草业学报 ›› 2024, Vol. 33 ›› Issue (2): 164-184.DOI: 10.11686/cyxb2023105
• 研究论文 • 上一篇
罗颖(), 李聪, 王沛, 田莉华, 汪辉, 周青平, 雷映霞()
收稿日期:
2023-04-04
修回日期:
2023-05-24
出版日期:
2024-02-20
发布日期:
2023-12-12
通讯作者:
雷映霞
作者简介:
E-mail: leiyingxia@hotmail.com基金资助:
Ying LUO(), Cong LI, Pei WANG, Li-hua TIAN, Hui WANG, Qing-ping ZHOU, Ying-xia LEI()
Received:
2023-04-04
Revised:
2023-05-24
Online:
2024-02-20
Published:
2023-12-12
Contact:
Ying-xia LEI
摘要:
为探讨在低氮胁迫下皮燕麦早期的形态、生理响应以及筛选出耐低氮皮燕麦品种,本研究以18种不同皮燕麦品种为材料,采用水培法,设置全氮(4 mmol·L-1,CK)和1/10 N(0.4 mmol·L-1,LN)两个氮浓度水平,在处理21 d后,对低氮胁迫下皮燕麦的7项形态指标和14项生理指标进行测定和分析;通过相关性分析、主成分降维分析提取影响因子,结合隶属函数分析和聚类分析进行综合评价。结果表明:1)低氮胁迫下不同皮燕麦品种早期的株高、地上部生物量、硝态氮含量、硝酸还原酶活性、谷氨酰胺合成酶活性、谷氨酸脱氢酶活性、可溶性糖含量与可溶性蛋白含量均呈下降趋势;谷氨酸合成酶活性、丙二醛含量、活性氧含量均呈上升趋势;根长、地下部生物量、根冠比变化趋势不尽相同;2)21项生理指标中有12对指标呈极显著相关性,根冠比(RSR)与地下部干重(RDW)呈极显著正相关关系,谷氨酰胺合成酶(GS)与可溶性蛋白(SP)呈极显著负相关;3)低氮胁迫下皮燕麦的生物量、氮代谢因子、光合因子、抗氧化因子、渗透调节因子可作为评价耐低氮性的重要指标;4)18份皮燕麦可划分为3大类,青海甜燕麦和青海444这两份品种耐低氮能力较强,为耐低氮型品种,甜燕2号和青燕2号这两份皮燕麦品种的耐低氮能力较弱,为氮敏感型品种,其余材料耐低氮性居中。
罗颖, 李聪, 王沛, 田莉华, 汪辉, 周青平, 雷映霞. 低氮胁迫下不同皮燕麦品种早期的响应研究及耐低氮性综合评价[J]. 草业学报, 2024, 33(2): 164-184.
Ying LUO, Cong LI, Pei WANG, Li-hua TIAN, Hui WANG, Qing-ping ZHOU, Ying-xia LEI. Responses of different oat cultivars to low-nitrogen stress[J]. Acta Prataculturae Sinica, 2024, 33(2): 164-184.
序号 Number | 品种 Cultivar | 品种来源 Cultivar source | 序号 Number | 品种 Cultivar | 品种来源 Cultivar source |
---|---|---|---|---|---|
1 | 蒙燕1号 Mengyan No.1 | 内蒙古农牧业科学院Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences | 10 | 太阳神Titan | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
2 | 青引1号 Qingyin No.1 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 11 | 青海444 Qinghai 444 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
3 | 枪手Shooter | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 12 | 美达Monida | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
4 | 燕王Forage plus | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 13 | 魅力Charisma | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
5 | 青燕2号 Qingyan No.2 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 14 | 领袖Souris | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
6 | 边锋Blade | 郑州华丰草业科技有限公司Zhengzhou Huafeng Grass Industry Technology Co., Ltd. | 15 | 青海甜燕麦 Qinghai sweet oat | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
7 | 骏马Cayuse | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 16 | 牧王Heymaker | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
8 | 莫妮卡Monica | 北京百斯特草业有限公司Beijing Best Grass Industry Co., Ltd. | 17 | 甜燕2号 Haywire | 北京佰青源畜牧业科技发展有限公司Beijing Baiqingyuan Animal Husbandry Science and Technology Development Co., Ltd. |
9 | 甜燕70 Sweet oat 70 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 18 | 青燕1号 Qingyan No.1 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
表1 供试燕麦品种来源
Table 1 Cultivar source of oats
序号 Number | 品种 Cultivar | 品种来源 Cultivar source | 序号 Number | 品种 Cultivar | 品种来源 Cultivar source |
---|---|---|---|---|---|
1 | 蒙燕1号 Mengyan No.1 | 内蒙古农牧业科学院Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences | 10 | 太阳神Titan | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
2 | 青引1号 Qingyin No.1 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 11 | 青海444 Qinghai 444 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
3 | 枪手Shooter | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 12 | 美达Monida | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
4 | 燕王Forage plus | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 13 | 魅力Charisma | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
5 | 青燕2号 Qingyan No.2 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 14 | 领袖Souris | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
6 | 边锋Blade | 郑州华丰草业科技有限公司Zhengzhou Huafeng Grass Industry Technology Co., Ltd. | 15 | 青海甜燕麦 Qinghai sweet oat | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
7 | 骏马Cayuse | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. | 16 | 牧王Heymaker | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd. |
8 | 莫妮卡Monica | 北京百斯特草业有限公司Beijing Best Grass Industry Co., Ltd. | 17 | 甜燕2号 Haywire | 北京佰青源畜牧业科技发展有限公司Beijing Baiqingyuan Animal Husbandry Science and Technology Development Co., Ltd. |
9 | 甜燕70 Sweet oat 70 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine | 18 | 青燕1号 Qingyan No.1 | 青海省畜牧兽医科学院Qinghai Academy of Animal Science and Veterinary Medicine |
品种 Cultivar | 处理 Treatment | 株高 Plant height (cm) | 变化趋势 Changing trends (%) | 根长 Root length (cm) | 变化趋势 Changing trends (%) | 根冠比 Root-shoot ratio | 变化趋势 Changing trends (%) | 地上部鲜重 Shoot fresh weight (g) | 变化趋势 Changing trends (%) | 地下部鲜重 Root fresh weight (g) | 变化趋势 Changing trends (%) | 地上部干重 Shoot dry weight (g) | 变化趋势 Changing trends(%) | 地下部干重 Root dry weight (g) | 变化趋势 Changing trends (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | CK | 67.57±4.37a | -2.58 | 27.77±10.73a | -28.23 | 0.10±0.04b | 20.00 | 6.89±2.04a | -30.62 | 0.72±0.23a | -18.06 | 0.60±0.16a | -20.00 | 0.06±0.02a | 0.00 |
LN | 65.83±2.75a | 19.93±3.27b | 0.12±0.02a | 4.78±1.02b | 0.59±0.30a | 0.48±0.12b | 0.06±0.02a | ||||||||
