijing: China Agricultural Press, 2017: 18-19.
唐浩. 植物品种特异性、一致性、稳定性测试总论. 北京: 中国农业出版社, 2017: 18-19.
[21] Xu L, Luo X Y, Liu D F, et al. Guidelines for the conduct of tests for distinctness, uniformity and stability stylo [Stylosanthes guianensis (Aubl) Sw., S. hamata (L.) Taub., S. scabra Vog., S. capitate Vog.], NY/T 3434-2019. Beijing: Ministry of Agriculture of the People’s Republic of China, 2019.
徐丽, 罗小燕, 刘迪发, 等. 植物新品种特异性、一致性和稳定性测试指南(柱花草属), NY/T 3434-2019. 北京: 中华人民共和国农业部, 2019.
[22] Wang J L, Sun L F, Shen Z B, et al. Guidelines for the conduct of tests for distinctness, uniformity and stability-white clover (Trifolium repens L.), NY/T 2565-2014. Beijing: Ministry of Agriculture of the People’s Republic of China, 2014.
王建丽, 孙连发, 申忠宝, 等. 植物新品种特异性、一致性和稳定性测试指南(白三叶), NY/T 2565-2014. 北京: 中华人民共和国农业部, 2014.
[23] Shen Z B, Liu P, Wang J L, et al. Guidelines for the conduct of tests for distinctness, uniformity and stability-red clover (Trifolium pretense L.), NY/T 2480-2013. Beijing: Ministry of Agriculture of the People’s Republic of China, 2013.
申忠宝, 刘平, 王建丽, 等. 植物新品种特异性、一致性和稳定性测试指南(红三叶), NY/T 2480-2013. 北京: 中华人民共和国农业部, 2013.
[24] Sm$\acute{y}$kal P, Coyne C J, Ambrose M J, et al. Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences, 2015, 34(1/2/3): 43-104.

箭筈豌豆新品种DUS测试指南研制—测试性状评价和参照品种筛选

Test guidelines for distinctness, uniformity, and stability of new varieties of Vicia sativa-Testing characteristics and assessment of reference varieties

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	吴晓娟, 杨梅, 芦奕晓, 杨惠敏. 混播比例和施氮肥对箭筈豌豆/燕麦草地根系特性的影响[J]. 草业学报, 2020, 29(9): 106-116.
[2]	张雨桐, 石凤翎. 株型形成及牧草株型相关研究进展[J]. 草业学报, 2020, 29(9): 203-214.
[3]	王琇瑜, 黄晓霞, 和克俭, 孙晓能, 吕曾哲舟, 张勇, 朱湄, 曾睿钦. 滇西北高寒草甸植物群落功能性状与土壤理化性质的关系[J]. 草业学报, 2020, 29(8): 6-17.
[4]	再吐尼古丽·库尔班, 吐尔逊·吐尔洪, 涂振东, 王卉, 山其米克, 艾克拜尔·伊拉洪. 长期不同施肥处理对连作高粱生长规律及产量的影响研究[J]. 草业学报, 2020, 29(8): 81-92.
[5]	王延翠, 于伟丽, 王树楷, 葛春霞, 张国斌, 陈翠霞. 芒属植物分蘖数性状的QTL定位[J]. 草业学报, 2020, 29(7): 52-59.
[6]	王琼, 段廷玉, 南志标. 箭筈豌豆炭疽病病原菌分离鉴定[J]. 草业学报, 2020, 29(6): 127-136.
[7]	张梦, 李本银, 刘春增, 吕玉虎, 张成兰, 陈雪青, 曹卫东. 紫云英荚果分层成熟特性及其种子产量研究[J]. 草业学报, 2020, 29(2): 64-72.
[8]	许爱云, 曹兵, 谢云. 干旱风沙区煤炭基地12种草本植物对干旱胁迫的生理生态响应及抗旱性评价[J]. 草业学报, 2020, 29(10): 22-34.
[9]	杨封科, 何宝林, 张国平, 张立功, 高应平. 膜下秸秆还田添加腐解剂对旱地土壤碳氮积累及土壤肥力性状的影响[J]. 草业学报, 2019, 28(9): 67-76.
[10]	李佶恺, 王建丽, 尚晨, 张海玲, 刘杰淋, 陈积山, 潘多锋, 刘凯. 不同藜麦材料在哈尔滨地区的适应性研究[J]. 草业学报, 2019, 28(9): 202-208.
[11]	李亚娇, 马培杰, 吴佳海, 牟琼, 覃涛英, 王晓强, 马宁, 张蓉, 李德芳, 朗永祥, 吴有松, 田应学, 韩永芬. 不同品种青贮玉米与拉巴豆套种对青贮玉米农艺性状及产量的影响[J]. 草业学报, 2019, 28(9): 209-216.
[12]	刘晓, 王博, 朱晓艳, 郭晓洁, 王成章, 李德锋. 21个粮饲兼用型青贮玉米在河南的品种比较试验[J]. 草业学报, 2019, 28(8): 49-60.
[13]	梁国玲, 张永超, 贾志锋, 马祥, 刘文辉. 高寒区不同燕麦品种(系)表型性状和茎秆力学特征与抗倒伏性的关系研究[J]. 草业学报, 2019, 28(4): 58-69.
[14]	闵学阳, 韦兴燚, 刘文献, 张正社, 金小煜, NDAYAMBAZABoniface, 吴洪林, 李昱, 王彦荣. 箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建[J]. 草业学报, 2019, 28(4): 116-128.
[15]	何飞, 张帆, 张铁军, 康俊梅, 龙瑞才, 杨青川, 马春晖. 紫花苜蓿开花性状的遗传特性分析与QTL定位[J]. 草业学报, 2019, 28(2): 112-120.