Abstract: In order to study the biological function of flax heterogeneous acetyl-CoA carboxylase BCCP, BC, α-CT and β-CT genes, we used the gene cloning technology RT-PCR and bioinformatics analysis, to analyze four genes of flax accA, accB, accC and accD. It was found that the accA gene of Longya No. 10 encodes the α-CT subunit, and the CDS sequence is 2364 bp in length and encodes 787 amino acids; the accB gene encodes the BCCP subunit, and the CDS sequence is 1173 bp in length and encodes 275 amino acids; the accC gene encodes the BC subunit, and the CDS sequence is 1574 bp in length and encodes 527 amino acids; the accD gene encodes a β-CT subunit, and the CDS sequence is 1137 bp in length and encodes 378 amino acids. The proteins encoded by these four genes are all hydrophilic proteins with high fatty acid coefficients. RT-PCR results show that in different tissues of the three flax varieties, Zhangya No.2 with high oil content, Longya No.10 with medium oil content, and R 2-17 with low oil content, the four genes of flax accA, accB, accC, accD have different expression modes. During the rapid oil accumulation period of seed development (10-25 d), the expression levels of these four genes increased rapidly and reached the highest peak. Studies have shown that the heterogeneous acetyl-CoA carboxylase genes may regulate the accumulation of lipid in flax seeds during early development.

Key words: flax, acetyl-CoA carboxylase, gene cloning, expression analysis, RT-PCR