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草业学报 ›› 2018, Vol. 27 ›› Issue (11): 171-178.DOI: 10.11686/cyxb2017512

• 研究论文 • 上一篇    下一篇

干旱胁迫下BR与N、P、K配合对羊草生长及抗旱性的影响

宋吉轩1, 2, 吕俊2, 宗学凤2, 何秀娟2, 徐宇2, 吴潇2, 王三根2, *   

  1. 1.贵州省农业科学院生物技术研究所,贵州 贵阳 550006;
    2.西南大学农学与生物科技学院,重庆400715
  • 收稿日期:2017-12-18 修回日期:2018-02-03 出版日期:2018-11-20 发布日期:2018-11-20
  • 通讯作者: wangsg@swu.edu.cn
  • 作者简介:宋吉轩(1978-),男,贵州思南人,副研究员,在读博士。E-mail: songjx1025@163.com
  • 基金资助:
    国家重点基础研究发展计划973项目(2014CB138806),中国111计划作物种质资源利用创新基地建设项目(104510-205001)和贵州省第五批创新人才基地建设(黔人领发[2016]22号)资助

Effects of brassinolide and N, P, K fertiliser on growth of Leymus chinensis under drought stress

SONG Ji-xuan1, 2, LÜ Jun2, ZONG Xue-feng2, HE Xiu-juan2, XU Yu2, WU Xiao2, WANG San-gen2, *   

  1. 1.Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2.College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2017-12-18 Revised:2018-02-03 Online:2018-11-20 Published:2018-11-20

摘要: 受气候变化如干旱胁迫作为危害羊草主要的环境因子之一,对其生产力的提高产生了严重的影响。通过盆栽人工控水法,研究在正常(土壤相对含水量为80%)和干旱(土壤相对含水量为50%)条件下油菜素内酯(BR)与氮、磷、钾(N、P、K)配合处理对羊草幼苗生长及其抗旱性的影响。结果表明:适当浓度的BR可以减轻干旱胁迫对植株的伤害,而BR与N、P、K配合(1%尿素+1%磷酸二氢钾+0.10 mg·L-1 BR)则可进一步促进植株生长和营养物质的积累,提高羊草的抗旱性,效果更好。这种效应与其调控植株生长,提升渗透调节能力,降低膜脂的过氧化作用,增强抗氧化酶活性等生理特性密切相关。配合处理下羊草的株高、鲜重、干重和根系活力比干旱对照(CK2)分别增加28.96%、62.46%、45.21%和19.08%,N、P、K含量分别增加20.74%、236.84%和42.31%,营养代谢相关酶硝酸还原酶(NR)、酸性磷酸酶(ACP)和苹果酸脱氢酶(MD)活性发生改变,脯氨酸、可溶性蛋白和可溶性糖分别增加57.20%、22.82%和5.94%,而丙二醛和叶片电导率分别降低37.42%和55.79%,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)分别增加158.40%、38.15%、32.97%、30.00%和28.74%。

关键词: 羊草, 油菜素内酯(BR), 干旱胁迫, 渗透调节, 酶特性

Abstract: Climate change is one of the main environmental factors with the potential to influence the productivity of Leymus chinensis. In this paper, potted plants were used to study the effects of brassinolide (BR) and nitrogen, phosphorus and potassium (N, P, K) on the growth and drought resistance of L. chinensis under normal (soil water content 80% of field capacity) and drought (soil water content 50% of field capacity) conditions. The results showed that appropriate BR concentration could alleviate plant injury under drought stress while the combination of BR and N, P, K (1% urea+1% potassium dihydrogen phosphate+0.10 mg·L-1 BR) could promote plant growth and nutrient accumulation, further improving drought resistance in L. chinensis. This was closely related to key physiological characteristics including regulation of plant growth, enhanced osmotic adjustment, reduced membrane lipid peroxidation and enhanced antioxidant enzyme activity. Plant height, fresh weight, dry weight and root vigour increased by 28.96%, 62.46%, 45.21% and 19.08% in the BR treated plants and with N, P, K by 20.74%, 236.84% and 42.31% respectively compared with the CK. The activities of nitrate reductase (NR), acid phosphatase (ACP) and malate dehydrogenase (MD) changed with proline content; soluble protein and soluble sugar increased by 57.20%, 22.82% and 5.94% respectively. Malondialdehyde conductivity and leaf permeability were reduced by 37.42% and 55.79% respectively, while superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione reductase (GR) and ascorbate peroxidase (APX) activity increased (158.40%, 38.15%, 32.97%, 30.00% and 28.74% respectively).

Key words: Leymus chinensis, brassinolide (BR), drought stress, osmotic adjustment, enzyme characteristics