The identification and expression analysis of genes related to seed germination in Leymus chinensis

doi:10.11686/cyxb2017502

Abstract

Abstract: Previous studies have suggested that the variable temperature (28 ℃ 12 h/16 ℃ 12 h) can significantly increase the germination rate of Leymus chinensis seed, and that the first day of germination was key for responses to variable temperature. A transcriptome analysis of L. chinensis seed germination under variable temperature treatment was performed. Based on sequencing, 24 genes related to seed germination, low temperature and dormancy were selected for heat map analysis using the reads per kilo base per million mapped reads (RPKM) value. In addition, under the treatment of constant temperature (28 ℃) for 12 h and variable temperature (28 ℃ 12 h/16 ℃ 12 h) for 1 d, the relative expression of the selected genes in two L. chinensis genotypes (high and low germination rate) were quantified by qRT-PCR. Our results indicated that the expression of SAIN1, PP2C62, EXPB3, EXPB4, GA3ox, EXPA2 and EXPA7 were significantly up-regulated in the seeds treated at 16 ℃ for 12 h, while the expression of bHLH49, GID1, ABI8, Chi1, 11833, CBF3, NAC2, PP2C72, SAIN2 and 5423 were significantly down-regulated, compared with the constant temperature (28 ℃) control. Further analysis of the gene expression in the two genotypes suggested that Chi1, CBF3, GA3ox, EXPB4, 5423 and SAIN1 were the putative genes related to germination in L. chinensis.This research may lay the foundation for further studies on the molecular mechanisms in seed germination of L. chinensis.

Key words: Leymus chinensis, seed germination, variable temperature treatment, gene expression

LIU Shu, LIU Pan-pan, YANG Wei-guang, QI Dong-mei, LI Xiao-xia, LIU Gong-she. The identification and expression analysis of genes related to seed germination in Leymus chinensis[J]. Acta Prataculturae Sinica, 2018, 27(11): 58-66.

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