草业学报 ›› 2022, Vol. 31 ›› Issue (7): 209-219.DOI: 10.11686/cyxb2021188
• 研究论文 • 上一篇
刘思林1(), 陈俊雪1, 杨阳1, 陈中文1, 卢玲玲1, 牟英辉1,2()
收稿日期:
2021-05-07
修回日期:
2021-06-16
出版日期:
2022-07-20
发布日期:
2022-06-01
通讯作者:
牟英辉
作者简介:
E-mail: youhymoon@hotmail.com基金资助:
Si-lin LIU1(), Jun-xue CHEN1, Yang YANG1, Zhong-wen CHEN1, Ling-ling LU1, Ying-hui MU1,2()
Received:
2021-05-07
Revised:
2021-06-16
Online:
2022-07-20
Published:
2022-06-01
Contact:
Ying-hui MU
摘要:
为探讨不同腐解天数及不同浓度梯度紫云英的腐解液对牛筋草种子萌发及二叶期幼苗生长的影响,以牛筋草种子为材料进行发芽试验,测定发芽率等6个生理指标。采用不同浓度的紫云英腐解液对二叶期幼苗进行灌根处理,测定牛筋草叶片的保护酶活性、脯氨酸(Pro)和丙二醛(MDA)含量。结果表明:相同浓度腐解液处理条件下,随着腐解天数的增加,腐解液对牛筋草的抑制作用增强;腐解液对牛筋草的化感作用存在“低促高抑”的现象;当腐解时间超过2 d,腐解液处理浓度达到80%时,能100%抑制牛筋草种子萌发。牛筋草幼苗的超氧化物歧化酶(SOD)活性随着腐解液浓度升高而下降;过氧化物酶(POD)在低浓度腐解液处理时酶活性下降,浓度大于30%时酶活性升高;过氧化氢酶(CAT)活性在30%腐解液浓度内随着浓度升高而升高,80%时活性下降。当腐解液浓度≥2%时,随着腐解液浓度的增加,牛筋草叶片MDA、Pro及过氧化氢(H2O2)含量增加。综上所述,紫云英腐解液对牛筋草种子萌发及幼苗生长具有显著的抑制作用。由此可见,紫云英腐解物具有抑制杂草生长的潜力,研究结果可为发掘紫云英中抗杂草活性物质提供参考。
刘思林, 陈俊雪, 杨阳, 陈中文, 卢玲玲, 牟英辉. 紫云英腐解液对牛筋草种子萌发及幼苗生长的影响[J]. 草业学报, 2022, 31(7): 209-219.
Si-lin LIU, Jun-xue CHEN, Yang YANG, Zhong-wen CHEN, Ling-ling LU, Ying-hui MU. Effects of milkvetch (Astragalus sinicus) decomposition leachates on germination and seedling growth of goosegrass (Eleusine indica)[J]. Acta Prataculturae Sinica, 2022, 31(7): 209-219.
图2 不同浓度及不同腐解天数腐解液对牛筋草种子萌发的影响不同小写字母表示同一腐解时长不同处理浓度在5%水平上差异显著。下同。Different lowercase letters indicate that different concentration treatments for the same decay time differ significantly at the 5% level. The same below.
