草业学报 ›› 2021, Vol. 30 ›› Issue (9): 127-136.DOI: 10.11686/cyxb2020317
• 研究论文 • 上一篇
收稿日期:
2020-07-06
修回日期:
2020-09-24
出版日期:
2021-08-30
发布日期:
2021-08-30
通讯作者:
刘天增
作者简介:
Corresponding author. E-mail: liutianzeng@scau.edu.cn基金资助:
Xin-tong ZHAO(), Xiao-dong CHEN, Zi-ji LI, Ju-ming ZHANG, Tian-zeng LIU()
Received:
2020-07-06
Revised:
2020-09-24
Online:
2021-08-30
Published:
2021-08-30
Contact:
Tian-zeng LIU
摘要:
以从海滨雀稗中分离得到的内生肠杆菌为材料,采用种子萌发与盆栽试验两种方式研究植物内生细菌对狗牙根耐盐性的影响。通过对狗牙根种子和幼苗接种单一内生细菌和混合内生细菌,并测定种子发芽率、苗长、根长、生物量等生长指标与叶绿素含量、相对含水量、相对电导率和叶片中钠离子、钾离子的含量综合评价植物内生细菌对狗牙根耐盐能力的调控。结果表明,在150 mmol·L-1盐胁迫下处理14 d后,接种混合菌液B3014比接种单一菌液B30更能提升狗牙根种子的萌发率、对盐胁迫下胚根及胚芽长度的增长也较显著(P<0.05)。在成坪期,与空白对照相比,接种内生细菌可以提升200 mmol·L-1盐胁迫下狗牙根的成坪质量,增加地上、地下生物量的积累、苗长与根长生长量,并提升植株叶绿素含量与叶片相对含水量,降低细胞膜破损程度。在接种内生细菌处理中,接种混合菌液B3014可以使狗牙根在盐胁迫下受到的损伤显著低于(P<0.05)接种单一菌液B30。与此同时,接种混合菌液B3014对狗牙根叶片中钠离子含量降低程度高于接种单一菌液B30,同样,接种混合菌液B3014也能更好地积累盐胁迫下狗牙根叶片内钾离子含量,增加盐胁迫下狗牙根的耐盐能力。因此,接种混合菌液B3014对盐胁迫下狗牙根种子的萌发和成坪后的耐盐能力的提升高于接种单一菌液B30。探讨植物内生细菌对狗牙根耐盐能力的调控,为提高草坪草在盐碱环境中的应用提供了新思路。
赵欣桐, 陈晓东, 李子吉, 张巨明, 刘天增. 植物内生肠杆菌对狗牙根耐盐性的调控研究[J]. 草业学报, 2021, 30(9): 127-136.
Xin-tong ZHAO, Xiao-dong CHEN, Zi-ji LI, Ju-ming ZHANG, Tian-zeng LIU. An evaluation of the effects of the plant endophyte Enterobacter on the salt tolerance of bermudagrass[J]. Acta Prataculturae Sinica, 2021, 30(9): 127-136.
图1 盐胁迫下内生细菌对狗牙根种子萌发的影响CK:对照Control; B30:接种单一菌液Inoculation of a single bacterial solution; B3014:接种混合菌液Inoculation of mixed bacterial solution.不同小写字母表示差异显著(P<0.05) Different letters indicate significant differences at the P<0.05 level.下同The same below.
