MADS-box基因家族在蒺藜苜蓿的全基因组分析

doi:10.11686/cyxb20140628

摘要/Abstract

摘要： MADS-box基因家族是植物体内的重要转录因子,它们广泛地调控着植物生长、发育和生殖等过程。本研究以蒺藜苜蓿基因组数据为材料,采用结构域搜索方法,鉴定了138个MADS-box基因。通过序列比对系统进化分析,将它们分成两大类:Ⅰ型(92个)和Ⅱ型(46个)。同时,通过染色体定位分析发现,134个MADS-box基因定位在染色体上,还有4个MADS-box基因定位在尚未完成最终拼接的超长片段上。蒺藜苜蓿的MADS-box基因家族成员之间存在大量的基因重复,特别是Ⅰ型MADS-box基因,通过基因复制,MADS-box基因家族在染色体上形成多个密集的MADS-box基因簇。对蒺藜苜蓿的RNA-seq表达谱数据分析,发现MADS-box基因在心皮组织、花器官等组织中表达量较高,这表明蒺藜苜蓿的MADS-box基因家族广泛地参与苜蓿组织分化、发育以及生殖等过程的调控,这将为进一步揭示MADS-box类转录因子在蒺藜苜蓿中作用机制提供了重要的参考。

Abstract: MADS-box genes encode important transcription factors in plants that are involved in many processes during growth, development and reproduction. A Pfam domain search of the Medicago truncatula genome revealed 138 putative MADS-box genes. These genes were classified into two classes, type I (92 members) and type II (46 members), based on multiple alignment and phylogenetic analysis. There were 134 MADS-box genes present on all chromosomes in M. truncatula, while four were located on the super-contigs. Like Arabidopsis and rice MADS-box genes, those MADS-box genes expanded through gene duplication events in M. truncatula, especially the type I genes. There were many duplicate gene clusters, where MADS-box genes with high degrees of similarity were clustered across the M. truncatula genome. Analysis of RNA-seq data from different tissues in M. truncatula revealed that MADS-box genes are expressed in seedpod, flower and other tissues. This expression pattern suggests that the MADS-box genes play an important role in tissue differentiation, development and reproduction process in M. truncatula.

中图分类号:

S816

张军,宋丽莉,郭东林,郭长虹,束永俊. MADS-box基因家族在蒺藜苜蓿的全基因组分析[J]. 草业学报, 2014, 23(6): 233-241.

ZHANG Jun,SONG Li-li,GUO Dong-lin,GUO Chang-hong,SHU Yong-jun. Genome-wide identification and investigation of the MADS-box gene family in Medicago truncatula[J]. Acta Prataculturae Sinica, 2014, 23(6): 233-241.

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