EBR对NaCl胁迫下苜蓿属植物离子吸收和分配的影响

doi:10.11686/cyxb2017413

摘要/Abstract

摘要： 为探究苜蓿属植物的抗盐机理及外源油菜素内酯(EBR)对苜蓿属植物抗盐性的影响,选取蒺藜苜蓿、紫花苜蓿和金花菜3种苜蓿属植物为实验材料,研究NaCl胁迫及NaCl胁迫下喷施EBR对苜蓿属植物生长,体内无机离子吸收、分配及运输的影响。结果表明:1)NaCl胁迫会抑制苜蓿属植物的生长,影响植物体内K⁺、Ca²⁺、Mg²⁺离子的分配和运输。2)EBR能有效缓解NaCl胁迫对植物生长的抑制作用,维持植物体内的离子平衡,从而提高植物的耐盐性。3)紫花苜蓿耐盐性较强,蒺藜苜蓿耐盐性较弱。4)在盐胁迫条件下,紫花苜蓿根部抑制对Na⁺的吸收,减少离子毒害作用;蒺藜苜蓿将根中的Na⁺向茎和叶中运输,通过叶片的脱落,维持生长点的正常生长。

关键词: 蒺藜苜蓿, 紫花苜蓿, 金花菜, 盐胁迫, 离子代谢, 2, 4-表油菜素内酯(EBR)

Abstract: The aim of this study was to explore the mechanism of salt tolerance of Medicago species and the effect of exogenous brassinolide on salt tolerance of Medicago species. NaCl stress and the effects of epibrassinolide (EBR) on biomass, uptake, distribution and transport of Medicago species were studied under NaCl stress. Medicago truncatula, M. polymorphal and M. polymorpha were selected as experimental materials. The results show that NaCl stress inhibited the growth of Medicago plants and affect the distribution of K⁺, Ca²⁺, Mg²⁺ in plants. EBR effectively relieved the inhibitory effect of NaCl stress on plant growth, maintaining ion balance in plants and improving tolerance to salt. Salt tolerance in M. sativa was strong whereas tolerance in M. truncatula was poor. Under salt stress, the roots of M. sativa inhibited the uptake of Na⁺ to reduce the toxicity effects. M. truncatula transported Na⁺ from roots to the stem and leaves.

Key words: Medicago truncatula, Medicago sativa, Medicago polymorpha, salt stress, ion metabolism, 2, 4-epibrassinolide

王小山, 季晓敏, 刘隆阳, 纪冰沁, 田银芳. EBR对NaCl胁迫下苜蓿属植物离子吸收和分配的影响[J]. 草业学报, 2018, 27(9): 110-119.

WANG Xiao-shan, JI Xiao-min, LIU Long-yang, JI Bing-qin, TIAN Yin-fang. Effects of epibrassinolide on ion absorption and distribution in Medicago species under NaCl stress[J]. Acta Prataculturae Sinica, 2018, 27(9): 110-119.

