新疆不同生境狗牙根种质抗旱性综合评价

doi:10.11686/cyxb2019486

摘要/Abstract

摘要： 以2份国外品种(对照)和41份来自不同生境的新疆狗牙根种质为供试材料,设置干旱处理和对照处理,在30 d干旱处理后测定叶绿素含量、绿叶率、相对电导率、叶片相对含水量、脯氨酸含量、丙二醛含量、生长速度和地上生物量的基础上,采用相关分析、主成分分析构建综合评价指标,进一步结合隶属函数分析、聚类分析、灰色关联度分析及判别分析方法对其抗旱性进行综合评价,筛选抗旱鉴定关键指标,明确种质抗旱性与其生境气候带间关系。结果表明,干旱胁迫后供试种质的相对电导率、脯氨酸及丙二醛含量呈显著增加,叶片相对含水量、叶绿素含量、地上生物量、生长速度和绿叶率显著降低,各指标对抗旱性的响应存在一定差异。主成分分析将8个指标转换为6个综合指标,代表种质抗旱性87.34%的数据信息;依据抗旱性度量值(D)、综合抗旱系数(CDC)、加权抗旱系数(WDC)的隶属函数分析及聚类分析结果,认为C₁₁₈、C₁₃₈、C₂₄抗旱性较强,C₁₀、C₁₃₄、C₃₂抗旱性较弱。灰色关联度分析认为相对电导率、叶绿素含量及丙二醛含量可作为狗牙根种质抗旱评价的关键指标,且判别分析认为新疆狗牙根种质抗旱性与其生境气候带间无明显相关性。综合分析发现C₁₁₈、C₁₃₈、C₂₄等种质抗旱性优于Tifway,其为狗牙根抗旱新品种选育提供可用优异种质,也为进一步研究狗牙根抗旱生理及分子机制提供可用材料。

关键词: 干旱胁迫, 狗牙根, 抗旱指标, 综合评价

Abstract: This research used two commercial varieties of bermudagrass (Cynodon dactylon) as control and evaluated performance of 41 germplasm accessions of bermudagrass collected from different habitats in Xinjiang province under drought and control treatments. After 30 days of drought treatment, the chlorophyll content, green leaf proportion, relative conductivity, leaf relative water content, proline content, malondialdehyde content, speed of growth and aboveground biomass under natural drought stress were measured. Based on these data, correlation analysis and principal component analysis were performed to construct multi-trait evaluation indicators, and combined with membership function analysis, cluster analysis, grey correlation analysis and discriminant analysis methods to comprehensively evaluate multiple trait expression linked to drought resistance, develop key indexes for identification of drought resistance, and clarify the relationship between drought resistance of germplasm accessions and their habitat climate zone. It was found that the relative conductivity, proline and malondialdehyde content of the tested germplasm significantly increased under drought stress, and the relative water content, chlorophyll content, aboveground biomass, speed of growth and green leaf rate significantly decreased. There were differences in response to drought stress between all indices. The eight measured traits were reduced by principal component analysis to six independent scores, representing 87.34% of the total data variation. The result of membership function analysis based on drought resistance comprehensive evaluation value (D), comprehensive drought resistance coefficient (CDC), and weighted drought resistance coefficient (WDC) analysis and cluster analysis showed that accessions designated C₁₁₈, C₁₃₈ and C₂₄ had strong drought resistance, while accessions designated C₁₀, C₁₃₄ and C₃₂ had weaker drought resistance. The grey correlation analysis indicated that relative conductivity, leaf chlorophyll content and malondialdehyde content could be used as key indexes for drought resistance evaluation of bermudagrass germplasm, and discriminant analysis indicated that there was no significant correlation between drought resistance of Xinjiang Bermudagrass germplasm and its habitat climate zone. The comprehensive analysis showed that the drought resistance of C₁₁₈, C₁₃₈, C₂₄ and other germplasm acessions was better than that of the commercial variety Tifway. Hence this research has provided excellent germplasm for breeding new bermudagrass varieties with good drought resistance, and provided materials for further study of physiological and molecular mechanisms of bermudagrass drought resistance.

