Arbuscular mycorrhizal fungi (AMF) enhance phytoremediation of strontium-contaminated soil by Sorghum bicolor seedlings

doi:10.11686/cyxb2018333

Abstract

Abstract: In this study, we determined the effects of arbuscular mycorrhizal fungi (AMF) on the ability of Sorghum bicolor to remediate soil polluted with strontium (Sr). Seedlings of S. bicolor were inoculated with Glomus geosporum, Glomus mosseae, Glomus versiforme, or Glomus diaphanum, to compare the effects of different AMF on the strontium accumulation capacity of S. bicolor. All the AMF had infection rates of >50%, and G. versiforme showed the highest infection rate (75%). The largest biomass increases were in plants inoculated with G. geosporum and G. mosseae. The shoot biomass and root biomass of seedlings inoculated with G. geosporum increased by 54.72% and 53.26%, respectively, compared with uninoculated seedlings. Sorghum seedlings showed significant mycorrhizal dependency (P<0.05), suggesting that AMF promoted their growth. Among the AMF, G. geosporum and G. mosseae significantly increased the biomass of sorghum seedlings, and the mycorrhizal dependencies of sorghum for these two AMF were 154.44% and 147.61%, respectively. The shoot and root Sr concentrations were significantly higher (P<0.05) in AMF-inoculated sorghum than in uninoculated seedlings, and the translocation factors of AMF-inoculated sorghum were all >1. The Sr concentrations were 30.18%-86.05% higher in G. geosporum-inoculated sorghum seedlings than in uninoculated seedlings. The total phosphorus and available phosphorus contents in sorghum seedlings were significantly (P<0.05) decreased by inoculation with G. geosporum, G. mosseae, and G. versiforme; the total phosphorus content was decreased by 15.89%-20.32%, and the available phosphorus content was decreased by 12.98%-18.49%. The activity of soil phosphatase was 21.43%-30.36% higher in AMF treatments than in controls (P<0.05). The soil invertase activities were 25.77%-28.87% higher in the G. geosporum, G. mosseae, and G. versiforme treatments than in controls. In conclusion, AMF enhanced the ability of S. bicolor to accumulate and tolerate Sr, and G. geosporum was the most effective AMF.

Key words: strontium, arbuscular mycorrhizal fungi, Sorghum bicolor, phytoremediation

QI Lin, YANG Ying-bo, ZHANG Bo, ZHAO Wei, WANG Xiao-ling, LIU Yu-hua. Arbuscular mycorrhizal fungi (AMF) enhance phytoremediation of strontium-contaminated soil by Sorghum bicolor seedlings[J]. Acta Prataculturae Sinica, 2018, 27(12): 103-112.