Fig.1 Seed germination of bermudagrass inoculated with endophytic bacteria under salt stress
图6 盐胁迫下内生细菌对狗牙根相对含水量和电导率的影响
Fig.6 Relative water content and electrolyte leakage of bermudagrass inoculated with endophytic bacteria under salt stress
1 | Jelte R, Timothy F. Crops for a salinized world. Science, 2008, 322: 1478-1480. |
2 | Song L Y, Lin T Y, Xu J W, et al. Effects of epibrassinolide on callus growth and regeneration of Zoysia matrella (L.) Merr. under salt stress. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1440-1446. |
宋靓苑, 林恬逸, 许静雯, 等. 盐胁迫下表油菜素内酯对沟叶结缕草愈伤组织生长和再生的影响. 核农学报, 2020, 34(7): 1440-1446. | |
3 | Egamberdieva D. Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiologiae Plantarum, 2009, 31(4): 861-864. |
4 | Wang N, Gao J, Huang W J, et al. Variations in seed germination and salicylic acid protective effect between two cultivars of Astragalus membranaceus under drought and salt stress. Pratacultural Science, 2018, 35(1): 106-114. |
王楠, 高静, 黄文静, 等. 旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应. 草业科学, 2018, 35(1): 106-114. | |
5 | Zhang R, Liu Y Y, Yu F M, et al. Effects of salt stress on seed germination of Salvia coccinea and Salvia farinacea. Molecular Plant Breeding, 2018, 16(5): 1682-1689. |
张锐, 刘玉艳, 于凤鸣, 等. 盐胁迫对红花鼠尾草和蓝花鼠尾草种子萌发的影响. 分子植物育种, 2018, 16(5): 1682-1689. | |
6 | Li H Y, Chen L. Effects of salt stress on turfgrass growth and metabolism. Pratacultural Science, 2018, 35(11): 2584-2592. |
李惠英, 陈良. 盐胁迫对草坪草萌发生长及代谢的影响.草业科学, 2018, 35(11): 2584-2592. | |
7 | Zhang X, He K N, Shi C Q, et al. Effects of salt stress on growth and physiological characteristics of Shepherida argentea seedlings. Journal of Soil and Water Conservation, 2016, 30(1): 212-217. |
张雪, 贺康宁, 史常青, 等. 盐胁迫对银水牛果幼苗生长和生理特性的影响. 水土保持学报, 2016, 30(1): 212-217. | |
8 | Li Y. Effects of NaCl stress and cold stress on growth and physiological indexes of five warm season turfgrasses. Nanjing: Nanjing Agricultural University, 2013. |
李源. NaCl胁迫和低温胁迫对五种暖季型草坪草生长及生理指标的影响. 南京: 南京农业大学, 2013. | |
9 | Guo L L, Hao L H, Jia H H, et al. Effects of NaCl stress on stomatal traits, leaf gas exchange parameters, and biomass of two tomato cultivars. Chinese Journal of Applied Ecology, 2018, 29(12): 3949-3958. |
郭丽丽, 郝立华, 贾慧慧, 等. NaCl胁迫对两种番茄气孔特征、气体交换参数和生物量的影响. 应用生态学报, 2018, 29(12): 3949-3958. | |
10 | Yan X B, Yuan J H. Effects of salt stress on plant growth. Journal of Anhui Agricultural Sciences, 2019, 47(4): 30-33. |
岩学斌, 袁金海. 盐胁迫对植物生长的影响. 安徽农业科学, 2019, 47(4): 30-33. | |
11 | Munns R, Tester M. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 2008, 59: 651-681. |
12 | Liu Y M. Physiological study and root protein expression under salinity stress in four warm season turfgrasses. Shanghai: Shanghai Jiao Tong University, 2011. |
刘一明. 四种暖季型草坪草抗盐生理及根系蛋白表达差异研究. 上海: 上海交通大学, 2011. | |
13 | Chen J B, Yan J, Zhang T T, et al. Growth responses of four warm season turfgrasses to long-term salt stress. Acta Prataculturae Sinica, 2008, 17(5): 30-36. |
陈静波, 阎君, 张婷婷, 等. 四种暖季型草坪草对长期盐胁迫的生长反应. 草业学报, 2008, 17(5): 30-36. | |