2 | CK | 87.77±0.75a | -12.30 | 21.27±3.75a | -43.58 | 0.09±0.03a | -22.22 | 9.43±2.58a | -46.98 | 1.17±1.10a | -88.89 | 0.98±0.29a | -36.73 | 0.09±0.05a | -55.56 |
LN | 76.97±17.45b | 12.00±3.36b | 0.07±0.01b | 5.00±2.74b | 0.13±0.02b | 0.62±0.37b | 0.04±0.02b | ||||||||
3 | CK | 67.67±3.71a | -21.19 | 22.90±9.47a | -17.34 | 0.22±0.11a | -36.36 | 3.71±0.75a | -24.26 | 0.49±0.02a | -28.57 | 0.28±0.06a | 0.00 | 0.06±0.01a | -33.33 |
LN | 53.33±30.33b | 18.93±8.11b | 0.14±0.02a | 2.81±0.67b | 0.35±0.21b | 0.28±0.09a | 0.04±0.01b | ||||||||
4 | CK | 76.87±3.75a | -8.38 | 16.03±2.24a | -10.98 | 0.10±0.02a | -20.00 | 6.15±0.34a | -39.51 | 0.64±0.18a | -43.94 | 0.71±0.06a | -42.25 | 0.07±0.02a | -57.14 |
LN | 70.43±3.35b | 14.27±3.51a | 0.08±0.06b | 3.72±1.18b | 0.33±0.12b | 0.41±0.14b | 0.03±0.01b | ||||||||
5 | CK | 86.50±9.55a | -2.66 | 31.40±9.64a | -54.78 | 0.09±0.03a | -33.33 | 11.51±2.04a | -51.22 | 1.02±0.45a | -67.65 | 1.03±0.21a | -44.66 | 0.09±0.03a | -66.67 |
LN | 84.20±14.12a | 14.20±5.09b | 0.06±0.02b | 5.61±2.47b | 0.33±0.20b | 0.57±0.29b | 0.03±0.01b | ||||||||
6 | CK | 73.83±3.20a | -6.95 | 22.90±4.97b | 25.20 | 0.10±0.02b | 80.00 | 4.71±1.21a | -20.38 | 0.51±0.14b | 60.78 | 0.41±0.04a | -17.07 | 0.04±0.01b | 50.00 |
LN | 68.70±3.46b | 28.67±15.47a | 0.18±0.06a | 3.75±0.88b | 0.82±0.23a | 0.34±0.11a | 0.06±0.02a | ||||||||
7 | CK | 73.00±2.42a | -8.95 | 22.70±4.61a | -3.83 | 0.14±0.05b | 57.14 | 7.73±0.19a | -69.73 | 0.99±0.13a | -38.38 | 0.54±0.14a | -66.67 | 0.07±0.01a | -42.86 |
LN | 66.47±4.13b | 21.83±1.91a | 0.22±0.00a | 2.34±0.44b | 0.61±0.12b | 0.18±0.05b | 0.04±0.01b | ||||||||
8 | CK | 65.73±4.09a | -6.28 | 16.80±5.09b | 20.65 | 0.10±0.01b | 40.00 | 4.31±1.28a | -40.60 | 0.51±0.21a | -49.02 | 0.43±0.17a | -34.88 | 0.04±0.01a | 0.00 |
LN | 61.60±2.82b | 20.27±3.66a | 0.14±0.04a | 2.56±0.20b | 0.26±0.08b | 0.28±0.06b | 0.04±0.01a | ||||||||
9 | CK | 74.40±10.67a | -9.85 | 20.57±8.06b | 40.35 | 0.08±0.02a | 0.00 | 4.47±1.75a | -26.17 | 0.39±0.24a | -20.51 | 0.33±0.14a | -3.03 | 0.03±0.02a | -33.33 |
LN | 67.07±6.77b | 28.87±9.63a | 0.08±0.01a | 3.30±1.37b | 0.31±0.16a | 0.32±0.16a | 0.02±0.01b | ||||||||
10 | CK | 62.53±4.24a | -0.42 | 17.40±0.95a | -25.69 | 0.10±0.05b | 40.00 | 3.71±0.49a | -53.10 | 0.68±0.12a | -48.53 | 0.30±0.09a | -53.33 | 0.03±0.01a | -33.33 |
LN | 62.27±2.99a | 12.93±5.37b | 0.14±0.04a | 1.74±0.58b | 0.35±0.13b | 0.14±0.05b | 0.02±0.01b | ||||||||
11 | CK | 84.9±5.04a | -10.92 | 15.93±3.77b | 43.94 | 0.04±0.01b | 150.00 | 8.10±1.22a | -18.52 | 0.44±0.14b | 111.36 | 1.02±0.15a | -27.45 | 0.04±0.01b | 100.00 |
LN | 75.63±4.97b | 22.93±10.23a | 0.10±0.03a | 6.60±1.64a | 0.93±0.43a | 0.74±0.14b | 0.08±0.04a | ||||||||
12 | CK | 66.87±5.28a | -5.08 | 23.13±7.42a | -13.53 | 0.09±0.01a | 11.11 | 4.74±0.89a | -13.71 | 0.56±0.08a | -14.29 | 0.56±0.18a | -21.43 | 0.05±0.01a | -20.00 |
LN | 63.47±1.97a | 20.00±1.82a | 0.10±0.03a | 4.09±0.67a | 0.48±0.04a | 0.44±0.07b | 0.04±0.01a | ||||||||
13 | CK | 70.90±4.89a | -5.12 | 32.27±21.84a | -12.40 | 0.10±0.07b | 50.00 | 5.69±0.97a | -8.61 | 0.93±0.77a | 18.28 | 0.71±0.14a | -25.35 | 0.06±0.03b | 33.33 |
LN | 67.27±4.38a | 28.27±14.88a | 0.15±0.01a | 5.20±2.11a | 1.10±0.54a | 0.53±0.20b | 0.08±0.03a | ||||||||
14 | CK | 71.30±0.78a | -20.72 | 19.33±6.77a | -0.67 | 0.07±0.01b | 85.71 | 5.49±0.60a | -4.19 | 0.50±0.24b | 64.00 | 0.60±0.07a | -13.33 | 0.04±0.01b | 75.00 |
LN | 56.53±39.09b | 19.20±4.99a | 0.13±0.02a | 5.26±0.59a | 0.82±0.26a | 0.52±0.10a | 0.07±0.02a | ||||||||
15 | CK | 83.40±0.80a | -5.31 | 21.17±4.99b | 42.32 | 0.06±0.00b | 83.33 | 7.20±0.68a | -12.22 | 0.51±0.08b | 33.33 | 0.73±0.10a | -19.18 | 0.04±0.01b | 75.00 |
LN | 78.97±4.16a | 30.13±14.12a | 0.11±0.07a | 6.32±0.83a | 0.68±0.33a | 0.59±0.09b | 0.07±0.01a | ||||||||
16 | CK | 75.67±0.45a | -3.09 | 21.13±6.33a | -0.62 | 0.09±0.03b | 77.78 | 5.69±0.72a | -9.49 | 0.93±0.59a | 29.03 | 0.50±0.08a | -4.00 | 0.05±0.03b | 60.00 |
LN | 73.33±8.90a | 21.00±14.25a | 0.16±0.02a | 5.15±2.67a | 1.20±1.07a | 0.48±0.28a | 0.08±0.05a | ||||||||
17 | CK | 80.40±5.84a | -27.36 | 29.27±13.01a | -55.93 | 0.11±0.07a | 0.00 | 5.76±0.95a | -45.49 | 0.98±0.72a | -67.35 | 0.59±0.11a | -50.85 | 0.06±0.03a | -50.00 |
LN | 58.40±8.25b | 12.90±3.22b | 0.11±0.04a | 3.14±1.34b | 0.32±0.30b | 0.29±0.12b | 0.03±0.02b | ||||||||
18 | CK | 80.80±3.47a | -12.25 | 25.97±16.14a | -21.45 | 0.09±0.05a | -11.11 | 5.18±0.54a | -6.56 | 0.52±0.21a | -1.92 | 0.57±0.11a | -1.75 | 0.05±0.02a | -20.00 |
LN | 70.90±1.40b | 20.40±0.75b | 0.08±0.03a | 4.84±0.74a | 0.51±0.16a | 0.56±0.09a | 0.04±0.01b |
表2 低氮胁迫下各皮燕麦品种的生长变化
Table 2 Effects of low nitrogen stress on the growth of different oat cultivars
品种 Cultivar | 处理 Treatment | 株高 Plant height (cm) | 变化趋势 Changing trends (%) | 根长 Root length (cm) | 变化趋势 Changing trends (%) | 根冠比 Root-shoot ratio | 变化趋势 Changing trends (%) | 地上部鲜重 Shoot fresh weight (g) | 变化趋势 Changing trends (%) | 地下部鲜重 Root fresh weight (g) | 变化趋势 Changing trends (%) | 地上部干重 Shoot dry weight (g) | 变化趋势 Changing trends(%) | 地下部干重 Root dry weight (g) | 变化趋势 Changing trends (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | CK | 67.57±4.37a | -2.58 | 27.77±10.73a | -28.23 | 0.10±0.04b | 20.00 | 6.89±2.04a | -30.62 | 0.72±0.23a | -18.06 | 0.60±0.16a | -20.00 | 0.06±0.02a | 0.00 |
