草业学报 ›› 2021, Vol. 30 ›› Issue (7): 157-166.DOI: 10.11686/cyxb2020249
王诗雅1(), 郑殿峰2(), 冯乃杰2(), 梁喜龙1,3, 项洪涛4, 冯胜杰1, 王新欣1, 左官强1
收稿日期:
2020-05-27
修回日期:
2020-08-10
出版日期:
2021-07-20
发布日期:
2021-06-03
通讯作者:
郑殿峰,冯乃杰
作者简介:
Corresponding author. E-mail: zdffnj@163.com基金资助:
Shi-ya WANG1(), Dian-feng ZHENG2(), Nai-jie FENG2(), Xi-long LIANG1,3, Hong-tao XIANG4, Sheng-jie FENG1, Xin-xin WANG1, Guan-qiang ZUO1
Received:
2020-05-27
Revised:
2020-08-10
Online:
2021-07-20
Published:
2021-06-03
Contact:
Dian-feng ZHENG,Nai-jie FENG
摘要:
为研究淹水胁迫下喷施烯效唑(S3307)对不同大豆品种的影响,以耐涝品种‘垦丰14’和涝渍敏感品种‘垦丰16’大豆为试验材料,进行盆栽试验,研究鼓粒期(R5)淹水胁迫对大豆叶片膜脂过氧化程度(MDA)、活性氧(ROS)和抗坏血酸-谷胱甘肽(AsA-GSH)循环系统的损伤及S3307的缓解效应。结果表明:R5期淹水胁迫显著增加了两品种大豆叶片内MDA含量,加速了ROS的积累,且相同时间垦丰16增幅大于垦丰14。叶面喷施S3307可有效提高非酶抗氧化剂含量,增加关键酶活性,降低叶片MDA含量,抑制ROS积累,减少淹水胁迫对膜系统造成的伤害,并在恢复正常水分处理后,维持较高的关键酶活性和非酶抗氧化剂含量,促进两品种大豆叶片恢复至正常状态,且垦丰14恢复能力优于垦丰16。综上,淹水胁迫对两种耐涝性不同的大豆品种叶片中AsA-GSH循环具有不同程度的影响,S3307可在一定程度上减缓淹水胁迫所造成的危害。
王诗雅, 郑殿峰, 冯乃杰, 梁喜龙, 项洪涛, 冯胜杰, 王新欣, 左官强. 鼓粒期淹水胁迫对大豆叶片AsA-GSH循环的损伤及烯效唑的缓解效应[J]. 草业学报, 2021, 30(7): 157-166.
Shi-ya WANG, Dian-feng ZHENG, Nai-jie FENG, Xi-long LIANG, Hong-tao XIANG, Sheng-jie FENG, Xin-xin WANG, Guan-qiang ZUO. Damage to the AsA-GSH cycle of soybean leaves under waterlogging stress at in seed filling period growth stages and the mitigation effect of uniconazole[J]. Acta Prataculturae Sinica, 2021, 30(7): 157-166.
品种Varieties | 处理编号Treatment code | 药剂处理Pesticide treatment | 水分处理Water treatment |
---|---|---|---|
垦丰14 Kenfeng 14 | T1 | 清水喷施 Spray water | 正常水分 Normal water |
T2 | 清水喷施 Spray water | 淹水胁迫 Waterlogging stress | |
T3 | S3307喷施 Spray S3307 | 淹水胁迫 Waterlogging stress | |
垦丰16 Kenfeng 16 | T4 | 清水喷施 Spray water | 正常水分 Normal water |
T5 | 清水喷施 Spray water | 淹水胁迫 Waterlogging stress | |
T6 | S3307喷施 Spray S3307 | 淹水胁迫 Waterlogging stress |
表1 试验设计方案
Table 1 Experiment design
品种Varieties | 处理编号Treatment code | 药剂处理Pesticide treatment | 水分处理Water treatment |
---|---|---|---|
垦丰14 Kenfeng 14 | T1 | 清水喷施 Spray water | 正常水分 Normal water |
T2 | 清水喷施 Spray water | 淹水胁迫 Waterlogging stress | |
T3 | S3307喷施 Spray S3307 | 淹水胁迫 Waterlogging stress | |
垦丰16 Kenfeng 16 | T4 | 清水喷施 Spray water | 正常水分 Normal water |
T5 | 清水喷施 Spray water | 淹水胁迫 Waterlogging stress | |
T6 | S3307喷施 Spray S3307 | 淹水胁迫 Waterlogging stress |
图1 烯效唑对R5期淹水胁迫下大豆叶片MDA含量的影响不同字母表示在0.05水平差异显著,下同。Different letters indicate significant difference at 0.05 level. The same below.
Fig.1 Effects of S3307 on MDA content in leaves of soybean under waterlogging stress at R5 stage
图2 烯效唑对R5期淹水胁迫下大豆叶片O2—·产生速率和H2O2含量的影响
Fig.2 Effects of S3307 on O2—· production rate and H2O2 content in leaves of soybean under waterlogging stress at R5 stage
图3 烯效唑对R5期淹水胁迫下大豆叶片AsA、DHA和AsA+DHA含量的影响
Fig.3 Effects of S3307 on AsA and DHA and AsA+DHA content in leaves of soybean under waterlogging stress at R5 stage
图4 烯效唑对R5期淹水胁迫下大豆叶片GSH、GSSG和GSH+GSSG含量的影响
Fig.4 Effects of S3307 on GSH and GSSG and GSH+GSSG content in leaves of soybean under waterlogging stress at R5 stage
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