14 | Munns R. Genes and salt tolerance: Bringing them together. New Phytologist, 2005, 167(3): 645-663. |
15 | Kovda V A. Loss of productive land due to salinization. Ambio, 1983, 12(2): 91-93. |
16 | Hallmann J, Mahaffee W F, Kloepper J W, et al. Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology, 1997, 43(10): 895-914. |
17 | Gao X X, Man B Y, Chen X R, et al. Identification and determination of biological characteristic of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands. Acta Prataculturae Sinica, 2013, 22(4): 137-146. |
高晓星, 满百膺, 陈秀蓉, 等. 东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定. 草业学报, 2013, 22(4): 137-146. | |
18 | Chen X D. Endophytic microorganisms are valuable resources worthy to be explored deeply. Microbiology China, 2012, 39(2): 282. |
陈向东. 植物内生菌是有待深入开发的资源宝库. 微生物学通报, 2012, 39(2): 282. | |
19 | Complant S, Clément C, Sessitsch A. Plant growth-promoting bacteria in the rhizo-and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry, 2010, 42(5): 669-678. |
20 | Glick B R. Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS Microbiology Letters, 2005, 251(1): 1-7. |
21 | Mayak S, Tirosh T, Glick B R. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiology and Biochemistry, 2004, 42(6): 565-572. |
22 | Majeed A, Abbasi M K, Hameed S, et al. Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology, 2015, 6: 198. |
23 | Simone M, Monica G, Lisa C, et al. Phenotypic traits and establishment speed of 44 turf bermudagrass accessions. Acta Agriculturae Scandinavica Section B: Soil and Plant Science, 2014, 64(8): 722-733. |
24 | Wang H L. Study on salts resistance comparision of bermudagrass under salt stress of Na2SO4. Urumqi: Xinjiang Agricultural University, 2003. |
王红玲. Na2SO4盐胁迫下狗牙根抗盐性比较研究. 乌鲁木齐: 新疆农业大学, 2003. | |
25 | Wang Z, Wu Y Q, Mao K. Research progress of bermudagrass. Pratacultural Science, 2001, 18(5): 37-41. |
王赞, 吴彦奇, 毛凯. 狗牙根研究进展. 草业科学, 2001, 18(5): 37-41. | |
26 | Li F J, Mao Z W, Li R J, et al. Inoculation of endophytic diazotrophs on hybrid bermudagrass. Pratacultural Science, 2013, 30(12): 1953-1959. |
李凤娇, 毛中伟, 黎瑞君, 等. 杂交狗牙根内生固氮菌的接种效果. 草业科学, 2013, 30(12): 1953-1959. | |
27 | Zou Q. Plant physiology experiment guide. Beijing: China Agriculture Press, 2003. |
邹琦. 植物生理学实验指导. 北京: 中国农业出版社, 2003. | |
28 | Gao J F. Plant physiology test technology. Xi’an: World Book Publishing House, 2000. |
高俊风. 植物生理学试验技术. 西安: 世界图书出版社, 2000. | |
29 | Zhang X Z, Tan G R. Plant physiology experiment technology. Shenyang: Liaoning Science and Technology Publishing House, 1989. |
张宪政, 谭桂茹. 植物生理学实验技术. 沈阳: 辽宁科学技术出版社, 1989. | |
30 | Li H S. Principles and techniques of plant physiological biochemical experiment (textbook series for the 21st century). Beijing: Higher Education Press, 2000. |
李合生. 植物生理生化实验原理与技术(面向21世纪课程教材). 北京: 高等教育出版社, 2000. | |
31 | Wang J, Chen X, Li M, et al. Advances in research on seed germination and physiological response of different turfgrass seedlings to salt stress. Xiandai Nongcun Keji, 2020(5): 64-66. |
王君, 陈新, 李末, 等. 不同草坪草种子萌发及幼苗对盐胁迫的生理响应研究进展. 现代农村科技, 2020(5): 64-66. | |