LN | 65.83±2.75a | 19.93±3.27b | 0.12±0.02a | 4.78±1.02b | 0.59±0.30a | 0.48±0.12b | 0.06±0.02a | ||||||||
2 | CK | 87.77±0.75a | -12.30 | 21.27±3.75a | -43.58 | 0.09±0.03a | -22.22 | 9.43±2.58a | -46.98 | 1.17±1.10a | -88.89 | 0.98±0.29a | -36.73 | 0.09±0.05a | -55.56 |
LN | 76.97±17.45b | 12.00±3.36b | 0.07±0.01b | 5.00±2.74b | 0.13±0.02b | 0.62±0.37b | 0.04±0.02b | ||||||||
3 | CK | 67.67±3.71a | -21.19 | 22.90±9.47a | -17.34 | 0.22±0.11a | -36.36 | 3.71±0.75a | -24.26 | 0.49±0.02a | -28.57 | 0.28±0.06a | 0.00 | 0.06±0.01a | -33.33 |
LN | 53.33±30.33b | 18.93±8.11b | 0.14±0.02a | 2.81±0.67b | 0.35±0.21b | 0.28±0.09a | 0.04±0.01b | ||||||||
4 | CK | 76.87±3.75a | -8.38 | 16.03±2.24a | -10.98 | 0.10±0.02a | -20.00 | 6.15±0.34a | -39.51 | 0.64±0.18a | -43.94 | 0.71±0.06a | -42.25 | 0.07±0.02a | -57.14 |
LN | 70.43±3.35b | 14.27±3.51a | 0.08±0.06b | 3.72±1.18b | 0.33±0.12b | 0.41±0.14b | 0.03±0.01b | ||||||||
5 | CK | 86.50±9.55a | -2.66 | 31.40±9.64a | -54.78 | 0.09±0.03a | -33.33 | 11.51±2.04a | -51.22 | 1.02±0.45a | -67.65 | 1.03±0.21a | -44.66 | 0.09±0.03a | -66.67 |
LN | 84.20±14.12a | 14.20±5.09b | 0.06±0.02b | 5.61±2.47b | 0.33±0.20b | 0.57±0.29b | 0.03±0.01b | ||||||||
6 | CK | 73.83±3.20a | -6.95 | 22.90±4.97b | 25.20 | 0.10±0.02b | 80.00 | 4.71±1.21a | -20.38 | 0.51±0.14b | 60.78 | 0.41±0.04a | -17.07 | 0.04±0.01b | 50.00 |
LN | 68.70±3.46b | 28.67±15.47a | 0.18±0.06a | 3.75±0.88b | 0.82±0.23a | 0.34±0.11a | 0.06±0.02a | ||||||||
7 | CK | 73.00±2.42a | -8.95 | 22.70±4.61a | -3.83 | 0.14±0.05b | 57.14 | 7.73±0.19a | -69.73 | 0.99±0.13a | -38.38 | 0.54±0.14a | -66.67 | 0.07±0.01a | -42.86 |
LN | 66.47±4.13b | 21.83±1.91a | 0.22±0.00a | 2.34±0.44b | 0.61±0.12b | 0.18±0.05b | 0.04±0.01b | ||||||||
8 | CK | 65.73±4.09a | -6.28 | 16.80±5.09b | 20.65 | 0.10±0.01b | 40.00 | 4.31±1.28a | -40.60 | 0.51±0.21a | -49.02 | 0.43±0.17a | -34.88 | 0.04±0.01a | 0.00 |
LN | 61.60±2.82b | 20.27±3.66a | 0.14±0.04a | 2.56±0.20b | 0.26±0.08b | 0.28±0.06b | 0.04±0.01a | ||||||||
9 | CK | 74.40±10.67a | -9.85 | 20.57±8.06b | 40.35 | 0.08±0.02a | 0.00 | 4.47±1.75a | -26.17 | 0.39±0.24a | -20.51 | 0.33±0.14a | -3.03 | 0.03±0.02a | -33.33 |
LN | 67.07±6.77b | 28.87±9.63a | 0.08±0.01a | 3.30±1.37b | 0.31±0.16a | 0.32±0.16a | 0.02±0.01b | ||||||||
10 | CK | 62.53±4.24a | -0.42 | 17.40±0.95a | -25.69 | 0.10±0.05b | 40.00 | 3.71±0.49a | -53.10 | 0.68±0.12a | -48.53 | 0.30±0.09a | -53.33 | 0.03±0.01a | -33.33 |
LN | 62.27±2.99a | 12.93±5.37b | 0.14±0.04a | 1.74±0.58b | 0.35±0.13b | 0.14±0.05b | 0.02±0.01b | ||||||||
11 | CK | 84.9±5.04a | -10.92 | 15.93±3.77b | 43.94 | 0.04±0.01b | 150.00 | 8.10±1.22a | -18.52 | 0.44±0.14b | 111.36 | 1.02±0.15a | -27.45 | 0.04±0.01b | 100.00 |
LN | 75.63±4.97b | 22.93±10.23a | 0.10±0.03a | 6.60±1.64a | 0.93±0.43a | 0.74±0.14b | 0.08±0.04a | ||||||||
12 | CK | 66.87±5.28a | -5.08 | 23.13±7.42a | -13.53 | 0.09±0.01a | 11.11 | 4.74±0.89a | -13.71 | 0.56±0.08a | -14.29 | 0.56±0.18a | -21.43 | 0.05±0.01a | -20.00 |
LN | 63.47±1.97a | 20.00±1.82a | 0.10±0.03a | 4.09±0.67a | 0.48±0.04a | 0.44±0.07b | 0.04±0.01a | ||||||||
13 | CK | 70.90±4.89a | -5.12 | 32.27±21.84a | -12.40 | 0.10±0.07b | 50.00 | 5.69±0.97a | -8.61 | 0.93±0.77a | 18.28 | 0.71±0.14a | -25.35 | 0.06±0.03b | 33.33 |
LN | 67.27±4.38a | 28.27±14.88a | 0.15±0.01a | 5.20±2.11a | 1.10±0.54a | 0.53±0.20b | 0.08±0.03a | ||||||||
14 | CK | 71.30±0.78a | -20.72 | 19.33±6.77a | -0.67 | 0.07±0.01b | 85.71 | 5.49±0.60a | -4.19 | 0.50±0.24b | 64.00 | 0.60±0.07a | -13.33 | 0.04±0.01b | 75.00 |
LN | 56.53±39.09b | 19.20±4.99a | 0.13±0.02a | 5.26±0.59a | 0.82±0.26a | 0.52±0.10a | 0.07±0.02a | ||||||||
15 | CK | 83.40±0.80a | -5.31 | 21.17±4.99b | 42.32 | 0.06±0.00b | 83.33 | 7.20±0.68a | -12.22 | 0.51±0.08b | 33.33 | 0.73±0.10a | -19.18 | 0.04±0.01b | 75.00 |
LN | 78.97±4.16a | 30.13±14.12a | 0.11±0.07a | 6.32±0.83a | 0.68±0.33a | 0.59±0.09b | 0.07±0.01a | ||||||||
16 | CK | 75.67±0.45a | -3.09 | 21.13±6.33a | -0.62 | 0.09±0.03b | 77.78 | 5.69±0.72a | -9.49 | 0.93±0.59a | 29.03 | 0.50±0.08a | -4.00 | 0.05±0.03b | 60.00 |
LN | 73.33±8.90a | 21.00±14.25a | 0.16±0.02a | 5.15±2.67a | 1.20±1.07a | 0.48±0.28a | 0.08±0.05a | ||||||||
17 | CK | 80.40±5.84a | -27.36 | 29.27±13.01a | -55.93 | 0.11±0.07a | 0.00 | 5.76±0.95a | -45.49 | 0.98±0.72a | -67.35 | 0.59±0.11a | -50.85 | 0.06±0.03a | -50.00 |
LN | 58.40±8.25b | 12.90±3.22b | 0.11±0.04a | 3.14±1.34b | 0.32±0.30b | 0.29±0.12b | 0.03±0.02b | ||||||||
18 | CK | 80.80±3.47a | -12.25 | 25.97±16.14a | -21.45 | 0.09±0.05a | -11.11 | 5.18±0.54a | -6.56 | 0.52±0.21a | -1.92 | 0.57±0.11a | -1.75 | 0.05±0.02a | -20.00 |
LN | 70.90±1.40b | 20.40±0.75b | 0.08±0.03a | 4.84±0.74a | 0.51±0.16a | 0.56±0.09a | 0.04±0.01b |