References

[1] Kato M, Okada Y, Hirai S, et al. Comparative analysis of distributions of radioactive cesium and potassium and stable cesium, potassium, and strontium in brown rice grains contaminated with radioactive materials released by the Fukushima Daiichi Nuclear Power Plant accident. Journal of Radioanalytical and Nuclear Chemistry, 2016, 310(1): 247-252.
[2] Yu R L, Hu G R.Research progress in sources identification of soil heavy metal pollution. Nonferrous Metals, 2008, 60(4): 158-165.
于瑞莲, 胡恭任. 土壤中重金属污染源解析研究进展. 有色金属工程, 2008, 60(4): 158-165.
[3] Wu M J.The relationship between strontium and human health. Studies of Trace Elements and Health, 2012, 29(5): 66-67.
吴茂江. 锶与人体健康. 微量元素与健康研究, 2012, 29(5): 66-67.
[4] Tang S R, Shang Z R, Song Z G, et al. Some issues related to cleanup standards for remediation of radionuclide contaminated soils. Journal of Agro-Environment Science, 2007, 26(2): 407-412.
唐世荣, 商照荣, 宋正国, 等. 放射性核素污染土壤修复标准的若干问题. 农业环境科学学报, 2007, 26(2): 407-412.
[5] Annual report of the national nuclear safety administration of the People’s Republic of China. Beijing: Ministry of Ecology and Environment of the People’s Republic of China, 2017.
中华人民共和国国家核安全局2017年年度报告. 北京: 中华人民共和国生态环境部, 2017.
[6] Tsukada H, Takeda A, Takahashi T, et al. Uptake and distribution of ⁹⁰Sr and stable Sr in rice plants. Journal of Environmental Radioactivity, 2005, 81(2): 221-231.
[7] Wei C Y, Chen T B.Hyperaccumulators and phytoremediation of heavy metal comtaminated soil: A review of studies in China and abroad. Acta Ecologica Sinica, 2001, 21(7): 1196-1203.
韦朝阳, 陈同斌. 重金属超富集植物及植物修复技术研究进展. 生态学报, 2001, 21(7): 1196-1203.
[8] Prasad M, Freitas H.Metal hyperaccumulation in plants-biodiversity prospecting for phytoremediation technology. Electronic Journal of Biotechnology, 2003, 6(3): 285-321.
[9] Tang Y J, Luo X G, Zeng F, et al. The responses of plants to high concentrations of strontium, cesium stress and the screening of remediation plants. Journal of Agro-Environment Science, 2013, 32(5): 960-965.
唐永金, 罗学刚, 曾峰, 等. 不同植物对高浓度Sr、Cs胁迫的响应与修复植物筛选. 农业环境科学学报, 2013, 32(5): 960-965.
[10] Fuhrmann M, Lasat M M, Ebbs S D, et al. Uptake of cesium-137 and strontium-90 from contaminated soil by three plant species; application to phytoremediation. Journal of Environmental Quality, 2002, 31(3): 904-909.
[11] Zhang X X, Wang D, Li W F, et al. Studies on accumulation of ¹³³Cs and ⁸⁸Sr in Vicia faba Linn and irradiation damage effect. Journal of Radioactivity Research and Radioactivity Process, 2010, 28(1): 48-52.
张晓雪, 王丹, 李卫锋, 等. ¹³³Cs和⁸⁸Sr在蚕豆苗中的蓄积及其辐射损伤效应. 辐射研究与辐射工艺学报, 2010, 28(1): 48-52.
[12] Xiang D, Xu T L, Li H, et al. Ecological distribution of arbuscular mycorrhizal fungi and the influencing factors. Acta Ecologica Sinica, 2017, 37(11): 3597-3606.
向丹, 徐天乐, 李欢, 等. 丛枝菌根真菌的生态分布及其影响因子研究进展. 生态学报, 2017, 37(11): 3597-3606.
[13] Chen B D, Chen M M, Bai R.Potential role of arbuscular mycorrhiza in bioremediation of uranium contaminated environments. Environmental Science, 2011, 32(3): 809-816.