32 | Fu L, Li X, Gao L, et al. Endophytic bacteria can mitigate salinity stress on seed germination and physiology in hybrid Pennisetum. Pratacultural Science, 2017, 34(10): 2099-2108. |
傅蕾, 李霞, 高璐, 等. 盐胁迫下泛菌属内生细菌对杂交狼尾草发芽及生理的影响. 草业科学, 2017, 34(10): 2099-2108. | |
33 | Mauricio S, Claudia M R, Martín A P, et al. Plant growth promoting properties of a strain of Enterobacter ludwigii isolated from Lolium perenne rhizosphere. Soil Biology and Biochemistry, 2007, 41(9): 1768-1774. |
34 | Wang N, Wang W W, Zhang J, et al. Effect of salt stress on the germination and growth of wheat seeds soaked with hydrogen-oxidizing bacteria WMQ7. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(10): 2065-2070. |
王楠, 王卫卫, 张洁, 等. 盐胁迫对氢氧化细菌WMQ7浸种小麦萌发和生长的影响. 西北植物学报, 2011, 31(10): 2065-2070. | |
35 | Blumwald E. Engineering salt tolerance in plants. Biotechnology and Genetic Engineering Reviews, 2003, 20(1): 261-276. |
36 | Zhang Z, Liu M, Liang Y, et al. The effects of salt stress on the growth of three cold-season turfgrass. Ecology and Environmental Sciences, 2009, 18(5): 1877-1880. |
张志, 刘敏, 梁艳, 等. 盐胁迫对3种冷季型草坪草生长的影响. 生态环境学报, 2009, 18(5): 1877-1880. | |
37 | Zheng Y Y, Yue H T, Shi Z Q, et al. Physiochemical characters and ability to promote cotton germination of bacteria strains Rs-5 and Rs-198 under salt stress. Scientia Agricultura Sinica, 2008, 41(5): 1326-1332. |
郑元元, 岳海涛, 石在强, 等. 盐胁迫下解盐促生细菌Rs-5和Rs-198促进棉花种子发芽的机理探讨. 中国农业科学, 2008, 41(5): 1326-1332. | |
38 | Sun J. Signaling network in the perception of salt stress and ionic homeostasis regulation in Populus euphratica. Beijing: Beijing Forestry University, 2011. |
孙健. 胡杨响应盐胁迫与离子平衡调控信号网络研究. 北京: 北京林业大学, 2011. | |
39 | Duan H R, Zhou X H, Hu J, et al. Advances in understanding molecular mechanisms of K+ uptake and transport in higher plants. Acta Prataculturae Sinica, 2019, 28(9): 174-191. |
段慧荣, 周学辉, 胡静, 等. 高等植物K+吸收及转运的分子机制研究进展. 草业学报, 2019, 28(9): 174-191. | |
40 | Tester M, Davenport R. Na+ tolerance and Na+ transport in higher plants. Annals of Botany, 2003, 91(5): 503-527. |
41 | Shabala S, Cuin T A, Pottosin I. Polyamines prevent NaCl-induced K+ efflux from pea mesophyll by blocking non-selective cation channels. FEBS Letters, 2007, 581(10): 1993-1999. |
42 | Li J. Physiological responses of Puccinellia tenuiflora to beneficial soil bacteria and various K+ and Na+ conditions. Lanzhou: Lanzhou University, 2015. |
李静. 小花碱茅响应土壤有益细菌和不同K+、Na+生境的生理研究. 兰州: 兰州大学, 2015. | |
43 | Xu F F, Yuan L M, Shao Y F, et al. Effect of Enterobacter sp. FYP1101 on wheat seedling growth under salt stress. Microbiology China, 2018, 45(1): 102-110. |
许芳芳, 袁立敏, 邵玉芳, 等. 肠杆菌FYP1101对盐胁迫下小麦幼苗的促生效应. 微生物学通报, 2018, 45(1): 102-110. | |
44 | Gustavo S, Gabriel M H, Del C O, et al. Plant growth-promoting bacteria endophyte. Microbiological Research, 2016, 183(5): 92-99. |
45 | Diskit D, Phuntsog D, Stanzin A, et al. Stress tolerance and plant growth promotion potential of Enterobacter ludwigii PS1 isolated from Seabuckthorn rhizosphere. Biocatalysis and Agricultural Biotechnology, 2018, 14(1): 438-443. |
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