Key words: drought stress, Cynodon dactylon, drought resistance index, comprehensive evaluation

曾令霜, 李培英, 孙晓梵, 孙宗玖. 新疆不同生境狗牙根种质抗旱性综合评价[J]. 草业学报, 2020, 29(8): 155-169.

ZENG Ling-shuang, LI Pei-ying, SUN Xiao-fan, SUN Zong-jiu. A multi-trait evaluation of drought resistance of bermudagrass (Cynodon dactylon) germplasm from different habitats in Xinjiang province[J]. Acta Prataculturae Sinica, 2020, 29(8): 155-169.

参考文献

[1] Vahid M, Mostafa V, Reza S, et al. Evaluation of end drought tolerance of 12 wheat genotypes by stress indices. Middle-East Journal of Scientific Research, 2011, 7(2): 241-247.
[2] Xu L X.Mechanism involved in drought response and post-drought recovery of Kentucky bluegrass. Beijing: Beijing Forestry University, 2011.
许立新. 草地早熟禾适应干旱以及干旱后复水恢复机理研究. 北京: 北京林业大学, 2011.
[3] Cui Y, Wang J S, Wang X C.Phenotypic and genotypic diversity for drought tolerance among and within perennial ryegrass accessions. HortScience, 2015, 50(8): 1148-1154.
[4] Koski V.Breeding plans in case of global warming. Euphytica, 1996, 92(1): 235-239.
[5] Wilkins P W.Breeding perennial ryegrass for agriculture. Euphytica, 1991, 52(3): 201-214.
[6] Hu H, Cao Y, Wang Y.Impact of water stress on seed germination and seedling growth of Cynodon dactylon. Pratacultural Science, 2013, 30(1): 63-68.
胡红, 曹昀, 王颖. 水分胁迫对狗牙根种子萌发及幼苗生长的影响. 草业科学, 2013, 30(1): 63-68.
[7] Qian Y, Fry J D.Water relations and drought tolerance of four turfgrasses. Journal of the American Society for Horticultural Science, 1997, 122(1): 129-133.
[8] Zhou Y, Lambrides C, Kearns R, et al. Selecting for drought tolerance among Australian green couch grasses (Cynodon spp.). Crop and Pasture Science, 2009, 60(12): 1175-1183.
[9] Zhou Y, Lambrides C, Fukai S.Drought resistance of bermudagrass (Cynodon spp.) ecotypes collected from different climatic zones. Environmental and Experimental Botany, 2013, 85: 22-29.
[10] Sun J, Luo H, Fu J, et al. Classification of genetic variation for drought tolerance in tall fescue using physiological traits and molecular markers. Crop Science, 2013, 53(2): 647-654.
[11] Beard J B, Sifers S I.Genetic diversity in dehydration avoidance and drought resistance within the Cynodon and Zoysia species. International Turfgrass Society Research Journal, 1997, 8: 603-610.
[12] Abulaiti, Shi D S, Yang G, et al. Preliminary research report on the native Cynodon dactylon in Xinjiang. Journal of Xinjiang Agricultural University, 1998, 21(2): 124-127.
阿不来提, 石定燧, 杨光, 等. 新疆野生狗牙根研究初报. 新疆农业大学学报, 1998, 21(2): 124-127.
[13] Duan M M.Evaluation of drought resistance and physiological response of Xingjiang wild bermudagrass germplasm. Urumqi: Xinjiang Agricultural University, 2016.
段敏敏. 新疆野生狗牙根种质抗旱性鉴定及抗旱生理的研究. 乌鲁木齐: 新疆农业大学, 2016.
[14] Li Y, Hou X Y, Wu Z N, et al. Comprehensive evaluation on drought resistance of Leymus chinensis germplasm resources. Chinese Journal of Grassland, 2019, 41(1): 75-82.
李怡, 侯向阳, 武自念, 等. 羊草种质资源抗旱性综合评价. 中国草地学报, 2019, 41(1): 75-82.
[15] Bai S Q, Yan J J, Zeng Y, et al. Comprehensive evaluation on drought-resistance of 9 native Elymus sibiricus germplasms resources. Prataculture and Animal Husbandry, 2013, (2): 1-5, 9.
