干旱胁迫下BR与N、P、K配合对羊草生长及抗旱性的影响

doi:10.11686/cyxb2017512

摘要/Abstract

摘要： 受气候变化如干旱胁迫作为危害羊草主要的环境因子之一,对其生产力的提高产生了严重的影响。通过盆栽人工控水法,研究在正常(土壤相对含水量为80%)和干旱(土壤相对含水量为50%)条件下油菜素内酯(BR)与氮、磷、钾(N、P、K)配合处理对羊草幼苗生长及其抗旱性的影响。结果表明:适当浓度的BR可以减轻干旱胁迫对植株的伤害,而BR与N、P、K配合(1%尿素+1%磷酸二氢钾+0.10 mg·L^-1 BR)则可进一步促进植株生长和营养物质的积累,提高羊草的抗旱性,效果更好。这种效应与其调控植株生长,提升渗透调节能力,降低膜脂的过氧化作用,增强抗氧化酶活性等生理特性密切相关。配合处理下羊草的株高、鲜重、干重和根系活力比干旱对照(CK₂)分别增加28.96%、62.46%、45.21%和19.08%,N、P、K含量分别增加20.74%、236.84%和42.31%,营养代谢相关酶硝酸还原酶(NR)、酸性磷酸酶(ACP)和苹果酸脱氢酶(MD)活性发生改变,脯氨酸、可溶性蛋白和可溶性糖分别增加57.20%、22.82%和5.94%,而丙二醛和叶片电导率分别降低37.42%和55.79%,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)分别增加158.40%、38.15%、32.97%、30.00%和28.74%。

关键词: 羊草, 油菜素内酯(BR), 干旱胁迫, 渗透调节, 酶特性

Abstract: Climate change is one of the main environmental factors with the potential to influence the productivity of Leymus chinensis. In this paper, potted plants were used to study the effects of brassinolide (BR) and nitrogen, phosphorus and potassium (N, P, K) on the growth and drought resistance of L. chinensis under normal (soil water content 80% of field capacity) and drought (soil water content 50% of field capacity) conditions. The results showed that appropriate BR concentration could alleviate plant injury under drought stress while the combination of BR and N, P, K (1% urea+1% potassium dihydrogen phosphate+0.10 mg·L^-1 BR) could promote plant growth and nutrient accumulation, further improving drought resistance in L. chinensis. This was closely related to key physiological characteristics including regulation of plant growth, enhanced osmotic adjustment, reduced membrane lipid peroxidation and enhanced antioxidant enzyme activity. Plant height, fresh weight, dry weight and root vigour increased by 28.96%, 62.46%, 45.21% and 19.08% in the BR treated plants and with N, P, K by 20.74%, 236.84% and 42.31% respectively compared with the CK. The activities of nitrate reductase (NR), acid phosphatase (ACP) and malate dehydrogenase (MD) changed with proline content; soluble protein and soluble sugar increased by 57.20%, 22.82% and 5.94% respectively. Malondialdehyde conductivity and leaf permeability were reduced by 37.42% and 55.79% respectively, while superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione reductase (GR) and ascorbate peroxidase (APX) activity increased (158.40%, 38.15%, 32.97%, 30.00% and 28.74% respectively).

Key words: Leymus chinensis, brassinolide (BR), drought stress, osmotic adjustment, enzyme characteristics

宋吉轩, 吕俊, 宗学凤, 何秀娟, 徐宇, 吴潇, 王三根. 干旱胁迫下BR与N、P、K配合对羊草生长及抗旱性的影响[J]. 草业学报, 2018, 27(11): 171-178.

SONG Ji-xuan, LÜ Jun, ZONG Xue-feng, HE Xiu-juan, XU Yu, WU Xiao, WANG San-gen. Effects of brassinolide and N, P, K fertiliser on growth of Leymus chinensis under drought stress[J]. Acta Prataculturae Sinica, 2018, 27(11): 171-178.