陈保冬, 陈梅梅, 白刃. 丛枝菌根在治理铀污染环境中的潜在作用. 环境科学, 2011, 32(3): 809-816.
[14] Zhuang P, Shu W S, Li Z A, et al. Removal of metals by sorghum plants from contaminated land. Journal of Environmental Sciences, 2009, 21(10): 1432-1437.
[15] Wang X, Chen C, Wang J.Phytoremediation of strontium contaminated soil by Sorghum bicolor (L.) Moench and soil microbial community-level physiological profiles (CLPPs). Environmental Science and Pollution Research, 2017, 24(8): 7668-7678.
[16] Chen L H, Hu X W, Yang W Q, et al. Effect of arbuscular mycorrhizae fungi inoculation on absorption of Pb and Cd in females and males of Populus deltoids when exposed to Pb and Cd pollution. Acta Scientiae Circumstantiae, 2017, 37(1): 308-317.
陈良华, 胡相伟, 杨万勤, 等. 接种丛枝菌根真菌对雌雄美洲黑杨吸收铅镉的影响. 环境科学学报, 2017, 37(1): 308-317.
[17] Fu X F, Zhang G P, Zhang X W, et al. Effect of PSB and AMF on growth, microorganisms and soil enzyme activities in the rhizosphere of Taxus chinensis var. mairei seedlings. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(2): 353-360.
付晓峰, 张桂萍, 张小伟, 等. 溶磷细菌和丛枝菌根真菌接种对南方红豆杉生长及根际微生物和土壤酶活性的影响. 西北植物学报, 2016, 36(2): 353-360.
[18] Yang H L, Huang R H, Chen K, et al. The effects of arbuscular mycorrhizal fungi (AMF) on growth of Sorghum haipense and rhizosphere soil enzymes activities under Cs stress. Environmental Chemistry, 2015, 34(4): 712-717.
杨会玲, 黄仁华, 陈珂, 等. 丛枝菌根真菌(AMF)对铯胁迫宿根高粱生长及根际土壤酶的影响. 环境化学, 2015, 34(4): 712-717.
[19] Hou S J, Li T, Lin G, et al. The influence of biogas residue and arbuscular mycorrhizal fungi on growth and mineral nutrition of Glycyrrhiza uralensis. Acta Scientiae Circumstantiae, 2016, 36(12): 4453-4460.
侯时季, 李涛, 蔺阁, 等. 施用沼渣和接种丛枝菌根真菌对甘草生长及矿质营养的影响. 环境科学学报, 2016, 36(12): 4453-4460.
[20] Yuan L H, Yan G Q.Rhizospheric soil of seedlingss of Elaeagnus mollis colonized by arbuscular mycorrhizal fungi. Chinese Journal of Plant Ecology, 2010, 34(6): 678-686.
袁丽环, 闫桂琴. 丛枝菌根化翅果油树幼苗根际土壤微环境. 植物生态学报, 2010, 34(6): 678-686.
[21] Lu R K.Analysis methods for soil and agrochemistry. Beijing: Chinese Agricultural Science and Technology Press, 2000.
鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000.
[22] Phillips J M, Hayman D S.Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 1970, 55(1): 158-161.
[23] Liu Z, Jie L I, Yang Y, et al. Research and application of microwave assisted digestion procedure for the determination of 23 elements in sediments by ICP-AES/ICP-MS. Environmental Chemistry, 2013, 32(12): 2370-2377.
[24] Soudek P, Valenová Š, Vavríková Z, et al. ¹³⁷Cs and ⁹⁰Sr uptake by sunflower cultivated under hydroponic conditions. Journal of Environmental Radioactivity, 2006, 88(3): 236-250.
[25] Guan S Y.Soil enzyme and its research methods. Beijing: Agricultural Publishing House, 1986.
关松荫. 土壤酶及其研究法. 北京: 农业出版社, 1986.
[26] Wu S L, Zhang X, Chen B D.Effects of arbuscular mycorrhizal fungi on heavy metal translocation and transformation in the soil-plant continuum. Asian Journal of Ecotoxicology, 2013, 8(6): 847-856.