Fig.2 Effects of different concentrations and different decay days decomposed liquids on the seeds germination of goosegrass
图3 不同浓度及不同腐解天数腐解液对牛筋草幼苗生长的影响
Fig.3 Effects of different concentrations and different decay days decomposed liquids on the seedling growth of goosegrass
腐解天数 Decay days (d) | 浓度 Concentration | 发芽势 Germination potential | 发芽率 Germination rate | 发芽指数 Germination index | 活力指数 Vital index | 株高 Plant height | 鲜重 Fresh weight | 化感隶属累加值 RI accumulate value |
---|---|---|---|---|---|---|---|---|
2 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.42 | 0.36 | 0.41 | 0.43 | 0.05 | 0.31 | 1.98 | |
5% | 0.40 | 0.33 | 0.35 | 0.39 | 0.06 | 0.21 | 1.74 | |
30% | -0.55 | -0.21 | -0.49 | -0.47 | 0.03 | 0.10 | -1.57 | |
80% | -1.00 | -0.94 | -0.98 | -0.99 | -0.26 | -0.64 | -4.80 | |
3 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.20 | 0.19 | 0.20 | 0.34 | 0.18 | 0.30 | 1.39 | |
5% | -0.37 | -0.32 | -0.37 | -0.22 | 0.19 | 0.28 | -0.81 | |
30% | -0.91 | -0.86 | -0.91 | -0.87 | 0.30 | 0.21 | -3.03 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
7 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.00 | -0.07 | -0.10 | 0.08 | 0.17 | 0.38 | 0.46 | |
5% | -0.81 | -0.19 | -0.49 | -0.38 | 0.18 | 0.07 | -1.61 | |
30% | -1.00 | -0.99 | -1.00 | -1.00 | -1.00 | -1.00 | -5.99 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
15 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | -0.98 | -0.48 | -0.79 | -0.77 | 0.09 | 0.01 | -2.91 | |
5% | -1.00 | -0.91 | -0.99 | -0.99 | -0.02 | -1.00 | -4.91 | |
30% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 |
表1 紫云英2~15 d腐解液对牛筋草种子萌发和生长的综合评价
Table 1 Comprehensive evaluation of 2-15 days decomposed liquids on seed germination and growth of goosegrass
腐解天数 Decay days (d) | 浓度 Concentration | 发芽势 Germination potential | 发芽率 Germination rate | 发芽指数 Germination index | 活力指数 Vital index | 株高 Plant height | 鲜重 Fresh weight | 化感隶属累加值 RI accumulate value |
---|---|---|---|---|---|---|---|---|
2 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.42 | 0.36 | 0.41 | 0.43 | 0.05 | 0.31 | 1.98 | |
5% | 0.40 | 0.33 | 0.35 | 0.39 | 0.06 | 0.21 | 1.74 | |
30% | -0.55 | -0.21 | -0.49 | -0.47 | 0.03 | 0.10 | -1.57 | |
80% | -1.00 | -0.94 | -0.98 | -0.99 | -0.26 | -0.64 | -4.80 | |
3 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.20 | 0.19 | 0.20 | 0.34 | 0.18 | 0.30 | 1.39 | |
5% | -0.37 | -0.32 | -0.37 | -0.22 | 0.19 | 0.28 | -0.81 | |
30% | -0.91 | -0.86 | -0.91 | -0.87 | 0.30 | 0.21 | -3.03 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
7 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | 0.00 | -0.07 | -0.10 | 0.08 | 0.17 | 0.38 | 0.46 | |
5% | -0.81 | -0.19 | -0.49 | -0.38 | 0.18 | 0.07 | -1.61 | |
30% | -1.00 | -0.99 | -1.00 | -1.00 | -1.00 | -1.00 | -5.99 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
15 | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2% | -0.98 | -0.48 | -0.79 | -0.77 | 0.09 | 0.01 | -2.91 | |
5% | -1.00 | -0.91 | -0.99 | -0.99 | -0.02 | -1.00 | -4.91 | |
30% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 | |
80% | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -1.00 | -6.00 |
图5 不同浓度15 d腐解液对牛筋草幼苗的影响*,**,***和****分别表示与对照组相比,在P<0.05,P<0.01,P<0.001和P<0.0001水平下差异显著。下同。*, **, ***and **** were significantly different from CK at the level of P<0.05, P<0.01, P<0.001 and P<0.0001, respectively. The same below.
Fig.5 Effects of different concentrations of 15 days decomposed liquid on goosegrass seedlings
图6 不同浓度15 d腐解液对牛筋草幼苗SOD、POD、CAT活性、MDA及Pro含量的影响
Fig. 6 Effects of different concentrations of 15 days decomposed liquid on SOD, POD, CAT activity, MDA and Pro contents of goosegrass seedlings
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