白史且, 鄢家俊, 曾怡, 等. 9份野生老芒麦种质资源抗旱性综合评价. 草业与畜牧, 2013, (2): 1-5, 9.
[16] Tan Y X, Liu R T, Wang X G, et al. A study on the evapotranspiration and drought resistance of 12 buffalograss [Buchloe dactyloides (Nutt.) Engelm] accessions. Chinese Journal of Grassland, 2010, 32(1): 40-47.
谭玉霞, 刘荣堂, 王显国, 等. 12份野牛草材料蒸散量及抗旱性的研究. 中国草地学报, 2010, 32(1): 40-47.
[17] Qi J, Yan W H, Xu C L, et al. Comprehensive evaluation on adaptability of the wild Elymus L. germplasm in arid and semi-arid region. Chinese Journal of Grassland, 2013, 35(4): 40-46.
祁娟, 闫伟红, 徐长林, 等. 披碱草属野生种质材料在干旱与半干旱区适应性评价. 中国草地学报, 2013, 35(4): 40-46.
[18] Sun L, Liu G D.Comprehensive evaluation on drought resistance of ten Cynodon dactylon varieties. Chinese Journal of Tropical Agriculture, 2011, 31(5): 61-64, 83.
孙莉, 刘国道. 10份狗牙根种质的抗旱性综合评价. 热带农业科学, 2011, 31(5): 61-64, 83.
[19] Duan M M, Li P Y, Sun Z J, et al. Evaluation of drought resistance of 50 Cynodon dactylon germplasms at germinating stage under PEG stress. Chinese Journal of Grassland, 2015, 37(4): 27-34.
段敏敏, 李培英, 孙宗玖, 等. PEG胁迫下50份新疆狗牙根种质芽期抗旱性鉴定. 中国草地学报, 2015, 37(4): 27-34.
[20] Zhang G Z.Study and evaluation on drought resistance of native bermudagrass in Sichuan. Chengdu: Sichuan Agricultural University, 2005.
张国珍. 四川野生狗牙根抗旱性研究及评价. 成都: 四川农业大学, 2005.
[21] Xu D Y.Xinjiang agricultural climatic resources and divisions. Beijing: China Meteorological Press, 1989.
徐德源. 新疆农业气候资源及区划. 北京: 气象出版社, 1989.
[22] Li H S.Principle and technology of plant biochemistry experiment. Beijing: High Education Press, 2006.
李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2006.
[23] Tang Z C.Modern plant physiology experiment. Beijing: Science Press, 1999.
汤章成. 现代植物生理学实验指南. 北京: 科学出版社, 1999.
[24] Karcher D E, Richardson M D, Hignight K, et al. Drought tolerance of tall fescue populations selected for high root/shoot ratios and summer survival. Crop Science, 2008, 48(2): 771-777.
[25] Cai Q S.Plant physiology experiment. Beijing: China Agricultural University Press, 2012.
蔡庆生. 植物生理学实验. 北京: 中国农业大学出版社, 2012.
[26] Zhang D L, Liu J N, Wang Y Q, et al. Physiological response and comprehensive evaluation on the drought resistance of germplasm resources at seedling stage in Festuca. Journal of Shanxi Agricultural Sciences, 2010, 38(10): 20-25.
张东玲, 刘建宁, 王运琦, 等. 羊茅种质资源苗期抗旱生理响应及综合评价. 山西农业科学, 2010, 38(10): 20-25.
[27] Wang C, Zhou L B, Zhang G B, et al. Identification and indices screening of drought resistance in job’s tears germplasm resources at germination stage. Journal of Plant Genetic Resources, 2017, 18(5): 846-859.
汪灿, 周棱波, 张国兵, 等. 薏苡种质资源萌发期抗旱性鉴定及抗旱指标筛选. 植物遗传资源学报, 2017, 18(5): 846-859.
[28] Wang C, Zhou L B, Zhang G B, et al. Drought resistance identification and drought resistance indices screening of job’s tears (Coxi lacryma-jobi L.) germplasm resources at seeding stage. Scientia Agricultural Sinica, 2017, 50(15): 2872-2887.
汪灿, 周棱波, 张国兵, 等. 薏苡种质资源苗期抗旱性鉴定及抗旱指标筛选. 中国农业科学, 2017, 50(15): 2872-2887.