品种 Cultivar | 处理 Treatment | 净光合速率 Photosynthesis rate (μmol·m-2·s-1) | 变化趋势 Changing trends (%) | 蒸腾速率 Transpiration rate (mmol·m-2·s-1) | 变化趋势 Changing trends (%) | 胞间CO2浓度 Internal CO2 concentration (μmol·m-1) | 变化趋势 Changing trends (%) | 气孔导度 Stomatal conductance (mol·m-2·s-1) | 变化趋势 Changing trends (%) |
---|---|---|---|---|---|---|---|---|---|
1 | CK | 8.64±2.84a | -59.14 | 1.93±0.40a | -34.20 | 340.60±4.34a | -8.70 | 0.17±0.03a | -35.29 |
LN | 3.53±0.19b | 1.27±0.10b | 310.97±13.65a | 0.11±0.01b | |||||
2 | CK | 10.00±0.14a | -45.40 | 2.97±0.04a | -37.37 | 338.57±5.98a | -10.36 | 0.18±0.00a | -11.11 |
LN | 5.46±1.32b | 1.86±0.49b | 303.50±2.02b | 0.16±0.04a | |||||
3 | CK | 9.52±0.22a | -37.18 | 3.34±0.05a | -62.57 | 300.63±2.04a | -0.50 | 0.17±0.00a | -41.18 |
LN | 5.98±2.81b | 1.25±0.53b | 299.13±6.32a | 0.10±0.04b | |||||
4 | CK | 12.39±1.95a | -27.85 | 4.38±0.18a | -30.82 | 328.99±1.38a | -4.72 | 0.26±0.03a | -7.69 |
LN | 8.94±0.39b | 3.03±0.32b | 313.45±4.10a | 0.24±0.01a | |||||
5 | CK | 9.39±1.14a | -44.20 | 3.34±1.06a | -49.10 | 336.42±32.39a | -0.38 | 0.32±0.11a | -53.13 |
LN | 5.24±0.04b | 1.70±0.10b | 335.13±2.83a | 0.15±0.01b | |||||
6 | CK | 7.05±1.78a | -23.97 | 2.92±0.26a | -62.67 | 349.66±7.19a | -15.91 | 0.26±0.03a | -65.38 |
LN | 5.36±0.90b | 1.09±0.06b | 294.04±23.24b | 0.09±0.01b | |||||
7 | CK | 10.69±3.21a | -56.59 | 3.98±1.26a | -32.91 | 370.27±12.45a | -14.01 | 0.39±0.15a | -41.03 |
LN | 4.64±0.25b | 2.67±0.62b | 318.39±4.38b | 0.23±0.06b | |||||
8 | CK | 7.72±2.15a | -52.85 | 3.71±0.67a | -71.43 | 355.40±4.38a | -10.01 | 0.34±0.08a | -76.47 |
LN | 3.64±0.73b | 1.06±0.17b | 319.81±26.79b | 0.08±0.01b | |||||
9 | CK | 4.77±0.67a | -13.00 | 2.06±0.12a | -37.86 | 354.25±12.60a | -9.32 | 0.18±0.01a | -38.89 |
LN | 4.15±1.31a | 1.28±0.03b | 321.24±9.35a | 0.11±0.00b | |||||
10 | CK | 5.76±0.98a | -12.15 | 2.31±2.04a | -37.23 | 324.94±26.20a | -0.57 | 0.21±0.21a | -42.86 |
LN | 5.06±2.03a | 1.45±0.09b | 323.09±41.44a | 0.12±0.01b | |||||
11 | CK | 4.24±1.52a | -12.97 | 1.37±0.24a | -29.93 | 332.04±12.13a | -5.75 | 0.11±0.02a | -27.27 |
LN | 3.69±0.47a | 0.96±0.08b | 312.95±7.67a | 0.08±0.01b | |||||
12 | CK | 8.61±0.24a | -31.71 | 1.75±0.12a | -17.14 | 309.24±2.86a | -3.57 | 0.15±0.01a | -26.67 |
LN | 5.88±0.95b | 1.45±0.27a | 298.21±8.52a | 0.11±0.02b | |||||
13 | CK | 9.94±0.03a | -18.61 | 2.72±0.11a | -31.25 | 325.69±3.66a | -6.23 | 0.24±0.01a | -37.50 |
LN | 8.09±2.13a | 1.87±0.44b | 305.40±1.54a | 0.15±0.04b | |||||
14 | CK | 7.09±2.93a | -44.57 | 1.51±0.75a | -41.06 | 297.28±7.27a | -0.33 | 0.13±0.07a | -46.15 |
LN | 3.93±1.20b | 0.89±0.31b | 296.31±14.26a | 0.07±0.02b | |||||
15 | CK | 8.78±0.13a | -10.82 | 4.76±0.05a | -57.77 | 339.46±1.09a | -6.70 | 0.28±0.00a | -35.71 |
LN | 7.83±1.09a | 2.01±0.49b | 316.72±26.10a | 0.18±0.05b | |||||
16 | CK | 9.47±0.24a | -64.52 | 2.68±0.08a | -77.61 | 298.54±2.36a | -10.75 | 0.16±0.00a | -75.00 |
LN | 3.36±0.73b | 0.60±0.13b | 266.45±4.66b | 0.04±0.01b | |||||
17 | CK | 6.78±0.52a | -62.24 | 1.54±0.25a | -62.34 | 303.70±14.75a | -2.41 | 0.12±0.01a | -66.67 |
LN | 2.56±0.41b | 0.58±0.05b | 296.39±9.02a | 0.04±0.00b | |||||
18 | CK | 6.03±0.78a | -3.32 | 1.30±0.06a | -36.92 | 293.10±6.50a | -18.27 | 0.10±0.01a | -40.00 |
LN | 5.83±0.51a | 0.82±0.06b | 239.54±3.85b | 0.06±0.00b |
表3 低氮胁迫下各皮燕麦品种光合作用的变化
Table 3 Effects of low nitrogen stress on photosynthesis of different oat cultivars
品种 Cultivar | 处理 Treatment | 净光合速率 Photosynthesis rate (μmol·m-2·s-1) | 变化趋势 Changing trends (%) | 蒸腾速率 Transpiration rate (mmol·m-2·s-1) | 变化趋势 Changing trends (%) | 胞间CO2浓度 Internal CO2 concentration (μmol·m-1) | 变化趋势 Changing trends (%) | 气孔导度 Stomatal conductance (mol·m-2·s-1) | 变化趋势 Changing trends (%) |
---|---|---|---|---|---|---|---|---|---|
1 | CK | 8.64±2.84a | -59.14 | 1.93±0.40a | -34.20 | 340.60±4.34a | -8.70 | 0.17±0.03a | -35.29 |
LN | 3.53±0.19b | 1.27±0.10b | 310.97±13.65a | 0.11±0.01b | |||||
2 | CK | 10.00±0.14a | -45.40 | 2.97±0.04a | -37.37 | 338.57±5.98a | -10.36 | 0.18±0.00a | -11.11 |
LN | 5.46±1.32b | 1.86±0.49b | 303.50±2.02b | 0.16±0.04a | |||||
3 | CK | 9.52±0.22a | -37.18 | 3.34±0.05a | -62.57 | 300.63±2.04a | -0.50 | 0.17±0.00a | -41.18 |
LN | 5.98±2.81b | 1.25±0.53b | 299.13±6.32a | 0.10±0.04b | |||||
4 | CK | 12.39±1.95a | -27.85 | 4.38±0.18a | -30.82 | 328.99±1.38a | -4.72 | 0.26±0.03a | -7.69 |
LN | 8.94±0.39b | 3.03±0.32b | 313.45±4.10a | 0.24±0.01a | |||||
5 | CK | 9.39±1.14a | -44.20 | 3.34±1.06a | -49.10 | 336.42±32.39a | -0.38 | 0.32±0.11a | -53.13 |
LN | 5.24±0.04b | 1.70±0.10b | 335.13±2.83a | 0.15±0.01b | |||||
6 | CK | 7.05±1.78a | -23.97 | 2.92±0.26a | -62.67 | 349.66±7.19a | -15.91 | 0.26±0.03a | -65.38 |
LN | 5.36±0.90b | 1.09±0.06b | 294.04±23.24b | 0.09±0.01b | |||||
7 | CK | 10.69±3.21a | -56.59 | 3.98±1.26a | -32.91 | 370.27±12.45a | -14.01 | 0.39±0.15a | -41.03 |
LN | 4.64±0.25b | 2.67±0.62b | 318.39±4.38b | 0.23±0.06b | |||||
8 | CK | 7.72±2.15a | -52.85 | 3.71±0.67a | -71.43 | 355.40±4.38a | -10.01 | 0.34±0.08a | -76.47 |
LN | 3.64±0.73b | 1.06±0.17b | 319.81±26.79b | 0.08±0.01b | |||||
