紫花苜蓿WRKY转录因子基因的克隆与亚细胞定位

摘要/Abstract

摘要： WRKY转录因子家族在植物生长发育各个阶段发挥重要作用。本研究运用电子克隆和RT-PCR结合的方法获得1个紫花苜蓿WRKY转录因子家族成员的cDNA序列,命名为MsWRKY56。该序列长1 267 bp,包含1个951 bp的最大开放阅读框,编码317个氨基酸;构建该基因的瞬时表达载体,通过基因枪轰击洋葱表皮的方法对编码蛋白进行亚细胞定位研究,结果表明该基因编码蛋白定位于细胞核,符合转录因子的亚细胞定位特征,为进一步研究该基因编码蛋白的结构和功能奠定了基础。

Abstract: The WRKY transcription factors play important roles in plant growth and development. A cDNA sequence of Medicago sativa WRKY transcription factor gene was isolated by electronic cloning and RT-PCR (reverse transcription polymerase chain reaction), and was named MsWRKY56. The cDNA sequence was 1 267 bp and contained a maximum open reading frame of 951 bp coding for 317 amino acids. Subcellular localization analysis of a construct with the transient expression vector of the MsWRKY56 gene showed that the MsWRKY56 protein was localized in the nucleus and was thus consistent with the general character of subcellular localization of transcription factors. This research forms a basis to further study the structure and function of the gene encoded protein.

中图分类号:

陈婷婷,杨青川,丁旺,康俊梅,张铁军,张新全. 紫花苜蓿WRKY转录因子基因的克隆与亚细胞定位[J]. 草业学报, 2012, 21(4): 159-167.

CHEN Ting-ting, YANG Qing-chuan, DING Wang, KANG Jun-mei, ZHANG Tie-jun, ZHANG Xin-quan. Cloning and subcellular localization of a WRKY transcription factor gene of Medicago sativa [J]. Acta Prataculturae Sinica, 2012, 21(4): 159-167.

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