伍松林, 张莘, 陈保冬. 丛枝菌根对土壤-植物系统中重金属迁移转化的影响. 生态毒理学报, 2013, 8(6): 847-856.
[27] Wei L L, Lu C Y, Ding J, et al. Functional relationships between arbuscular mycorrhizal symbionts and nutrient dynamics in plant-soil-microbe system. Acta Ecologica Sinica, 2016, 36(14): 4233-4243.
韦莉莉, 卢昌熠, 丁晶, 等. 丛枝菌根真菌参与下植物—土壤系统的养分交流及调控. 生态学报, 2016, 36(14): 4233-4243.
[28] Huang R H, Yang H L, Huang W, et al. Effects of Funneliformis mosseae on endogenous hormones and photosynthesis of Sorghum haipense under Cs stress. Chinese Journal of Applied Ecology, 2015, 26(7): 2146-2150.
黄仁华, 杨会玲, 黄炜, 等. 核素铯胁迫下接种摩西球囊霉对宿根高粱内源激素和光合的影响. 应用生态学报, 2015, 26(7): 2146-2150.
[29] Song Y C, Feng G, Li X L.Effect of vam fungi on phosphatese activity in the rhizosphere of clover. Chinese Journal of Applied & Environmental Biology, 2000, 6(2): 171-175.
宋勇春, 冯固, 李晓林. 泡囊丛枝菌根对红三叶草根际土壤磷酸酶活性的影响. 应用与环境生物学报, 2000, 6(2): 171-175.
[30] He Y J, Zhong Z C, Dong M.Nutrients transfer for host plant and litter decompositon by AMF in Karst soil. Acta Ecologica Sinica, 2012, 32(8): 2525-2531.
何跃军, 钟章成, 董鸣. AMF对喀斯特土壤枯落物分解和对宿主植物的养分传递. 生态学报, 2012, 32(8): 2525-2531.
[31] Wu H M, Li F L, Mou H Q, et al. Analysis of heavy metal fractions in plants by two steps sequential extraction procedure. Environmental Science and Technology, 2012, 35(7): 133-137.
吴慧梅, 李非里, 牟华倩, 等. 两步连续提取法测定植物中重金属的形态. 环境科学与技术, 2012, 35(7): 133-137.
[32] Huang J, Ling W T, Sun Y D, et al. Impacts of arbuscular mycorrhizal fungi inoculation on the uptake of cadmium and zinc by alfalfa in contaminated soil. Journal of Agro-Environment Science, 2012, 31(1): 99-105.
黄晶, 凌婉婷, 孙艳娣, 等. 丛枝菌根真菌对紫花苜蓿吸收土壤中镉和锌的影响. 农业环境科学学报, 2012, 31(1): 99-105.
[33] Li X, Peng X W, Wu S L, et al. Effect of arbuscular mycorrhizae on growth, heavy metal uptake and accumulation of Zenia insignis chun seedlingss. Environmental Science, 2014, 1(8): 3142-3148.
李霞, 彭霞薇, 伍松林, 等. 丛枝菌根对翅荚木生长及吸收累积重金属的影响. 环境科学, 2014, 1(8): 3142-3148.
[34] Wang X, Xie L K, Wu H B, et al. Effect of elevated CO₂ on indica rice quality in soils combined with Cu and Cd heavy metal. Acta Ecologica Sinica, 2015, 35(17): 5728-5737.
王潇, 谢丽坤, 武慧斌, 等. 浓度升高对籼稻稻米品质的影响. 生态学报, 2015, 35(17): 5728-5737.
[35] Sun J H, Bi Y L, Qiu L, et al. A review about the effect of AMF on uptaking phosphorus by host plants in soil. Chinese Journal of Soil Science, 2016, 47(2): 499-504.
孙金华, 毕银丽, 裘浪, 等. 土壤中丛枝菌根真菌对宿主植物磷吸收作用机制综述. 土壤通报, 2016, 47(2): 499-504.
[36] Song F Q, Yang G T, Meng F R, et al. The rhizospheric niche of seedlingss of Populus ussruiensis colonized by arbuscular mycorrhizal (AM) fungi. Ecology and Environment, 2004, 13(2): 211-216.
宋福强, 杨国亭, 孟繁荣, 等. 丛枝菌根化大青杨苗木根际微域环境的研究. 生态环境学报, 2004, 13(2): 211-216.
[37] Zu Y Q, Lu X, Zhan F D, et al. A review on roles and mechanisms of arbuscular mycorrhizal fungi in phytoremediation of heavy metals-polluted soils. Plant Physiology Journal, 2015, 51(10): 1538-1548.
祖艳群, 卢鑫, 湛方栋, 等. 丛枝菌根真菌在土壤重金属污染植物修复中的作用及机理研究进展. 植物生理学报, 2015, 51(10): 1538-1548.