山西白羊草种质资源遗传多样性的ISSR分析

doi:10.11686/cyxb20140126

摘要/Abstract

摘要： 利用ISSR分子标记技术对山西33个白羊草种质资源进行遗传多样性分析。白羊草种质间遗传变异较稳定,10条ISSR引物共扩增出99条带,多态性比率(P)为52.23%;通过PopGen 32软件计算出:等位基因数(Na)为1.5253±0.5019,有效等位基因数(Ne)为1.2941±0.3665,Nei’s基因多样性(H)为0.1723±0.1983,Shannon信息指数(I)为0.2590±0.2841;通过NTSYSpc 1.20c软件计算出33份材料间的遗传相似系数(GS)为0.8188~0.9801,遗传距离(GD)为0.0200~0.1988。结果表明,33份种质材料居群水平的遗传多样性较低,而自然条件下白羊草进行无融合生殖可能是导致白羊草居群遗传多样性较低的原因之一。

Abstract: The genetic diversities of 33 germplasm resources of Bothriochloa ischaemum in Shanxi Province were analyzed using inter-simple sequence repeat (ISSR) techniques. The genetic diversity among germplasm resources was relatively stable. Ninety-nine bands,of which 52 (52.23%) were polymorphic,were amplified using 10 pairs of ISSR primers. Analyzes by PopGen32 software showed the number of alleles (Na) was 1.5253±0.5019,the effective number of alleles (Ne) was 1.2941±0.3665,Nei’s gene diversity (H) was 0.1723±0.1983,and Shannon’s information index (I) was 0.2590±0.2841; According to the results of NTSYSpc 1.20c,the genetic similarity coefficient (GS) among 33 germplasm materials ranged from 0.8188 to 0.9801,the genetic distance (GD) ranged from 0.0200 to 0.1988. The population genetic diversity of 33 germplasm materials was low: the possible reason could be that the reproductive strategy of B. ischaemum is apomixic under natural conditions.

中图分类号:

S816

李钰莹，董宽虎. 山西白羊草种质资源遗传多样性的ISSR分析[J]. 草业学报, 2014, 23(1): 217-222.

LI Yu-ying,DONG Kuan-hu. ISSR analysis of genetic diversity of germplasm resources of Bothriochloa ischaemum in Shanxi[J]. Acta Prataculturae Sinica, 2014, 23(1): 217-222.

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