参考文献

[1] Liu G S, Li X X, Qi D M, et al. Evaluation and utilization of Leymus chinensis germplasm resources. Chinese Science Bulletin, 2016, 61(2): 271-281.
刘公社, 李晓霞, 齐冬梅, 等. 羊草种质资源的评价与利用. 科学通报, 2016, 61(2): 271-281.
[2] Wang Y L, Yun W L, Wang W, et al. Effect of climate warming on spatial pattern and temporal change of precipitation in typical steppe. Journal of Arid Land Resources and Environment, 2009, 23(1): 82-85.
王永利, 云文丽, 王炜,等. 气候变暖对典型草原区降水时空分布格局的影响. 干旱区资源与环境, 2009, 23(1): 82-85.
[3] He S X, Liang Z S, Wei L Z, et al. Growth and physiological characteristics of wild sour jujube seedlings from two provenances under soil water stress. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(7): 1387-1393.
贺少轩, 梁宗锁, 蔚丽珍, 等. 土壤干旱对2个种源野生酸枣幼苗生长和生理特性的影响. 西北植物学报, 2009, 29(7): 1387-1393.
[4] Todaka D K, Shinozaki, Shinozaki K Y. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Frontiers in Plant Science, 2015, 6: 1-20.
[5] Li X, Liu Z, Wang Z, et al. Pathways of Leymus chinensis individual aboveground biomass decline in natural semiarid grassland induced by overgrazing: A study at the plant functional trait scale. PloS One, 2015, 10(5): e0124443.
[6] Liu X, Chan Z.Application of potassium polyacrylate increases soil water status and improves growth of bermuda grass (Cynodon dactylon) under drought stress condition. Scientia Horticulturae, 2015, 197: 705-711.
[7] Anuradha S, Ssr R.The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress. Plant Soil Environment, 2007, 53: 465-472.
[8] Hu W H, Wu Y, Zeng J Z, et al. Chill-induced inhibition of photosynthesis was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery. Photosynthetica, 2010, 48: 537-544.
[9] Vardhini B V.Modifications of morphological and anatomical characteristics of plants by application of brassinosteroids under various abiotic stress conditions-A review. Plant Gene, 2017, 11: 70-89.
[10] Vurukonda S S K, Vardharajula S, Shrivastava M, et al. Enhancement of drought stress tolerance in crops by plant growth promoting rhizo-bacteria. Microbiological Research, 2016, 184: 13-24.
[11] Dechant B, Matthias C, Michael V, et al. Estimation of photosynthesis traits from leaf reflectance spectra: Correlation to nitrogen content as the dominant mechanism. Remote Sensing of Environment, 2017, 196: 279-292.
[12] Li Y T, Li X Y, Xiao Y, et al. Advances in study on mechanism of foliar nutrition and development of foliar fertilizer application. Scientia Agricultura Sinica, 2009, 42(1): 162-172.
李燕婷, 李秀英, 肖艳, 等. 叶面肥的营养机理及应用研究进展. 中国农业科学, 2009, 42(1): 162-172.
[13] Khalid K A, Shedeed M R.Effect of NPK and foliar nutrition on growth, yield and chemical constituents in Nigella sativa L. Journal of Materials and Environmental Science, 2015, 6: 1709-1714.
[14] Song J X, Li J H, Liu M R, et al. Effects of drought stress and BR application on osmotic adjustment and antioxidant enzymes of Leymus chinensis. Acta Prataculturae Sinica, 2015, 24(8): 93-102.
宋吉轩, 李金还, 刘美茹, 等. 油菜素内酯对干旱胁迫下羊草渗透调节及抗氧化酶的影响研究. 草业学报, 2015, 24(8): 93-102.
[15] Liu M R, Li J H, Niu J H, et al. Comparative study on gibberellin and chemical reagent on effect of germination rate and vigor of Leymus chinensis. Southwest China Journal of Agricultural Sciences, 2014, 27(6): 2687-2691.
刘美茹, 李金还, 牛建行, 等. 赤霉素与化学试剂提高羊草种子萌发率及活力的比较研究. 西南农业学报, 2014, 27(6): 2687-2691.
[16] Higa A, Mori Y, Kitamura Y.Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus. Plant Physiological, 2010, 167(11): 870-878.
[17] Zhu W Z, Cao M, Wang S G, et al. Seasonal dynamics of mobile carbon supply in Quercus aquifolioides at the upper elevational limit. PloS One, 2012, 7(3): e34213.
[18] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.
[19] Li Z G, Li J H, Du C K, et al. Simultaneous measurement of five antioxidant enzyme activities using a single extraction system. Journal of Yunnan Normal University, 2002, 22(6): 44-48.
李忠光, 李江鸿, 杜朝昆, 等. 在单一提取系统中同时测定五种植物抗氧化酶. 云南师范大学学报, 2002, 22(6): 44-48.
[20] Huang C B, Zeng F J, Lei J Q, et al. Effects of irrigation on plant growth and nitrogen use characteristics of Calligonum caput-medusae Schrenk seedlings. Acta Ecologica Sinica, 2014, 34(3): 572-580.
黄彩变, 曾凡江, 雷加强, 等. 灌溉对沙拐枣幼苗生长及氮素利用的影响. 生态学报, 2014, 34(3): 572-580.