参考文献

[1] Guna Er·A Khan, Zhang X F, Zhao Y. Effects of salt stress and alkali stress on germination and growth of wheat-wheatgrass earliest young seedling. Seed, 2010, 29(9): 52-55.
贾娜尔·阿汗, 张相锋, 赵玉. 盐碱胁迫对小冰麦种子萌发和早期幼苗生长的影响. 种子, 2010, 29(9): 52-55.
[2] Gui Z, Gao J M, Yuan Q H, et al. Correlations among characters of alfalfa in germination period under alkaline stress. Journal of Anhui Agricultural Sciences, 2011, 39(3): 1469-1471.
桂枝, 高建明, 袁庆华, 等. 碱胁迫下苜蓿发芽期性状的相关性研究. 安徽农业科学, 2011, 39(3): 1469-1471.
[3] Zhang W Y.The harnessing measures for improvement and utilization of the salinized soil in the coastal areas of Jiangsu province. Journal of Agricultural Resources and Regional Planning, 2000, 21(5): 43-45.
张文渊. 江苏沿海地区盐渍土壤改良利用的治理措施. 中国农业资源与区划, 2000, 21(5): 43-45.
[4] Chen W X.The role of legumes-root nodule bacteria nitrogen fixing system in development of west area of China. Acta Agrestia Sinica, 2004, 12(1): 1-2.
陈文新. 豆科植物根瘤菌-固氮体系在西部大开发中的作用. 草地学报, 2004, 12(1): 1-2.
[5] Shi Y C.Extricate ourselves from the dilemma of desertification control and return of reclaimed farmland (to afforested area). Acta Agrestia Sinica, 2004, 12(2): 83-86.
石元春. 走出治沙与退耕中的误区. 草地学报, 2004, 12(2): 83-86.
[6] Wang W B, Jin R X, Deng X P, et al. Physiological and biological responses of alfalfa shoots and roots to salt stress. Journal of Northwest A & F University (Natural Science Edition), 2009, 37(5): 217-223.
王文斌, 金润熙, 邓西平, 等. 苜蓿幼苗芽、根器官对盐胁迫的生理生化响应. 西北农林科技大学学报(自然科学版), 2009, 37(5): 217-223.
[7] Hong F Z.Alfalfa science. Beijing: China Agricultural Publishing House, 2009.
洪绂曾. 苜蓿科学. 北京: 中国农业出版社,2009.
[8] Qin F M, Zhang H X, Wu H, et al. Effects of salt stress on germination and seedling growth of Medicago falcata. Acta Prataculturae Sinica, 2010, 19(1): 71-7.
秦峰梅, 张红香, 武讳, 等. 盐胁迫对黄花苜蓿发芽及幼苗生长的影响. 草业学报, 2010, 19(1): 71-78.
[9] Bckki A, Trinchant J C, Rigaud J.Nitrogen fixation(C₂H₅ reduction) by Medicago nodules and bacteroids under sodium chloride stress. Physiologia Plantarum, 1987, 71: 61-67.
[10] Liu J L, Yun T Y, Cao J M, et al. Effect of acid treatment on seed germination of Medicago truncatula gaertn. Seed, 2008, 27(9): 87-88.
刘建利,云天运,曹君迈,等. 酸处理对蒺藜状苜蓿种子萌发的影响. 种子,2008, 27(9): 87-88.
[11] Li Z W, Ma T Y, Liang G T, et al. Photosynthesis in leaves of Medicago truncatula under salt stress. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(10): 2070-2077.
李紫薇, 马天意, 梁国婷, 等. 蒺藜苜蓿叶片光合作用对盐胁迫的响应. 西北植物学报, 2014, 34(10): 2070-2077.
[12] Shi T S.Valuable sedge. Farmhouse Getting Rich, 2011, (20): 14-15.
石庭山. 身价不菲的小秧草. 农家致富, 2011, (20): 14-15.
[13] Wang Z Q, Zhu S Q, Yu R P, et al.Chinese saline soil. Beijing: Science Press, 1993.
王遵亲, 祝寿泉, 俞仁培, 等. 中国盐渍土. 北京: 科学出版社, 1993.
[14] Zhang L Q.The affection of brassinolide on the physiological indexes of tomato seedings. Northern Horticulture, 2013, (1): 1-3.
张林青. 油菜素内酯对盐胁迫下番茄幼苗生理指标的影响. 北方园艺, 2013, (1): 1-3.
[15] Bajguz A, Hayat S.Effects of brassinosteroids on the responses to environmental stresses. Plant Physiology and Biochcmistry, 2009, 17: 1-8.
[16] Wang H H, Li K R, Hou H W.Research progress of plant stress-resistance promoting related to brassinolides. Agricultural Research in the Arid Areas, 2005, 23(3): 213-219.
王红红, 李凯荣, 侯华伟. 油菜素内酯提高植物抗逆性的研究进展. 干旱地区农业研究, 2005, 23(3): 213-219.
[17] Zhang K, Zhang D Y, Wang L, et al. Study on the ionic absorption and transport in Salicornia europaea L. growing in natural habitats in Xinjiang. Arid Zone Research, 2007, 2(4): 480-486.
张科, 张道远, 王雷, 等. 自然生境下盐角草的离子吸收—运输特征. 干旱区研究, 2007, 2(4): 480-486.
[18] Wang S M.Effects of salt stress on the characteristics of ion absorption and distribution in Puccinellia tenuiflora. Acta Agrestia Sinica, 1996, 4(3): 186-193.
王锁民. 不同程度盐胁迫对碱茅离子吸收与分配的影响. 草地学报, 1996, 4(3): 186-193.
[19] Cai Q S.Plant physiology. Beijing: China Agricultural University Press, 2014.
蔡庆生. 植物生理学. 北京: 中国农业大学出版社, 2014.
[20] Lu X M, Sun J, Guo S R, et al. Effects of brassinolide on the leaf mitochondria and chloroplast ultrastructure and photosynthesis of cucumber seedlings under hypoxia stress. Acta Horticulturae Sinica, 2012, 39(5): 888-896.
陆晓民,孙锦,郭世荣,等. 油菜素内酯对低氧胁迫下黄瓜幼苗叶片线粒体、叶绿体超微结构及光合的影响. 园艺学报,2012,39(5): 888-896.