9 | CK | 4.77±0.67a | -13.00 | 2.06±0.12a | -37.86 | 354.25±12.60a | -9.32 | 0.18±0.01a | -38.89 |
LN | 4.15±1.31a | 1.28±0.03b | 321.24±9.35a | 0.11±0.00b | |||||
10 | CK | 5.76±0.98a | -12.15 | 2.31±2.04a | -37.23 | 324.94±26.20a | -0.57 | 0.21±0.21a | -42.86 |
LN | 5.06±2.03a | 1.45±0.09b | 323.09±41.44a | 0.12±0.01b | |||||
11 | CK | 4.24±1.52a | -12.97 | 1.37±0.24a | -29.93 | 332.04±12.13a | -5.75 | 0.11±0.02a | -27.27 |
LN | 3.69±0.47a | 0.96±0.08b | 312.95±7.67a | 0.08±0.01b | |||||
12 | CK | 8.61±0.24a | -31.71 | 1.75±0.12a | -17.14 | 309.24±2.86a | -3.57 | 0.15±0.01a | -26.67 |
LN | 5.88±0.95b | 1.45±0.27a | 298.21±8.52a | 0.11±0.02b | |||||
13 | CK | 9.94±0.03a | -18.61 | 2.72±0.11a | -31.25 | 325.69±3.66a | -6.23 | 0.24±0.01a | -37.50 |
LN | 8.09±2.13a | 1.87±0.44b | 305.40±1.54a | 0.15±0.04b | |||||
14 | CK | 7.09±2.93a | -44.57 | 1.51±0.75a | -41.06 | 297.28±7.27a | -0.33 | 0.13±0.07a | -46.15 |
LN | 3.93±1.20b | 0.89±0.31b | 296.31±14.26a | 0.07±0.02b | |||||
15 | CK | 8.78±0.13a | -10.82 | 4.76±0.05a | -57.77 | 339.46±1.09a | -6.70 | 0.28±0.00a | -35.71 |
LN | 7.83±1.09a | 2.01±0.49b | 316.72±26.10a | 0.18±0.05b | |||||
16 | CK | 9.47±0.24a | -64.52 | 2.68±0.08a | -77.61 | 298.54±2.36a | -10.75 | 0.16±0.00a | -75.00 |
LN | 3.36±0.73b | 0.60±0.13b | 266.45±4.66b | 0.04±0.01b | |||||
17 | CK | 6.78±0.52a | -62.24 | 1.54±0.25a | -62.34 | 303.70±14.75a | -2.41 | 0.12±0.01a | -66.67 |
LN | 2.56±0.41b | 0.58±0.05b | 296.39±9.02a | 0.04±0.00b | |||||
18 | CK | 6.03±0.78a | -3.32 | 1.30±0.06a | -36.92 | 293.10±6.50a | -18.27 | 0.10±0.01a | -40.00 |
LN | 5.83±0.51a | 0.82±0.06b | 239.54±3.85b | 0.06±0.00b |
图1 不同皮燕麦品种的硝态氮含量在低氮胁迫下的变化1:蒙燕1号Mengyan No.1;2:青引1号Qingyin No.1;3:枪手Shooter;4:燕王Forage plus;5:青燕2号Qingyan No.2;6:边锋Blade;7:骏马Cayuse;8:莫妮卡Monica;9:甜燕70 Sweet oat 70;10:太阳神Titan;11:青海444 Qinghai 444;12:美达Monida;13:魅力Charisma;14:领袖Souris;15:青海甜燕麦Qinghai sweet oat;16:牧王Heymaker;17:甜燕2号Haywire;18:青燕1号Qingyan No.1. *表示同一品种的对照组与处理组在P<0.05水平显著相关,**表示同一品种的对照组与处理组在P<0.01水平极显著相关, ***表示同一品种的对照组与处理组在P<0.001水平极显著相关,下同。* means significant correlation at the level of P<0.05, ** means extremely significant correlation at the level of P<0.01, *** means extremely significant correlation at the level of P<0.001, the same below.
Fig.1 Changes of nitrate nitrogen content in different oat cultivars under low-nitrogen stress
品种 Cultivar | 株高 PH | 根长 RL | 根冠比 RSR | 地上部鲜重 SFW | 地下部鲜重 RFW | 地上部干重 SDW | 地下部干重 RDW | 净光合速率 Pn | 蒸腾速率 Tr | 胞间CO2浓度 Ci | 气孔导度 Gs | 硝态氮 NO3--N | 硝酸还原酶 NR | 谷氨酰胺合成酶 GS | 谷氨酸合成酶 GOGAT | 谷氨酸脱氢酶 GDH | 超氧阴离子 O2·- | 过氧化氢 H2O2 | 丙二醛 MDA | 可溶性蛋白 SP | 可溶性糖 SS |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 0.974 | 0.718 | 1.200 | 0.694 | 0.819 | 0.800 | 1.000 | 0.409 | 0.658 | 0.913 | 0.647 | 0.672 | 0.403 | 0.555 | 1.806 | 0.649 | 1.626 | 1.048 | 1.402 | 0.617 | 0.920 |
2 | 0.877 | 0.564 | 0.778 | 0.530 | 0.111 | 0.633 | 0.444 | 0.546 | 0.626 | 0.896 | 0.889 | 0.239 | 0.834 | 0.803 | 3.038 | 0.805 | 1.883 | 1.049 | 1.047 | 0.934 | 0.810 |
3 | 0.788 | 0.827 | 0.636 | 0.757 | 0.714 | 1.000 | 0.667 | 0.628 | 0.374 | 0.995 | 0.588 | 0.593 | 0.559 | 0.449 | 1.340 | 0.684 | 1.054 | 1.098 | 1.387 | 0.763 | 0.938 |
4 | 0.916 | 0.890 | 0.800 | 0.605 | 0.516 | 0.577 | 0.429 | 0.722 | 0.692 | 0.953 | 0.923 | 0.344 | 0.552 | 1.056 | 1.486 | 0.947 | 1.346 | 1.160 | 1.257 | 0.766 | 0.599 |
5 | 0.973 | 0.452 | 0.667 | 0.487 | 0.324 | 0.553 | 0.333 | 0.558 | 0.509 | 0.996 | 0.469 | 0.545 | 0.835 | 0.490 | 2.097 | 0.624 | 1.515 | 1.707 | 2.826 | 0.784 | 0.483 |
6 | 0.931 | 1.252 | 1.800 | 0.796 | 1.608 | 0.829 | 1.500 | 0.760 | 0.373 | 0.841 | 0.346 | 0.337 | 0.674 | 0.385 | 1.757 | 0.796 | 1.258 | 1.398 | 1.157 | 0.842 | 0.409 |
7 | 0.911 | 0.962 | 1.571 | 0.303 | 0.616 | 0.333 | 0.571 | 0.434 | 0.671 | 0.860 | 0.590 | 0.258 | 0.558 | 0.073 | 2.429 | 0.232 | 1.119 | 1.360 | 1.197 | 0.983 | 0.457 |
8 | 0.937 | 1.207 | 1.400 | 0.594 | 0.510 | 0.651 | 1.000 | 0.472 | 0.286 | 0.900 | 0.235 | 0.532 | 0.631 | 0.592 | 1.643 | 0.454 | 1.419 | 1.735 | 1.682 | 0.729 | 0.759 |
9 | 0.901 | 1.404 | 1.000 | 0.738 | 0.795 | 0.970 | 0.667 | 0.870 | 0.621 | 0.907 | 0.611 | 0.338 | 0.786 | 0.674 | 2.223 | 0.471 | 2.210 | 1.454 | 1.741 | 0.822 | 0.860 |
10 | 0.996 | 0.743 | 1.400 | 0.469 | 0.515 | 0.467 | 0.667 | 0.878 | 0.628 | 0.994 | 0.571 | 0.458 | 0.365 | 0.512 | 1.242 | 0.855 | 1.042 | 1.401 | 1.578 | 0.692 | 0.627 |
11 | 0.891 | 1.439 | 2.500 | 0.815 | 2.114 | 0.725 | 2.000 | 0.870 | 0.701 | 0.943 | 0.727 | 0.286 | 0.688 | 0.965 | 1.656 | 0.533 | 1.361 | 1.174 | 1.017 | 0.430 | 0.690 |
12 | 0.949 | 0.865 | 1.111 | 0.863 | 0.857 | 0.786 | 0.800 | 0.683 | 0.829 | 0.964 | 0.733 | 0.441 | 0.487 | 0.915 | 1.738 | 0.944 | 1.421 | 2.000 | 1.029 | 0.402 | 0.670 |
13 | 0.949 | 0.876 | 1.500 | 0.914 | 1.183 | 0.746 | 1.333 | 0.814 | 0.688 | 0.938 | 0.625 | 0.809 | 0.637 | 0.810 | 1.251 | 0.508 | 1.369 | 1.115 | 2.541 | 0.553 | 0.774 |
14 | 0.793 | 0.993 | 1.857 | 0.958 | 1.640 | 0.867 | 1.750 | 0.554 | 0.589 | 0.997 | 0.538 | 0.382 | 0.470 | 0.756 | 2.262 | 0.526 | 1.138 | 1.126 | 1.196 | 0.406 | 0.666 |