[21] Wu D T, Zhang X X, Gong Z P, et al. Effects of phosphorus nutrition on P absorption and yields of soybean. Plant Nutrition and Fertilizer Science, 2012, 18(3): 670-677.
吴冬婷, 张晓雪, 龚振平, 等. 磷素营养对大豆磷素吸收及产量的影响. 植物营养与肥料学报, 2012, 18(3): 670-677.
[22] Mao F Y, Wang Z.Comparative study on detection methods of total potassium in crops. Modern Agricultural Science and Technology, 2014, (3): 237-238.
毛飞云, 王忠. 作物全钾检测方法比较研究. 现代农业科技, 2014, (3): 237-238.
[23] Chen W, Zhang D Y.Extraction, determination and purification of nitrate reductase in plant tissue. Plant Physiology Communications, 1980, (4): 45-49.
陈薇, 张德颐. 植物组织中硝酸还原酶的提取、测定和纯化. 植物生理学通讯, 1980, (4): 45-49.
[24] Tian J, Liao H, Wang X R, et al. Phosphorus starvation induced expression of leaf acid phosphatase isoforms in soybean. Acta Botanica Sinica, 2003, 45: 1037-1042.
[25] Sayre R T, Kennedy R A, Pringnitz D J.Photosynthetic enzyme activitives and localization in Mollugo verticillata populations in the levels of C₃ and C₄ cycle operations. Plant Physiology, 1979, 64: 293-299.
[26] Shabbir R N, Ahsraf M Y, Waraich E A, et al. Combined effects of drought stress and NPK foliar spray on growth, physiological processes and nutrient uptake in wheat. Pakistan Journal of Botany, 2015, 47: 1207-1216.
[27] Bakhshandeh E, Hemmatollah P, Khadijeh S L.Phosphate and potassium-solubilizing bacteria effect on the growth of rice. Ecological Engineering, 2017, 103: 164-169.
[28] Liu M R.Regulating effects of chemical reagent on the seed vigor and seedling growth of Leymus chinensis (Trin.). Chongqing: Southwest University, 2015.
刘美茹. 化学试剂调节对羊草种子活力及幼苗生长的影响研究. 重庆: 西南大学, 2015.
[29] Lai J S.Study on the effect of foliar spray inorganic nutrition and different plant growth regulators. Chongqing: Southwest University, 2003.
赖锦山. 叶面无机营养+不同植物生长调节剂的效应研究. 重庆: 西南大学, 2003.
[30] Ahanger M A, Agarwal R M.Salinity stress induced alterations in antioxidant metabolism and nitrogen assimilation in wheat (Triticum aestivum L.) as influenced by potassium supplementation. Plant Physiology and Biochemistry, 2017, 115: 449-460.
[31] Hofer D, Suter M, Buchmann N, et al. Nitrogen status of functionally different forage species explains resistance to severe drought and post-drought over compensation. Agriculture Ecosystems & Environment, 2017, 236: 312-322.
[32] Liang X, Liu A Q, Ma X Q, et al. The effect of phosphorus deficiency stress on activities of acid phosphatase in different clones of Chinese fir. Acta Phytoecologica Sinica, 2005, 1: 54-59.
梁霞, 刘爱琴, 马祥庆, 等. 磷胁迫对不同杉木无性系酸性磷酸酶活性的影响. 植物生态学报, 2005, 1: 54-59.
[33] Liu S, Meng J, Jiang L L, et al. Rice husk biochar impacts soil phosphorous availability, phosphatase activities and bacterial community characteristics in three different soil types. Applied Soil Ecology, 2017, 116: 12-22.
[34] Bybordi A.Influence of exogenous application of silicon and potassium on physiological responses, yield and yield components of salt-stressed wheat. Communications in Soil Science & Plant Analysis, 2014, 46: 109-122.
[35] Neshev N M.Content and uptake of nutrients with plant biomass of potatoes depending on potassium fertilization. Agriculture and Agricultural Science Procedia, 2015, 6: 63-66.
[36] Liu J H, Zhao H C, Ren Y F, et al. Change of osmotica in oat leaf under soil moisture stress. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(7): 1432-1436.
刘景辉, 赵海超, 任永峰, 等. 土壤水分胁迫对燕麦叶片渗透调节物质含量的影响. 西北植物学报, 2009, 29(7): 1432-1436.
[37] Liu Z C, Bao D E.Effect of water stress on growth and physiological indexes in Jinguang plum seedlings. Journal of Agricultural University of Hebei, 2007, 30(5): 28-31.
刘遵春, 包东娥. 水分胁迫对金光杏梅幼苗生长及其生理指标的影响. 河北农业大学学报, 2007, 30(5): 28-31.
[38] Shi L R, Liu Z H.Influences of drought stress on antioxidative activity and osmoregulation substance of Sonchus brachyotus DC. Acta Agrestia Sinica, 2010, 18(5): 673-677.
时丽冉, 刘志华. 干旱胁迫对苣荬菜抗氧化酶和渗透调节物质的影响. 草地学报, 2010, 18(5): 673-677.
[39] Ahang J X, Kirkhan M B.Drought stress induced changes in-activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiology, 1994, 35(5): 785-791.
[40] Kosar F, Akram N A, Ashraf M.Exogenously-applied 5-aminolevulinic acid modulates some key physiological characteristics and antioxidative defense system in spring wheat (Triticum aestivum L.) seedlings under water stress. South African Journal of Botany, 2015, 96: 71-77.