低氢氰酸含量高丹草新品系及其亲本的SSR分析

doi:10.11686/cyxb20140127

摘要/Abstract

摘要： 为明确4个低氢氰酸含量的高丹草新品系SLCN-11、SLCN-12、SLCN-13、SLCN-14及其亲本散穗高粱、黑壳苏丹草、白壳苏丹草、红壳苏丹草和棕壳苏丹草在DNA水平上的差异程度,以育成登记的高丹草品种蒙农青饲3号为对照,对其进行了SSR分析。试验从200个高粱SSR引物中筛选出多态性丰富、重复性好的12对引物,利用这些引物对10个材料进行PCR扩增共得到509个多态性位点,多态性百分率达87.30%。每对引物扩增的SSR指纹图清晰、稳定,可作为鉴别4个高丹草新品系及其亲本的分子依据。10个供试材料间的遗传距离(GD)变幅在0.3165~0.6692之间,平均为0.5359;以GD值0.50为基准,将10个材料划分为5类:蒙农青饲3号、散穗高粱、SLCN-11、SLCN-12、SLCN-13为一类;SLCN-14、棕壳苏丹草为一类;白壳苏丹草、黑壳苏丹草、红壳苏丹草各单独为一类。该研究为下一步低氢氰酸含量高丹草新品种育成及登记利用奠定了基础。

Abstract: To identify the difference degree of four new strains of sorghum-sudangrass with low hydrocyanic acid content SLCN-11,SLCN-12,SLCN-13 and SLCN-14,and their parents at a DNA level,we conducted SSR analyses using the variety Mengnong Qingsi No.3 as a control. A total of 12 SSR primer pairs with high polymorphism and good reproducibility were screened from 200 developed sorghum SSR primer pairs. The 10 materials were analyzed using these SSR primers. A total of 509 polymorphic loci were amplified,and the percentage of polymorphic loci was up to 87.30%. Each SSR polymorphic primer pairs could amplify clear and stable bands,which can be used as molecular basis for identifying the four new strains of sorghum-sudangrass. Genetic distance (GD) among 10 materials ranged from 0.3165-0.6692,with an average of using 0.5359; Using the GD value of 0.50 as a threshold,the 10 materials can be clustered into five groups: Mengnong qingsi No.3,loose spike sorghum,SLCN-11,SLCN-12 and SLCN-13; SLCN-14 and brown hull sudangrass; white hull sudangrass; black hull sudangras; red hull sudangrass. This study laid the groundwork for the next step breeding,registration and utilization of new sorghum-sudangrass varieties with low hydrocyanic acid content.

中图分类号:

S816

于卓，谢锐，于肖夏，马艳红，李造哲，纪明妹. 低氢氰酸含量高丹草新品系及其亲本的SSR分析[J]. 草业学报, 2014, 23(1): 223-228.

YU Zhuo,XIE Rui,YU Xiao-xia,MA Yan-hong,LI Zao-zhe,JI Ming-mei. SSR analysis of sorghum-sudangrass new strains with low hydrocyanic acid content[J]. Acta Prataculturae Sinica, 2014, 23(1): 223-228.

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