15 | 0.947 | 1.423 | 1.833 | 0.878 | 1.333 | 0.808 | 1.750 | 0.892 | 0.422 | 0.933 | 0.643 | 0.374 | 0.477 | 0.698 | 1.402 | 0.280 | 1.899 | 2.401 | 1.146 | 0.584 | 0.930 |
16 | 0.969 | 0.994 | 1.778 | 0.905 | 1.290 | 0.960 | 1.600 | 0.355 | 0.224 | 0.893 | 0.250 | 0.423 | 0.740 | 0.820 | 2.337 | 0.848 | 1.049 | 1.157 | 1.041 | 0.482 | 0.768 |
17 | 0.726 | 0.441 | 1.000 | 0.545 | 0.327 | 0.492 | 0.500 | 0.378 | 0.377 | 0.976 | 0.333 | 0.298 | 0.717 | 0.911 | 1.252 | 0.694 | 1.230 | 1.233 | 2.076 | 0.292 | 0.654 |
18 | 0.877 | 0.786 | 0.889 | 0.934 | 0.981 | 0.982 | 0.800 | 0.967 | 0.631 | 0.817 | 0.600 | 0.369 | 0.465 | 0.791 | 4.667 | 0.487 | 1.023 | 1.065 | 1.095 | 0.376 | 0.711 |
表4 18份皮燕麦材料各综合指标耐低氮系数
Table 4 The comprehensive index of 18 oat cultivars is resistant to low nitrogen coefficient
品种 Cultivar | 株高 PH | 根长 RL | 根冠比 RSR | 地上部鲜重 SFW | 地下部鲜重 RFW | 地上部干重 SDW | 地下部干重 RDW | 净光合速率 Pn | 蒸腾速率 Tr | 胞间CO2浓度 Ci | 气孔导度 Gs | 硝态氮 NO3--N | 硝酸还原酶 NR | 谷氨酰胺合成酶 GS | 谷氨酸合成酶 GOGAT | 谷氨酸脱氢酶 GDH | 超氧阴离子 O2·- | 过氧化氢 H2O2 | 丙二醛 MDA | 可溶性蛋白 SP | 可溶性糖 SS |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 0.974 | 0.718 | 1.200 | 0.694 | 0.819 | 0.800 | 1.000 | 0.409 | 0.658 | 0.913 | 0.647 | 0.672 | 0.403 | 0.555 | 1.806 | 0.649 | 1.626 | 1.048 | 1.402 | 0.617 | 0.920 |
2 | 0.877 | 0.564 | 0.778 | 0.530 | 0.111 | 0.633 | 0.444 | 0.546 | 0.626 | 0.896 | 0.889 | 0.239 | 0.834 | 0.803 | 3.038 | 0.805 | 1.883 | 1.049 | 1.047 | 0.934 | 0.810 |
3 | 0.788 | 0.827 | 0.636 | 0.757 | 0.714 | 1.000 | 0.667 | 0.628 | 0.374 | 0.995 | 0.588 | 0.593 | 0.559 | 0.449 | 1.340 | 0.684 | 1.054 | 1.098 | 1.387 | 0.763 | 0.938 |
4 | 0.916 | 0.890 | 0.800 | 0.605 | 0.516 | 0.577 | 0.429 | 0.722 | 0.692 | 0.953 | 0.923 | 0.344 | 0.552 | 1.056 | 1.486 | 0.947 | 1.346 | 1.160 | 1.257 | 0.766 | 0.599 |
5 | 0.973 | 0.452 | 0.667 | 0.487 | 0.324 | 0.553 | 0.333 | 0.558 | 0.509 | 0.996 | 0.469 | 0.545 | 0.835 | 0.490 | 2.097 | 0.624 | 1.515 | 1.707 | 2.826 | 0.784 | 0.483 |
6 | 0.931 | 1.252 | 1.800 | 0.796 | 1.608 | 0.829 | 1.500 | 0.760 | 0.373 | 0.841 | 0.346 | 0.337 | 0.674 | 0.385 | 1.757 | 0.796 | 1.258 | 1.398 | 1.157 | 0.842 | 0.409 |
7 | 0.911 | 0.962 | 1.571 | 0.303 | 0.616 | 0.333 | 0.571 | 0.434 | 0.671 | 0.860 | 0.590 | 0.258 | 0.558 | 0.073 | 2.429 | 0.232 | 1.119 | 1.360 | 1.197 | 0.983 | 0.457 |
8 | 0.937 | 1.207 | 1.400 | 0.594 | 0.510 | 0.651 | 1.000 | 0.472 | 0.286 | 0.900 | 0.235 | 0.532 | 0.631 | 0.592 | 1.643 | 0.454 | 1.419 | 1.735 | 1.682 | 0.729 | 0.759 |
9 | 0.901 | 1.404 | 1.000 | 0.738 | 0.795 | 0.970 | 0.667 | 0.870 | 0.621 | 0.907 | 0.611 | 0.338 | 0.786 | 0.674 | 2.223 | 0.471 | 2.210 | 1.454 | 1.741 | 0.822 | 0.860 |
10 | 0.996 | 0.743 | 1.400 | 0.469 | 0.515 | 0.467 | 0.667 | 0.878 | 0.628 | 0.994 | 0.571 | 0.458 | 0.365 | 0.512 | 1.242 | 0.855 | 1.042 | 1.401 | 1.578 | 0.692 | 0.627 |
11 | 0.891 | 1.439 | 2.500 | 0.815 | 2.114 | 0.725 | 2.000 | 0.870 | 0.701 | 0.943 | 0.727 | 0.286 | 0.688 | 0.965 | 1.656 | 0.533 | 1.361 | 1.174 | 1.017 | 0.430 | 0.690 |
12 | 0.949 | 0.865 | 1.111 | 0.863 | 0.857 | 0.786 | 0.800 | 0.683 | 0.829 | 0.964 | 0.733 | 0.441 | 0.487 | 0.915 | 1.738 | 0.944 | 1.421 | 2.000 | 1.029 | 0.402 | 0.670 |
13 | 0.949 | 0.876 | 1.500 | 0.914 | 1.183 | 0.746 | 1.333 | 0.814 | 0.688 | 0.938 | 0.625 | 0.809 | 0.637 | 0.810 | 1.251 | 0.508 | 1.369 | 1.115 | 2.541 | 0.553 | 0.774 |
14 | 0.793 | 0.993 | 1.857 | 0.958 | 1.640 | 0.867 | 1.750 | 0.554 | 0.589 | 0.997 | 0.538 | 0.382 | 0.470 | 0.756 | 2.262 | 0.526 | 1.138 | 1.126 | 1.196 | 0.406 | 0.666 |
15 | 0.947 | 1.423 | 1.833 | 0.878 | 1.333 | 0.808 | 1.750 | 0.892 | 0.422 | 0.933 | 0.643 | 0.374 | 0.477 | 0.698 | 1.402 | 0.280 | 1.899 | 2.401 | 1.146 | 0.584 | 0.930 |
16 | 0.969 | 0.994 | 1.778 | 0.905 | 1.290 | 0.960 | 1.600 | 0.355 | 0.224 | 0.893 | 0.250 | 0.423 | 0.740 | 0.820 | 2.337 | 0.848 | 1.049 | 1.157 | 1.041 | 0.482 | 0.768 |
17 | 0.726 | 0.441 | 1.000 | 0.545 | 0.327 | 0.492 | 0.500 | 0.378 | 0.377 | 0.976 | 0.333 | 0.298 | 0.717 | 0.911 | 1.252 | 0.694 | 1.230 | 1.233 | 2.076 | 0.292 | 0.654 |
18 | 0.877 | 0.786 | 0.889 | 0.934 | 0.981 | 0.982 | 0.800 | 0.967 | 0.631 | 0.817 | 0.600 | 0.369 | 0.465 | 0.791 | 4.667 | 0.487 | 1.023 | 1.065 | 1.095 | 0.376 | 0.711 |
图9 18份皮燕麦材料低氮胁迫下各指标耐低氮系数相关性分析PH:株高, plant height;RL:根长, root length;RSR:根冠比, root-shoot ratio;SFW:地上部鲜重, shoot fresh weight;RFW:地下部鲜重, root fresh weight;SDW:地上部干重, shoot dry weight;RDW:地下部干重, root dry weight;Pn:净光合速率, photosynthesis rate;Tr:气孔导度, transpiration rate;Ci:胞间CO2浓度, internal CO2 concentration;Gs:蒸腾速率, transpiration rate;NO3--N:硝态氮;NR:硝酸还原酶, nitrate reductase;GS:谷氨酰胺合成酶, glutamine synthetase;GOGAT:谷氨酸合成酶, glutamate synthase;GDH:谷氨酸脱氢酶, glutamate dehydrogenase; O2·-:超氧阴离子;H2O2:过氧化氢;MDA:丙二醛, malondialdehyde;SP:可溶性蛋白, soluble protein;SS:可溶性糖, soluble sugar。下同The same below。 * 表示在P<0.05水平显著相关,**表示在P<0.01水平极显著相关,***表示在P<0.001水平极显著相关。* means significant correlation at the level of P<0.05, ** means extremely significant correlation at the level of P<0.01. *** means extremely significant correlation at the level of P<0.001.