[29] Liu G H, Chen Q J, Wu P H, et al. Screening and comprehensive evaluation of drought resistance indices of cotton at blossing and bollforming stages. Journal of Plant Genetic Resources, 2016, 17(1): 53-62, 69.
刘光辉, 陈全家, 吴鹏昊, 等. 棉花花铃期抗旱性综合评价及指标筛选. 植物遗传资源学报, 2016, 17(1): 53-62, 69.
[30] Lu G H, An H R.Advances in studies on methods and indexes for identification of crop drought resistance. Journal of Shanxi Agricultural Sciences, 1999, 27(4): 39-43.
路贵和, 安海润. 作物抗旱性鉴定方法与指标研究进展. 山西农业科学, 1999, 27(4): 39-43.
[31] Azarinasrabad A, Mousavinik S M, Galavi M, et al. Evaluation of water stress on yield, its components and some physiological traits at different growth stages in grain sorghum genotypes. Notulae Scientia Biologicae, 2016, 8(2): 204-210.
[32] Sun H B, Ren R F, Guo F, et al. Research on morphological and physiological responses of Monarda didyma seedlings to drought stress. Shanxi Agricultural University (Natural Science Edition), 2018, 38(4): 65-73.
孙海博, 任瑞芬, 郭芳, 等. 干旱胁迫下美国薄荷幼苗形态与生理特性研究. 山西农业大学学报(自然科学版), 2018, 38(4): 65-73.
[33] Li M, Wang G X.Effect of drought stress on activities of cell defense enzymes and lipid peroxidation in Glycyrrhiza uralensis seedlings. Acta Ecologica Sinica, 2002, 22(4): 503-507.
李明, 王根轩. 干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响. 生态学报, 2002, 22(4): 503-507.
[34] Niu D W, Ma N, Fang Z Y, et al. Physiological study of drought tolerant Nassella tenuissima under water. Pratacultural Science, 2018, 35(3): 581-589.
牛东伟, 马男, 房卓研, 等. 水分胁迫下墨西哥羽毛草耐旱生理. 草业科学, 2018, 35(3): 581-589.
[35] Guo Y P, Mi F G, Yan L J, et al. Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties. Acta Prataculturae Sinica, 2014, 23(4): 220-228.
郭郁频, 米福贵, 闫利军, 等. 不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价. 草业学报, 2014, 23(4): 220-228.
[36] Zhu H S, Wang B P, Dong X Y, et al. Effects of drought stress and rewatering on the growth and physiological characteristics of Shanxi wild Poa pratensis seedlings. Acta Pratacultura Sinica, 2015, 24(7): 79-88.
朱慧森, 王保平, 董晓燕, 等. 干旱及复水对山西野生草地早熟禾幼苗生长与生理特性的影响. 草业学报, 2015, 24(7): 79-88.
[37] Yi J, Gu A L, Jia G H, et al. Studies on the drought hardiness in seedling of Leymus Hochst. Journal of Arid Land Resources and Environment, 2001, 15(5): 47-50.
易津, 谷安琳, 贾光宏, 等. 赖草属牧草幼苗耐旱性生理基础的研究. 干旱区资源与环境, 2001, 15(5): 47-50.
[38] Wang P, Chen J H, Wang P, et al. Status of research into the abiotic stress tolerance of Elymus specie. Acta Prataculturae Sinica, 2019, 28(5): 156-162.
王沛, 陈玖红, 王平, 等. 披碱草属植物抗逆性研究现状和存在的问题. 草业学报, 2019, 28(5): 151-162.
[39] David L D M, John K M, James H R, et al. Physiological genomics of response to soil drying in diverse Arabidopsis accessions. The Plant Cell, 2012, 24(3): 893-914.
[40] Xu X W, Luo S B, Lei J J, et al. Multivariate statistical methods and their application in crops environment stress. Chinese Agricultural Science Bulletin, 2009, 25(12): 267-273.
徐小万, 罗少波, 雷建军, 等. 多变量统计方法及其在农作物环境胁迫研究中的应用. 中国农学通报, 2009, 25(12): 267-273.
[41] Liu Y M, Zhang X Z, Tran H, et al. Assessment of drought tolerance of 49 switchgrass (Panicum virgatum) genotypes using physiological and morphological parameters. Biotechnology for Biofuels, 2015, 8(1): 152.