转多抗基因新疆大叶苜蓿光合生理特征对土壤水分变化的响应

doi:10.11686/cyxb2017504

草业学报 ›› 2018, Vol. 27 ›› Issue (11): 95-105.DOI: 10.11686/cyxb2017504

转多抗基因新疆大叶苜蓿光合生理特征对土壤水分变化的响应

李敏¹, 苏国霞¹, 熊沛枫¹, 康继月², 郭尚洙³, 王智^{1, 2, *}, 徐炳成^{1, 2, *}

1.西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100;
2.中国科学院水利部水土保持研究所,陕西杨凌 712100;
3.韩国生命工学研究院植物系统工程研究中心,韩国大田 305-806

收稿日期:2017-12-13 修回日期:2018-03-28 出版日期:2018-11-20 发布日期:2018-11-20
通讯作者: wangzhi712@nwafu.edu.cn, Bcxu@ms.iswc.ac.cn
作者简介:李敏(1991-),女,山西吕梁人,在读硕士。E-mail: 549024295@qq.com
基金资助:
国家自然科学基金(31700335),西北农林科技大学博士科研启动费(2452015341)和韩国生命工学研究院启动项目(KRIBB Initiative Program)资助

Responses of photosynthetic physiological traits in transgenic alfalfa expressing multi-functional genes during soil moisture change

LI Min¹, SU Guo-xia¹, XIONG Pei-feng¹, KANG Ji-yue², KWAK Sang-soo³, WANG Zhi^{1, 2, *}, XU Bing-cheng^{1, 2, *}

1.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China;
2.Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, China;
3.Plant Systems Engineering Research Center, Korea Research Institute of Biotechnology and Biotechnology, Daejeon 305-806, Korea

Received:2017-12-13 Revised:2018-03-28 Online:2018-11-20 Published:2018-11-20

摘要/Abstract

摘要： 以新疆大叶苜蓿及其为母本的4种转基因株系(SN:转AtNDPK2基因;SC:转codA基因;SOR:转IbOr基因;SAF:转AtABF3基因)为研究材料,通过盆栽控制试验,比较研究了不同土壤水分条件下始花期各株系苜蓿的光合和荧光参数特征。结果表明,随土壤含水量从80% FC(田间持水量)降至40% FC,各株系叶片净光合速率(P_n)、蒸腾速率(T_r)和气孔导度(G_s)均呈下降趋势,叶片瞬时水分利用效率(WUE_i)呈上升趋势。在40% FC时,各转基因株系P_n值(11.19~21.58 μmol·m^-2·s^-1)均显著(P<0.05)大于非转基因株系(6.06 μmol·m^-2·s^-1),各株系间WUE_i无差异但以转AtABF3基因株系最大(3.22 μmol·mmol^-1)。土壤水分降低过程中,各株系叶片初始荧光(F_o),最大光化学效率(F_v/F_m)和光化学淬灭系数(qP)值总体呈下降趋势,实际光化学效率(Φ_PSⅡ)和表观电子传递效率(ETR)值呈先上升后下降,而非光化学淬灭系数(NPQ)值整体呈上升趋势。转基因株系qP值出现显著下降时的土壤水分含量(50% FC)低于非转基因(70% FC)苜蓿。在40% FC时,转基因株系F_v/F_m(0.78~0.82)值显著高于非转基因苜蓿(0.58)。总体表明,转多抗基因均不同程度地提高水分胁迫条件下新疆大叶苜蓿的光合性能,其中转codA基因株系可维持较高光合速率,转IbOr基因株系能维持较高光能利用能力,转AtABF3基因株系能够高效利用水分,转AtNDPK2基因株系总生物量最高。

关键词: 多抗基因, 紫花苜蓿, 干旱胁迫, 光合特征, 荧光参数

Abstract: The photosynthetic and fluorescence characteristics of alfalfa (cv. Xinjiang Daye) and four transgenic lines (SN: transgenic AtNDPK2, SC: transgenic codA, SOR: transgenic IbOr, SAF: transgenic AtABF3) at the early flowering stage were investigated and compared under different soil moisture conditions in a pot experiment. Results showed that the net photosynthetic rate (P_n), transpiration rate (T_r) and stomatal conductance (G_s) of all lines showed a decreasing trendas soil moisture decreased from 80% FC (field capacity) to 40% FC, while instantaneous water use efficiency (WUE_i) exhibited an increasing trend. At 40% FC, P_n values of transgenic lines were 11.19-21.58 μmol·m^-2·s^-1, significantly (P<0.05) higher than that of non-transgenic (NT) plants (6.06 μmol·m^-2·s^-1). There were no differences in WUE_i among all alfalfa lines and the SAF line had the highest value (3.22 μmol·mmol^-1). As soil moisture decreased, the initial fluorescence (F_o), maximum photochemical efficiency (F_v/F_m) and photochemical quenching coefficient (qP) values exhibited declining trends, and actual photochemical efficiency (Φ_PSⅡ) and apparent electron transfer efficiency (ETR) values initially increased and then declined, while non-photochemical quenching coefficient (NPQ) values showed an increasing trend.The qP values of transgenic lines decreased significantly at 50% FC, while that of NT was 70% FC. Under 40% FC, F_v/F_m values of transgenic lines were 0.78-0.82, and were significantly higher than that of NT (0.58). This suggests that the expression of multi-functional genes could improve photosynthetic properties of Xinjiang Daye to some extent and that transgenic codA is able to sustain a high photosynthetic rate, transgenic IbOr line can maintain higher light use ability, transgenic AtABF3 is able to utilize water more efficiently, while transgenic AtNDPK2 had the highest total biomass production with decreasing soil moisture.

Key words: multi-functional gene, alfalfa, drought stress, photosynthetic characteristics, fluorescence parameter

李敏, 苏国霞, 熊沛枫, 康继月, 郭尚洙, 王智, 徐炳成. 转多抗基因新疆大叶苜蓿光合生理特征对土壤水分变化的响应[J]. 草业学报, 2018, 27(11): 95-105.

LI Min, SU Guo-xia, XIONG Pei-feng, KANG Ji-yue, KWAK Sang-soo, WANG Zhi, XU Bing-cheng. Responses of photosynthetic physiological traits in transgenic alfalfa expressing multi-functional genes during soil moisture change[J]. Acta Prataculturae Sinica, 2018, 27(11): 95-105.

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转多抗基因新疆大叶苜蓿光合生理特征对土壤水分变化的响应

Responses of photosynthetic physiological traits in transgenic alfalfa expressing multi-functional genes during soil moisture change

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