Fig.9 Correlation analysis of low-nitrogen tolerance coefficient of each index under low nitrogen stress of 18 oat cultivars
变量 Variable | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 | 主成分4 Principal component 4 | 主成分5 Principal component 5 | 主成分6 Principal component 6 | 主成分7 Principal component 7 |
---|---|---|---|---|---|---|---|
地下部干重Root dry weight | 0.878 | -0.247 | -0.241 | 0.035 | -0.128 | 0.051 | 0.010 |
地上部鲜重Shoot fresh weight | 0.846 | 0.293 | -0.215 | -0.001 | 0.238 | -0.112 | 0.123 |
地上部干重Shoot dry weight | 0.791 | 0.033 | 0.075 | -0.073 | -0.370 | 0.114 | 0.186 |
根长Root length | 0.739 | -0.480 | 0.199 | 0.220 | 0.046 | 0.165 | -0.018 |
根冠比Root-shoot ratio | 0.693 | -0.459 | -0.103 | -0.013 | -0.396 | 0.110 | -0.046 |
地下部鲜重Root fresh weight | 0.643 | 0.224 | -0.152 | -0.051 | 0.588 | -0.168 | 0.149 |
可溶性糖Soluble sugar | -0.491 | -0.458 | 0.443 | 0.154 | 0.131 | -0.026 | 0.191 |
谷氨酰胺合成酶Glutamine synthetase | 0.382 | 0.688 | -0.023 | -0.025 | -0.010 | 0.450 | 0.149 |
胞间二氧化碳浓度Internal CO2 concentration | -0.192 | 0.454 | -0.383 | 0.445 | -0.366 | 0.250 | -0.226 |
气孔导度Stomatal conductance | 0.033 | 0.529 | 0.749 | 0.178 | -0.125 | 0.047 | -0.101 |
蒸腾速率Transpiration rate | 0.005 | 0.441 | 0.665 | 0.160 | -0.329 | -0.171 | -0.083 |
净光合速率Photosynthesis rate | 0.462 | 0.141 | 0.467 | 0.284 | -0.035 | -0.130 | 0.060 |
谷氨酸合成酶Glutamate synthase | 0.042 | 0.034 | 0.403 | -0.604 | 0.446 | -0.279 | 0.027 |
过氧化氢H2O2 | 0.094 | -0.351 | 0.066 | 0.586 | -0.028 | 0.151 | -0.104 |
超氧阴离子O2·- | -0.001 | -0.037 | 0.418 | 0.555 | 0.500 | 0.407 | 0.008 |
可溶性蛋白Soluble protein | 0.297 | 0.337 | -0.100 | 0.347 | 0.599 | -0.062 | -0.294 |
硝酸还原酶Nitrate reductase | -0.272 | -0.177 | -0.068 | -0.089 | 0.396 | 0.649 | 0.376 |
硝态氮NO3--N | -0.051 | 0.217 | -0.476 | 0.531 | 0.096 | -0.556 | 0.180 |
谷氨酸脱氢酶Glutamate dehydrogenase | -0.168 | 0.532 | -0.088 | -0.161 | -0.247 | 0.144 | 0.591 |
株高Plant height | 0.038 | -0.279 | 0.244 | 0.463 | -0.136 | -0.360 | 0.585 |
丙二醛Malondialdehyde | -0.513 | 0.037 | -0.400 | 0.454 | 0.063 | 0.009 | 0.112 |
特征值Eigenvalue | 4.658 | 2.702 | 2.559 | 2.291 | 2.049 | 1.561 | 1.180 |
贡献率Contribution (%) | 22.181 | 12.869 | 12.187 | 10.907 | 9.755 | 7.433 | 5.620 |
累计贡献率Cumulative contribution (%) | 22.181 | 35.049 | 47.236 | 58.144 | 67.899 | 75.332 | 80.952 |
表5 各综合指标系数及贡献率
Table 5 Coefficients and proportion rates of various comprehensive indicators
变量 Variable | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 | 主成分4 Principal component 4 | 主成分5 Principal component 5 | 主成分6 Principal component 6 | 主成分7 Principal component 7 |
---|---|---|---|---|---|---|---|
地下部干重Root dry weight | 0.878 | -0.247 | -0.241 | 0.035 | -0.128 | 0.051 | 0.010 |
地上部鲜重Shoot fresh weight | 0.846 | 0.293 | -0.215 | -0.001 | 0.238 | -0.112 | 0.123 |
地上部干重Shoot dry weight | 0.791 | 0.033 | 0.075 | -0.073 | -0.370 | 0.114 | 0.186 |
根长Root length | 0.739 | -0.480 | 0.199 | 0.220 | 0.046 | 0.165 | -0.018 |
根冠比Root-shoot ratio | 0.693 | -0.459 | -0.103 | -0.013 | -0.396 | 0.110 | -0.046 |
地下部鲜重Root fresh weight | 0.643 | 0.224 | -0.152 | -0.051 | 0.588 | -0.168 | 0.149 |
可溶性糖Soluble sugar | -0.491 | -0.458 | 0.443 | 0.154 | 0.131 | -0.026 | 0.191 |
谷氨酰胺合成酶Glutamine synthetase | 0.382 | 0.688 | -0.023 | -0.025 | -0.010 | 0.450 | 0.149 |
胞间二氧化碳浓度Internal CO2 concentration | -0.192 | 0.454 | -0.383 | 0.445 | -0.366 | 0.250 | -0.226 |
气孔导度Stomatal conductance | 0.033 | 0.529 | 0.749 | 0.178 | -0.125 | 0.047 | -0.101 |
蒸腾速率Transpiration rate | 0.005 | 0.441 | 0.665 | 0.160 | -0.329 | -0.171 | -0.083 |
净光合速率Photosynthesis rate | 0.462 | 0.141 | 0.467 | 0.284 | -0.035 | -0.130 | 0.060 |
谷氨酸合成酶Glutamate synthase | 0.042 | 0.034 | 0.403 | -0.604 | 0.446 | -0.279 | 0.027 |
过氧化氢H2O2 | 0.094 | -0.351 | 0.066 | 0.586 | -0.028 | 0.151 | -0.104 |
超氧阴离子O2·- | -0.001 | -0.037 | 0.418 | 0.555 | 0.500 | 0.407 | 0.008 |
可溶性蛋白Soluble protein | 0.297 | 0.337 | -0.100 | 0.347 | 0.599 | -0.062 | -0.294 |
硝酸还原酶Nitrate reductase | -0.272 | -0.177 | -0.068 | -0.089 | 0.396 | 0.649 | 0.376 |
硝态氮NO3--N | -0.051 | 0.217 | -0.476 | 0.531 | 0.096 | -0.556 | 0.180 |
谷氨酸脱氢酶Glutamate dehydrogenase | -0.168 | 0.532 | -0.088 | -0.161 | -0.247 | 0.144 | 0.591 |
株高Plant height | 0.038 | -0.279 | 0.244 | 0.463 | -0.136 | -0.360 | 0.585 |
丙二醛Malondialdehyde | -0.513 | 0.037 | -0.400 | 0.454 | 0.063 | 0.009 | 0.112 |
特征值Eigenvalue | 4.658 | 2.702 | 2.559 | 2.291 | 2.049 | 1.561 | 1.180 |
贡献率Contribution (%) | 22.181 | 12.869 | 12.187 | 10.907 | 9.755 | 7.433 | 5.620 |
累计贡献率Cumulative contribution (%) | 22.181 | 35.049 | 47.236 | 58.144 | 67.899 | 75.332 | 80.952 |
品种 Cultivar | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 | 主成分4 Principal component 4 | 主成分5 Principal component 5 | 主成分6 Principal component 6 | 主成分7 Principal component 7 | U(X1) | U(X2) | U(X3) | U(X4) | U(X5) | U(X6) | U(X7) | D值 D values | 耐低氮性排序 Low nitrogen tolerance ranking |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | -0.319 | 0.886 | -0.210 | 0.909 | 0.537 | -1.960 | -0.125 | 0.478 | 0.777 | 0.480 | 0.674 | 0.584 | 0.084 | 0.395 | 0.524 | 9 |
2 | -2.501 | 1.079 | 2.535 | -1.117 | 1.835 | 1.454 | 0.293 | 0.201 | 0.812 | 1.000 | 0.308 | 0.828 | 0.839 | 0.518 | 0.590 | 6 |
3 | -0.752 | 1.285 | -1.709 | 0.059 | 1.265 | -0.858 | -1.049 | 0.423 | 0.848 | 0.195 | 0.520 | 0.721 | 0.328 | 0.124 | 0.476 | 13 |
4 | -0.550 | 2.140 | 1.841 | -0.146 | -1.835 | 0.951 | 1.026 | 0.449 | 1.000 | 0.868 | 0.483 | 0.138 | 0.728 | 0.733 | 0.613 | 5 |
5 | -4.087 | -0.500 | -0.851 | 1.451 | 0.096 | 0.269 | 1.157 | 0.000 | 0.531 | 0.358 | 0.771 | 0.501 | 0.577 | 0.772 | 0.410 | 16 |
6 | 1.335 | -2.734 | -0.031 | -1.243 | -0.587 | 0.057 | 1.934 | 0.688 | 0.135 | 0.513 | 0.286 | 0.373 | 0.531 | 1.000 | 0.489 | 12 |
7 | -2.634 | -3.493 | 1.981 | -1.375 | -1.391 | -0.952 | -1.471 | 0.184 | 0.000 | 0.895 | 0.262 | 0.222 | 0.307 | 0.000 | 0.276 | 18 |
8 | -0.633 | -2.392 | -1.575 | 0.752 | 0.880 | -0.042 | -0.111 | 0.438 | 0.195 | 0.221 | 0.645 | 0.649 | 0.509 | 0.399 | 0.424 | 14 |
9 | 0.369 | -0.426 | 1.785 | 1.456 | 2.747 | 1.205 | 0.056 | 0.565 | 0.544 | 0.858 | 0.772 | 1.000 | 0.784 | 0.448 | 0.699 | 1 |
10 | -1.649 | 0.210 | 0.212 | 1.018 | -2.569 | -1.348 | 0.094 | 0.309 | 0.657 | 0.560 | 0.693 | 0.000 | 0.220 | 0.460 | 0.419 | 15 |
11 | 3.799 | -0.077 | 0.822 | -0.224 | -1.682 | 1.642 | -0.200 | 1.000 | 0.606 | 0.675 | 0.469 | 0.167 | 0.881 | 0.373 | 0.663 | 3 |
12 | 0.606 | 2.080 | 0.810 | 0.930 | -1.056 | -0.070 | 0.387 | 0.595 | 0.989 | 0.673 | 0.677 | 0.285 | 0.502 | 0.546 | 0.632 | 4 |
13 | 0.727 | 1.105 | -1.168 | 2.110 | -0.097 | -1.145 | 0.743 | 0.610 | 0.816 | 0.298 | 0.890 | 0.465 | 0.265 | 0.650 | 0.588 | 7 |
14 | 2.408 | 0.793 | -1.264 | -1.226 | -0.977 | 0.127 | -1.435 | 0.824 | 0.761 | 0.280 | 0.289 | 0.299 | 0.546 | 0.011 | 0.515 | 10 |
15 | 3.159 | -1.548 | 0.614 | 2.718 | 0.656 | 0.579 | -1.442 | 0.919 | 0.345 | 0.636 | 1.000 | 0.607 | 0.646 | 0.009 | 0.671 | 2 |
16 | 1.795 | -0.758 | -2.480 | -1.813 | 0.808 | 0.251 | 1.864 | 0.746 | 0.486 | 0.049 | 0.183 | 0.635 | 0.573 | 0.979 | 0.511 | 11 |
17 | -2.591 | 1.090 | -2.739 | -1.435 | -0.332 | 2.180 | -1.463 | 0.190 | 0.814 | 0.000 | 0.251 | 0.421 | 1.000 | 0.002 | 0.358 | 17 |
18 | 1.519 | 1.261 | 1.425 | -2.826 | 1.701 | -2.342 | -0.259 | 0.711 | 0.844 | 0.790 | 0.000 | 0.803 | 0.000 | 0.356 | 0.570 | 8 |
权重Weight | 0.274 | 0.159 | 0.151 | 0.135 | 0.121 | 0.092 | 0.069 |
表6 18份皮燕麦材料各综合指标值、权重、隶属函数值、D值及综合评价
Table 6 The comprehensive indicator values, index weight, membership function value, D values and comprehensive evaluation of 18 oat cultivars
品种 Cultivar | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 | 主成分4 Principal component 4 | 主成分5 Principal component 5 | 主成分6 Principal component 6 | 主成分7 Principal component 7 | U(X1) | U(X2) | U(X3) | U(X4) | U(X5) | U(X6) | U(X7) | D值 D values | 耐低氮性排序 Low nitrogen tolerance ranking |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | -0.319 | 0.886 | -0.210 | 0.909 | 0.537 | -1.960 | -0.125 | 0.478 | 0.777 | 0.480 | 0.674 | 0.584 | 0.084 | 0.395 | 0.524 | 9 |
2 | -2.501 | 1.079 | 2.535 | -1.117 | 1.835 | 1.454 | 0.293 | 0.201 | 0.812 | 1.000 | 0.308 | 0.828 | 0.839 | 0.518 | 0.590 | 6 |
3 | -0.752 | 1.285 | -1.709 | 0.059 | 1.265 | -0.858 | -1.049 | 0.423 | 0.848 | 0.195 | 0.520 | 0.721 | 0.328 | 0.124 | 0.476 | 13 |
4 | -0.550 | 2.140 | 1.841 | -0.146 | -1.835 | 0.951 | 1.026 | 0.449 | 1.000 | 0.868 | 0.483 | 0.138 | 0.728 | 0.733 | 0.613 | 5 |
5 | -4.087 | -0.500 | -0.851 | 1.451 | 0.096 | 0.269 | 1.157 | 0.000 | 0.531 | 0.358 | 0.771 | 0.501 | 0.577 | 0.772 | 0.410 | 16 |
6 | 1.335 | -2.734 | -0.031 | -1.243 | -0.587 | 0.057 | 1.934 | 0.688 | 0.135 | 0.513 | 0.286 | 0.373 | 0.531 | 1.000 | 0.489 | 12 |
7 | -2.634 | -3.493 | 1.981 | -1.375 | -1.391 | -0.952 | -1.471 | 0.184 | 0.000 | 0.895 | 0.262 | 0.222 | 0.307 | 0.000 | 0.276 | 18 |
8 | -0.633 | -2.392 | -1.575 | 0.752 | 0.880 | -0.042 | -0.111 | 0.438 | 0.195 | 0.221 | 0.645 | 0.649 | 0.509 | 0.399 | 0.424 | 14 |
9 | 0.369 | -0.426 | 1.785 | 1.456 | 2.747 | 1.205 | 0.056 | 0.565 | 0.544 | 0.858 | 0.772 | 1.000 | 0.784 | 0.448 | 0.699 | 1 |
10 | -1.649 | 0.210 | 0.212 | 1.018 | -2.569 | -1.348 | 0.094 | 0.309 | 0.657 | 0.560 | 0.693 | 0.000 | 0.220 | 0.460 | 0.419 | 15 |
11 | 3.799 | -0.077 | 0.822 | -0.224 | -1.682 | 1.642 | -0.200 | 1.000 | 0.606 | 0.675 | 0.469 | 0.167 | 0.881 | 0.373 | 0.663 | 3 |
12 | 0.606 | 2.080 | 0.810 | 0.930 | -1.056 | -0.070 | 0.387 | 0.595 | 0.989 | 0.673 | 0.677 | 0.285 | 0.502 | 0.546 | 0.632 | 4 |
13 | 0.727 | 1.105 | -1.168 | 2.110 | -0.097 | -1.145 | 0.743 | 0.610 | 0.816 | 0.298 | 0.890 | 0.465 | 0.265 | 0.650 | 0.588 | 7 |
14 | 2.408 | 0.793 | -1.264 | -1.226 | -0.977 | 0.127 | -1.435 | 0.824 | 0.761 | 0.280 | 0.289 | 0.299 | 0.546 | 0.011 | 0.515 | 10 |
15 | 3.159 | -1.548 | 0.614 | 2.718 | 0.656 | 0.579 | -1.442 | 0.919 | 0.345 | 0.636 | 1.000 | 0.607 | 0.646 | 0.009 | 0.671 | 2 |
16 | 1.795 | -0.758 | -2.480 | -1.813 | 0.808 | 0.251 | 1.864 | 0.746 | 0.486 | 0.049 | 0.183 | 0.635 | 0.573 | 0.979 | 0.511 | 11 |
17 | -2.591 | 1.090 | -2.739 | -1.435 | -0.332 | 2.180 | -1.463 | 0.190 | 0.814 | 0.000 | 0.251 | 0.421 | 1.000 | 0.002 | 0.358 | 17 |
18 | 1.519 | 1.261 | 1.425 | -2.826 | 1.701 | -2.342 | -0.259 | 0.711 | 0.844 | 0.790 | 0.000 | 0.803 | 0.000 | 0.356 | 0.570 | 8 |
权重Weight | 0.274 | 0.159 | 0.151 | 0.135 | 0.121 | 0.092 | 0.069 |
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